Freeze-thaw Effect Research Articles

Prediction of permanent deformation of subgrade soils under F-T cycles using SABO-optimized CNN-BiLSTM network

Accurately predicting the permanent deformation of subgrade soils under combined loading and seasonal freeze-thaw (F-T) effects is crucial for optimizing pavement design and maintenance planning in cold regions. However, existing empirical models have limitations in capturing the non-linear deformation behavior influenced by interacting factors. This study developed a deep learning framework, specifically a convolutional neural network-bidirectional long short-term memory (CNN-BiLSTM), for time-series prediction of subgrade strains. Laboratory tests established a comprehensive database to examine soil response across various conditions. Regression analysis revealed the constraints of traditional models. The optimized hybrid architecture directly learned patterns from experimental data, outperforming regression by 29−71 % based on error metrics. Sensitivity analysis confirmed that the model identified primary governing inputs consistent with theory. Hyperparameter tuning with the Subtraction-Average-Based Optimizer further enhanced performance. This data-driven methodology demonstrated the potential of advanced machine learning in simulating complex subsurface deformation behavior under compounding factors in seasonal frozen environments.

Experimental investigation of freeze–thaw effects on the micropore properties of expansive soil using NMR–SEM techniques

Experimental investigation of freeze–thaw effects on the micropore properties of expansive soil using NMR–SEM techniques

Comparative study of statistical computational approaches to investigate the degraded compressive strength of concrete under the freeze-thaw effect

The primary cause of frost damage and early failure in concrete structures is the repeated freezing and thaw cycles (FTC). The evolution of internal fractures and scaling at the surface of concrete can be used to assess the impact of frost damage. Surface scaling mechanisms and interior frost damage are contingent upon numerous environmental factors, including the rate of freezing, low temperature, and duration of the freezing point. However, evaluating the amount of strength loss of concrete material is a challenging factor to consider. This work explores the application of predictive modelling tools, including random forest (RF), multilayer perceptron (MLP), decision tree (DT), and bagging, to evaluate the degraded compressive strength (D-CS) of concrete. The models use five input variables: initial compressive strength (I-CS), water-to-cement ratio (W/C), FTC, minimum temperature (Tmin), and maximum temperature (Tmax), with D-CS as the output. Model performance was compared using mean absolute error (MAE), root mean square error (RMSE), and coefficient of determination (R²) metrics, with the bagging model achieving the highest predictive performance at 92 %. A 10-fold cross-validation approach validated the model's accuracy. The influence of each variable was assessed using SHapley Additive exPlanations (SHAP) analysis. Importantly, a user-friendly Graphical User Interface (GUI) was developed based on the models, making it easy for researchers and professionals to make predictions and thereby increasing the practicality and accessibility of this research. This study aids the research community in selecting appropriate models to forecast the strength of various concrete types, thereby enhancing the practical application of this research.

Study on the Dispersion Characteristics of Coal Complex Resistivity under the Effect of Liquid Nitrogen Cyclic Freeze-Thaw and Evaluation of Permeability Enhancement.

The effect of liquid nitrogen freeze-thaw fracturing on coal seams can be potentially evaluated by the complex resistivity method. The real part (Reρ) and the imaginary part (Imρ) of the complex resistivity and permeability of coal were determined under different cycle times and in different bedding directions. The reason for permeability enhancement was discussed, and the dispersion mechanism of complex resistivity during cyclic freeze-thaw fracturing was analyzed. The results indicated that (1) the complex resistivity parameters have a good response to the cycle times; Reρ, |Imρ|, and the dispersion degree (α) are positively correlated with cycle time; the fully polarized frequency (f p) of Reρ, the characteristic frequency (f c) of Imρ, and variation are negatively correlated with cycle time. (2) The difference in complex resistivity parameters between the vertical bedding direction and the parallel bedding direction is significant, and the difference in electrical properties of the bedding structure continuously decreases with the increase in cycle time. (3) Under the effect of liquid nitrogen cyclic freeze-thaw, a complex network of fractures in coal is formed, the anisotropic characteristics of coal are weakened, and effective conductive channels are damaged. The peak frost heave force decreases exponentially with the increase in cycle time, and the difference in bedding electrical properties gradually disappears. (4) Comparing the inversion degree of measured data with three conductive models, ρ0 and τ are selected as the optimum parameters for evaluating the effect of liquid nitrogen cyclic freeze-thaw. A logarithmic permeability evaluation model is constructed based on ρ0 and τ. This work provides a new perspective based on electrical detection for evaluating the permeability enhancement of coal during liquid nitrogen cyclic freeze-thaw.

Study on the damage mechanism of hydraulic concrete under the alternating action of freeze-thaw and abrasion

This study investigates the damage mechanism of hydraulic concrete under the alternating action of freeze–thaw (F) and abrasion (W) in cold regions. To this end, three groups of working conditions were established with a constant water-binder ratio. The mass loss and relative dynamic elastic modulus (RDEM) of each cycle were evaluated using the fast-freezing method and underwater method. The phase composition and relative content change of hydration products, pore characteristic parameters and pore size distribution, morphology and microstructure of interfacial transition zone (ITZ) were analyzed by X-ray diffraction (XRD), mercury intrusion porosimeter(MIP), scanning electron microscopyn (SEM) and image recognition technology, respectively. The results indicate that the alternating effect will accelerate the mass loss of concrete and the reduction of the RDEM compared with the single factor. Following four consecutive alternating cycles, the mass loss rates of working conditions W and F group were found to be 4.61 % and 97.66 % lower, respectively, than those of working condition F-W group. The RDEM damage coefficient (γ < 1) was also observed. At the micro level, the freeze-thaw effect will result in the formation of a considerable number of micropores and the expansion of microcracks, with the abrasion effect continuing to promote this process. In the process of alternating action, the dissolution of Ca(OH)2 by external water through microcracks and interconnected pores is accelerated. Concurrently, the pores and microcracks in the ITZ expand, the morphology is roughened, and the microstructure is complicated. The interfacial bonding ability and compactness of different material components decrease. Under the action of external disturbance, cement paste and aggregate fall off, resulting in mass loss and a decreased RDEM.

Analysis of Freeze-Thaw Damage of Cement Mortars Doped with Polyethylene Glycol-Based Form Stable Phase Change Materials.

The development of construction materials with the integration of phase change materials (PCMs) has been a topic of wide interest in the scientific community, especially in recent years, due to its positive impact on temperature regulation inside buildings. However, little is known about the behavior of materials doped with PCMs when exposed to accidental or severe environments. Currently, a large area of the planet experiences seasonal freeze-thaw effects, which impact the durability and performance of construction materials. Accordingly, the main objective of this study was to evaluate the damage caused by cyclic freeze-thaw actions on the behavior of a cement mortar, including a PEG-based form-stable PCM. An experimental methodology was developed based on the physical and mechanical characterization of mortars under normal operating conditions and after being subjected to freeze-thaw cycles. The results indicated that, under normal exposure conditions, the incorporation of aggregate functionalized with PCM led to a decrease in the mortar's water absorption capacity, compressive strength, and adhesion. However, its applicability has not been compromised. Exposure to freeze-thaw cycles caused a loss of mass in the specimens and a decrease in the compressive strength and adhesion capability of the mortar.

Evaluation and Limitations of the Novel Chemiluminescent Enzyme Immunoassay Technique for Measuring Total Tau Protein in the Cerebrospinal Fluid of Patients with Human Prion Disease: A 10-Year Prospective Study (2011-2020).

Recently, the investigation of cerebrospinal fluid (CSF) biomarkers for diagnosing human prion diseases (HPD) has garnered significant attention. Reproducibility and accuracy are paramount in biomarker research, particularly in the measurement of total tau (T-tau) protein, which is a crucial diagnostic marker. Given the global impact of the coronavirus disease pandemic, the frequency of measuring this protein using one of the world's fully automated assays, chemiluminescent enzyme immunoassay (CLEA), has increased. At present, the diagnosis and monitoring of neurological diseases mainly rely on traditional methods, but their accuracy and responsiveness are limited. There is limited knowledge of the accuracy of CLEA in tau measurements. We aimed to measure T-tau protein using CLEA and to elucidate its merits and limitations. We randomly selected 60 patients with rapidly progressive dementia, using ELISA and CLEA analysis of cerebrospinal fluid specimens. Additionally, we used Western blotting to detect the presence of 14-3-3 protein and employed real-time quaking-induced conversion (RT-QuIC) assays to analyze the same set of samples. Furthermore, we examined the correlation coefficient between ELISA and CLEA results in a subset of 60 samples. Moreover, using CLEA, we evaluated the diurnal reproducibility, storage stability, dilutability, and freeze-thaw effects in three selected samples. In 172 patients, 172 samples were extracted, with each patient providing only one sample, and a total of 88 (35 men and 53 women) tested positive for HPD in the RT-QuIC assay. In contrast, all CSF samples from the remaining 84 patients without HPD (50 men and 34 women) tested negative in the RT-QuIC assay. Both ELISA and CLEA showed perfect sensitivity and specificity (100%) in measuring T-tau protein levels. In addition, ELISA and CLEA are similar in terms of measurement sensitivity and marginal effect of detection extrema. CLEA analysis exhibited instability for certain samples with T-tau protein levels exceeding 2000 pg/mL, leading to low reproducibility during dilution analysis. Our findings indicate that CLEA outperforms ELISA in terms of diurnal reproducibility, storage stability, and freeze-thaw effects. However, ELISA demonstrated superior performance in the dilution assay. Therefore, it is imperative to develop innovative approaches for the dilution of biomarker samples for CLEA measurements during clinical trials.

Mechanical property and frost resistance of phase–change/NanoSiO2 concrete in a low–temperature environment

To study the effect of phase–change materials and nanomaterials as new composite materials on the mechanical properties and frost resistance of concrete, it is beneficial for low–carbon and environmentally friendly buildings to be achieved. The effects of freeze–thaw cycles and different preloading strains were considered. ICT scanning was performed, and the compression performance of composite–modified concrete (CMC) were tested. The internal pore structure and strength indicators of the CMC were analyzed during uniaxial compression. Meanwhile, by conducting uniaxial compression tests on CMC at different temperatures, changes in the mechanical properties during real–time freezing and thawing processes were obtained. The results demonstrated that the fractal dimension grows and the compressive performance of the CMC declines as the number of freeze–thaw cycles and preloading strain increases. The proportion of spheroids in the internal pore shape gradually decreases, whereas the proportions of rods, discs, and blades increases. CMC effectively delays the rise or fall of internal temperature during the freeze–thaw process and reduces its internal structural deterioration. Compared with the freeze–thaw effect, an increase in the preloading strain has a more notable impact on the compressive performance of CMC. A stress–strain constitutive model is proposed for a 10 % microencapsulated phase–change materials and a 1.5 % nanoSiO2 composite–modified concrete (10 mPCMs/1.5 NS CMC), influenced by temperature, through regression analysis. Meanwhile, under low temperatures, the degradation of mechanical properties of 10 mPCMs/1.5 NS CMC and the influence of different preloading strains are considered, leading to the development of stress–strain constitutive models for freeze–thaw cycles and different preloading conditions.

Effect of Freeze-Thaw on CBR in Soils with Different Gradation and Mineralogy

Freeze-thaw cycles are prevalent climatic phenomena with substantial effects on soils, leading to alterations in soil strength, stiffness, and hydraulic properties due to disruptions in the soil structure. With the ongoing climate change, weather patterns have grown progressively erratic, resulting in more frequent occurrences of extreme weather events, including heavy snowfall, intense rainfall, and windstorms, even in regions characterized typically with mild climates across the globe. The climate change can potentially threat man-made infrastructure constructed within or upon local soils, regardless of their susceptibility to freezing in temperate climates. The principal objective of this study is to assess the influence of freeze-thaw cycles on the California Bearing Ratio (CBR %) across 12 distinct soils with variations in granulometry and mineralogy. The freeze-thaw cycles resulted in a notable decrease in CBR (%) within the range of 40% to 70%. A strong inverse correlation with D50 was observed regarding the decrease in CBR (%). Nevertheless, it was discerned that the decrease in CBR (%) subsequent to freeze-thaw cycles varied among soil samples sharing identical D50 and liquid limit characteristics. The aim of this study is to enhance our comprehension of how freeze-thaw cycles can impact the bearing capacity of these soils, thereby providing essential insights for predicting their behavior and potential influence on infrastructure in the context of climate change.

Freeze-thaw effects on pore structure of clay by 3D X-ray computed tomography and mercury intrusion porosimetry

Thaw settlement of soils is a comprehensive reflection of multiscale pore changes induced by freeze-thaw (F-T). In this study, three-dimensional (3D) X-ray computed tomography (CT) tests were utilized to investigate alterations in the macropore and mesopore structures, while mercury intrusion porosimetry (MIP) tests were used to examine the micropore structure in clay due to F-T influenced by different freezing temperatures without water supply. The maximum increase in CT transverse-sectional porosity after F-T can be used to identify where ice lenses formed most abundantly in the clay after thawing, and the diameter and horizontal orientation of the macropores exhibit the most significant increase after thawing. As the freezing temperature decreases, the location becomes farther from the cold end. Macropores are significantly more affected by F-T compared to mesopores, and changes in macropore porosity and diameter can be attributed to moisture migration and freezing shrinkage, with lower freezing temperatures amplifying the influence of freezing shrinkage and weakening the impact of moisture migration. Considering the small size of samples, the MIP porosity was defined to analyze the effects of F-T on the micropores. Compared to the CT volumeratic porosity, the influence of F-T on micropore porosity is less significant. As the freezing temperature decreases, the changes in micropore diameter become smaller. Overall, the lower the freezing temperature, the smaller the changes in macropores, mesopores and micropores. Lastly, a method is proposed for predicting the mass porosity based on the CT volumetric porosity and MIP porosity. This study demonstrates that changes in soil mass porosity reflect a comprehensive representation of multiscale pore variations and provides important theoretical support for thaw settlement control in artificial freezing engineering.

Long-term Freeze–thaw effect on fracture performance of asphalt mixture with reclaimed asphalt pavement, glass fiber and rejuvenator

This investigation explored the influence of reclaimed asphalt pavement (RAP), glass fiber, and rejuvenator on the fracture characteristics of hot mix asphalt (HMA) exposed to freeze–thaw (F–T) conditions. The simulation of long-term F–T action was executed by performing tests across up to 7 F–T cycles. Semi-circular bending (SCB) tests at −10 °C and 25 °C provided insights into the fracture behavior from low to intermediate temperatures. At −10 °C, the incorporation of 25% RAP initially elevated the stress intensity factor without F–T conditioning but adversely affected fracture performance upon sustained F–T exposure. A rejuvenator application resulted in a lower initial stress intensity factor yet improved fracture resistance against long-term F–T action. Conversely, at 25 °C, RAP presence promoted moisture damage accumulation throughout the F–T cycles. A judicious blend of glass fiber and rejuvenator augmented the magnitudes of the J-integral, indicative of better fracture resistance in light of F–T effects. In comparison to H-50R, both 0.05% glass fiber and rejuvenator independently contributed to an increase in fracture energies through all stages of F–T treatment. Nonetheless, their combined impact was more pronounced. The study comprehensively assessed the collective effects of RAP, glass fiber, and rejuvenator on the overall fracture performance, presenting findings that could inform the design of durable asphalt pavements capable of withstanding the rigors of extended F–T exposure.

A novel cement-based interface functional material for application onto shotcrete-rock interface of tunnel in cold regions

In cold regions, the “natural weak zone”+“frost heave cracking” problems at the interface between shotcrete and rock will superimpose damage to the shotcrete-surrounding rock support system structure. However, there is no direct and effective technique to improve the bond strength and frost resistance of shotcrete for infrastructure such as tunnels in cold regions. Based on this, this study innovatively proposes the pretreatment technology of the interface between shotcrete and surrounding rock in tunnels in cold regions, and tries to investigate the fracture evolution of the binary and the distribution characteristics of the interface transition zone (ITZ) in cold regions under the action of interface functional material (IFM) by relying on the techniques of mechanical strength test, morphology analysis, and microstructure test. The study showed that: (1) the optimal mix ratio of IFM was designed as cement (10)+water (4)+calcium acrylate(1.5 %)+bamboo liquefied binder (4 %)+acrylate laminating adhesive (8 %)+ICA (2 %). At room temperature, the shear strength of the binary sample pretreated with the IFM was elevated by 1.56 MPa compared with that of the normal sample, and this increase could be raised to 1.64 MPa under freeze-thaw cycles (FTC). (2) As the freeze-thaw effect intensifies, the number of sandstone particles adhering to the failure surface of the binary increases correspondingly, and the morphology of the failure surface also becomes more undulating. This is due to the fact that after a certain FTC, more cracks are deflected towards the sandstone side due to the higher degradation of the sandstone compared to the ITZ. (3) Under FTC, the application of IFM will lead to an increase in the proportion of sandstone adhering to the failure surface and the fractal dimension of the failure surface, and this phenomenon becomes more pronounced with the intensification of FTC. (4) In the ITZ space, the hydration products and pore structure of the area closer to the sandstone are worse, the use of IFM can optimize this phenomenon. The new idea of utilizing the surrounding rock surface pretreatment technology to improve the bond strength and frost resistance of shotcrete proposed in this study has good application prospects in the construction of tunnels and other infrastructures in cold regions.

Study on meso-deformation and failure mechanism of rock mass with micro-cracks under freeze-thaw loading

The long-term freeze-thaw effect leads to the development of rock fractures and strength degradation in cold region engineering construction, which poses a serious challenge to the stability of the project. In this paper, the microscopic model of sandstone freeze-thaw cycles was established using a particle flow code (PFC2D). Through numerical simulation, the variation law of mechanical properties of rock mass with micro-cracks is systematically studied from the aspects of displacement, crack development, strain and strength. The results show that: (i) The freeze-thaw loading displacement is concentrated on both sides of the initial micro-cracks, and the crack development is not controlled by it. When the number of freeze-thaw cycles is greater than 17, the displacement and crack development are significant. The crack increases with the increase of the crack distance ratio. (ii) When the number of freeze-thaw cycles is less than 15, the compressive crack develops at the end of the initial micro-crack, and the development is controlled by it. When the number of freeze-thaw cycles is greater than 21, the crack increases sharply, and the development is no longer controlled by the initial micro-cracks. When the number of freeze-thaw cycles is less than 15, the damage strain shows minimal variation but decreases sharply with the increase of freeze-thaw cycles. (iii) Under each crack distance ratio, the strength changes in three stages: when the number of freeze-thaw cycles is less than 15, the strength changes little, and the strength decreases sharply with the increase of freeze-thaw cycles. With the increase of the crack distance ratio, it increases first and then decreases. And it is more significant when the number of freeze-thaw cycles is greater than 17. The research results can provide theoretical support for the influence and evaluation of rock freeze-thaw action in cold regions.

Constitutive Characteristics of Rock Damage under Freeze–Thaw Cycles

Freeze–thaw effect is one of the most important environmental conditions that rocks may be subjected to. Through laboratory model tests, the damage characteristics of rocks under the FTC were studied. Based on assuming that the strength of rocks subject to the FTC follows the Weibull distribution, the cumulative damage variable of the number of FTCs was introduced. A cumulative damage constitutive model of shear strength attenuation of rock that meets the Mohr–Coulomb criterion is established. The rationality and applicability of the proposed damage constitutive model are verified by comparing the results of rock shear strength parameters under cyclic freeze–thaw loads.

The Effect of Freeze-thaw on Mechanical and Ultrasonic Properties of Hungarian Oolitic Stones

The freeze-thaw process is a weathering phenomenon that occurs in rocks and stones when water enters small cracks and pores within the stone. When the temperature drops below freezing, the water inside the rock expands as it freezes, exerting pressure on the rock from within. In this study the effect of freeze thaw on mechanical parameter uniaxial compression strength (UCS) and ultrasonic pulse velocity (UPV) of 47 oolitic samples were considered. At first, capillary absorption for cylindrical and prismatic oolitic samples were measured and then freeze-thaw experiment with 23 cycles were applied to the samples. UPV of samples before and after freeze-thaw was analysed by two ultrasonic devices including Geotron and Pundit. It was observed that freeze thaw decreases primary velocity (Vp) and secondary velocity (Vs) by around 20 and 7% respectively. However, average UCS of 1.6 MPa indicates strength decrease of samples, which represent negative effect of pore expansion due to freeze thaw on strength parameter of samples.

Streamlined Analysis of Maternal Plasma Indicates Small Extracellular Vesicles are Significantly Elevated in Early-Onset Preeclampsia

Preeclampsia (PE) is a leading cause of maternal and fetal mortality and morbidity. While placental dysfunction is a core underlying issue, the pathogenesis of this disorder is thought to differ between early-onset (EOPE) and late-onset (LOPE) subtypes. As recent reports suggest that small extracellular vesicles (sEVs) contribute to the development of PE, we have compared systemic sEV concentrations between normotensive, EOPE, and LOPE pregnancies. To circumvent lengthy isolation techniques and intermediate filtration steps, a streamlined approach was developed to evaluate circulating plasma sEVs from maternal plasma. Polymer-based precipitation and purification were used to isolate total systemic circulating maternal sEVs, free from bias toward specific surface marker expression or extensive subpurification. Immediate Nanoparticle Tracking Analysis (NTA) of freshly isolated sEV samples afforded a comprehensive analysis that can be completed within hours, avoiding confounding freeze–thaw effects of particle aggregation and degradation.Rather than exosomal subpopulations, our findings indicate a significant elevation in the total number of circulating maternal sEVs in patients with EOPE. This streamlined approach also preserves sEV-bound protein and microRNA (miRNA) that can be used for potential biomarker analysis. This study is one of the first to demonstrate that maternal plasma sEVs harbor full-length hypoxia inducible factor 1 alpha (HIF-1α) protein, with EOPE sEVs carrying higher levels of HIF-1α compared to control sEVs. The detection of HIF-1α and its direct signaling partner microRNA-210 (miR-210) within systemic maternal sEVs lays the groundwork for identifying how sEV signaling contributes to the development of preeclampsia. When taken together, our quantitative and qualitative results provide compelling evidence to support the translational potential of streamlined sEV analysis for future use in the clinical management of patients with EOPE.

Cyanobacterial Biocrust on Biomineralized Soil Mitigates Freeze–Thaw Effects and Preserves Structure and Ecological Functions

Biocrust inoculation and microbially induced carbonate precipitation (MICP) are tools used in restoring degraded arid lands. It remains unclear whether the ecological functions of the two tools persist when these methods are combined and subjected to freeze–thaw (FT) cycles. We hypothesized a synergetic interaction between MICP treatment and biocrust under FT cycles, which would allow both components to retain their ecological functions. We grew cyanobacterial (Nostoc commune) biocrusts on bare soil and on MICP (Sporosarcina pasteurii)-treated soil, subjecting them to repeated FT cycles simulating the Mongolian climate. Generalized linear modeling revealed that FT cycling did not affect physical structure or related functions but could increase the productivity and reduce the nutrient condition of the crust. The results confirm the high tolerance of MICP-treated soil and biocrust to FT cycling. MICP treatment + biocrust maintained higher total carbohydrate content under FT stress. Our study indicates that biocrust on biomineralized soil has a robust enough structure to endure FT cycling during spring and autumn and to promote restoration of degraded lands.

Orthogonal experimental study on the compressibility characteristics of bank sandy silt based on freeze-thaw effects

The compressibility of bank soils is a critical consideration in t riverbank protection project, construction. In the Inner Mongolia section of the Yellow River, a seasonal frozen soil region, the impact of F-T cycles on soil compressibility cannot be overlooked. This study, based on F-T cycle tests, conducted orthogonal consolidation tests on embankment sandy silt from the Inner Mongolia section of the Yellow River, considering varying initial w and ρd. It investigated the effects of different initial w, ρd, and F-T cycles on soil compressibility, ranked and assessed the significance of influencing factors, and established a predictive model for the compressibility of Inner Mongolia section Yellow River embankment sandy silt. The results show that: the embankment sandy silt is medium-high compression soil, with a between 0.1 and 0.74 MPa−1, Es between 2.65 and 18.47 MPa, and Cc between 0.04 and 0.24 MPa. The greater the ρd of soil, the smaller the initial w, the smaller the a and Cc, and the greater the Es of soil. The F-T effect affects soil compressibility, and soil a, Es and Cc are linearly related to the number of F-T cycles. The ranking of factors influencing soil compressibility is ρd > w > F-T cycles. The initial ρd and w are decisive factors affecting soil compressibility, while the impact of F-T cycles is relatively minor. Additionally, a regression predictive model based on the initial ρd and w of the soil demonstrates good performance in predicting soil compression indices. This model can be utilized for predicting the compressibility indices of embankment soil in the Inner Mongolia section of the Yellow River.

Effects of freeze–thaw on bank soil mechanical properties and bank stability

Riverbank instability in the seasonally frozen zone is primarily caused by freeze–thaw erosion. Using the triaxial freeze–thaw test on the bank of Shisifenzi Bend in the Yellow River section of Inner Mongolia, we investigated the changes in the mechanical properties of the soil at different freezing temperatures and freeze–thaw times, and analyzed the bank’s stability before and after freezing based on the finite element strength reduction method. The results showed that the elastic modulus, cohesion, internal friction angle and shear strength of the soil tended to decrease with the increase in the number of freeze–thaw cycles and the decrease in freezing temperature. After 10 freezing cycles at − 5 ℃, − 10 ℃, − 15 ℃ and − 20 ℃, the modulus of elasticity of soil decreased by 40.84 ~ 68.70%, the cohesion decreased by 41.96 ~ 56.66%, the shear strength decreased by 41.92 ~ 57.32%, respectively. Moreover, the stability safety coefficient of bank slope decreased by 18.58% after freeze–thaw, indicating that the freeze–thaw effect will significantly reduce the stability of bank slope, and the bank slope is more likely to be destabilized and damaged after freeze–thaw.

Analysis of the impact of pluronic acid on the thermal stability and infectivity of AAV6.2FF

BackgroundThe advancement of AAV vectors into clinical testing has accelerated rapidly over the past two decades. While many of the AAV vectors being utilized in clinical trials are derived from natural serotypes, engineered serotypes are progressing toward clinical translation due to their enhanced tissue tropism and immune evasive properties. However, novel AAV vectors require formulation and stability testing to determine optimal storage conditions prior to their use in a clinical setting.ResultsHere, we evaluated the thermal stability of AAV6.2FF, a rationally engineered capsid with strong tropism for lung and muscle, in two different buffer formulations; phosphate buffered saline (PBS), or PBS supplemented with 0.001% non-ionic surfactant Pluronic F68 (PF-68). Aliquots of AAV6.2FF vector encoding the firefly luciferase reporter gene (AAV6.2FF-ffLuc) were incubated at temperatures ranging from -20°C to 55°C for varying periods of time and the impact on infectivity and particle integrity evaluated. Additionally, the impact of several rounds of freeze-thaw treatments on the infectivity of AAV6.2FF was investigated. Vector infectivity was measured by quantifying firefly luciferase expression in HEK 293 cells and AAV particle integrity was measured by qPCR quantification of encapsidated viral DNA.ConclusionsOur data demonstrate that formulating AAV6.2FF in PBS containing 0.001% PF-68 leads to increased stability and particle integrity at temperatures between -20℃ to 21℃ and protection against the destructive effects of freeze-thaw. Finally, AAV6.2FF-GFP formulated in PBS supplemented with 0.001% PF-68 displayed higher transduction efficiency in vivo in murine lung epithelial cells following intranasal administration than vector buffered in PBS alone further demonstrating the beneficial properties of PF-68.