Free Trans Research Articles

Use of Tension free Trans Obturator Tape in treatment of Stress Urinary Incontinence: a Recent Gold Standard Approach

Dr. Sweety Bhuriya 1 , Dr. Vyas Rupa C. 2-A , Dr. Shah Sapana R. 2-B , Dr. Mona Gamit 2-C , (Resident Doctor, Department of Obstetrics and Gynecology Smt. N.H.L. Municipal medical college and Sheth V.S. General Hospital) (Assistant professor, Department of Obstetrics and Gynecology, Smt. N.H.L. Municipal medical college and Sheth V.S. General Hospital) (Associate professor and head of unit, Department of Obstetrics and gynecology, Smt. N.H.L. Municipal medical college and Sheth V.S. General Hospital) (Resident Doctor, Department of Obstetrics and Gynecology, Smt. N.H.L. Municipal medical college and Sheth V.S. General Hospital)

Separation of cis-trans phospholipid isomers using reversed phase LC with high resolution MS detection.

The increased presence of synthetic trans fatty acids into western diets has been shown to have deleterious effects on physiology and raising an individual's risk of developing metabolic disease, cardiovascular disease, and stroke. The importance of these fatty acids for health and the diversity of their (patho) physiological effects suggest that not only should the free trans fatty acids be studied but also monitoring the presence of these fats into the side chains of biological lipids, such as glycerophospholipids, is also essential. We developed a high resolution LC-MS method that quantitatively monitors the major lipid classes found in biospecimens in an efficient, sensitive, and robust manner while also characterizing individual lipid side chains through the use of high energy collisional dissociation (HCD) fragmentation and chromatographic alignment. We herein show how this previously described reversed phase method can baseline separate the cis-trans isomers of phosphatidylglycerol and phosphatidylcholine (PC) with two 18:1 side chains, in both positive and negative mode, as neat solutions and when spiked into a biological matrix. Endogenous PC (18:1/18:1)-cis and PC (18:1/18:1)-trans isomers were examined in mitochondrial and serum profiling studies, where rats were fed diets enriched in either trans 18:1 fatty acids or cis 18:1 fatty acids. In this study, we determined the cis:trans isomer ratios of PC (18:1/18:1) and related this ratio to dietary composition. This generalized LC-MS method enables the monitoring of trans fats in biological lipids in the context of a nontargeted method, allowing for relative quantitation and enhanced identification of unknown lipids in complex matrixes.

899: Tension Free Vaginal Tape vs Trans Obturator Tape as Surgery for Stress Urinary Incontinence: Results of a Multicentre Randomised Trial

899: Tension Free Vaginal Tape vs Trans Obturator Tape as Surgery for Stress Urinary Incontinence: Results of a Multicentre Randomised Trial

Synthesis and molecular structures of dinuclear complexes with 1,2-dihydro-1,2-diphenyl-naphtho[1,8-c,d]1,2-diphosphole as a bridging ligand

A bisphosphine in which a PhP–PPh bond bridges 1,8-positions of naphthalene, 1,2-dihydro-1,2-diphenyl-naphtho[1,8-cd]-1,2-diphosphole ( 1), was used as a bridging ligand for the preparation of dinuclear group 6 metal complexes. Free trans- 1, a more stable isomer having two phenyl groups on phosphorus centers mutually trans with respect to a naphthalene plane, was allowed to react with two equivalents of M(CO) 5(thf) (M = W, Mo, Cr) at room temperature to give dinuclear complexes (OC) 5M(μ- trans- 1)M(CO) 5 (M = W ( 2a), Mo ( 2b), Cr ( 2c)). The preparation of the corresponding dinuclear complexes bridged by the cis isomer of 1 was also carried out starting from the free trans- 1 in the following way. Mono-nuclear complexes M( trans- 1)(CO) 5 (M = W ( 3a), Mo ( 3b), Cr ( 3c)) which had been prepared by a reaction of trans- 1 with one equivalent of the corresponding M(CO) 5(thf) (M = W, Mo, Cr) complex, were heated in toluene, wherein a part of the trans- 3a– c was converted to their respective cis isomer M( cis- 1)(CO) 5. Each cis trans mixture of the mono-nuclear complexes 3a– c was treated with the corresponding M(CO) 5(thf) to give a cis trans mixture of the respective dinuclear complexes 2a– c. The cis isomer of the ditungsten complex 2a was isolated, and its molecular structure was confirmed by X-ray analysis, showing a shorter W⋯W distance of 5.1661(3) Å than that of 5.8317(2) Å in trans- 2a.

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The significance of high renal uptake found in patients with hematological disease who underwent bone marrow scintigraphy with 111In-chloride was investigated. In patients with a high renal uptake, the uptake in bone marrow was low, suggesting a reflection of erythropoietic activity. The reversal relationship, however, was not necessarily present. With regard to correlation with iron metabolism, significantly higher uptake of serum Fe and lower UIBC were found among those with high renal uptake, suggesting that the level of free trans ferrin in the serum is largely involved in the high uptake in the kidney.

Structure-Activity Relationships of Retinoids in the Morphogenesis of Cutaneous Appendages in the Chick Embryo

We have evaluated the efficacy of 13 analogues of retinoic acid (retinoids) in producing in vivo feathers on the normally scaly foot integument (ptilopody) of the chick embryo. The retinoids were dissolved in dimethylsulfoxide and injected at doses from 1.25-250 micrograms into the amniotic cavity of embryos of 10 days of incubation. The results showed that the production of ptilopody requires specific retinoids, namely, those incorporating a free trans carboxylic end group. Indeed, the esterification of this acid end group (retinoid ethyl ester) leads to a delay in the effect of the product. Its substitution by an alcohol (retinol) or an aldehyde group (retinal), or its isomerization, leads to inactive products. The esterification by acetic acid of the alcohol terminal group of retinol leads to a moderately active compound (retinyl acetate). By contrast, modifications of the ring do not interfere with the efficacy of the retinoid, and modifications of the side chain by addition of one or two supplementary rings lead to an increase of the activity.

Time Course of the Metabolism of Abscisic Acid and its trans-trans Isomer in Cell Suspension Cultures of Lycopersicon peruvianum

Summary The time course of the metabolization of abscisic acid (ABA) and its trans-trans isomer (trans-ABA) by cell suspension cultures of Lycopersicon peruvianum was investigated during 20 min and 5 h incubation. Whereas ABA is biotransformed by the cells both to acidic metabolites and to glucosyl ester, trans -ABA is converted only to the glucose conjugate. The conjugation of trans -ABA is faster than that of ABA; after 5 h no free trans -ABA was observed but free ABA in relative high concentration. The glucosyl esters of ABA and trans -ABA were accumulated by the cells without transport to the culture medium. The conjugates seems to be stable for at least 5 h.

Platinum(II) and palladium(II) complexes of thiacyclooct-4-enes; an unusual metal-catalyzed olefin isomerization

Reactions of MCl 4 2− (M = Pd, Pt) with cis-thiacyclooct-4-ene, cis-2-methylthiacyclooct-4-ene and cis-2-phenylthiacyclooct-4-ene ( cis-S-4-oct) give chelate complexes of general formula MCl 2( cis-S-4-oct). X-ray structure determination indicates that coordination to the metal occurs through both the olefinic double bond and the sulfur atom. Reaction of CN − with the adduct obtained from PtCl 4 2− and a mixture of cis- and trans-2-methylthiacyclooct-4-ene liberates the cis ligand, and GLC analysis excludes the presence of free trans olefin. It is suggested that platinum complexes induce extensive trans→cis olefin isomerization.

Co-ordination stabilization of reactive molecules: preparation of trans-[(en)2Co(OH2)(S3O3)]+, a cobalt(III) complex containing disulphane monosulphonate

cis-[(en)2Co(S2O3)2]– is oxidized by aqueous iodine to yield free sulphate and trans-[(en)2Co(OH2)(S3O3)]+, a moderately stable complex containing the very reactive ligand, disulphane monosulphonate.

X-ray crystal structure of azoxybenzene oxotetrachlorotungsten(VI), (C 6H 5N) 2OW VIOCl 4

The crystal and molecular structure of azoxybenzene oxotetrachlorotungsten(VI) has been determined from X-ray diffractometer data. The structure was solved by Patterson and Fourier methods and refined by least-squares techniques to R = 0.058 for 1869 independent reflections. The crystals are monoclinic, space group P2 1/ c, with Z = 4, in a unit cell of dimensions: a = 8.314(3), b = 15.100(5), c = 12.901(7) Å, α = 95.31(5)°. The azoxybenzene residue, the structure of which resembles that of free trans azobenzene, is linked to the tungsten atom through its oxygen atom. The coordination at the metal (two oxygen atoms and four chlorine atoms) corresponds to a distorted octahedron. This distortion is very similar to those observed in similar tungsten compounds. There is a intramolecular C⋯O distance of 2.77 Å between two atoms four bonds apart, of the azoxybenzene residue.