Fresh Plant Research Articles

Multimodal Data Fusion for Precise Lettuce Phenotype Estimation Using Deep Learning Algorithms

Effective lettuce cultivation requires precise monitoring of growth characteristics, quality assessment, and optimal harvest timing. In a recent study, a deep learning model based on multimodal data fusion was developed to estimate lettuce phenotypic traits accurately. A dual-modal network combining RGB and depth images was designed using an open lettuce dataset. The network incorporated both a feature correction module and a feature fusion module, significantly enhancing the performance in object detection, segmentation, and trait estimation. The model demonstrated high accuracy in estimating key traits, including fresh weight (fw), dry weight (dw), plant height (h), canopy diameter (d), and leaf area (la), achieving an R2 of 0.9732 for fresh weight. Robustness and accuracy were further validated through 5-fold cross-validation, offering a promising approach for future crop phenotyping.

Typha domingensis (Pers.) Poir. ex Steud. Potential to Restore Contaminated Wetlands in Egypt

AbstractSeveral studies have reported Typha domingensis (Typhaceae) as a heavy metal (HM) hyperaccumulator. This study aims to assess the seasonal variations in the accumulation potential of T. domingensis (TD) for HM in polluted wetlands in Greater Cairo, Egypt. Sampling of fresh wild plants from the TD’s subterranean (Root and stem) and surface components were gathered from different polluted sites distributed in the canal of Ismailia and Nahia drain, as well as from the River Nile as an unpolluted site (control). The findings showed that pollution negatively affected the chemical characteristics of sediment and water. Except for the TD’s leaf area, there were significant seasonal variations in the growth characteristics (density, stem diameter, leaf length, and width, fresh and dry biomass). The subterranean parts stored higher amounts of HM than the aboveground shoots. Manganese (Mn) concentration (413.2 mg kg− 1) was greater in the roots of autumn plants, whereas their shoots recorded the highest Pb (225.8 mg kg− 1). Al, Cd, Cu, Fe, Ni, and Zn concentrations in summer plant roots (1499.9, 74.8,1356.1, 3948.8, 403.8, and 1294.2 mgkg− 1, respectively). The factor of metal bioaccumulation (BF) values, was high in TD plants growing in a polluted Ismailia canal, but translocation factor (TF) values for all measured elements were lower than one, except for Pb. Summer is the best season for TD growth to sequester high HM concentrations. The authors recommended that TD be employed to mitigate HM pollution in wetlands and restore them.

Safety and efficacy of a feed additive consisting of Bacillus subtilisDSM 33862 and Lentilactobacillus buchneriDSM 12856 as a silage additive for all animal species (Lactosan GmbH & Co.KG).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on a formulation of Bacillus subtilis DSM 33862 and Lentilactobacillus buchneri DSM 12856 when used as a technological additive to improve ensiling of fresh plant material. The additive is intended for use with fresh plant material with a dry matter (DM) range of 32%-65% at a proposed minimum concentration of 1 × 108 colony forming units (CFU)/kg fresh plant material to be used for all animal species. The bacterial species B. subtilis and L. buchneri are considered by EFSA to be suitable for the qualified presumption of safety approach to safety assessment. The identity of the strains was established, no acquired antimicrobial resistance genes of concern were detected in any of the two active agents and no evidence of toxigenic potential was found in B. subtilis DSM 33862. Therefore, the FEEDAP Panel concluded that the use of the strains as a silage additive is considered safe for all the animal species, for consumers of products from animals fed with the treated silage and for the environment. Regarding user safety, the additive contains microorganisms and therefore should be considered as a potential skin and respiratory sensitiser. The additive is not irritant to skin or eyes. Inhalation and dermal exposure is considered a risk. These conclusions would apply, in principle, to any preparations containing the active agents. The FEEDAP Panel concluded that the additive containing B. subtilis DSM 33862 and L. buchneri DSM 12856 at a minimum concentration of 1 × 108 CFU/kg fresh material has the potential to extend the aerobic stability of silage prepared from fresh plant materials with a DM range of 32%-65%.

Efficacy of the feed additives consisting of Enterococcus faecium ATCC 53519 and E. faecium ATCC 55593 as silage additives for all animal species (FEFANA asbl).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the efficacy of two technological additives (functional group: silage additives) consisting of Enterococcus faecium strains ATCC 53519 and ATCC 55593, respectively. The additives are intended for use with all types of fresh materials and for all animal species at a proposed minimum concentration of 1 × 107 colony-forming units of E. faecium ATCC 53519/kg fresh material or 5 × 106 CFU of E. faecium ATCC 55593/kg fresh material. In a previous opinion, the FEEDAP Panel could not conclude on their efficacy since the dry matter content of the ensiled materials at the end of the experiments was not corrected for volatiles, which led to unreliable estimation of the dry matter loss, and the lack of positive effects on any of the other parameters. The supplementary information submitted by the applicant included updated data on the dry matter loss corrected for volatiles. The results showed that, at the end of the ensiling process, the dry matter loss during the ensiling was significantly lower in the ensiled materials treated with E. faecium ATCC 53519 or ATCC 55593 compared to controls. The FEEDAP Panel concludes that the addition of E. faecium ATCC 53519 or ATCC 55593 in all types of fresh plant materials shows potential to improve the nutrient preservation of silage at the proposed conditions of use.

Phytochemical Characterization Utilizing HS-SPME/GC-MS: Exploration of the Antioxidant and Enzyme Inhibition Properties of Essential Oil from Saudi Artemisia absinthium L.

Background/Objectives: This study aimed to analyze the chemical composition and biological activities of Artemisia absinthium L. essential oil, focusing on its antioxidant and enzyme inhibition (α-amylase and urease) properties. Additionally, in vitro pharmacokinetic and pharmacodynamic evaluations were conducted through in silico molecular docking and BOILED-Egg models to assess its therapeutic potential and its potency in treating oxidative-stress-related diseases. Methods: The essential oil was isolated by the hydrodistillation (HD) of fresh plant material, and volatiles released from dried plant material were sampled via headspace solid-phase microextraction (HS-SPME), followed by a phytochemical profiling analysis through the GC-MS tool. Antioxidant capacity was assessed using DPPH, ABTS, FRAP, and nitric oxide scavenging assays, while enzyme inhibition activities were tested against α-amylase and urease. Molecular docking and BOILED-Egg models were used to evaluate compound interactions with NADPH oxidase and predict pharmacokinetic behavior, respectively. Results: HS-SPME and HD yielded 46 and 25 compounds, respectively, primarily terpenoids represented by camphor (26.4%) and cis-davanone (18.0%) in HS-SPME, while in the HD essential oil, cis-davanone (60.2%) and chamazulene (10.8%) were most prevalent. The antioxidant assays showed a strong activity, with a total antioxidant capacity of 821.8 mg ascorbic acid Eq/gm. The essential oil inhibited urease by 86.7% and α-amylase by 81.8%. Molecular docking showed strong binding affinities with NADPH oxidase, supporting the antioxidant results. Conclusions:A. absinthium EO demonstrated potent antioxidant and enzyme inhibitory activities, suggesting its therapeutic potential for treating enzyme-related disorders like diabetes mellitus and its possible use as a cure for many oxidative-stress-related diseases, thus validating the folkloric use of this plant.

The effect of organic manure on the production of jute (Corchorus olitorius)

Field experiments to investigate the effect of different sources of organic manure (Swine manure (SM), Poultry manure (PM), Cattle manure (CM), Rabbit urine manure (RUM)) on production of Jute (Corchorus olitorius) was carried out. The experimental site was cleared and cultivated (tilled and fertilized). Treatments were laid out before planting at once, there was no re-application of treatment. Data were collected on growth and yield parameters (Foliage, Plant height and Plant fresh weight). There was a significant difference in the foliage of Corchorus olitorius treated with SM, PM, CM, and RUM when compared with the control group. The foliage of Corchorus olitorius with PM treatment is significantly higher (P<0.05) compared with other organic manure treatment except for CM. Significant increase (P<0.05) in the height of Corchorus olitorius was obvious in all the treatment group when compared with control. There is no significant difference (P>0.05) in the height of jute across the organic manure treatment groups (SM, PM and RUM) but there is a significant increase in the plant height of PM when compared CM. SM, PM and RUM significantly increase the fresh weight of the plant when compared with the control and CM. There was a numerical increase in the plant fresh weight of PM when compared to other treatment groups. From this experimental study, PM consistently increased the number of leaves, the fresh plant weight and plant height significantly and it is recommended for Corchorus olitorius production.

Key Soil Abiotic Factors Driving Soil Sickness in Lycium barbarum L. Under Long-Term Monocropping

Sustainable cultivation of Lycium barbarum L. (L. barbarum) in northwest China faces challenges due to soil sickness. While previous studies have explored variations in L. barbarum’s root-associated microbiota, the impact of soil properties on its growth performance and plant–soil feedback remains unclear. This study investigated changes in soil properties across topsoil (0–20 cm) and subsoil (20–40 cm) in primary L. barbarum cultivation regions of northwest China, evaluating seedling growth and plant–soil feedback through pot experiments. Results revealed significantly higher fresh shoot weights in seedlings cultivated in topsoil compared to subsoil, with plant–soil feedback showing an inverse trend. Redundancy analysis indicated positive correlations between both fresh weight and plant–soil feedback with electrical conductivity and dissolved nitrogen content, while negative correlations were observed with soil pH at both depths. Notably, dissolved organic carbon content negatively correlated with fresh weight and plant–soil feedback in topsoil, suggesting a potential relationship between continuous single-species plant litter input and soil sickness under monocropping conditions. These findings indicate that long-term input of a single plant litter type, rather than chemical fertilization, may primarily contribute to L. barbarum soil sickness in northwest China, providing valuable insights for developing sustainable cultivation practices for growing L. barbarum.

Genetic variability, heritability, and genetic advance estimates in selected groundnut genotypes (<i>Arachis hypogaea</i> L.) for agro-morphological and yield traits.

Groundnuts (Arachis hypogaea L.), is a crucial food and oil crop in the tropics, require genetic variability assessment for efficient breeding programs using appropriate parameters like genetic coefficient of variation, heritability estimates, association among yield related traits and genetic advance. This study aims to identify genotypes with desirable traits to improve yield and adaptability, contributing to better crop improvement strategies. The study used a randomized complete block design with three replications, consisting of fourteen selected genotypes. Quantitative traits were used to estimate genetic variability parameters, heritability, and genetic advance. The analysis of variance revealed significant differences (p < 0.005) for all studied traits, indicating greater variability in genotypes. Characters exhibited different levels of variability, heritability, and genetic advance among the genotypes. The estimate of the broad-sense heritability ranged from 13.33% to 33.33% for canopy spread and days to maturity respectively. Low to high phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) were recorded. The study found that traits such as number of branches, pods per plant, fresh and dry plant weight, kernel yield, pod yield, and shelling percentage showed high genotypic coefficient of variation, moderate broad sense heritability, and high genetic advance. These traits can be beneficial for groundnut improvement through effective phenotypic selection, resulting in high expected genetic gain. The study found that pod yield is significantly correlated with kernel yield, grain length, grain width, 100-seed weight, fresh and dry 100-pod weight, and number of pods per plant. Kernel yield is positively correlated with the number of branches per plant, pod per plant, fresh and dry 100-pod weight, 100-seed weight, grain length, and grain width. In addition, the first four principal components accounted for 90.37% of total variation in all morphological traits.

The Effect of LED Lighting Durations on Growth, Yield and Quality of Mustard (Brassica juncea L.) under Greenhouse Condition

Light-emitting diodes (LEDs) show promise as a radiation source for intensive crop cultivation systems, especially for vegetables in the greenhouse. Regarding mustard greens, most studies have investigated the effects of LED lights at the seedling stage under chamber conditions for microgreens not addressed throughout their growing period up to harvest. Therefore, this study is focused to evaluate the effect of additional LED lighting time from 6 pm on the growth, yield and quality of mustard greens under a greenhouse in Kien Giang province as a typical tropical region. The experiment was arranged in a completely randomized design with 3 replications, from August 1st to September 18th, 2023. Four treatments: AL0, AL2, AL4 and AL6 correspond to lighting durations of 0, 2, 4 and 6 h of LED light, which includes a combination of 3 white lights: 1 pink light, 58 μmol·m−2·s−1 PPFD, and a Blue (18 %): Green (41 %): Red (41 %) ratio. Results showed that AL4 had the most significant positive impact on mustard plant growth, with higher dry and fresh weight, plant height, leaf width and length. However, AL6 inhibited most parameters except leaf number. Additionally, under AL4 conditions, mustard allocated more biomass to leaves, while those under AL6 conditions allocated more biomass to stems. In terms of quality, LED lighting duration negatively influenced protein content. For instance, AL0 showed the significantly greatest protein value (2.87 ± 0.08 g·kg−1) compared to others. Apart from AL0, AL6 had a considerably higher value of protein content (2.58 ± 0.2 g·kg−1) compared to AL2 (1.74 ± 0.04 g·kg−1) and AL4 (2.17 ± 0.07 g·kg−1). The additional durations of LED lighting influenced mature mustard greens’ morphology, physiology and quality, providing valuable scientific information for improving vegetable quality and quantity in tropical zones. HIGHLIGHTS 4 hours of additional LED light had the most significant positive impact on mustard plant growth, with higher dry and fresh weight, plant height, leaf width, and length. Additionally, leaf number increased under 6 hours of additional LED light. Under 4 hours of additional LED light, mustard allocated more biomass to leaves, while those under 6 hours of additional LED light allocated more biomass to stems. Lighting with LED resulted in a lower protein value, and different durations of LED lighting resulted in varying protein content values. GRAPHICAL ABSTRACT

Влияние некоторых видов обработки на продолжительность сушки и органолептические показатели нарезанных баклажанов

Abstract. Eggplant is a common vegetable crop from the Solanaceae family, whose share of global vegetable harvests in 2022 was about 5 %. However, eggplant is thermophylic and its fruits cannot withstand low temperatures due to physiological disorders and quality reduction. This fact and thin covering tissues make the shelf life of eggplant fruits short. For this reason, improving existing methods for preserving the quality and extending the period of use of eggplants is relevant. One of the most popular methods of preserving fresh plant raw materials is drying, but it requires the selection of a method (including pre-treatment of raw materials) and process parameters to preserve the quality of dried products. In the course of the study, the influence of certain types of physical and chemical pre-treatment (microwave frequencies, citric acid, sodium chloride, their combinations) on the drying time and organoleptic characteristics of sliced eggplant fruits of the Chernomor variety was studied. It was found that various types of treatments used influenced the duration of drying of eggplants: treatments with microwave frequencies and citric acid led to a reduction in process time by 31-43 minutes compared to the control; treatments with sodium chloride solution and complex treatments, on the contrary, increased the duration by more than 3 hours. Organoleptic evaluation of dried eggplants showed that the best characteristics were provided by treatment with 1% and 5% citric acid solutions. While pre-treatment with microwave frequencies and NaCl with various parameters gave an unpleasant taste and was accompanied by darkening of the product. The data obtained can be used to improve existing methods of drying eggplants. Key words: eggplants, drying, organoleptic characteristics, super high frequency, citric acid, sodium chloride

Safety and efficacy of a feed additive consisting of Lentilactobacillus buchneriDSM 32651 for all animal species (BioCC OÜ).

Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of Lentilactobacillus buchneri DSM 32651 when used as a technological additive to improve ensiling of fresh plant material. The additive is intended for use in all fresh material for all animal species at a proposed minimum concentration of 1 × 108 colony forming units (CFU)/kg fresh plant material. The bacterial species L. buchneri is considered by EFSA to be suitable for the qualified presumption of safety approach to safety assessment. The identity of the strain was established and no acquired antimicrobial resistance genes of concern were detected. Therefore, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) concluded that the use of the strain as a silage additive is considered safe for all the animal species, for consumers of products from animals fed the treated silage and for the environment. Regarding user safety, the additive should be considered as a potential skin and respiratory sensitiser, and any exposure through skin and respiratory tract is considered a risk. The additive is not an eye irritant. The FEEDAP Panel concluded that the additive consisting of L. buchneri DSM 32651 at a minimum concentration of 1 × 108 CFU/kg fresh material may extend the aerobic stability of silage prepared from fresh plant material with a DM range of 28%-45%.

Impact of Fresh Khat Extract on Proinflammatory Cytokines and Hepatic and Renal Responses.

BACKGROUND This study examined the effects of khat extract on the proinflammatory cytokines, liver, and kidneys of rats. Unlike previous research that focused on broad immune markers and general effects, this study investigated specific proinflammatory cytokines (IL-1ß, IL-2, IL-4, IL-6, and TNF-alpha) and considered gender differences in the immune response. Fresh khat plants and nontoxic doses were used to obtain clear observations relevant to human health. MATERIAL AND METHODS Extracts were prepared from young shoots of khat plants and fresh leaves. 150-gram male and female rats were randomly divided into 8 groups (5 rats per group). Six groups received the extract orally for 8 weeks at doses of 100, 300, and 500 mg/kg/day. Blood samples were collected after 56 days and assayed for cytokines using ELISA, while liver enzymes and kidney markers were assayed using kinetics and colorimetric methods. RESULTS Khat extract increased the levels of most cytokines, with higher doses causing greater increase. This effect was consistent across sexes for some cytokines (IL-1ß, IL-2), but not others (IL-4, IL-6, TNF-alpha), where sex played a role at specific doses. Various doses of the extract influenced glucose levels in both male and female rats. In addition, liver enzyme markers showed some variations. CONCLUSIONS This study, with its intriguing findings, illuminates the complex effects of khat on various systems. Variations in AST and ALT levels in the liver support earlier hepatotoxicity research and suggest possible khat-induced damage. This study revealed consistently increased levels of all cytokines as the dose of khat extract increased.

Effect of cotton seed size and seeding density on cotton growth, development, and yield

AbstractInterest in cotton seed size and seeding density exists due to increased seeding cost and overall decreased seed size of cotton varieties. An experiment was conducted in 2019 and 2020 in Jackson, TN, Starkville, MS, and Brooksville, MS, to determine the impact of seed size, seeding density, and variety on cotton plant development and yield. Early‐season seedling vigor was impacted by seeding density and seed size. Larger seeds and higher seeding densities produced the greatest seedling vigor. Fresh weight biomass was also impacted by seed size, as larger seed produced greater fresh and dry cotton plant biomass when pooled over seeding density and variety. The greatest seed cotton yields were obtained from planting larger seed, higher seeding densities, and from ‘DP 1646 B2XF’. Cotton variety and seeding density influenced financial returns and fiber quality. ‘NexGen 3406 B2XF’ planted at 148,200 seeds ha−1 resulted in the lowest micronaire. Net returns were not influenced by seed size or seeding density; therefore, depending on seed costs, increasing seeding densities may not be beneficial.

Carbon dot unravels accumulation of triterpenoid in Evolvulus alsinoides hairy roots culture by stimulating growth, redox reactions and ANN machine learning model prediction of metabolic stress response

Evolvulus alsinoides, a therapeutically valuable shrub can provide consistent supply of secondary metabolites (SM) with pharmaceutical significance. Nonetheless, because of its short life cycle, fresh plant material for research and medicinal diagnostics is severely scarce throughout the year. The effects of exogenous carbon quantum dot (CD) application on metabolic profiles, machine learning (ML) prediction of metabolic stress response, and SM yields in hairy root cultures of E. alsinoides were investigated and quantified. The range of the particle size distribution of the CDs was between 3 and 7 nm. The CDs EPR signal and spin trapping experiments demonstrated the formation of O2-•spin-adducts at (g = 2.0023). Carbon dot treatment increased the levels of hydrogen peroxide and malondialdehyde concentrations as well as increased antioxidant enzyme activity. CD treatments (6 μg mL-1) significantly enhanced the accumulation of squalene and stigmasterol (7 and 5-fold respectively). The multilayer perceptron (MLP) algorithm demonstrated remarkable prediction accuracy (MSE value = 1.99E-03 and R2 = 0.99939) in both the training and testing sets for modelling. Based on the prediction, the maximum oxidative stress index and enzymatic activities were highest in the medium supplemented with 10 μg mL-1 CDs. The outcome of this study indicated that, for the first time, using CD could serve as a novel elicitor for the production of valuable SM. MLP may also be used as a forward-thinking tool to optimize and predict SM with high pharmaceutical significance. This study would be a touchstone for understanding the use of ML and luminescent nanomaterials in the production and commercialization of important SM.

LC-HRMS Analysis of Abelmoschus Manihot Medik from Palu of Central Sulawesi

Abelmoschus manihot (L.) Medik is one of the traditional medicinal plants from Palu City, Central of Sulawesi with the Malvaceae plant family and commonly is a traditional medicinal plants recognized fot its therapeutic potential, particularly in antioxidant, anti-inflammatory and wound healing applications. This research aims to identify and characterize the chemical compounds present in Abelmoschus manihot (L.) Medik plant from Palu City, Central of Sulawesi using liquid chromatography high resolution mass spectrometry (LC-HRMS). This research was collected fresh plant, designed using ethanol extraction from Abelmoschus manihot (L.) Medik and analyzed using mass spectroscopy (LC-HRMS) with Processing Software – MZMine Ver. 3.9.0. The results of this study provide new insights into the chemical composition of Abelmoschus manihot (L.) Medik and confirm its potential as a source of bioactive compounds that could be futher explored for pharmaceutical development, namely that it contains the main compound Quercetin with a mass of 303.04, Gossypetin; Myricetin with a mass of 319.04, Quercetin 3-O-alpha-L-rhamnoside with a mass of 449.10 Isoquercetin; Hyperoside; Hyperin; Hirsutrin with a mass of 465.10, Gossypol with a mass of 519.20 and rutin with a mass of 291.08.

Reduction of Cr(VI) by Bacillus toyonensis LBA36 and its effect on radish seedlings under Cr(VI) stress.

Chromium, being among the most toxic heavy metals, continues to demand immediate attention in the remediation of Cr-contaminated environments. In this study, a strain of LBA36 (Bacillus toyonensis) was isolated from heavy metal contaminated soil in Luanchuan County, Luoyang City, China. The reduction and adsorption rates of LBA36 in 30 mg·L-1 Cr-containing medium were 97.95% and 8.8%, respectively. The reduction mechanism was confirmed by Fourier-transform infrared spectroscopy, and X-ray photoelectron spectroscopy (XPS). Cr(VI) reduction by this strain predominantly occurred outside the cell, with hydroxyl, amide, carboxyl, C-N group, carbonyl, and sulfur carbonyl as the main reaction sites. XPS analysis revealed the presence of Cr2p1/2 and Cr2p3/2. Furthermore, the hydroponic experiment showed that the fresh weight and plant height of radish seedlings increased by 87.87% and 37.07%, respectively, after inoculation with LBA36 strain under 7 mg·L-1 Cr(VI) stress. The levels of chlorophyll, total protein, malondialdehyde, superoxide dismutase and catalase were also affected to different degrees. In conclusion, this study demonstrated the potential of microbial and phytoremediation in the treatment of heavy metal toxicity, and laid the foundation for the development of effective bioremediation methods for Cr(VI) pollution.

The value of patch testing with plants "as is" in diagnosing plant sensitization.

The number of commercially available plant allergens/extracts is limited and therefore patch testing with fresh/dried plant material may be a necessary supplement in diagnosing plant allergy. To evaluate the usefulness of patch testing with plants "as is" compared to patch testing with commercial and in-house produced plant test materials and to report on species eliciting positive patch test reactions. Consecutive eczema patients, who were patch tested between January 2019 and December 2023 and who had at least one positive reaction to a plant allergen and/or extract and/or plant "as is" were included in the study. A total 57 out of 1893 patients tested (3%) were sensitised to plants. Compositae plants were the most frequent sensitizers, followed by tomato, tulipalin A, falcarinol, and Philodendron plants. In 12 patients (21%), the diagnosis was based on patch testing with fresh plants only. Occupational sensitization occurred in 32%. Other sensitizers included Hydrangea, Pelargonium zonale, and Monstera. A large minority of plant-sensitised patients would have been undiagnosed without patch testing with plants "as is." Most of the culprit plants were known sensitizers, but not commercially available, and these and new species taken into cultivation makes patch testing with fresh plants unavoidable and worthwhile.

Cistus libanotis from Algeria: Phytochemical analysis by GC/MS, HS-SPME-GC/MS, LC–MS/MS and its anticancer activity

In this paper, Cistus libanotis (Cistus clusii Dunal) from Algeria was investigated. This plant is utilized in numerous industries, mostly in perfumery and, more recently, as a raw material for dietary supplements. For the first time, the present study evaluated the anticancer potential of Algerian C. libanotis by performing a comprehensive phytochemical investigation. The volatile fraction of the fresh plant material of C. libanotis was investigated using headspace solid-phase microextraction (HS-SPME) coupled to GC-MS, and the chemical composition of the essential oil (EO) of C. libanotis was examined using GC-MS technique; additionally, the phenolic compounds were identified and quantified using liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). A healthy cell line (L929) and two cancer cell lines (CAPAN-1 and dld-1) were used to test the ethanolic extract's cytotoxicity. HS-SPME-GC/MS analysis identified sabinene (25.3 %) and α-thujene (23.8 %) as principal components. In EO the major ingredients were γ-terpinene (17.4 %), camphene (16.13 %), α-phellandrene (15.36 %), and 4-carene (13.54 %). Nevertheless, 24 components were identified by LC-ESI-MS/MS analysis and shikimic acid, luteolin and hesperidin were most abundant. Further, the ethanolic extract of C. libanotis showed a significant cytotoxic effect against dld-1 and CAPAN-1 cell lines, while it was less active against the healthy cell line L929.

Effects of foliar and root application of Zn and Fe bio-nanofertilizers on the glucosinolate, Zn and Fe contents of Pak choi (Brassica rapa Subsp. Chinensis) grown under hydroponic and pot cultivation

Pak choi is a rich source of minerals and health beneficial compounds such as glucosinolates (GSLs). This study is focused on implementing sustainable agronomic approaches to optimize pre-harvest cultivation and improve plant growth and nutritional value. Therefore, the aim of this work was to evaluate the effect of foliar and root fertilization of algae capped ZnO and γ-Fe2O3 nanoparticles on the growth and content of GSLs, Zn and Fe of Pak choi grown under hydroponic floating system and pot conditions. The foliar application of ZnO (77 nm) and γ-Fe2O3 (68 nm) nanoparticles functionalized with an Arthrospira platensis extract increased fresh weight (39.71 %), plant height (42.77 %), leaves number (26.47 %), root length (33.33 %), and Zn (10-fold) and Fe (2-fold) contents in hydroponic plants. The same treatment also improved plant fresh weight (31.73 %), and Zn (6-fold) and Fe (2-fold) in pot cultivated Pak choi. Six GSLs were detected, which were also enhanced by the joint application of nanoparticles and microalgae extract. The results revealed a high synergy between the ZnO- and γ-Fe2O3 nanoparticles and the A. platensis extract to increase plant growth and Zn and Fe micronutrients and glucosinolates in Pak choi grown under hydroponic and pot systems.

Effect of Nitrogen and Humic Acid on Growth and Yield of Pakcoy (Brassica rapa subsp. chinensis) Plants

Decreased soil quality due to reduced nutrient content is one of the causes of decreasedquality and yield in plants. The use of humic acid organic matter with N fertilizer is one of the effortsto increase nutrients in the soil while increasing plant growth and yield. This research was conductedin Heuleut Village, Leuwimunding District, Majalengka Regency, from May to June 2024. Theresearch method used was the experimental method with a group-randomized design. The treatmentconsisted of 16 treatments that were repeated twice. The variables observed were plant height,number of leaves, root volume, plant fresh weight, leaf area, and plant growth rate. The resultsshowed that the use of N fertilizer at a dose of 115 kg/ha (urea 250 kg/ha) and humic acid 3 g/planthad a good effect on all variables observed. The results of soil analysis showed an increase in N-totaland C-organic.