Frozen Period Research Articles

Seasonal Variations of Ice-Covered Lake Ecosystems in the Context of Climate Warming: A Review

The period of freezing is an important phenological characteristic of lakes in the Northern Hemisphere, exhibiting higher sensitivity to regional climate changes and aiding in the detection of Earth’s response to climate change. This review systematically examines 1141 articles on seasonal frozen lakes from 1991 to 2021, aiming to understand the seasonal variations and control conditions of ice-covered lakes. For the former, we discussed the physical structure and growth characteristics of seasonal ice cover, changes in water environmental conditions and primary production, accumulation and transformation of CO2 beneath the ice, and the role of winter lakes as carbon sources or sinks. We also proposed a concept of structural stratification based on the differences in physical properties of ice and solute content. The latter provided an overview of the ice-covered period (−1.2 d decade−1), lake evaporation (+16% by the end of the 21st century), the response of planktonic organisms (earlier spring blooming: 2.17 d year−1) to global climate change, the impact of greenhouse gas emissions on ice-free events, and the influence of individual characteristics such as depth, latitude, and elevation on the seasonal frozen lakes. Finally, future research directions for seasonally ice-covered lakes are discussed. Considering the limited and less systematic research conducted so far, this study aims to use bibliometric methods to synthesize and describe the trends and main research points of seasonal ice-covered lakes so as to lay an important foundation for scholars in this field to better understand the existing research progress and explore future research directions.

Analysis of bacterial community structure, functional variation, and assembly mechanisms in multi-media habitats of lakes during the frozen period

Ice, water, and sediment represent three interconnected habitats in lake ecosystems, and bacteria are crucial for maintaining ecosystem equilibrium and elemental cycling across these habitats. However, the differential characteristics and driving mechanisms of bacterial community structures in the ice, water, and sediments of seasonally frozen lakes remain unclear. In this study, high-throughput sequencing technology was used to analyze and compare the structure, function, network characteristics, and assembly mechanisms of bacterial communities in the ice, water, and sediment of Wuliangsuhai, a typical cold region in Inner Mongolia. The results showed that the bacterial communities in the ice and water phases had similar diversity and composition, with Proteobacteria, Bacteroidota, Actinobacteria, Campilobacterota, and Cyanobacteria as dominant phyla. The bacterial communities in sediments displayed significant differences from ice and water, with Chloroflexi, Proteobacteria, Firmicutes, Desulfobacterota, and Acidobacteriota being the dominant phyla. Notably, the bacterial communities in water exhibited higher spatial variability in their distribution than those in ice and sediment. This study also revealed that during the frozen period, the bacterial community species in the ice, water, and sediment media were dominated by cooperative relationships. Community assembly was primarily influenced by stochastic processes, with dispersal limitation and drift identified as the two most significant factors within this process. However, heterogeneous selection also played a significant role in the community composition. Furthermore, functions related to nitrogen, phosphorus, sulfur, carbon, and hydrogen cycling vary among bacterial communities in ice, water, and sediment. These findings elucidate the intrinsic mechanisms driving variability in bacterial community structure and changes in water quality across different media phases (ice, water, and sediment) in cold-zone lakes during the freezing period, offering new insights for water environmental protection and ecological restoration efforts in such environments.

Vertical Distribution Characteristics and Ecological Risk Assessment of Mercury and Arsenic in Ice, Water, and Sediment at a Cold-Arid Lake.

Mercury and arsenic are two highly toxic pollutants, and many researchers have explored the effects of the two substances on the environment. However, the research content of toxic substances in frozen periods is relatively small. To explore the spatial and vertical distribution of mercury and arsenic in the ice, water, and sediments of Wuliangsuhai Lake under ice conditions, and to assess the harm degree of the two toxic substances to human beings. We collected the ice, water, and sediments of the lake in December 2020, and tested the contents of Hg and As. The single-factor pollution index method, the local cumulative index method, and the ecological risk coding method were used to assess the pollution status in these three environmental media, and the Monte Carlo simulation combined with the quantitative model recommended by USEPA was used to assess the population health risk. The results showed that (1) The average single-factor pollution values of Hg and As in water were 0.367 and 0.114, both pollutants were at clean levels during the frozen period. (2) The mean Igeo values of Hg and As were 0.657 and -0.948. The bioavailability of Hg in the sediments of Wuliangsuhai Lake during the frozen period was high, and its average value was 7.8%, which belonged to the low-risk grade. The bioavailability of As ranged from 0.2% to 3.7%, with an average value of 1.3%. (3) Monte Carlo simulation results indicate acceptable levels of health risks in both water and ice. This study preliminarily investigated the distribution characteristics of toxic substances and their potential effects on human health in lakes in cold and arid regions during the frozen period. It not only clarified the pollution characteristics of lakes in cold and arid regions during the frozen period, but also provided beneficial supplements for the ecological protection of lake basins. This study lays a foundation for further environmental science research in the region in the future.

Overwintering under ice: A novel observation for an Australian freshwater turtle.

Frozen water bodies provide a physiological challenge to fauna by physically limiting access to atmospheric oxygen. To tolerate low temperatures, reptiles use brumation as a physiological strategy in winter. Cryptodira vary in their tolerance to freezing conditions but the extent of tolerance in pleurodirans is largely unknown. Australia's freshwater turtles inhabit warmer regions with less severe winters and have well-developed mechanisms to cope with high temperatures and drying waterbodies, rather than extreme cold tolerance. Chelodina longicollis is a widespread Australian freshwater turtle species that tolerates high temperatures and desiccation during hot, dry periods while also undergoing brumation during winter months. Despite extensive research, limited observations exist on their behaviour during severe winter periods at the extremes of their range. In an 11-month tracking study, we monitored adult C. longicollis, noting their movements, locations, and temperature weekly. We observed an adult female C. longicollis which, during a seven-month period within a single creek pool, survived brumation in extreme cold water including a 15-day period of total freezing of the surface water. After the ice melted following a rain event, the turtle was recaptured alive. This marks the first observation of brumation for an Australian chelid species under ice.

Freeze-thaw characteristics and deformation monitoring of topsoil in highland pastoral areas under the influence of snow accumulation

Soil freeze–thaw processes and snowmelt infiltration have a significant impact on the hydrological cycle and ecosystem productivity in alpine pastoral areas. To investigate the driving mechanism of snow on soil freeze-thaw processes, a meteorological station was established in the southern part of Namatso Lake, Tibet to collect environmental data. The study included analyzing the relationship between soil temperature and humity, as well as assessing soil freeze-thaw characteristics and the evolution of soil frost heave over time. The freeze-thaw frequency was significantly 43% lower at the surface soil, and the freeze-thaw intensity decreased when the ground was snow-covered. During the initial freezing period, precipitation and soil humidity remain relatively low, and soil moisture decreases linearly with decreasing soil temperature, which occurs in the phenomenon of soil freeze-shrink. During the initial thawing period, snowmelt infiltration impacts soil humidity, and soil frost heave increases logarithmically, reaching a maximum of 5.2 mm, driven by freeze-thaw. Soil moisture decreases under topsoil evaporation in the later stage and again follows a linear trend with the soil temperature. This study not only reveals the correlation between soil temperature and humidity during different freeze-thaw periods but also enhances the understanding of soil deformation patterns under the influence of snow accumulation.

Cryoprotective role of vacuum infused inulin on the quality of artichoke: Interactive effects of freezing, thawing and storage period

Freezing of artichoke is a promising alternative to storing it in brine and canning. The perishable vegetable was vacuum infused with inulin to improve freezing tolerance. Artichokes with and without inulin were frozen by static, air blast and individual quick freezing (IQF) methods and thawed by microwave, 25 °C and 4 °C temperature levels at each month of 6-months storage. Process conditions were evaluated by multivariate analysis of variance (MANOVA) and were found significant on the quality parameters. Inulin infusion better conserved the aw, color, texture, ascorbic acid and overall integrity of artichokes during frozen storage. Inulin incorporation and IQF showed mutual positive effect on drip loss. Polyphenol oxidase (PPO) activity values fitted to 2nd order kinetic and the highest residuals were determined in static freezing. PPO showed alleviating effect on total phenolic content. Vacuum impregnation caused a color difference prior to freezing, but was found effective for maintaining color during storage. As a result, the use of quick freezing techniques together with the addition of cryoprotectant was effective in the preservation of artichoke quality attributes during frozen storage.

Seasonal freeze‒thaw processes impact microbial communities of soil aggregates associated with soil pores on the Qinghai–Tibet Plateau

BackgroundSeasonal freeze‒thaw (FT) processes alter soil formation and cause changes in soil microbial communities, which regulate the decomposition of organic matter in alpine ecosystems. Soil aggregates are basic structural units and play a critical role in microbial habitation. However, the impact of seasonal FT processes on the distribution of microbial communities associated with soil pores in different aggregate fractions under climate change has been overlooked. In this study, we sampled soil aggregates from two typical alpine ecosystems (alpine meadow and alpine shrubland) during the seasonal FT processes (UFP: unstable freezing period, SFP: stable frozen period, UTP: unstable thawing period and STP: stable thawed period). The phospholipid fatty acid (PLFA) method was used to determine the biomass of living microbes in different aggregate fractions.ResultsThe microbial biomass of 0.25–2 mm and 0.053–0.25 mm aggregates did not change significantly during the seasonal FT process while the microbial biomass of > 2 mm aggregates presented a significant difference between the STP and UTP. Bacterial communities dominated the microbes in aggregates, accounting for over 80% of the total PLFAs. The microbial communities of soil aggregates in the surface layer were more sensitive to the seasonal FT process than those in other soil layers. In the thawing period, Gram positive bacteria (GP) was more dominant. In the freezing period, the ratio of Gram-positive to Gram-negative bacterial PLFAs (GP/GN) was low because the enrichment of plant litter facilitated the formation of organic matter. In the freezing process, pores of 30–80 μm (mesopores) favored the habitation of fungal and actinobacterial communities while total PLFAs and bacterial PLFAs were negatively correlated with mesopores in the thawing process.ConclusionsThe freezing process caused a greater variability in microbial biomass of different aggregate fractions. The thawing process increased the differences in microbial biomass among soil horizons. Mesopores of aggregates supported the habitation of actinobacterial and fungal communities while they were not conducive to bacterial growth. These findings provide a further comprehension of biodiversity and accurate estimation of global carbon cycle.

Effects of the freeze–thaw process on sources and pathways of subsurface flow in an alpine hillslope on the northeastern Qinghai-Tibet Plateau

The impact of the freeze–thaw process on the active layer is reflected in the changed subsurface flow (SSF) process in cold alpine regions. Identifying sources and pathways of SSF in the freeze–thaw process is critical but difficult, and the related dominant factors and mechanisms are still unknown. In this paper, the effective identification and analysis of SSF are promoted based on field sampling data from the thawing (June) to freezing (September) period of 2022 in the Qinghai Lake basin on the northeastern Qinghai-Tibetan Plateau. By the proposed method with a high sampling frequency and refined sampling spatial scale, the sources and pathways of SSF are clearly identified. The results are as follows: (1) The soil temperature is considered the most fundamental factor affecting the SSF pathways, it influences water infiltration to the deep layer and the effect is extended to the saprolite and weathered bedrock layers. (2) Thawing promotes water to infiltrating into deep layer. 30 cm soil water contributes the most to SSF (2 %–86 %) in the thawing period, while the contribution difference of the water from the 30 cm, 60 cm, and 90 cm layers is small (ranging from 32 %–33 %, 24 %–26 %, and 32 %–35 %, respectively) in the thawed period. (3) Meanwhile, the soil water from different slope positions contribute differently to SSF, and the SSF from deep soil layer is transit in prolong paths and depths. It is caused by the out-of-sync water transit process in the hillslope. With continuing climate warming, we propose that the differences in the water sources of SSF across soil layers may decrease, while the differences in the transit processes of SSF across soil layers may increase.

Cryoprotective effect of epigallocatechin gallate replacing sucrose on Hypophythalmichthys molitrix surimi during frozen storage.

The present study aimed to investigate the cryoprotective effect of epigallocatechin gallate (EGCG) replacing sucrose on surimi during frozen storage. Substitution or partial substitution of 0.1% EGCG for sucrose (1.5%) was added to surimi, and the surimi samples without and with commercial cryoprotectants (4% sucrose and 4% sorbitol) were used as the control group. The results obtained suggest that, with the increase in frozen storage time, the structural performance of surimi protein gradually weakened (e.g. the decrease in the surface hydrophobicity, the increase in the total sulfhydryl and solubility, and the protein myosin heavy chain bands became shallow) and surimi gel quality gradually deteriorated (e.g. the decrease in water-holding capacity, gel strength and all texture profile attributes). However, compared with the other three group surimi samples during the frozen period, the surimi proteins with partial replacement of sucrose by EGCG had a higher total sulfhydryl group content and solubility of proteins, as well as lower surface hydrophobicity of protein, suggesting that the addition of EGCG as a partial substitute for sucrose can enhance the antifreeze ability of surimi. Meanwhile, the surimi gel with the partial replacement of sucrose by EGCG had a higher water retention capacity, gel strength and texture attributes (e.g. hardness, springiness, cohesiveness, chewiness, and resilience), indicating that the addition of EGCG as a partial substitute for sucrose can inhibit the deterioration of surimi gel quality. Overall, EGCG partially replacing sucrose can play an alternative cryoprotectant with a lower sweetness to prevent the quality of surimi from deteriorating. © 2024 Society of Chemical Industry.

Contribution of standardized indexes to understand groundwater level fluctuations in response to meteorological conditions in cold and humid climates

Understanding the fluctuations in groundwater levels in response to meteorological conditions is challenging, especially given the slow transit time associated with groundwater reservoirs and the short duration of time series for groundwater levels. Nevertheless, this knowledge is crucial for water resource management, especially given that global warming will drastically impact the hydrological dynamics in cold and humid climates. The objective of this work was to quantify how standardized indexes contribute to understanding groundwater level fluctuations in response to meteorological conditions in cold and humid climates and with short time series (10 to 23 years). The relationships between the standardized precipitation index (SPI), standardized temperature index (STI), global climate indexes, and standardized groundwater index (SGI) were analyzed. The reactivity of groundwater levels was examined between 2000 and 2022 using groundwater level measurements from 152 wells located between 46 °N and 52 °N in the province of Quebec (Canada). The results showed that the available time series were sufficient to provide new insights into the role of precipitation and temperature on groundwater fluctuations, demonstrating the usefulness of the indexes. One of the main contributions of this study was that hydrogeological systems in cold and humid climates go through an annual reset due to the prolonged freezing period. This annual reset was one of the drivers isolating year-to-year hydrogeological conditions, contributing to short-duration droughts.

Insights into the thermal insulation capability of a new polyurethane polymer subgrade material: An in-situ field test on the Qinghai–Tibet highway

The frost heave and thaw settlement of subgrades in permafrost regions severely threaten highway safety during repeated freeze–thaw cycles. However, these freeze–thaw cycles are difficult to overcome because of the lack of thermal insulation materials (or structures). To fill this gap, we developed a polyurethane (PU) polymer material with low thermal conductivity and designed a double–layer PU–grouted structure. The water–vapor–thermal coupling governing equations of the grouted subgrade were established. A numerical simulation was conducted using the finite element software COMSOL Multiphysics. Ten-month in-situ tests were conducted on the Qinghai–Tibet Highway from November 2021 to August 2022 to investigate the thermal insulation properties of the grouted subgrade. The results indicated that the PU exhibited excellent thermal insulation performance and considerably reduced the disturbance depth of the air temperature. Freeze and thaw depths were reduced by 0.65 and 1.65 m, respectively. During the freeze period, the surface and deep layers of the subgrade showed a temperature difference of 6 °C due to the upper PU layer, preventing 32.4 % of the liquid water from being frozen. The upper PU layer caused a temperature difference of up to 7 °C between the surface and the deep layers of the subgrade during the thawing period. Water evaporation was prevented by the upper PU layer, and water gathered between the grouted layers, which was defined as the “pot-cover effect.” The pot cover effect significantly reduced the soil temperature and maintained a stable temperature in the deeper subgrade during thawing.

Seasonal Freezing Drives Spatiotemporal Dynamics of Dissolved Organic Matter (DOM) and Microbial Communities in Reclaimed Water-Recharged River

Although reclaimed water (RW) has become a promising alternative source for alleviating water shortage in arid and semiarid regions, the ecological risks it poses to the receiving water bodies remain largely unknown. Dissolved organic matter (DOM) is crucial for affecting the quality of RW and strongly influences bacterial communities (BCs) in aquatic ecosystems. In this study, we aimed to unravel the role of DOM signatures on the spatiotemporal composition of microbial communities (MCs) in a seasonally ice-sealed urban river that had been chronically replenished by RW. We found that discharging RW resulted in elevated DOM levels in the receiving river. Notably, an increase of 10% in protein-like substances was revealed. The differences between compositional characteristics of DOM and the abundance of riverine BCs between freezing and non-freezing periods were revealed. In the freezing season, humic-like components, aromaticity, and hydrophobicity of DOM were more significant, and bacterial taxa such as Bacteriodetes and Flavobacterium were increased, while Proteobacteria was decreased. Similarly, co-occurrence network analysis revealed an enhanced interplay between DOM and BCs at the same time. However, Klebsiella pneumoniae markedly decreased during the ice-sealed period. These results suggest that variations in DOM characteristics have remarkable impacts on the dynamics of aquatic BCs, which points to the need for a DOM−oriented RW quality monitoring strategy.

Permafrost Carbon: Progress on Understanding Stocks and Fluxes Across Northern Terrestrial Ecosystems

AbstractSignificant progress in permafrost carbon science made over the past decades include the identification of vast permafrost carbon stocks, the development of new pan‐Arctic permafrost maps, an increase in terrestrial measurement sites for CO2 and methane fluxes, and important factors affecting carbon cycling, including vegetation changes, periods of soil freezing and thawing, wildfire, and other disturbance events. Process‐based modeling studies now include key elements of permafrost carbon cycling and advances in statistical modeling and inverse modeling enhance understanding of permafrost region C budgets. By combining existing data syntheses and model outputs, the permafrost region is likely a wetland methane source and small terrestrial ecosystem CO2 sink with lower net CO2 uptake toward higher latitudes, excluding wildfire emissions. For 2002–2014, the strongest CO2 sink was located in western Canada (median: −52 g C m−2 y−1) and smallest sinks in Alaska, Canadian tundra, and Siberian tundra (medians: −5 to −9 g C m−2 y−1). Eurasian regions had the largest median wetland methane fluxes (16–18 g CH4 m−2 y−1). Quantifying the regional scale carbon balance remains challenging because of high spatial and temporal variability and relatively low density of observations. More accurate permafrost region carbon fluxes require: (a) the development of better maps characterizing wetlands and dynamics of vegetation and disturbances, including abrupt permafrost thaw; (b) the establishment of new year‐round CO2 and methane flux sites in underrepresented areas; and (c) improved models that better represent important permafrost carbon cycle dynamics, including non‐growing season emissions and disturbance effects.

Characteristics of Soil Moisture and Heat Change during Freeze–Thaw Process in the Alpine Grassland of Duogerong Basin in the Source of the Yellow River

To deeply understand the characteristics of soil freeze–thaw water–heat change in the alpine grassland in the Duogerong Basin of the Yellow River source, the soil water–heat profile change monitoring was carried out based on the field monitoring station in the Duogerong Basin of the Yellow River source. By analyzing the comprehensive monitoring data from September 2022 to September 2023, the characteristics of the soil temperature and water content changes in the freeze–thaw cycle of the alpine grassland in the Duogerong Basin at the source of the Yellow River were explored. The results showed that the temperature and water content of each layer of the soil profile changed periodically, and the range of change was negatively correlated with the depth. The annual freeze–thaw process at the observation site is divided into five stages: 31 October to 3 November is the short initial freezing period, 4 November to 18 April is the stable freezing period, 19 April to 26 April is the early ablation period, 27 April to 30 April is the late ablation period, and 1 May to 30 October is the complete ablation period. The maximum soil freezing depth during the observation period was about 250 cm. Soil temperature and moisture content change affect each other; soil water is essential in heat transfer, and the two correlate well. The research results provide theoretical support for further understanding the characteristics of soil hydrothermal changes during the freeze–thaw process in the alpine grassland permafrost area at the source of the Yellow River.

Effects of Various Non-genetic Factors on Fertility Traits in Murrah Bulls in an Organized Herd

Background: In a herd going through improvement by selective breeding, bulls contribute more than half of the genetic gain due to higher selection intensity and wider coverage through AI. On the other side, meritorious sires might have a lower fertility due to various reasons which would render them less fit in their contribution to genetic improvement. Thus dairy buffalo breeders must find a delicate balance between production and reproduction superiority for selection of sires, for which an accurate assessment of genetic and non-genetic component of conception is essential. Methods: Present study was conducted on thirty five years data comprising of 5349 AI records on 1302 Murrah buffaloes using 86 bulls maintained and semen analysis of cryopreserved semen samples of 130 Murrah maintained at ICAR-National Dairy Research Institute Karnal were analyzed to study bull conception rate and sperm quality traits. Data were classified into four periods; four seasons of AI viz., Summer (April-June), rainy (July-September), autumn (October-November), winter (December-March); five parity (first, second, third, fourth, fifth and above) and five female AI number (1,2,3,4 and ³ 5 AI) to study the conception rate. Age at freezing (Years), period of freezing and season of freezing were analysed to observe their effects on semen quality traits. Mixed model analysis (SAS 9.3) and the analysis of variance were used to see the effects of various factors on conception rate as well as on semen quality parameters. Result: The analysis of variance revealed that period of AI and season of AI had significant (P less than 0.05) effect on conception rate while parity and female AI number were not found to affect the conception rate of bulls. For seminal parameters, acrosome integrity and HOST were significantly affected by age at freezing. Season affected all the semen quality traits while the effect of period of freezing was found to be non-significant for all the traits. Over all autumn season was found to be most suitable with respect to better seminal parameters. Considering season of AI, rainy season (July-September) had the lowest conception rate. The overall conception rate (%) in the study was 38.43±0.99; where Sires showed a wide variation for conception rate ranging from 20.00% to 60.46%. Concluding with the fact that there exists variation among bull fertility that can be utilized for not only herd conception rates but also be used as a criteria for bull selection and evaluation.

A Review on the Driving Mechanism of the Spring Algal Bloom in Lakes Using Freezing and Thawing Processes

Spring algal blooms in mid–high-latitude lakes are facing serious challenges such as earlier outbreaks, longer duration, and increasing frequency under the dual pressure of climate warming and human activities, which threaten the health of freshwater ecosystems and water security. At present, the freeze-thaw processes is the key to distinguishing spring algal blooms in mid- to high-latitude lakes from low-latitude lakes. Based on the visualization and an analysis of the literature in the WOS database during 2007–2023, we clarified the driving mechanism of the freeze-thaw process (freeze-thaw, freeze-up, and thawing) on spring algal bloom in lakes by describing the evolution of the freeze-thaw processes on the nutrient migration and transformation, water temperature, lake transparency and dissolved oxygen, and physiological characteristics of algae between shallow lakes and deep lakes. We found that the complex phosphorus transformation process during the frozen period can better explain the spring-algal-bloom phenomenon compared to nitrogen. The dominant species of lake algae also undergo transformation during the freeze-thaw process. On this basis, the response mechanism of spring algal blooms in lakes to future climate change has been sorted out. The general framework of “principles analysis, model construction, simulation and prediction, assessment and management” and the prevention strategy for dealing with spring algal bloom in lakes have been proposed, for which we would like to provide scientific support and reference for the comprehensive prevention and control of spring algal bloom in lakes under the freezing and thawing processes.

Co-occurrence network analysis and community composition of bacteria and cyanobacteria in various types of lakes during frozen period

Co-occurrence network analysis and community composition of bacteria and cyanobacteria in various types of lakes during frozen period

Monitoring of Lake Ice Phenology Changes in Bosten Lake Based on Bayesian Change Detection Algorithm and Passive Microwave Remote Sensing (PMRS) Data.

Lake ice phenology (LIP), hiding information about lake energy and material exchange, serves as an important indicator of climate change. Utilizing an efficient technique to swiftly extract lake ice information is crucial in the field of lake ice research. The Bayesian ensemble change detection (BECD) algorithm stands out as a powerful tool, requiring no threshold compared to other algorithms and, instead, utilizing the probability of abrupt changes to detect positions. This method is predominantly employed by automatically extracting change points from time series data, showcasing its efficiency and accuracy, especially in revealing phenological and seasonal characteristics. This paper focuses on Bosten Lake (BL) and employs PMRS data in conjunction with the Bayesian change detection algorithm. It introduces an automated method for extracting LIP information based on the Bayesian change detection algorithm. In this study, the BECD algorithm was employed to extract lake ice phenology information from passive microwave remote sensing data on Bosten Lake. The reliability of the passive microwave remote sensing data was further investigated through cross-validation with MOD10A1 data. Additionally, the Mann-Kendall non-parametric test was applied to analyze the trends in lake ice phenology changes in Bosten Lake. Spatial variations were examined using MOD09GQ data. The results indicate: (1) The Bayesian change detection algorithm (BCDA), in conjunction with PMRS data, offers a high level of accuracy and reliability in extracting the lake ice freezing and thawing processes. It accurately captures the phenological parameters of BL's ice. (2) The average start date of lake ice freezing is in mid-December, lasting for about three months, and the start date of ice thawing is usually in mid-March. The freezing duration (FD) of lake ice is relatively short, shortening each year, while the thawing speed is faster. The stability of the lake ice complete ice cover duration is poor, averaging 84 days. (3) The dynamic evolution of BL ice is rapid and regionally distinct, with the lake center, southwest, and southeast regions being the earliest areas for ice formation and thawing, while the northwest coastal and Huang Shui Gou areas experience later ice formation. (4) Since 1978, BL's ice has exhibited noticeable trends: the onset of freezing, the commencement of thawing, complete thawing, and full freezing have progressively advanced in regard to dates. The periods of full ice coverage, ice presence, thawing, and freezing have all shown a tendency toward shorter durations. This study introduces an innovative method for LIP extraction, opening up new prospects for the study of lake ecosystem and strategy formulation, which is worthy of further exploration and application in other lakes and regions.

Root water uptake and water transport to above-ground organs compensate for winter water losses and prevent shoot dehydration in apple trees

Plants need to maintain a minimum moisture content in their tissues to preserve their living functions not only during the growing season but also during periods of dormancy. Whereas water uptake of trees during the growing season has been widely investigated, very little attention has been paid to tree water uptake and losses during winter dormancy. A study was therefore conducted aiming at quantifying root water uptake of apple trees in winter, comparing the water content of two groups of field-grown apple trees for two winter seasons. One group of trees was isolated from its roots by a transversal cut at the base of the trunk and the other was left intact. In an additional experiment, deuterium-enriched water was added to the soil of potted apple trees during winter dormancy, to monitor water uptake and translocation within the plant. The field experiment revealed incrementing moisture losses of annual shoots in late winter, driven by the rising evaporative demand of the atmosphere. Trees with unimpaired water transport systems compensated shoot water losses by root water uptake and internal translocation, whereas trees with severed trunks only partially mitigated losses by internal translocation from trunk and larger branches. Root water uptake during winter dormancy was also confirmed in the potted tree experiment with deuterium-enriched water. This work provides evidence that water flow along the soil-plant-atmosphere continuum in apple trees takes place also in winter, with possible impacts on tree survival during prolonged periods of soil or trunk freezing.

STUDY OF STRESS RESISTANCE OF OILSEED RADISH VARIETIES TO SUBZERO TEMPERATURES BY CHLOROPHYLL PHEOPHYTINIZATION

The article highlights the results of studying the peculiarities of the formation of stress resistance of 14 oilseed radish varieties of different ecological and geographical origin to subzero temperatures in the context of 5 basic phenological stages (cotyledon phase (BBCH 10), rosette phase (BBCH 14–16), stemming phase (BBCH 34–36), budding phase (BBCH 50–52) and flowering phase (BBCH 65–67)) with a temperature range of -1.0... -12.0 °C with an experimental step of 1.0 °C decrease based on the tested method of chlorophyll phytophytinization after the physiologically appropriate period of freezing of the calves. The peculiarities of stress reactions in terms of the proportion of phytophytinized leaf surface were investigated based on the processing of scanned images of leaf blades taken from living plants at the appropriate stages of their growth and development. The physiological aspects and literature sources on the assessment of temperature tolerance of cruciferous plant species, in particular radish subspecies, were analyzed and generalizations were made about the adaptive potential of oilseed radish as a species for the conditions of the Right-Bank Forest-Steppe of Ukraine. On the basis of a long-term period of research (2015–2022), the optimal physiological interval of the reaction of different genotypes of oil radish to subzero temperatures was estimated and the most stable phenological phases in relation to the frost resistance of plants were determined by the indicator short-term express reaction of leaf tissues based on the results of their freezing under controlled conditions. A dynamic series of oilseed radish varieties was determined in the direction of increasing the genotype stress resistance to subzero temperatures for the averaged values in the interval from the cotyledon phase to the flowering phase: Lybid, Zhuravka, Ramonta, Ivea, Nika, Prygazhunya, Sabina, Fakel, Rainbow, Alpha, Snizhana, Tambovchanka, IRGSGI Line, Olga. The influence of the total stress of oilseed radish plants based on the value of the hydrothermal coefficient (HTC) during the growing season on the determination of stress resistance to low temperatures was assessed and it was concluded that the combinatorial nature of the formation of pre-adaptive reactions of oilseed radish plants to low temperatures. At the same time, the nature of dynamic changes in the curve of phytophytinization of leaf tissue chlorophyll was determined, which can be used to indirectly analyze deviations in resistance to subzero temperatures during critical periods of growth and development of oilseed radish plants. Additionally, the dynamics of the formation of the leaf chlorophyll phytophytinization index in 14 oilseed radish varieties was evaluated with the detailing of this process at different phenostages of growth and development, and the level of lethality of this process at the corresponding value of the applied freezing temperatures was noted. The article formulates the prospects for further research in the field of optimization of diagnostics of oilseed radish plants stress resistance to subzero temperatures based on the new proline biochemical method of identification at the tissue level.