Fresh Chicken Breast Research Articles

Effect of salt-induced supercooling stability on the quality of chicken breast during supercooled storage

The effect of salt on the stability and quality of fresh chicken breast meat during supercooling preservation was investigated. The results showed that 0.75% salt kept the non-frozen state of chicken breast at −3 °C for 12 days. Compared to storage at −1 °C, storing at −3 °C could further extend the shelf life of chicken, but there was a risk of freezing the chicken meat (31% frozen after 4 d and all after 8 d). Microstructure images revealed that muscle fibers in the −3 °C samples were destroyed by crystallization, whereas ice nucleation was not observed in the −3 °C + salt treatment. The drip loss and centrifuging loss of the −3 °C + salt group after 12 days of storage (1.48%, 20.10%) were significantly lower than those of the −1 °C group (4.14%, 24.49%), the −3 °C group (8.37%, 27.47%), and the −1 °C + salt group (1.99%, 22.60%). Low-field nuclear magnetic resonance results showed that water migration in chicken breast was inhibited with −3 °C + salt treatment. Overall, the good quality of chicken breast was maintained by −3 °C + salt. The results provide a theoretical basis for industrial application of supercooling preservation of chicken.

Development of antifouling antibacterial polylactic acid (PLA) -based packaging and application for chicken meat preservation

Microbial contamination is the leading cause of food spoilage and food-borne disease. Here, we developed a multifunctional surface based on polylactic acid (PLA) bioplastic with antifouling and antibacterial properties via a facile dual-coating approach. The surface was designed with hierarchical micro/nano-scale roughness and low surface energy. Bactericidal agent polyhexamethylene guanidine hydrochloride (PHMG) was incorporated to endow the film with bactericidal activity. The film had good superhydrophobic, antifouling and antibacterial performance, with a water contact angle of 154.3°, antibacterial efficiency against E. coli and S. aureus of 99.9 % and 99.6 %, respectively, and biofilm inhibition against E. coli and S. aureus of 63.5 % and 68.9 %, respectively. Synergistic effects of antibacterial adhesion and contact killing of bacteria contributed to the significant antibacterial performance of the film. The biobased biodegradable film was highly effective in preventing microbial growth when applied as antibacterial food packaging for poultry product, extending the shelf life of fresh chicken breast up to eight days.

Salmonella Inactivation Model by UV-C Light Treatment in Chicken Breast.

This study aims to evaluate the effectiveness of inactivating Salmonella enteritidis in fresh chicken breast by irradiation using a combination of short-wave UV (0, 3, 6, 9, 12, and 15 J/cm2) and a natural antimicrobial such as caffeine (0, 5, 10, 15, and 20 nM/g) at 14 °C as alternative proposals to conventional techniques to reduce pathogens in food. The effect of temperature was studied in an initial phase (2 to 22 °C). The most suitable models were double Weibull in 60% of cases, with an adjustment of R2 0.9903-0.9553, and Weibull + tail in 46.67%, with an adjustment of R2 of 0.9998-0.9981. The most effective combination for the reduction in Salmonella was 12 J/cm2 of UV light and 15 nM/g of caffeine, with a reduction of 6 CFU/g and an inactivation rate of 0.72. The synergistic effect was observed by increasing caffeine and UV light. Furthermore, the physico-chemical characteristics of the food matrix were not affected by the combination of both technologies. Therefore, these results suggest that this combination can be used in the food industry to effectively inactivate Salmonella enteritidis without deteriorating product quality.

Sustainable gelatin-based packaging with nanoemulsified chilli seed oil for enhancing poultry meat preservation: An eco-friendly approach

Utilizing poultry feet, a typically underutilized by-product, we extracted gelatin to fabricate environmentally friendly packaging pouches for the shelf life enhancement of fresh chicken breast cubes. The incorporation of chilli seed oil nanoemulsions, characterized by their particle sizes (61-127 nm) and zeta potential values (-18 to -28 mV), not only contributed to the pouches' structural integrity but also imparted antimicrobial properties. These pouches demonstrated reduced UV light transmittance and controlled color changes in packaged chicken breast cubes, compared to the control samples. Significantly, the pouch-packaged samples exhibited slower pH increase, reduced weight loss (16.2-20.4%), and lower TBARS values, indicative of delayed lipid oxidation and spoilage. Microbiological assessments revealed that the packaged samples effectively restrained microbial growth, staying within safety limits for E. coli and total plate counts, unlike the control samples which exceeded these thresholds. Textural analysis further showed better preservation of the meat's consistency within the pouches. This investigation not only highlights the valorization of food industry by-products in developing sustainable packaging solutions but also emphasizes the efficiency of developed packaging pouches in enhancing shelf-life of fresh poultry meat cubes, thus resonating with the contemporary needs of food processing technologies and consumer acceptance.

The selective extraction of dietary polyamines from chicken breast using the application of a lab-on-a-chip electromembrane and dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry.

Excessive dietary polyamines (PAs), including putrescine (PUT), spermine (SPM), and spermidine (SPD), have become a worldwide concern due to their carcinogenicity and reduced shelf life. A modern miniaturized on-chip electromembrane extraction (EME) has been applied to extract these compounds from chicken breast samples. This method is based fundamentally on ionic compounds' electrostatic attraction, diffusion, and solubility in the acceptor phase. The chemical structure of polyamines enables their efficient extraction using an electric driving force on a microchip device. HCl solution (0.1 mol L-1) was applied as an aqueous acceptor solvent. Dispersive liquid-liquid microextraction was performed after EME to facilitate joining three-phase EME to GC-MS and improve the merit figures. The total ranges of 3.77-7.89 μg g-1, 3.48-7.02 μg g-1, and 0.78-2.20 μg g-1 were acquired as PUT, SPM and SPD concentrations in chicken breast, respectively. The results demonstrate that the level of PAs in fresh chicken breast samples is not concerning, but it may reduce the quality of chicken meat over time. This novel analytical technique has several advantages: high recovery, substantial quickness, remarkable selectivity, and good enrichment factors. This emerging method could be generalized to other studies to analyze different foodstuffs.

Comparison of the impact of UV-light emitting diode and UV lamp at pilot-plant scale level on quality parameters and consumer perception of fresh chicken meat

The present study compared the impact of two UV light devices: conventional UV lamp and UV-LED on the colour, pH, lipid and protein oxidation of fresh chicken breast meat aerobically stored at 4 °C for 10 days. Lipid oxidation was the most impacted quality attribute in UV lamp treated meat, unlike UV-LED that showed no effect compared to non-treated meat. Slight changes were observed in colour, pH and protein oxidation of chicken samples subjected to UV lamp and UV-LED. To evaluate these changes from a consumer perspective, the different treatment samples were stored at 4 °C for 3 days and colour likeness, odour likeness and overall appearance were assessed by consumer sensory analysis. However, alterations in quality parameters of chicken meat caused by UV light did not decrease overall acceptance in the sensory analysis. UV-LED was the preferred chicken meat by the participants, even compared to non-treated meat.

Widespread Multidrug Resistance of Arcobacter butzleri Isolated from Clinical and Food Sources in Central Italy.

The Arcobacter genus comprises a group of bacteria widely distributed in different habitats that can be spread throughout the food chain. Fluoroquinolones and aminoglycosides represent the most common antimicrobial agents used for the treatment of Arcobacter infections. However, the increasing trend of the antimicrobial resistance of this pathogen leads to treatment failures. Moreover, the test implementation and interpretation are hindered by the lack of reference protocols and standard interpretive criteria. The purpose of our study was to assess the antibiotic resistance pattern of 17 A. butzleri strains isolated in Central Italy from fresh vegetables, sushi, chicken breast, and clinical human samples to provide new and updated information about the antimicrobial resistance epidemiology of this species. Antimicrobial susceptibility testing was carried out by the European Committee on Antimicrobial Susceptibility Testing (EUCAST)'s disc diffusion method. All the strains were multidrug resistant, with 100% resistance to tetracyclines and cefotaxime (third generation cephalosporins). Some differences were noticed among the strains, according to the isolation source (clinical isolates, food of animal origin, or fresh vegetables), with a higher sensitivity to streptomycin detected only in the strains isolated from fresh vegetables. Our data, together with other epidemiological information at the national or European Union (EU) level, may contribute to developing homogeneous breakpoints. However, the high prevalence of resistance to a wide range of antimicrobial classes makes this microorganism a threat to human health and suggests that its monitoring should be considered by authorities designated for food safety.

Alginate-based edible coating with oregano essential oil/β-cyclodextrin inclusion complex for chicken breast preservation

A sodium alginate (SA) edible coating containing oregano essential oil (OEO)/β-cyclodextrin (β-CD) inclusion complexes (SA/OEO-MP coating) was developed to extend the shelf life of fresh chicken breast during refrigeration storage. First, OEO was inserted into the hydrophobic interior of β-CD to form an inclusion complex (OEO-MP) that maintained its excellent antioxidant and antibacterial activities. The formed OEO-MP was characterized using fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). In addition, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) results demonstrated that β-CD could improve the thermal stability of OEO. The encapsulation efficiency reached 71.6 %, and OEO was released continuously from the OEO-MP. The lipid oxidation, total viable count (TVC) and sensory properties of chicken breasts were regularly monitored when OEO-MP was incorporated into the SA coating for chicken breast preservation. Compared with the uncoated group, the SA/OEO-MP-coated groups showed significantly reduced increases in pH, thiobarbituric acid reactive substances (TBARS), total volatile base nitrogen (TVB-N), and TVC, especially in the SA/OEO-MP1 group. In summary, the SA/OEO-MP coating could preserve the chicken breast by reducing lipid oxidation and inhibiting the proliferation of microorganisms. It would be developed as a prospective edible packaging for chicken preservation.

The two-stage air thawing based on low voltage electric field (LVEF) can make the quality of thawed chicken breast close to that before freezing

Low voltage electric field (LVEF) thawing has been shown to be an effective method of thawing. In this study, chicken breast meat was thawed in a fixed electric field voltage (2.5 KV), and the thawing process was divided into two stages: stage Ⅰ (−20 °C to −5 °C) and stage Ⅱ (−5 °C to −1 °C). The effects of two-stage air thawing based on low voltage electric field (TLT) on the quality of chicken breast were investigated by altering the thawing temperature (T1 and T2) in two stages. The results showed that TLT could significantly (P＜0.05) reduce thawing time and thawing loss than air thawing. Among them, TLT22.5–7.5 samples almost retained the same tissue structure as fresh chicken breast, allowing the muscle fibers/bundles to capture more immobile water. TLT22.5–7.5 samples not only kept the color similar to fresh meat, but also had an obviously better moisture correlation characteristic than other thawed samples. TLT also demonstrated a strong microbial reproduction inhibitory impact. The thawed chicken could obtain a comparatively fresh quality if T2 was kept below 15 °C. Therefore, TLT is expected to reduce total thawing time and prevent meat quality deterioration caused by the freezing and thawing process.

Comparing the Effects of Thyme (Zataria multiflora) and Rosemary (Rosmarinus officinalis) Essential Oils on Microbiological, Physicochemical, and Sensory Properties of Vacuum-Packaged and Refrigerated Chicken Breast

This study aimed to compare the effects of thyme and rosemary essential oils (EOs) with vacuum packaging on shelf life extension and organoleptic properties of chicken breast meat. For this purpose, two concentrations of 0.1% and 0.3% (v/w) from thyme and rosemary EOs were added individually to fresh chicken breast samples, packed under vacuum conditions, and stored at 4°C for 12 days. All the samples were evaluated for microbiological, physicochemical, and sensory properties at 3-day intervals. The results showed that the thyme EO treatments, especially at 0.3% concentration, significantly reduced both total and psychrotrophic colony counts, and they were more effective in reducing microbial counts compared to rosemary EO treatments. Moreover, thyme EO significantly controlled Escherichia coli growth in treated samples. TBA values in treated samples with thyme EOs were significantly less than in rosemary EOs and control samples. Results indicated that rosemary EO treatments had the lowest pH during 12 days. Colorimetric and organoleptic analysis of the samples showed statistical differences on various days. In the EOs-treated chicken breasts, the color and texture of the samples improved compared to controls, but the taste and odor were not desired at the higher concentration of thyme and rosemary EOs.

Novel Bionanocomposites Based on Cinnamon Nanoemulsion and TiO2-NPs for Preserving Fresh Chicken Breast Fillets

In this study, bionanocomposite coating solutions were created using polyvinyl alcohol (PVA) and chitosan (Cs), with different concentrations of cinnamon essential oil in nanoemulsion (n-CEO; 0%, 5%, 10%, and 20%) and TiO2 nanoparticles (TiO2-NPs). The bionanocomposite was characterized using Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy with EDX, and mechanical and barrier property assessment. Additionally, antimicrobial and antioxidant properties and total phenols were evaluated. Generally, mechanical and barrier properties were enhanced with increasing n-CEO concentrations with a favorable distribution in film matrix. Moreover, total phenols, antioxidant, and antimicrobial activities were also enhanced a broader inhibition pattern against A. flavus, gram-positive, and gram-negative bacteria. The influence of n-CEO and TiO2-NPs blended into bionanocomposite on preservation of fresh chicken breast fillets during 21 days of refrigeration was evaluated. Added n-CEO concentration, especially 20%, and TiO2-NPs enhanced antimicrobial properties and extended preservation time up to 14 days compared to uncoated samples. Furthermore, weight loss was decreased during storage of coated samples. Thus, PVA/Cs/TiO2–NPs with n-CEO bionanocomposites may be useful as a coating for chicken breast fillets to control microbial growth and reduce weight loss during cold storage.

Development and comparison of classification models on VIS-NIR hyperspectral imaging spectra for qualitative detection of the Staphylococcus aureus in fresh chicken breast

Chicken is at risk of contaminated foodborne pathogens in the production process. Timely and nondestructive detection of foodborne pathogens in chicken is essential for food security. The study aims to explore the feasibility of developing efficient classification models for qualitative detection of Staphylococcus aureus in chicken breast using the hyperspectral imaging technique. Principal component analysis was used to process the full spectral information and three wavelength selection methods (competitive adaptive reweighted sampling, genetic algorithm, and successive projections algorithm) were applied to extract effective wavelengths. These methods were combined with the support vector machine algorithm to develop conventional classification models, respectively. In addition, a convolutional neural network model based on deep learning was designed and trained for comparison. The performance of the convolutional neural network model was significantly better than that of conventional classification models. The overall accuracy for chicken sample classifications was improved from 83.88% to 91.38%. The results demonstrated that deep learning can effectively extract spectral features and promote the application of hyperspectral imaging in foodborne pathogens detection of chicken products.

Development of curcumin incorporated composite films based on chitin and glucan complexes extracted from Agaricus bisporus for active packaging of chicken breast meat

Composite films were prepared by combining different concentrations of curcumin with chitin and glucan complexes (CGCs) extracted from Agaricus bisporus via a solution casting method. The developed curcumin doped CGC (CGC/Cu) films were characterized in terms of surface, optical, structural, barrier, mechanical, antioxidant, and antimicrobial properties. The biodegradability of CGC/Cu films was determined in soil for 14 days. The incorporation of curcumin significantly affected the surface morphology and improved light barrier properties, radical scavenging activity, and total phenolic content of the films. The CGC/Cu films containing different concentrations of curcumin showed antibacterial activity against Escherichia coli, while antibacterial activity against Staphylococcus aureus was not observed with the developed films. Afterward, the microbial properties of the fresh chicken breast were examined during refrigerated storage for 10 days. The shelf-life of chicken samples wrapped in the developed film was extended at least 40 % compared to the control sample. In conclusion, curcumin incorporated CGC based films can serve as a promising biodegradable active packaging material to improve the shelf-life of meat products.

Antibacterial effect of edible coatings with essential oil

Food preservation technologies are a continuously renewed area because of industrial and customer needs, social transformation and environmentally friendly processing and climate change. The shelf life of perishable food products must be extended with different technologies, for example using green methods like the edible coating (EC). EC is made from different biopolymers (chitosan, alginate, gelatine, agar), the effect can increase with using plant extracts. In this study, the effect of chitosan EC, chitosan EC+thyme essential oil (EO); effect of alginate EC, alginate EC+thyme EO was examined on fresh chicken breast having artificial contamination with Escherichia coli; Enterococcus faecalis, that the EC can extend the shelf life. The organoleptic quality of baked treated chicken breast was established also. Based on the result both EC can decrease the cell number (with 1-3 log CFU/g) on treated chicken breast and this antimicrobial effect was enhanced with thyme essential oil (3.2 µl/ml concentration). There was significant differences (p<0.05) between the two edible coatings. Alginate had better preservation effect, than chitosan. However, the thyme EO could increase the antimicrobial activity of chitosan in higher values, than the effect of alginate EC. In this experiment, the E. faecalis was more sensitive to treatment than the E. coli. In conclusion, the edible coating can be used as an alternative preservation technique and these combined with essential oils can extend the shelf life of chicken breast fillet.

Effect of a carrageenan/chitosan coating with allyl isothiocyanate on microbial load in chicken breast

This study investigated the effectiveness of carrageenan/chitosan coating in combination with allyl isothiocyanate (AITC) against Salmonella Typhimurium and Campylobacter coli on fresh chicken breasts. High (8–7 log10 CFU/g) and low (6–5 log10 CFU/g) inoculation levels represented extreme and average contamination, respectively. When used in combination with the coating, treatment with 200 μg/mL AITC reduced (P ≤ 0.05) S. Typhimurium populations by 1.11 and 1.79 log10 CFU/g in the high and low inoculation samples, respectively, after 21 days of storage at 4 °C. Populations of C. coli were reduced (P ≤ 0.05) below the detection limit (0.3 log10 CFU/g) in the high and low inoculation samples treated with the coating and 20 μg/mL of AITC after 21 days of storage at 4 °C. S. Typhimurium exposed to 200 μg/mL AITC showed increased (P ≤ 0.05) resistance to AITC after the 21-day exposure period; no change (P > 0.05) was observed for C. coli exposed to 20 μg/mL of AITC . A carrageenan/chitosan coating combined with a 200 μg/mL or 20 μg/mL AITC treatment reduces S. Typhimurium and C. coli populations, respectively, thus showing potential as an antimicrobial additive to enhance microbiological safety of fresh poultry.

Efficacy of Biopolymer/Starch Based Antimicrobial Packaging for Chicken Breast Fillets.

Food contamination leading to the spoilage and growth of undesirable bacteria, which can occur at any stage along the food chain, is a significant problem in the food industry. In the present work, biopolymer polybutylene succinate (PBS) and polybutylene succinate/tapioca starch (PBS/TPS) films incorporating Biomaster-silver (BM) and SANAFOR® (SAN) were prepared and tested as food packaging to improve the lifespan of fresh chicken breast fillets when kept in a chiller for seven days. The incorporation of BM and SAN into both films demonstrated antimicrobial activity and could prolong the storability of chicken breast fillets until day 7. However, PBS + SAN 2%, PBS/TPS + SAN 1%, and PBS/TPS + SAN 2% films showed the lowest microbial log growth. In quality assessment, incorporation of BM and SAN into both film types enhanced the quality of the chicken breast fillets. However, PBS + SAN 1% film showed the most notable enhancement of chicken breast fillet quality, as it minimized color variation, slowed pH increment, decreased weight loss, and decelerated the hardening process of the chicken breast fillets. Therefore, we suggest that the PBS + SAN and PBS/TPS + SAN films produced in this work have potential use as antimicrobial packaging in the future.

Effect of UVC Light-Emitting Diodes on Pathogenic Bacteria and Quality Attributes of Chicken Breast

This study was conducted to investigate the inactivation of foodborne pathogens and the quality characteristics of fresh chicken breasts after UVC light-emitting diode (UVC-LED) treatment. Fresh chicken breasts were separately inoculated with Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes at initial populations of 6.01, 5.80, and 6.22 log CFU/cm2, respectively, and then treated with UVC-LED irradiation at 1,000 to 4,000 mJ/cm2. UVC-LED irradiation inactivated the test bacteria in a dose-dependent manner. After UVC-LED treatment at 4,000 mJ/cm2, the populations of Salmonella Typhimurium, E. coli O157:H7, and L. monocytogenes on chicken breasts were decreased by 1.90, 2.25, and 2.18 log CFU/cm2, respectively. No significant changes (P > 0.05) were found in color, pH, texture, and thiobarbituric acid-reactive substances of chicken breasts following UVC-LED irradiation at doses ≤4,000 mJ/cm2. These results indicate that UVC-LED radiation is a promising technology for reducing the level of microorganisms while maintaining the physicochemical characteristics of poultry meat.

Nano-encapsulation of pomegranate (Punica granatum L.) peel extract and evaluation of its antimicrobial properties on coated chicken meat

Pomegranate peel extract (PPE) is a valuable source of bioactive compounds, with antimicrobial activity. On the other hand, reducing the extract particle size to a nano-scale due to the increase in the surface-to-volume ratio can increase its effectiveness. The alginate nanospheres containing PPE were prepared through water in oil (W/O) emulsification and external gelation with calcium chloride nanoparticles. The optimum ratios for alginate:PPE and alginate:calcium chloride nanoparticles, based on encapsulation efficiency and nanospheres size, were 4:1 and 9:1, respectively (p < 0.05). The particle size and encapsulation efficiency of optimum nano-capsules containing PPE were 205.1 ± 0.1 nm and 83.90 ± 0.53%, respectively. The morphology and physical stability of optimum nano-capsules were further studied. After preparation of PPE-loaded nano-particles, the antimicrobial activity of the nanospheres containing 200 mg/ml PPE were determined by agar diffusion method and the results were compared with those obtained using 200 mg/ml non-encapsulated PPE. Data showed that nano-encapsulated PPE has larger inhibition zone against tested bacteria after 48 h incubation (p < 0.05). As a novel coating, the nanosphere containing PPE showed higher microbial inhibitory effects in fresh chicken breast meat during the storage time (at 4 °C for two weeks), compared with those obtained using alginate containing PPE and free PPE. It seems, because of controlled release of PPE through nanospheres, a higher antimicrobial activity was achieved, so it had the potential to use as an effective antimicrobial agent in food products.

Incidence of Staphylococcus aureus in chicken meat in Aswan city,Egypt

Poultry meat is highly palatable, digestible, desirable and nutritious for all ages in addition to low price in comparison to beef meat. Therefore a total of 100 Fresh and frozen thigh and breast chicken meat samples were collected from Aswan city supermarkets, abattoirs, butcher's shops for evaluation of the incidence of contamination with Staph. aureus. The results revealed that the incidence of isolated Staph. aureus from frozen chicken thigh, breast ,fresh chicken thigh and breast were 40, 36, 32 and 32%; This result at frozen chicken meat more than fresh chicken meat may be due to excess handling of frozen meat and leave to more time at surfaces with un clean utensils before freezing. While Staphylococcal count in the same examined samples were7.55x102, 5.8x102, 4.11x103, 2.53x102; respectively. The results were reversed and therefore modified by confirmation of the results by using Polymerase Chain Reaction (PCR), revealed that fresh chicken thigh samples were positive for sea gene of Staph-aureus strains, frozen chicken meat samples were positive for sec gene of Staph- aureus and fresh chicken thigh, frozen chicken breast samples were negative for sea, seb, sed and sea genes of Staph. aureus.

Active packaging based on PLA and chitosan-caseinate enriched rosemary essential oil coating for fresh minced chicken breast application

Active packaging systems are considered an effective way to prolong the shelf life of fresh food products. This study compared five different biopolymer films for their ability to delay the lipid oxidation of raw chicken meat. New antioxidant poly lactic acid film was prepared by coating the film surface with chitosan or chitosan/caseinate blend enriched with rosemary essential oil at concentration of 1% and 2%. Films were characterized in terms of microstructure, water vapor permeability, mechanical properties, and antioxidant capacity. In vivo study was done using fresh minced chicken meats stored at 4 °C and analysed after 0, 4, 7, 11, 14, 21 days. Results indicated that water vapor transmission rate was reduced by the presence of the coating and assumed the lowest value (1.23 ± 0.03 × 10−4 g m-2 s-1) with coating enriched with 2% of rosemary essential oils. The films showed an antioxidant capacity of maximum 6% equivalent to the antioxidant capacity of 6,25 μg of REO/mL. Results from in vivo test showed that the active films were able to reduce meat oxidation during storage in anaerobic modified atmosphere condition: samples packed with active film showed constant malondialdehyde (MDA) and colour up to 14 days and reduction of heptanal and ethanol concentration compared to samples packed with control film (72 % and 90 % respectively). Overall, this study has demonstrated that PLA films coated with an active coating are a promising delivery method for providing antioxidant effects in packaging for fresh meat products.