Freeze-dried Cabbage Research Articles

Simultaneous Determination of Neonicotinoid and Carbamate Pesticides in Freeze-Dried Cabbage by Modified QuEChERS and Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry.

Dehydrated vegetables are popular in instant foods, but few reports have focused on their pesticide residues. This research developed and validated a modified QuEChERS method combined with ultra-performance liquid chromatography-tandem mass spectrometry to determine 19 kinds of neonicotinoid and carbamate pesticides in freeze-dried cabbage. Herein, acetonitrile/water (v/v = 2:1) was selected in the extraction step. Meanwhile, 4 g anhydrous magnesium sulfate and 1 g sodium chloride were applied to the partitioning step. Dispersive solid-phase extraction sorbents were selected, and liquid chromatography conditions were further optimized for dealing with the matrix effect. The limits of quantification ranged from 1.0 to 10.0 μg/kg. The validation results were acceptable, with average recoveries of 78.7-114.0% and relative standard deviations below 14.2%. The method recoveries were closely related to the volume proportion of water in the extractant. Finally, the developed method was applied to real freeze-dried cabbages and four pesticides (propamocarb, imidacloprid, acetamiprid, and thiacloprid) were detected in six samples.

Antiatherogenic effects of Korean cabbage kimchi with added short arm octopus

Adimchae is a kimchi that contains more than 10% seafood. The lipid lowering and antiatherogenic effects of adimchae containing abalone or short arm octopus were compared with traditional Korean cabbage kimchi. Treated seafood was added to kimchi at 10%. ApoE KO mice (n=18) were fed an athrogenic diet supplemented with 10% freeze-dried cabbage kimchi (control), short arm octopus added kimchi (SK), or abalone added kimchi (AK) for 8 weeks. Plasma and hepatic triglyceride concentrations of SK group mice decreased significantly (p<0.05), compared with control and AK group mice. Protein expression of hepatic fatty acid synthase (FAS) of SK group mice was significantly (p<0.05) suppressed, compared with control group mice. Reduction of the fatty streak lesion size in the aortic sinus of SK mice was also significant (p<0.05), compared with control group mice. Extra antiatherogenic effects of SK were due to decreased plasma lipid concentrations via suppression of the FAS activity.

Estrogenic Effects of Extracts from Cabbage, Fermented Cabbage, and Acidified Brussels Sprouts on Growth and Gene Expression of Estrogen-Dependent Human Breast Cancer (MCF-7) Cells

Cruciferous vegetable extracts from freeze-dried cabbage (FDC), freeze-dried fermented cabbage (FDS), and acidified Brussels sprouts (ABS) were prepared by exhaustive extraction with ethyl acetate. Estrogenic and antiestrogenic effects of these extracts were analyzed. To identify whether the extracts are potential estrogen receptor (ER) ligands that can act as agonists or antagonists, the binding affinity of extracts for the ER was measured using a competitive radiometric binding assay. The extracts bound with low affinity to the ER, and the relative binding affinity is estradiol > FDS > FDC > ABS. These extracts were evaluated for their estrogenic and antiestrogenic activities in estrogen-dependent human breast cancer (MCF-7) cells using as endpoints proliferation and induction of estrogen-responsive pS2 gene expression, which was analyzed using Northern blot assay. At low concentrations (5-25 ng/mL) all of the extracts reduced 1 nM estradiol-induced MCF-7 cell proliferation. Extracts at 25 ng/mL also inhibited estradiol-induced pS2 mRNA expression. At higher extract concentrations (50 ng/mL-25 microg/mL), however, increased proliferation in MCF-7 cells was observed. Similarly, expression of the pS2 gene was induced by higher extract concentrations (0.25-25 microg/mL). The pure estrogen antagonist, ICI 182,780, suppressed the cell proliferation induced by the extracts as well as by estradiol and also the induction of pS2 expression by the extracts. The ER subtype-selective activities of FDC and FDS were analyzed using a transfection assay in human endometrial adenocarcinoma (HEC-1) cells. FDS acted as an ERalpha-selective agonist while FDC fully activated both ER-alpha and ER-beta. Growth of the ER-negative MDA-231 cells was not affected by the extracts or by estradiol. This study demonstrates that cruciferous vegetable extracts act bifunctionally, like an antiestrogen at low concentrations and an estrogen agonist at high concentrations.

Chemoprevention of 2-amino-1-methyl-6-phenyli-midazo 4,5-b pyridine-induced carcinogen-DNA adducts by Chinese cabbage in rats.

AIM:The food-borne carcinogen 2-amino-1-methyl-6-phenylimidazo 4,5-b pyridine (PhIP) induces colon and mammary gland tumors in rats and has been implicated in the etiology of human colorectal cancer.This study was conducted to examine the potentially preventive effect of Chinese cabbage (Brassica chinensis),a brassica vegetable most commonly consumed in China, against this carcinogen-induced DNA adduct formation in rats and its possible mechanisms.METHODS:Sprague-Dawley rats were maintained for 10 days on basal diet or diet containing 20% (w/w) freeze-dried cabbage powder prior to administration of a single dose of PhIP (10mg/kg) by oral gavage. Rats were sacrificed at 20h after PhIP treatment and PhIP-DNA adducts in the colon, heart, lung and liver were analyzed using (32)P-postlabeling technique. Levels of hepatic cytochrome P450 (CYP)1A1 and 1A2, as indicated by 7-ethoxyresorufin O-deethylase and 7-methoxyresorufin O-demethylase activity, and cytosolic glutathione S-transferases (GSTs) towards 1-chloro-2, 4-dinitrobenzene (CDNB) in the liver, lung and colon were measured.RESULTS:Rats pre-treated with Chinese cabbage and given a single dose of PhIP had reduced levels of PhIP-DNA adducts in the colon, heart, lung and liver, with inhibition rates of 82.3%, 60.6%, 48.4% and 48.9%, respectively (P< 0.01). The enzyme assays revealed that Chinese cabbage induced both CYP1A1 and 1A2 activity, but the induction was preferential for CYP1A1 over 1A2 (81% vs 51%).GST activity towards CDNB in the liver and lung, but not colon, was also significantly increased by cabbage treatment.CONCLUSION:The results indicate that Chinese cabbage has a preventive effect on PhIP-initiated carcinogenesis in rats and the mechanism is likely to involve the induction of detoxification enzymes.

A technique for assessing the biological availability of folate in foods

A method is described for the assessment of folate availability from human foods using the the folate-depleted rat. Animals were maintained on a semisynthetic diet for periods of 4 weeks or more. At the end of this period tissue folate levels were measured using a radioimmunoassay. Folate intakes were assessed by monitoring food intake on a daily basis. The folate content of the diet was determined by microbiological assay. The animals continued to grow and maintain health throughout the depletion period, but levels of folate in liver, serum and intestinal mucosa were significantly reduced in comparison to rats fed a folate supplemented diet. Refeeding depleted rats with crystalline folic acid, or with freeze-dried cabbage incorporated into the semi-synthetic diet led to a measurable increase in tissue folate stores within 24 h. The serumfolate level appeared to be the most consistent and conveniently measurable index of folate status. The method provides a relatively inexpensive and rapid method of measuring the physiological response to the most important sources of folate in the human diet.

The effects of dietary cabbage on xenobiotic-metabolizing enzymes and the binding of aflatoxin B 1 to hepatic DNA in rats

The effect of dietary cabbage ( Brassica oleracea) on the binding of aflatoxin B 1 (AFB 1) to hepatic DNA and on the activities of liver and intestinal microsomal and cytosolic enzymes was studied in weanling male Fischer 344 rats. Freeze-dried cabbage was fed to rats at a level of 25% in the diet for 21 days, while others received a basal diet. In the cabbage-fed group there was an 87% ( P < 0.01) reduction in the binding of AFB 1 to hepatic DNA 2 hr after the ip injection of [ 3H]AFB 1 (3 μg/kg). There was also a 41% ( P < 0.05) increase in liver weight expressed relative to body weight. Hepatic and intestinal glutathione S-transferase activities were significantly increased (2.1- and 2.3-fold, respectively) over those in rats fed the basal diet. Hepatic and intestinal microsomal epoxide hydrolase activities were significantly increased (2.6- and 1.4-fold, respectively) over the basal group. Intestinal aryl hydrocarbon hydroxylase (AHH) and ethoxycoumarin O-deethylase (ECD) activities were significantly increased (2.3- and 2.5-fold, respectively), over the basal group but dietary cabbage had no significant effect on hepatic AHH or ECD activities.

Nonenzymic discoloration in dried cabbage. II. Red condensation product of dehydroascorbic acid and glycine ethyl ester

The cherry red colour formed initially in the browning of freeze-dried cabbage has been studied using a model system consisting of dehydroascorbic acid (DHA) and glycine ethyl ester which produces a chromogen of similar colour characteristics. Elemental analysis, gel elution, and spectral studies indicated that the reaction takes place in equimolar proportions to give a dimeric, unsaturated condensation product, the probable structure of which is discussed. The chromogen is labile, hygroscopic, unstable in aqueous or acidic medium, and is light and heat sensitive. [See Journal of Agricultural and Food Chemistry (1968) 16, 529 for part I.]