Frequency Electromagnetic Radiation Research Articles

Quantitative assessment of the results of lymphovenous edema therapy in patients after combined treatment of breast cancer

Background. Secondary lymphovenous edema of the hand is one of the most common and serious complications of specialized treatment of breast cancer. Purpose – to quantify the dynamics of treatment of lymphovenous edema of the hand by measuring its volume using the proposed device model. Materials and methods. In 23 patients, the type of radiation injury was determined by the radionuclide method, the degree of secondary hand edema was determined using a number of designed devices. Treatment was performed by irradiating the areas of radiation fibrosis and the corresponding lymphatic collectors with ultra-low frequency electromagnetic radiation (UHF EMR) and compression lymphatic drainage. Results and discussion. The dynamics of the treatment was evaluated by the change in the volume of the hand, which was determined by the amount of fluid displaced from the model of the measuring device when the distal part of the upper limb was immersed in it. Conclusions. This technique can be considered as an objective method for quantifying lymphovenous edema of the hand.

Radio frequency electromagnetic radiations interfere with the Leydig cell functions in-vitro.

A growing threat to male infertility has become a major concern for the human population due to the advent of modern technologies as a source of radiofrequency radiation (RFR). Since these technologies have become an integral part of our daily lives, thus, it becomes necessary to know the impression of such radiations on human health. In view of this, the current study aims to focus on the biological effects of radiofrequency electromagnetic radiations on mouse Leydig cell line (TM3) in a time-dependent manner. TM3 cells were exposed to RFR emitted from 4G cell phone and also exposed to a particular frequency of 1800 MHz and 2450 MHz from RFR exposure system. The cells were then evaluated for different parameters such as cell viability, cell proliferation, testosterone production, and ROS generation. A considerable reduction in the testosterone levels and proliferation rate of TM3 cells were observed at 120 min of exposure as compared to the control group in all exposure settings. Conversely, the intracellular ROS levels showed a significant rise at 60, 90 and 120 min of exposure in both mobile phone and 2450 MHz exposure groups. However, RFR treatment for different time durations (15, 30, 45, 60, 90, and 120 min) did not have significant effect on cell viability at any of the exposure condition (2450 MHz, 1800 MHz, and mobile phone radiation). Therefore, our findings concluded with the negative impact of radiofrequency electromagnetic radiations on Leydig cell's physiological functions, which could be a serious concern for male infertility. However, additional studies are required to determine the specific mechanism of RFR action as well as its long-term consequences.

Effects of non-ionizing radio frequency electromagnetic radiation on the development and behavior of early embryos of Danio rerio

ABSTRACT Biological effects of radio frequency electromagnetic radiation (RF-EMR) in the range of 900–1800 MHz emerging from the mobile phone were investigated and were found to influence the locomotor pattern when exposure was initiated from 1 hour post fertilization (hpf) in zebrafish embryos (ZE), Danio rerio. Mobile phones and other wireless devices offer tremendous advantages. However, on the flipside they are leading to an increased electromagnetic energy in the environment, an excess of which could be termed as electromagnetic pollution. Herein, we tried to understand the effects of RF-EMR emerging from the mobile phone, on the development and behavior of ZE, exposed to RF-EMR (specific absorption rate of 1.13 W/kg and 1800 MHz frequency) 1 hr daily, for 5 days. To understand if there could be any developmental stage-specific vulnerability to RF-EMR, the exposure was initiated at three different time points: 1hpf, 6hpf and 24hpf of ZE development. Observations revealed no significant changes in the survival rate, morphology, oxidative stress or cortisol levels. However, statistically significant variations were observed in the batch where exposure started at 1hpf, with respect to locomotion patterns (distance travelled: 659.1 ± 173.1 mm Vs 963.5 ± 200.4 mm), which could be correlated to anxiety-like behavior; along with a corresponding increase in yolk consumption (yolk sac area: 0.251 ± 0.019 mm2 Vs 0.225 ± 0.018 mm2). Therefore, we conclude that RF-EMR exposure influences the organism maximally during the earliest stage of development, and we also believe that an increase in the time of exposure (corresponding to the patterns of current usage of mobile phones) might reveal added afflictions.

Earthquakes Three-Stage Early Warning and Short-Term Prediction

Among the anomalous geophysical phenomena observed preceding earthquakes, specific attention has been given to VLF/LF, ULF (very low frequency/low frequency, ultra-low frequency) electromagnetic (EM) emissions, recorded before earthquakes, because of an interesting correlation between them and seismic activity. Numerous scientific papers have been published on this topic, providing convincing evidence of these processes observed using ground-based and satellite ground-based observations during the preparation for the earthquake. These phenomena are detectable both at laboratory and geological scales. The authors of this paper have used an avalanche-like unstable geological model of fault formation and a model of the generation of electromagnetic emissions detected before the earthquakes to prove the prediction capabilities of VLF/LF EM emissions. The first gives a clear imagination of earthquake preparation in all stages in the focal area from the beginning of the micro-cracks appearing to the final formation of fault. Another one, based on electrodynamics, explains the EM emissions origination process and offers a formula that analytically connects the observed frequency of EM radiation with the linear size of the emitted body (fault). It is worth emphasizing the synthesis and rather harmonious relation of the mentioned models. Based on the above models conducted studies clearly show that the description of a qualitative avalanche-unstable geological model of fault formation using VLF/LF EM radiation data made it possible to quantitatively characterize the full cycle of preparation and occurrence of earthquake process. Namely, in the case of VLF/LF emissions monitoring, the beginning of the “avalanche process” of ruptures is considered the first stage of early warning of an incoming earthquake. The so-called EM emissions “silence” period is assessed as the second stage of early warning. The third early warning or an alarm about an incoming earthquake may be announced immediately at the moment of the first anomaly appearance subsequently the “silence” period. This article offers the method of earthquake three-step early warning and short-term (hourly) prediction.

From the experience of the technical examination of hydro-technical tunnels

A comprehensive methodology is proposed and the results of the technical examination of hydraulic tunnels are given. The technique takes into account the influence of water flow, the formation of cavities behind the shell, fixes defects, and reveals the prerequisites for their formation. It consists of visual inspection and non-destructive testing (ultrasonic, electromagnetic, vibro-acoustic). A unified presentation of the examination results is proposed. The optimal basis and operating frequency of the ultrasonic method are substantiated. The gradations of the processes of the formation of cracks in concrete are determined by the frequency of electromagnetic radiation. Examples of tunnel examination are given. Characteristic areas of the objects are identified. For main canals, this is a shell near the entrance and exit portals. Cavities behind the shell can be traced along the entire length of the tunnels. The main defect of the shells of pressure tunnels of power plants is cracks in the reinforced concrete lining. For structures of seasonal drainage, filtration in the bottom is characteristic. The examination results are used to develop projects for repair works. Some methodical regulations can be used for inspecting any underground structures.

The endocannabinoid system is involved in the anxiety-like behavior induced by dual-frequency 2.65/0.8 GHz electromagnetic radiation in mice.

As wireless communication devices gain popularity, concerns about the potential risks of environmental exposure to complex frequency electromagnetic radiation (EMR) on mental health have become a public health issue. Historically, EMR research has predominantly focused on single- frequency electromagnetic waves, neglecting the study of multi-frequency electromagnetic waves, which more accurately represent everyday life. To address these concerns, our study compared the emotional effects of single-frequency and dual-frequency EMR while exploring potential molecular mechanisms and intervention targets. Our results revealed that single-frequency EMR at 2.65 or 0.8 GHz did not induce anxiety-like behavior in mice. However, exposure to dual-frequency EMR at 2.65/0.8 GHz significantly led to anxiety-like behavior in mice. Further analysis of mouse sera revealed substantial increases in corticosterone and corticotrophin releasing hormone levels following exposure to 2.65/0.8 GHz EMR. Transcriptome sequencing indicated a significant decrease in the expression of Cnr1, encoding cannabinoid receptor 1 Type (CB1R), in the cerebral. This finding was consistently verified through western blot analysis, revealing a substantial reduction in CB1R content. Additionally, a significant decrease in the endocannabinoid 2-arachidonoylglycerol was observed in the cerebral cortex. Remarkably, administering the cannabinoid receptor agonist Win55-212-2 significantly alleviated the anxiety-like behavior, and the cannabinoid receptor antagonist AM251 effectively counteracted the anti-anxiety effects of Win55-212-2. In summary, our research confirmed that dual-frequency EMR is more likely to induce anxiety-like behavior in mice than single-frequency EMR, with implications for the hypothalamic-pituitary-adrenal axis and the endocannabinoid system. Furthermore, our findings suggest that Win55-212-2 may represent a novel avenue for researching and developing anti-EMR drugs.

Влияние электромагнитного излучения Wi-Fi (2,45 ГГц) на сперматогенез и мужскую фертильность

Wireless communication (Wi-Fi) has become the preferred way of Internet communication and connectivity, has become a daily necessity and is widely used in a wide variety of devices. Widespread use of Wi-Fi (2.45 GHz) has significantly changed human life for the better, but has led to the widespread spread of radio frequency electromagnetic radiation. Scientific evidence suggests the possible negative effects of Wi-Fi on male fertility. This review examines the main pathogenetic mechanisms of male infertility due to the influence of wireless devices: oxidative stress, DNA fragmentation and apoptosis of male gametes. The results of in vitro experimental studies proving the effect of radiofrequency electromagnetic radiation on ejaculate are presented. The results of studies using in vivo experimental models were analyzed. This allowed us to get a holistic view of the effects of Wi-Fi on the male reproductive system and spermatogenesis. The presented new scientific data on the impact of everyday Wi-Fi use on spermatogenesis in men.

Effects of high-low frequency electromagnetic radiation on vibrational resonance in Hodgkin–Huxley neuronal system

In this paper, based on the Hodgkin–Huxley (H–H) neuron model, the effects of high-low frequency (HLF) electromagnetic radiation on vibrational resonance (VR) in a single neuron is investigated. It is found that VR can be observed in a single H–H neuron model with or without considering HLF electromagnetic radiation. However, HLF electromagnetic radiation can cause changes in the structure of the resonance peaks and make the maximum region and intensity of the response amplitude smaller, which weakens the VR in the neuronal system. This is because the HLF electromagnetic radiation makes the neuronal electrical activities less regular, resulting in weaker synchronization between input low-frequency signal and output firing. In addition, the effects of the system parameters such as the HF electromagnetic radiation amplitude on the Fourier coefficients are investigated, and it is found that these parameters can also cause changes in the number of resonance peaks, resulting in vibrational mono-resonance and vibrational bi-resonances. When the frequency of the HLF electromagnetic radiation is between 0.014 and 0.041, local anti-resonance can be also observed. This has practical significance in reducing some of the unexpected vibration in aerospace engineering and mechanical fields. The HLF electromagnetic radiation plays an important role in weakening the VR of neuronal systems. Therefore, this study could provide new insights to further control the abnormal electrical activities and information transmission of neurons, which can lead to better design and development of therapeutic programs for neurological disorders and neuroplasticity therapies, and advance neuroscience.

Effects of Electromagnetic Radiation and Radio Frequency on Freshwater Calanoid and Cyclopoid Copepods

Effects of Electromagnetic Radiation and Radio Frequency on Freshwater Calanoid and Cyclopoid Copepods

The Potential Impact of a High-Frequency Telecommunication Network on Cognitive Functions: A Review

The latest cellular technology, known as 5G-NR, is intended to significantly speed up and improve the effectiveness of wireless systems. A revolution in the telecom industry has been sparked by the widespread use of and increased reliance on cellular communication technology. Moreover, 5G and B5G technologies are expected to utilize an even higher-frequency range to achieve faster data transmission and lower latency communication. Consequently, while transmitting signals across various types of equipment and infrastructure, the general public is exposed to much higher frequencies of electromagnetic radiation. The increasing need for 5G NR base stations (gNodeB) has heightened public anxiety over potential negative health impacts. This study reviews recent research on the effects of electromagnetic waves on humans, particularly focusing on how these effects influence cognitive functions. Most research to date has not found significant differences in cognitive performance due to ubiquitous mobile communications. However, current research has largely been limited to 4G technologies, and the health effects of exposure to 5G user equipment (UE) and base stations in higher-frequency bands remain unexplored. If subsequent research suggests that exposure to high-frequency wireless networks significantly impacts cognitive functions, the deployment and acceptance of these technologies may face challenges and constraints. Therefore, such investigations are crucial for determining whether next-generation technologies pose no risk to individuals.

Effects of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on glioblastoma cells (U -118 MG).

Mobile phones and Wi-Fi are the most commonly used forms of telecommunications. Initiated with the first generation, the mobile telephony is currently in its fifth generation without being screened extensively for any biological effects that it may have on humans or on animals. Some studies indicate that high frequency electromagnetic radiation emitted by mobile phone and Wi-Fi connection can have a negative effect upon human health, and can cause cancer, including brain tumour. The aim of the study was to investigate the influence of 2.4 GHz radiofrequency electromagnetic field (RF-EMF) on the proliferation and morphology of normal (human embryonic kidney cell line Hek-293) and cancer cells (glioblastoma cell line U-118 MG). The cell cultures were incubated in RF-EMF at the frequency of 2.4 GHz, with or without dielectric screen, for 24, 48 and 72h. In order to analyse the influence of the electromagnetic field on cell lines, Cytotoxicity test Cell Counting Kit-8 was performed. To protect cells against emission of the electromagnetic field, a dielectric screen was used. It was found that 2.4 GHz RF electromagnetic field exposure caused a significant decrease in viability of U-118 MG and Hek-293 cells. The impact of the electromagnetic field was strongest in the case of cancer cells, and the decrease in their survival was much greater compared to the healthy (normal) cells of the Hek-293 line. Results of the study indicate that using a radio frequency electromagnetic field (2.4 GHz) has a clearly negative effect on the metabolic activity of glioblastoma cells. RF-EMF has much less impact on reducing the viability of normal cells (Hek -293) than cancer cells.

Effects of Electromagnetic Radiation on Neuropeptide Transcript Levels in the Synganglion of Ixodes ricinus.

Anthropogenic electromagnetic radiation is an important environmental factor affecting the functionality of biological systems. Sensitivity to various frequencies of electromagnetic radiation has been detected in ixodid ticks in the past. However, the physiological aspects of radiation effects have not yet been studied in ticks. In the presented experiment, 360 Ixodes ricinus ticks, 180 males and 180 females, were divided into 16 irradiated and 8 control groups. The irradiated groups were exposed to two different intensities of electromagnetic radiation with a frequency of 900 MHz at different lengths of exposure time. RT-PCR was utilized to determine the changes in mRNA levels in tick synganglia after irradiation. Four randomly selected neuropeptide genes were tested-allatotropin (at), FGLa-related allatostatins (fgla/ast), kinin, and arginine-vasopressin-like peptide (avpl). A significant decrease in transcript levels in all female groups exposed to higher intensity radiofrequency radiation for 1 to 3 h was found. After one hour of radiofrequency exposure, a significant downregulation in allatotropin expression in males was detected. A consistent downregulation of the at gene was detected in males irradiated with at a higher intensity. Unfortunately, the specific functions of the studied neuropeptides in ticks are not known yet, so a more comprehensive study is necessary to describe the effects of EMF on observed neuropeptides. This study represents the first report on the effects of the abiotic environment on tick neurophysiology.

Interspecific differences in the behavioral response of ticks exposed to radiofrequency electromagnetic radiation.

Artificial electromagnetic radiation is a new environmental factor that affects animals. Experiments with the effect of radio frequency electromagnetic radiation were focused on both vertebrates and invertebrates. Ticks showed a significant affinity to radiation. Our study is a continuation of this research and its aim was to monitor the effect of radiation on the behavior of four tick species: Ixodes ricinus, Dermacentor reticulatus, Dermacentor marginatus and Haemaphysalis inermis. In total 1,200 ticks, 300 of each species, were tested in modules allowing the choice of an exposed or shielded area. During the test, the ticks were exposed to electro-magnetic radiation of 900MHz for 24h. The position of the individuals was recorded and we evaluated the obtained data statistically. We observed a significant preference to the exposed area in both sexes of I. ricinus. Males of D. reticulatus and D. marginatus also showed an affinity to radiation, but not females of both species, nor females and males of H. inermis. The results of the study support the assumption that ticks perceive the electromagnetic field and the observed differences in their response have the potential to help understand the mechanism of perception.

Discrete characteristics of instantaneous frequency of EMR induced by coal and rock fracture

To reveal the discrete characteristics of the instantaneous frequency of the electromagnetic radiation (EMR) waveform induced by coal and rock fracture, the uniaxial compression experiments for coal and rock samples were carried out, and the EMR signals with full waveform were acquainted and stored. The empirical wavelet transform is used to filter and de-noise the EMR waveform, and then the short-time Fourier transform is used to analyze the time-frequency characteristics of the waveform. The discrete characteristics of the instantaneous frequency with a larger amplitude and the relationship between the centroid frequencies and peak-to-peak values (Vpps) of the EMR waveforms are statistically analyzed. The results show that the centroid frequency of 0–100 kHz is negatively correlated with the Vpp, and the relationship between them shows a logarithm function relation. The instantaneous frequency of the EMR waveform of coal and rock fracture has significant discrete characteristics. In detail, for the rock sample, the instantaneous frequencies with relatively large amplitude are mainly 4.5 kHz, 19.5 kHz, 22.0 kHz, and 27.5 kHz; for the coal sample, the instantaneous frequencies are mainly 1.0 kHz, 4.5 kHz, 9.0 kHz, and 74.0 kHz. This discrete characteristic is determined by the natural properties and fracture characteristics of the sample. Compared with the homogeneous rock samples, the internal cracks of the coal samples are well developed and show strong anisotropy, resulting in the discrete characteristics of the instantaneous frequency being relatively weaker. The findings have certain guiding significance for optimizing the design of the EMR monitoring frequency band and improving the pertinence and accuracy of the monitoring and early warning for coal and rock dynamic disasters.

Extremely Low Frequency Electromagnetic Field Radiation (50 Hz, 200 µT & 300 µT) to Increase Edamame Productivity and Safety Risks to Health

More and more research results report that exposure to Extremely Low Frequency (ELF) magnetic fields at low intensity can increase cell proliferation. The ELF magnetic field interacts directly with cell membranes, capable of increasing intracellular calcium and under certain conditions affecting cell proliferation. This study wanted to prove the potential of ELF magnetic field radiation with intensity of 200 µT and 300 µT on edamame productivity. The research design used a completely randomized design. The research sample was 250 edamame soybeans of the Ryokkoh-75 variety which were divided into 5 groups. Samples were soaked in warm water for 3 hours and then cured for about 12 hours and exposed to ELF magnetic fields of intensity of 200 µT and 300 µT with variations in exposure time of 60 minutes and 120 minutes. Then it is seeded and planted in the field. Observation of the number of edamame pods and the number of pods was carried out when they were already fruiting, namely day 29 to day 43. The results of the study proved that the number of pods and the number of edamame pods in the group exposed to the 200 µT and 300 µT ELF magnetic fields was higher than the control. It appears that the highest number of fruits and pods occurred in the sample group exposed to the ELF magnetic field intensity of 300 µT. The conclusion is that exposure to the ELF magnetic field intensity of 300 µT has the potential to increase edamame productivity

In-situ gas content evaluation of producing coalbed methane reservoirs based on electromagnetic radiation anomalies

In-situ gas content evaluation in coalbed methane (CBM) reservoirs is one of the most critical issues in the fields of gas disaster forecasting, stable production and resource control. Traditionally, the methods for the CBM assessment of coal reservoirs have relied on laboratory evaluation, well testing, logging and seismics, which are very expensive and inefficient in terms of accurate regional investigation. This paper proposes a non-contact in-situ evaluation method based on electromagnetic radiation (EMR) anomaly. Electrical-mechanical coupling models have been theoretically investigated based on the Super-Low Frequency (SLF) electromagnetic radiation (EMR) generating process. Model simulation and field survey results have further demonstrated that in-situ EMR anomalies’ collection with a level of 0.01–1000 nT on the surface is feasible. Furthermore, using the BD-6 SLF detector with built-in processing algorithms such as background field removal and the ensemble empirical mode decomposition (EEMD), the natural-source EMR extraction method is proposed in support of statistical models established to estimate the in-situ reservoir pressure. Using nonlinear cubic polynomials, an ideal model of reservoir pressure and the normalized EMR signals is built, satisfying theoretical predictions (the maximum R-square value is above 0.83). The “Reservoir Pressure (RP)-EMR"-based approach for evaluating gas content in relation to producing reservoirs is then further suggested. In the study area, the proposed methodology is effectively used with a good ability for the local CBM resource monitoring of 6.4 × 1010m3. From August 2011 to October 2011, the estimated gas production is 6.3 million m3 (the actual gas production is 5.9 million m3). Therefore, this promising method is expected to be one of the most effective ways for industrials to realize the CBM evaluation in producing reservoirs.

Ternary mixture thermochromic microcapsules for visible light absorption and photothermal conversion energy storage

Thermochromic microcapsules with photothermal conversion properties were prepared by melamine urea formaldehyde resin (MUF) coated ternary mixtures (color coupler, color developer and solvent). The particle characteristics, chemical structure, heat storage properties, discoloration effect, thermal cycling durability and photothermal conversion properties of microcapsules were analyzed and characterized by ESEM and LPSA, FTIR and XRD, DSC and TGA, SC-10 and UV–Vis, thermal cycling test and solar simulator. The thermochromic and photothermal conversion mechanisms of microcapsules were analyzed. The results showed that the thermochromic microcapsules with a core material ratio of (1:3:60) have the best comprehensive performance. The chromatic aberration value of RTPCMs-C3 and RTPCMs-O3 is 23.35 and 12.14, respectively, which has a certain visible light absorption capacity and excellent thermal cycle durability. The photothermal conversion efficiencies of RTPCMs-C3 and RTPCMs-O3 under simulated solar irradiation were 55.4% and 72.9%, respectively. The conjugated organic polymer core material selectively absorbs the electromagnetic radiation frequency which are matching its internal electronic transition, causing the atomic lattice vibration to generate heat. The heat changes the state of the core material and dissociates the internal conjugated double bonds, eventually causing the microcapsules to change color.

Effect of extremely low-frequency electromagnetic radiation on pregnancy outcome: A meta-analysis.

Extremely low-frequency electromagnetic radiation (ELF-EMF) are generated by electrical devices and power systems (1 to 300 Hz). Although several studies have demonstrated that ELF-EMF may beassociated with an increased risk of adverse pregnancy outcomes, other studies have shown no evidence of associations. This meta-analysis was conducted to assess the effect of extremely low frequency electromagnetic radiation on pregnancy outcomes. The following electronic bibliographic databases were searched to identify relevant studies: PubMed, Web Of Science, Cochrane library, Embase, EBSCO. In addition, the manual retrieval of relevant references was conducted as a supplement. Select all eligible studies published from Database construction library to March 10, 2021. Search type for queue research on influence of electromagnetic field radiation on pregnancy results. Data were screened and extracted independently by two researchers. Review Manager 5.3 software was used for the meta-analysis. There was no significant increase in the risk of miscarriage, stillbirth, birth defects and preterm delivery in the pregnant women who lived near the electromagnetic fields compared with the control group. Conclusions: No correlation has been found between maternal ELF-EMF exposure and miscarriage, stillbirth, neonatal birth defects and preterm delivery, while the effects on small gestational age and low birth weight are still uncertain. Related research with high-quality large samples and different regions are still needed for further verification.

MARIE 2.0: A Perturbation Matrix Based Patient-Specific MRI Field Simulator.

High static field MR scanners can produce human tissue images of astounding clarity, but rely on high frequency electromagnetic radiation that generates complicated in-tissue field patterns that are patient-specific and potentially harmful. Many such scanners use parallel transmitters to better control field patterns, but then adjust the transmitters based on general guidelines rather than optimizing for the specific patient, mostly because computing patient-specific fields was presumed far too slow. It was recently demonstrated that the combination of fast low-resolution tissue mapping and fast voxel-based field simulation can be used to perform a patient-specific MR safety check in minutes. However, the field simulation required several of those minutes, making it too slow to perform the dozens of simulations that would be needed for patient-specific optimization. In this paper we describe a compressed-perturbation-matrix technique that nearly eliminates the computational cost of including complex coils (or coils and shields) in voxel-based field simulation of tissue, thereby reducing simulation time from minutes to seconds. The approach is demonstrated on a wide variety of head+coil and head+coil+shield configurations, using the implementation in MARIE 2.0, the latest version of the open-source MR field simulator MARIE.

Analysis of electromagnetic response of cells and lipid membranes using a model-free method

Electromagnetic radiation (EMR) is omnipresent on earth and may interact with the biological systems in diverse manners. But the scope and nature of such interactions remain poorly understood. In this study, we have measured the permittivity of cells and lipid membranes over the EMR frequency range of 20 Hz to 4.35 × 1010 Hz. To identify EMR frequencies that display physically intuitive permittivity features, we have developed a model-free method that relies on a potassium chloride reference solution of direct-current (DC) conductivity equal to that of the target sample. The dielectric constant, which reflects the capacity to store energy, displays a characteristic peak at 105–106 Hz. The dielectric loss factor, which represents EMR absorption, is markedly enhanced at 107–109 Hz. The fine characteristic features are influenced by the size and composition of these membraned structures. Mechanical disruption results in abrogation of these characteristic features. Enhanced energy storage at 105–106 Hz and energy absorption at 107–109 Hz may affect certain membrane activity relevant to cellular function.