Fresh Beef Cattle Manure Research Articles

Solid beef cattle manure application impacts on soil properties and 17β-estradiol fate in a clay loam soil

Livestock manure applied to agricultural land is one of the ways natural steroid estrogens enter soils. To examine the impact of long-term solid beef cattle (Bos Taurus) manure on soil properties and 17β-estradiol sorption and mineralization, this study utilized a soil that had received beef cattle manure over 35 years. The 17β-estradiol was strongly sorbed and sorption significantly increased (P < 0.05) with increasing soil organic carbon content (SOC) and with an increasing annual rate of beef cattle manure. The 17β-estradiol mineralization half-life was significantly negatively correlated, and the total amount of 17β-estradiol mineralized at 90 days (MAX) was significantly positively correlated with 17β-estradiol sorption. The long-term rate of manure application had no significant effect on MAX, but the addition of fresh beef cattle manure in the laboratory resulted in significantly (P < 0.05) smaller MAX values. None of the treatments showed MAX values exceeding one-third of the 17β-estradiol applied.

Survival of Escherichia coli in Beef Cattle Fecal Pats Under Different Levels of Solar Exposure

Understanding the survival and transport of Escherichia coli in feces on land and in water is important when trying to assess contamination of water by grazing animals. A fecal-pat experiment was conducted in July and August of 2003 to investigate the survival of E. coli under 4 levels of solar exposure controlled by using shade cloth. Fresh beef cattle manure was uniformly blended to produce 2.5- and 1.6-kg fecal pats, which were placed in plastic trays or in contact with the soil and covered with 0%, 40%, 80%, or 100% shade cloth treatments and replicated 5 times. Samples from each fecal pat were collected at Time 0 to establish E. coli levels; sampling was repeated at Day 1, Day 3, and approximately weekly thereafter for 45 days to determine die-off. E. coli concentration and percent moisture were measured for each fecal sample. At the end of the experiment, fecal pats under the 0% shade cloth had the lowest E. coli concentrations, followed by the 40%, 80%, and 100% treatments, with 0.018, 0.040, 0.11, and 0.44 × 106 colony-forming units (CFU) · g−1, respectively. Fecal-pat size was significant only on Day 17, when large fecal pats had higher concentrations of E. coli (P < .0001). There was no significant difference (P = 0.43) in E. coli concentration between the fecal pats in contact with the soil vs. those in plastic trays. Percent moisture of fecal pats was not a good covariate. Age of fecal pats, as well as exposure to solar radiation negatively influences the survival of E. coli. From a management perspective, E. coli in fecal pats under forested situations would survive longer than in open grasslands due to shading, and any possible contamination by E. coli would be greatest within 7 days of removing cattle from a riparian area or pasture.

Survival of Escherichia coli in Beef Cattle Fecal Pats Under Different Levels of Solar Exposure

Understanding the survival and transport of Escherichia coli in feces on land and in water is important when trying to assess contamination of water by grazing animals. A fecal-pat experiment was conducted in July and August of 2003 to investigate the survival of E. coli under 4 levels of solar exposure controlled by using shade cloth. Fresh beef cattle manure was uniformly blended to produce 2.5- and 1.6-kg fecal pats, which were placed in plastic trays or in contact with the soil and covered with 0%, 40%, 80%, or 100% shade cloth treatments and replicated 5 times. Samples from each fecal pat were collected at Time 0 to establish E. coli levels; sampling was repeated at Day 1, Day 3, and approximately weekly thereafter for 45 days to determine die-off. E. coli concentration and percent moisture were measured for each fecal sample. At the end of the experiment, fecal pats under the 0% shade cloth had the lowest E. coli concentrations, followed by the 40%, 80%, and 100% treatments, with 0.018, 0.040, 0.11, and 0.44 X 10^6 colony-forming units (CFU) g-1, respectively. Fecal-pat size was significant only on Day 17, when large fecal pats had higher concentrations of E. coli (P &lt; .0001). There was no significant difference (P = 0.43) in E. coli concentration between the fecal pats in contact with the soil vs. those in plastic trays. Percent moisture of fecal pats was not a good covariate. Age of fecal pats, as well as exposure to solar radiation negatively influences the survival of E. coli. From a management perspective, E. coli in fecal pats under forested situations would survive longer than in open grasslands due to shading, and any possible contamination by E. coli would be greatest within 7 days of removing cattle from a riparian area or pasture.