Frequent Use Of Antibiotics Research Articles

Antimicrobial Potential of Scorpion-Venom-Derived Peptides

The frequent and irrational use of antibiotics by humans has led to the escalating rise of antimicrobial resistance (AMR) with a high rate of morbidity-mortality worldwide, which poses a challenge to the development of effective treatments. A large number of host defense peptides from different organisms have gained interest due to their broad antibacterial spectrum, rapid action, and low target resistance, implying that these natural sources might be a new alternative to antimicrobial drugs. As important effectors of prey capture, defense against other animal attacks, and competitor deterrence, scorpion venoms have been developed as important candidate sources for modern drug development. With the rapid progress of bioanalytical and high throughput sequencing techniques, more and more scorpion-venom-derived peptides, including disulfide-bridged peptides (DBPs) and non-disulfide-bridged peptides (NDBPs), have been recently identified as having massive pharmacological activities in channelopathies, pathogen infections, and cancer treatments. In this review, we summarize the molecular diversity and corresponding structural classification of scorpion venom peptides with antibacterial, antifungal, and/or antiparasitic activity. We also aim to improve the understanding of the underlying mechanisms by which scorpion-venom-derived peptides exert these antimicrobial functions, and finally highlight their key aspects and prospects for antimicrobial therapeutic or pharmaceutical application.

Factors Affecting the Use of Probiotics during Antibiotic Therapy

Aim: To indicate the importance of the use of probiotics and the factors that influence the use of probiotics during antibiotic therapy. Introduction: Antibiotics are pharmacological agents selectively toxic to bacteria, but non-toxic or acceptably toxic to humans, prescribed and used in the treatment of bacterial infections. The most common gastrointestinal complication during antibiotics use is diarrhea. Probiotics are live microorganisms, bacteria and yeasts, which are used in the therapy of numerous conditions, especially in intestinal diseases. Methods: An anonymous survey of 207 patients from Bosnia and Herzegovina who used antibiotics and probiotics in the period from February 2023 to March 2024. Results: The results indicate that the use of antibiotics is frequent. About a third of the examined patients used the probiotic occasionally or not at all during the duration of the antibiotic therapy. About two thirds of the patients took the probiotic on their own initiative. A large part of the examined patients is familiar with the purpose of using probiotics. The price of probiotics plays a significant role in the application and length of use of probiotics. Discussion: Frequent use of antibiotics can increase the likelihood of gastrointestinal side effects, especially diarrhea. The use of probiotics is often shorter than the duration of antibiotic therapy. It’s use is often occasional, and on patients initiative, mostly because of the price of probiotics. Conclusion: The obtained results indicate that it is important to point out the purpose of the use of probiotics in addition to antibiotics to patients, and that a decrease in the price of probiotics would increase the length of use of probiotics.

A-278 Prevalence of Clostridium Difficile in Long-Term Care Facilities Using BD Max Cdiff Molecular Assay

Abstract Background Clostridium difficile (C.Diff) is a gram positive anaerobe producing Enterotoxin and Cytotoxin (toxin A and B respectively); more than half of million infection is diagnosed yearly with more than 10% of the cases are in patients over 65 years old. Residents in Long-Term Care Facilities (LTCF) are more prone to have C. Diff infection (CDI) because of their age, the frequent use of antibiotics, fragilities, co-morbidity, and living environment. Diagnosing CDI is based on clinical signs and symptoms as well as laboratory tests; however, the enzyme immunoassays are reliable, the tissue culture cytotoxicity is labor intensive. The nucleic acid amplification tests are becoming more the standard as first step in the diagnosis. The BD MAX™ System is a PCR walkaway instrument that provides the sensitivity, specificity and faster turnaround for the C. diff. Methods The BD MAX™ System is an automated in-vitro diagnostic test for the direct qualitative detection of the C. difficile toxin B gene (tcdB) in human liquid or soft stool specimens from patients suspected of having Clostridium difficile Infection (CDI). Premier Toxins A & B is an enzyme immunoassay for the direct detection of Clostridium difficile toxin A and toxin B in stool samples. We collected data from 5,267 samples were collected from resident in LTCF and were run using the EIA assay, 275 samples were ran using the BD Max molecular assay. We had 31 samples that were run using both assays. Statistical analyses were done using Analyse-it. Results Female accounted for 60% of the patients tested; the positivity rate was 13.2% for female and 17.2 for male using the EIA. The positivity rate was 19.2% for female and 30.6% for male when using the molecular assay. The agreement between the EIA and the PCR was 87% based on 31 samples that were run for both assays. Conclusions The BD Max gave the benefit of a fully automated with high sensitivity and specificity for the presence of toxin B producing C. Diff. The difference between the methods could be due to the lack of the sensitivity in the EIA and the fact that the molecular assay might be picking up colonized specimen or the beginning or end of infection. However, the ability to diagnose CDI early and prevent spreading of the disease to other patients is a huge benefit to the facilities. We have to keep in mind also that C.diff infection can be prevented with appropriate use of antibiotics and implementing antibiotic stewardship and infection control plan to prevent and stop spreading of the infection.

Epidemiological investigation of allergic rhinitis in children aged 6-12 years in Bayannur City, China.

Allergic rhinitis (AR) is an inflammatory condition of the nasal mucosa triggered by exposure to non-harmful substances. Over the past decade, the prevalence of AR in Chinese children has been steadily increasing. However, detailed epidemiological data on AR in children from Bayannur City are lacking. This study randomly selected six primary schools in Bayannur City. Electronic questionnaires were distributed via the web, and parents and children completed the questionnaires by scanning the two-dimensional code within a designated timeframe. Statistical analysis was performed on the collected data. A total of 4,754 valid responses were obtained. The self-reported prevalence of AR among children in Bayannur city was 39.79%. Multivariate analysis revealed that male gender, belonging to an ethnic minority, a history of food or drug allergies, frequent antibiotic use (≥3 times per year in the past two years, with each course lasting ≥3 days), and residence in urban or pastoral areas was associated with an increased prevalence of AR in children. The proportion of children experiencing moderate to severe AR hat impacted their studies or daily life was 48.78%. Chronic AR was reported in 56.71% of cases. Among AR patients with other allergic conditions, the incidence rates were as follows: bronchial asthma 35.99%, upper airway cough syndrome (UACS) 64.32%, secretory otitis media (SOM) 22.41%, obstructive sleep apnea hypopnea-syndrome (OSAHS) 49.58%, allergic dermatitis (AD) 48.72%, and allergic conjunctivitis (AC) 85.20%. The prevalence of AR was 50.30% in urban areas, 13.733% in rural areas and 20.90% in pastoral areas. Seasonal effects on AR prevalence were notably significant in urban and pastoral regions. The prevalence of AR among children in Bayannur city was 39.80%. Of those with AR, 48.72% experienced significant impacts on their learning or daily life, while only 14.80% had no other allergic conditions. There were significant variations in the prevalence and onset of AR among children between urban, agricultural and pastoral areas.

Identification and Antimicrobial Susceptibility of Serovar Choleraesuis in a Salmonella Outbreak in Central-Western Albania

Abstract Swine paratyphoid infection is caused by the swine-adapted Salmonella enterica serovar Choleraesuis. Infected individuals remain healthy carriers, and if the infection occurs at the end of the fattening period, it could pose a threat to human health. The present work aimed to assess antimicrobial susceptibility of S. enterica serovar Choleraesuis serotypes isolated from domestic pigs in central-western Albania, and to assess the presence and circulation of antibiotic-resistant paratyphoid Salmonella in Albania. Increasing mortality was reported in 6 farrow-to-finish pig farms located in the western and central parts of the country from December 2022 to April 2023. Post-mortem examination revealed macroscopic lesions in different organs indicating Salmonella infection. Four serovars Choleraesuis were isolated out of 24 different Salmonella spp. Multi-drug resistance (MDR), extensively drug-resistant (XDR), and Pan Drug Resistance (PDR) was calculated. Three out of four isolates showed MDR, and one out of four showed XDR. All S. enterica serovar Choleraesuis isolates showed resistance to at least two antimicrobials. Three isolates were observed to display MAR index values >0.2 indicating high-risk contaminated sources with frequent use of antibiotics. The predominant antimicrobial resistance was observed for amoxicillin, oxytetracycline, sulfamethoxazole, trimethoprim, spiramycin, colistin sulpha, and doxycycline. The findings emphasize the importance of strict biosecurity measures in affected pig farms, and prevention and control of S. enterica serovar Choleraesuis in the farm sanitary programs in Albania.

A proof-of-principle study for the point-of-care detection of ESBL (CTX-M) by NG-Test® CTX-M MULTI lateral flow assay in urine samples using a simplified method for use in a resource-limited setting.

The rise of extended-spectrum β-lactamase-producing Enterobacterales (ESBL-E) in low- and middle-income countries limits treatment options, leading to the frequent use of broad-spectrum antibiotics. Reducing time-to-result for a urinary infection can facilitate correct antibiotic treatment and support antimicrobial and diagnostic stewardship measures. This study compared two simplified enrichment methods for detecting CTX-M directly from urine specimens. Two enrichment methods, namely centrifugation of 2 mL urine and filtration of 1 mL urine using the DirecTool adaptor, were compared using 20 culture-positive urine samples (20 suspected ESBL-E and 20 non-ESBL-E). CTX-M production was detected using a lateral flow assay (LFA), NG-Test® CTX-MMULTI. The presence of bla CTX-M genes was confirmed by whole-genome sequencing (WGS). The results of both enrichment methods were identical, with a sensitivity of 87.5% and a specificity of 100%. In 19/20 (95%) of the urine samples, the results of the CTX-M LFA were identical with the phenotypic confirmation and WGS. Both methods could detect ESBL-E bacteriuria with ≥104 cfu/mL. All ESBL-E-negative samples were identified accurately. Both enrichment methods yielded negative results in one ESBL-E-positive (CTX-M-15) sample despite phenotypic and genotypic confirmation of ESBL production. High leukocyte count (>500 cells/µL), the presence of boric acid or polymicrobial samples did not appear to impact the performance of both enrichment methods. Our study underscores the feasibility of directly detecting CTX-M in urine. Simplified enrichment methods, particularly with a filtration kit, enhance the assay's practicality, rendering it suitable for use in primary care, emergency departments or remote laboratories without sophisticated equipment.

Recurrent urinary tract infections and prophylactic antibiotic use in women: a cross-sectional study in primary care.

Despite the considerable morbidity caused by recurrent urinary tract infections (rUTIs), and the wider personal and public health implications from frequent antibiotic use, few studies adequately describe the prevalence and characteristics of women with rUTIs or those who use prophylactic antibiotics. To describe the prevalence, characteristics, and urine profiles of women with rUTIs with and without prophylactic antibiotic use in Welsh primary care. This was a retrospective cross-sectional study in Welsh general practice using the Secure Anonymised Information Linkage (SAIL) Databank. The characteristics of women aged ≥18 years with rUTIs or using prophylactic antibiotics from 2010 to 2020, and associated urine culture results from 2015 to 2020, are described. In total, 6.0% (n = 92 213/N = 1 547 919) had rUTIs, and 1.7% (n = 26 862/N = 1 547 919) were prescribed prophylactic antibiotics with the rates increasing after 57 years of age. Only 49.0% (n =13 149/N = 26 862) of users of prophylactic antibiotics met the definition of rUTIs before initiation. The study found that 80.8% (n = 44 947/N = 55 652) of women with rUTIs had a urine culture result in the preceding 12 months with high rates of resistance to trimethoprim and amoxicillin. Of women taking prophylactic antibiotics, 64.2% (n = 9926/N = 15 455) had a urine culture result before initiation and 18.5% (n = 320/N = 1730) of women prescribed trimethoprim had resistance to it on the antecedent sample. A substantial proportion of women had rUTIs or incident prophylactic antibiotic use. However, 64.2% (n = 9926/N = 15 455) of women had urine cultured before starting prophylaxis. There was a high proportion of cultured bacteria resistant to two antibiotics used for rUTI prevention and evidence of resistance to the prescribed antibiotic. More frequent urine cultures for rUTI diagnosis and before prophylactic antibiotic initiation could better inform antibiotic choices.

Ready-to-use diagnostic kit based on electrochemical nanobiosensor for antibiotic resistance gene determination

Nowadays, antibiotic resistance, which occurs as a result of mutations in bacterial genes due to the frequent use of antibiotics, causes serious health problems and deaths. One of the resistance mechanisms that develop with the use of antibiotics is that bacteria begin to produce carbapenemase enzymes that cause multiple drug resistance in bacteria by hydrolyzing antibiotics. In this study, an alternative diagnostic kit method to traditional tests was developed for the determination of bacteria carrying relevant antibiotic resistance genes because these analyzes have multi-step sample preparation, long/complex determination schemes and are high cost.With the diagnostic kit containing a stable electrochemical nanobiosensor developed in this study, the presence of two different antibiotic resistance gene regions of the carbapenemase enzyme named OXA-48 and VIM, which are responsible for more than 50 % of deaths due to antibiotic resistance, was investigated. Carbon nanotubes (CNTs) has been modified to the pencil graphite electrode (PGE), then the synthetic capture probe DNAs are immobilized covalently to the surface containing nanomaterial. Later, the sensor surface, whose stability was ensured by surface blockage, thus gained “diagnostic kit feature” and was stored in the refrigerator (+4 °C) until analysis. Thus, the preparation of a ready-to-analyze diagnostic kit, which will provide detection without any preparation, has been completed. With the developed kit, it was possible to determine the resistance gene with 2.5 pmole/50 μL of detection limit. In addition, analyzes with real samples could be performed even after 150 days (5 months) of kit preparation.This represents the first diagnostic kit system containing PGE and CNT for the detection of antibiotic resistance based on electrochemical alpha naphthol signal measurement in real samples with symmetrical and asymmetric structures (PCR). In the developed alternative test platform, the sample volume required for determination is 50 μL and the analysis time with this portable open-use and throw-away diagnostic kit is 30 min.

A case-control epidemiological survey on potential risk factors for celiac disease.

Celiac disease (CD) is a chronic autoimmune disorder caused by a complex interplay between genetic and environmental factors. The main goal of our case-control study was to analyse the association of environmental factors with the odds of CD development in a sample of the Slovak population. Data were collected from 1,226 respondents (534 CD patients and 692 controls) by a questionnaire. The impact of analysed parameters on the chance of disease development was assessed by multiple regression analysis and expressed as odds ratios (OR). Values of p < 0.05 were considered statistically significant. In the patient group, celiac disease was significantly more prevalent in women than in men (OR = 1.52, p = 0.010). Respondents with a positive family history of CD showed 2.9-fold higher odds of CD compared to others (p < 0.001), and respondents with coexisting autoimmune diseases had 2.6-fold higher odds of CD (p < 0.001). Subjects who had taken antibiotics at least three times a year during childhood had 1.95-fold higher odds of developing CD compared to those who took them less frequently or not at all (p = 0.022). Conversely, individuals who were breastfed in infancy had lower odds of CD compared to non-breastfed respondents (OR = 0.53, p < 0.001). The mode of delivery (vaginal vs. caesarean section), overcoming severe infections, and the timing of gluten introduction in childhood did not show a statistically significant effect on the odds of developing CD. Based on our data, being female, having a positive family history of CD, suffering from another autoimmune disease, and frequent use of antibiotics are factors associated with an increased chance of developing CD. On the other hand, breastfeeding in infancy seems to have a protective effect. Our findings highlight the importance of further research in understanding the complexities of this autoimmune condition and providing a foundation for prevention strategies.

The CRISPR/Cas system as an antimicrobial resistance strategy in aquatic ecosystems.

With the growing population, demand for food has dramatically increased, and fisheries, including aquaculture, are expected to play an essential role in sustaining demand with adequate quantities of protein and essential vitamin supplements, employment generation, and GDP growth. Unfortunately, the incidence of emerging/re-emerging AMR pathogens annually occurs because of anthropogenic activities and the frequent use of antibiotics in aquaculture. These AMR pathogens include the WHO's top 6 prioritized ESKAPE pathogens (nosocomial pathogens: Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp.), extended-spectrum beta lactases (ESBLs) and carbapenemase-producing E. coli, which pose major challenges to the biomagnification of both nonnative and native antibiotic-resistant bacteria in capture and cultured fishes. Although implementing the rational use of antibiotics represents a promising mitigation measure, this approach is practically impossible due to the lack of awareness among farmers about the interplay between antimicrobial use and the emergence of antimicrobial resistance (AMR). Nevertheless, to eradicate these 'superbugs,' CRISPR/Cas (clustered regularly interspersed short palindromic repeats/CRISPR associate protein) has turned out to be a novel approach owing to its ability to perform precise site-directed targeting/knockdown/reversal of specific antimicrobial resistance genes in vitro and to distinguish AMR-resistant bacteria from a plethora of commensal aquatic bacteria. Along with highlighting the importance of virulent multidrug resistance genes in bacteria, this article aims to provide a holistic picture of CRISPR/Cas9-mediated genome editing for combating antimicrobial-resistant bacteria isolated from various aquaculture and marine systems, as well as insights into different types of CRISPR/Cas systems, delivery methods, and challenges associated with developing CRISPR/Cas9 antimicrobial agents.

Associations between Polycystic Ovary Syndrome (PCOS) and Antibiotic Use: Results from the UAEHFS.

Women with polycystic ovary syndrome (PCOS) have a higher susceptibility to infections compared to those without PCOS. Studies evaluating antibiotic use based on PCOS status are scarce. Therefore, we aimed to (i) assess the associations between self-reported PCOS and antibiotic use, and (ii) whether PCOS treatment and the age at PCOS diagnosis modified the associations above. This cross-sectional analysis used the United Arab Emirates Healthy Future Study (UAEHFS) conducted from February 2016 to March 2023 involving 2063 Emirati women aged 18-62 years. We performed ordinal logistic regressions under unadjusted and demographic-health-characteristic-adjusted models to obtain the odds ratios (ORs) and 95% confidence intervals (CIs) to analyze PCOS and antibiotic use. Subgroup analyses were performed by treatment status and age at diagnosis. We found that women with PCOS were 55% more likely to frequently take a course of antibiotics in the past year (aOR 1.55; 95% CI 1.26-1.90). Similar likelihoods were also found among those being treated for PCOS and those without treatment but with a PCOS diagnosis at ≤25 years. Our study suggests that PCOS was associated with an increased use of antibiotics among Emirati women. Understanding the frequent antibiotic use susceptibility among those with PCOS may improve antibiotic use surveillance and promote antibiotic stewardship in these at-risk individuals.

An adaptive drug-releasing contact lens for personalized treatment of ocular infections and injuries

This research introduces an innovative solution to address the challenges of bacterial keratitis and alkali burns. Current treatments for bacterial keratitis and alkali burns rely on the frequent use of antibiotics and anti-inflammatory eye drops. However, these approaches suffer from poor bioavailability and fluctuating concentrations, leading to limited efficacy and potential drug resistance. Our approach presents an adaptive drug-releasing contact lens responsive to reactive oxygen species (ROS) at ocular inflammation sites, synchronously releasing Levofloxacin and Diclofenac. During storage, minimal drug release occurred, but over 7 days of wear, the lens maintained a continuous, customizable drug release rate based on disease severity. This contact lens had strong antibacterial activity and biofilm prevention, effectively treating bacterial keratitis. When combined with autologous serum, this hydrophilic, flexible lens aids corneal epithelial regeneration, reducing irritation and promoting healing. In summary, this ROS-responsive drug-releasing contact lens combines antibacterial and anti-inflammatory effects, offering a promising solution for bacterial keratitis and alkali burns.

High antibiotic resistance rates in Helicobacter pylori strains in Turkey over 20 years: implications for gastric disease treatment.

Helicobacter pylori (Hp) eradication therapy is crucial for preventing the development of gastritis, peptic ulcers, and gastric cancer. An increase in resistance against antibiotics used in the eradication of Hp is remarkable. This meta-analysis aims to examine the resistance rates of Hp strains isolated in Turkey over the last 20 years against clarithromycin (CLR), metronidazole (MTZ), levofloxacin (LVX), tetracycline (TET), and amoxicillin (AMX) antibiotics. Literature search was carried out in electronic databases, by searching articles published in Turkish and English with the keywords ' helicobacter pylori ' or 'Hp' and 'antibiotic resistance' and 'Turkey'. That meta-analysis was carried out using random-effect model. First, the 20-year period data between 2002 and 2021 in Turkey were planned to be analyzed. As a second stage, the period between 2002 and 2011 was classified as Group 1, and the period between 2012 and 2021 as Group 2 for analysis, with the objective of revealing the 10-year temporal variation in antibiotic resistance rates. In gastric biopsy specimens, 34 data from 29 studies were included in the analysis. Between 2002-2021, CLR resistance rate was 30.9% (95% CI: 25.9-36.2) in 2615 Hp strains. Specifically, in Group 1, the CLR resistance rate was 31% in 1912 strains, and in Group 2, it was 30.7% in 703 strains. The MTZ resistance rate was found to be 31.9% (95% CI: 19.8-45.4) in 789 strains, with rates of 21.5% in Group 1 and 46.6% in Group 2. The overall LVX resistance rate was 25.6%, with rates of 26.9% in Group 1 and 24.8% in Group 2. The 20-year TET resistance rate was 0.8%, with 1.50% in Group 1 and 0.2% in Group 2. The overall AMX resistance rate was 2.9%, 3.8% between 2002-2011, and 1.4% between 2012-2021. Hp strains in Turkey exhibit high resistance rates due to frequent use of CLR, MTZ, and LVX antibiotics. However, a significant decrease has been observed in TET and AMX resistance to Hp in the last 10 years. Considering the CLR resistance rate surpasses 20%, we suggest reconsidering the use of conventional triple drug therapy as a first-line treatment. Instead, we recommend bismuth-containing quadruple therapy or sequential therapies (without bismuth) for first-line treatment, given the lower rates of TET and AMX resistance. Regimens containing a combination of AMX, CLR, and MTZ should be given priority in second-line therapy. Finally, in centers offering culture and antibiogram opportunities, regulating the Hp eradication treatment based on the antibiogram results is obviously more appropriate.

STUDY OF SENSITIVITY TO ANTIBACTERIAL DRUGS OF SIGNIFICANT PATHOGENS OF BRONCHOPULMONARY DISEASES IN CHILDREN

The spread of infectious diseases has significant economic consequences, as they lead to high treatment costs, reduced labor productivity and the need to implementmeasures to prevent and control epidemics. The problem of antibiotic resistance is largely related to the frequent and improper use of antibiotics in the treatment of children. The increased sensitivity of children's organisms to infections and, in some cases, the lack of alternative treatment methods lead to the spread of resistant strains of microorganisms. It is important to strictly control the use of antibiotics in children, to carry out rational and precisely dosed therapy, as well as to provide information to parents about the need to comply with prescribed prescriptions in order to reduce the risk of developing resistance and maintain the effectiveness of antibiotics in the treatment of childhood infections.

Using Quantitative Polymerase Chain Reaction (qPCR) to Identify a Myriad of Carbapenemase Genes in Fresh Cow Dung in Bangladesh.

Introduction The emergence of antimicrobial resistance (AMR) is driven by the selection pressure of frequent uses of antimicrobial agents in healthcare, the food chain, agriculture, fishery, and the food animal industry, which poses a serious health risk for transmission-linked humans and the surrounding environment. Livestock, particularly cattle, play an essential role in the food sector in Bangladesh. The food-animal chains can be the potential routes of exposure to AMR-microorganisms for every domain of one health. Antimicrobial resistance genes (ARGs) can impart a reservoir of AMR within the food supply chain, even without pathogenic microorganisms. This study investigated the history of infection for the last six-month period of antimicrobials utilized in cattle farms and the distribution of selected carbapenemase resistance genes, namely, bla-KPC, bla-IMP, bla-VIM, bla-NDM-1, bla-SIM, bla-GIM, bla-SPM, andbla-SME, in cattle feces in Bangladesh. Methods A cross-sectional study was designed to analyze ARGs in fresh cow dung samples collected from commercial farms and individual houses in four Bangladesh districts, namely, Dhaka, Gazipur, Manikganj, and Tangail. Types of cattle breeds, their existing diseases, recent antimicrobial uses, and vaccine uses were recorded. DNA was extracted from each cow dung sample using commercial kits (Qiagen GmbH, Germany). Real-time quantitative polymerase chain reaction (RT-qPCR) was employed to assess the eight carbapenem resistance genes in the extracted DNA.The eight carbapenem resistance genes in the extracted DNA were assessed byRT-qPCR using the qTOWER3 thermal cycler (Analytik Jena GmbH, Konrad-Zuse-Straße 1, 07745 Jena, Germany). Results Group A carbapenemase, bla-KPC, was detected in 66.7% of the samples. However, no bla-SME was identified in all of the test samples. Group B metallo carbapenemase, bla-IMP, bla-NDM-1, bla-VIM, bla-SIM, bla-GIM, andbla-SPM, were in 66.7% (80/120), 49.2% (59/120), 48.3% (58/120), 68.3% (82/120), 58.3% (70/120), and 12.5% (15/120), respectively. Only 8.3% of the tested samples contained no MBL gene; 10% carried a single-type carbapenemase gene; and the remaining 81.7% carried two or more carbapenemase genes concurrently. Co-carriage of four or more genes was found in over 59% of samples. As many as seven genes were found together in 6.7% of samples. ARG detection in commercial cattle samples and household feces is not statistically significant. Conclusions Substantial carbapenem-resistance ARGs were detected in commercially farmed cow dung and household cattle samples. Frequent use of antibiotics for cattle for treatment and prophylactic purposes may influence the high acquisition of ARGs. Bangladeshi cattle farms are reservoirs and routes of AMR, posing a significant threat to the country's public health.

Detection of Tetracycline Resistance Genes and Susceptibility Profile of Bacteria Isolated from Clinical Samples

The use of Antibiotics by humans for an extensive period of time has an impact not just on the disease being treated, but also on the microbial population in that environment. Antibiotic abuse in many developing nations have aided the emergence and widespread transmission of antibiotic resistant resistance genes in the environment. Tetracycline is a broad-spectrum antibiotic used to treat diseases in both humans and animals. Tetracycline is regulated by tet genes, which are engaged in active drug efflux, ribosome protection, or enzymatic drug modification. The use of Tetracycline’s as a treatment option for treating oral and systemic disorders haves been limited due to the existence of these genes. Hence, this study aimed to detect tetracycline tet (A) and tet (B) resistance genes in bacterial isolated from clinical samples and to determine their susceptibility profile. An experimental design was used for this study. A total of forty human clinical isolates from blood, urine, throat, wound, and sputum were randomly selected for this study. The isolates were collected and sub-cultured in nutrient broth for analysis and PCR was used for the detection of genes. The result showed that antibiotics resistant genes were present in the all of the isolates. However, tetB had the highest occurrence (91.6%) of than tetA (8.4%). All the isolates showed high resistant to the various antibiotics including ceftazidime, cefuroxime, gentamicin, ofloxacin, amoxicillin, nitrofurantoin, cefixime, and ciprofloxacin. This could be due to the frequent use of broad-spectrum antibiotics in clinical settings which have led to the development of antibiotic resistant strains among bacteria. Antibiotics resistant genes (tetA &amp; tetB) are present in bacteria isolated from clinical setting and may become a serious public health concern when these genes are transferred among organisms that affect both humans and animals. Hence, preventative measures should be employed to reduce the diffusion of these genes in the clinical setting since they present a serious public health concern when they are transferred among organisms that affect both humans and animals.

The “Carpenter,” a substrate for green synthesis: Biosynthesis and antimicrobial potential

The frequent use of antibiotics has created favorable conditions for bacteria to develop resistance. Bacterial resistance is an issue for global health killing at least 1 million people worldwide annually. The quest for new and effective antimicrobials with activities against resistance bacteria demands immediate attention. The use of nanoparticles as antimicrobials alternatives to conventional antibiotics may have the potential to combat bacterial resistance. Silver nanoparticles, silver nanoparticles have captivated the interest of most researchers by virtue of their broad-range antimicrobial activity against bacteria due to their strong biocidal effect against microorganisms. Silver nanoparticles have been traditionally synthesized by physical chemical and biological process. However, biosynthesis of silver nanoparticles from wings of carpenter bees Xylocopa virginica, which is abundantly available in summer in the United States of America, is yet to be explored. Herein, we report the synthesis of silver nanoparticles using wing extract of carpenter bees X. virginica. Subsequently the biosynthesized nanoparticles were characterized by UV&amp;ndash;Visible absorption spectroscopy and scanning electron microscopy (SEM). Furthermore, the antimicrobial activity of the biosynthesized nanoparticles was investigated against two common Gram-negative and Gram-positive pathogenic bacteria viz Klebsiella pneumonia, Escherichia coli, Micrococcus luteus and Staphylococcus aureus using microdilution method. The results of this study indicate that biosynthesized silver nanoparticles from X. virginica wing extract showed an absorption band at 440 nm and SEM revealed spherical nanoparticles 20- 60 nm in size. Additionally, biosynthesizes silver nanoparticles from the wings of X. virginica exhibited antimicrobial activity against all the tested bacteria signifying their biomedical, pharmaceutical, and agricultural potential.

Genotypic Profiling of Virulence and Antibiotic Resistance Patterns of Staphylococci and Streptococci Isolates from Dairy Cattle with Clinical Mastitis Infection in Region IV-A, Philippines

Staphylococci and Streptococci spp. play a significant role as primary causal agents of bovine mastitis. Farmers commonly employ the use of antibiotics as a widespread strategy for the management of mastitis infections. However, the frequent use of antibiotics is directly correlated to the increase in antimicrobial-resistant (AMR) bacterial strains resulting in some challenges in managing subsequent infections. In this current study, the virulence and AMR profiles of previously isolated Staphylococci and Streptococci bacterial strains from dairy cattle with clinical mastitis were evaluated to provide an overview of their pathogenic and resistant genetic characteristics. The incidence of virulence genes among Staphylococci isolates revealed low detection rates, whereas 42.2% of the Streptococci isolates were found to express the surface-anchored rib gene. The laminin-binding lmb and toxin β-hemolysin cylE genes were also frequently detected among Streptococci isolates. These observations suggest a strong association between Streptococci spp. and severe infection among the tested animals. Meanwhile, 28% of Staphylococci and 12.2% of Streptococci were observed to be resistant to three or more drug classes, thus considered multidrug-resistant. The most frequently detected AMR genes among Staphylococci isolates were aphA-1 (33%), mecA (25%), and blaSPM-1 (21%). Meanwhile, among Streptococci isolates, tetB (35%), blaIMP (31%) and ermA/TR (31%) were most prevalent. Although the phenotypic resistance of some isolates was comparable with its associated genotypic resistance, some isolates did not exhibit a consistent phenotype-genotype AMR. Nevertheless, the presence of these virulence and AMR genes in mastitis-associated pathogens is still critical because these genes can migrate across microbial populations, thus posing a serious threat to animal and public health.

Impact and Diversity of ESBL-Producing Klebsiella pneumoniae Recovered from Raw Chicken Meat Samples in Türkiye.

The interrelationship between human, animal and environmental sectors leads to the spread of antibiotic resistance due to selective pressures, evolutionary traits and genomic evolution. In particular, the frequent use of antibiotics in livestock inevitably influences the emergence of specific resistance determinants in human strains, associated with reduced treatment options in clinical therapy. In this study, ESBL-producing Klebsiella pneumoniae strains isolated from chicken meat samples were evaluated for public health implications in Türkiye. Whole-genome sequencing was used for genetic dissection and phylogenetic comparison of their genomes. The isolates were assigned to four MLST types (ST147, ST37, ST2747 and ST219); two of them were found to represent the ST147 clone associated with severe human infections worldwide. In addition to cephalosporins, high resistance levels to quinolones/fluoroquinolones were identified phenotypically, caused by acquired resistance genes and chromosomal point variations. One isolate was also found to carry the qacE∆1 efflux transporter gene, which confers tolerance to quaternary ammonium compounds. The detection of virulence genes (i.e., that coding for enterobactin) associated with the pathogenicity of K. pneumoniae suggests a public health impact. Thus, comprehensive information on the occurrence and impact of K. pneumoniae from livestock is needed to derive appropriate management strategies for consumer protection. In this study, it was shown that poultry meat serves as a reservoir of clinically emerging multidrug-resistant high-risk clones.

Deployment of in-silico analysis to reveal the antibacterial profiles of Allium sativum against Aeromonas hydrophila

The key challenges in aquaculture are the emergence of antimicrobial resistance in fish cultivation due to the frequent use of antibiotics. Over the past three decades, this led to a major threat in the persistence of multidrug-resistant bacteria. Aeromonas hydrophila is a Gram-negative bacterium, a common causative agent of motile bacterial septicemia in fisheries. Combining these two key factors of the presented narrative, the essential type II topoisomerase enzyme ‘DNA gyrase’ (encoded by the gyrA and gyrB genes) as a potential drug target in Aeromonas hydrophila was taken, retrieve its sequence from UniProtKB (Id-A0KKQ2), constructs the 3-D structure using SWISS-MODEL (in absence of the experimental structure), and performs an in-silico screening of selected drug-like compounds (25 antibacterial phytochemicals) most of which are bioactive compounds of A. sativum through molecular docking. Quercetin a derivative of A. sativum was observed as a more potent drug molecule than other studied molecules based on ligand binding energy as docking score −7.812, showed highly encouraging results, supported by a study using structural dynamics of the receptor-ligand complex for a duration of 100 ns by Molecular Dynamic Simulations and confirm binding stability with MM-GBSA calculations. This study also provides theoretical grounds for drug discovery against other pathogenic bacteria posing threats to the ecosystem. Switching to herbal products is the best way to combat the plurality of problems to avoid seen or unseen post-treatment side effects. Communicated by Ramaswamy H. Sarma