Free-ranging Sheep Research Articles

Habitat Selection by Free-Grazing Sheep in a Mountain Pasture.

For centuries, mountain grasslands have been part of the grazing cycle of sheep and cattle in extensive management systems in the Pyrenees; however, traditional guided herding has been, in many cases, replaced by free-ranging flocks at these pastures. The goal of this study was to analyze the grazing behavior of free-ranging sheep in mountain pastures using GPS-based tracking systems and Geographic Information Systems. During the summer seasons of 2019, 2020, and 2021, a transterminant flock of free-ranging sheep was tracked with GPS devices attached to collars at the mountain pastures of Collarada mountain (Central Pyrenees, Spain). Preferences for grazing on certain geomorphological features (slope, aspect, and altitude) and different plant communities present in the area were evaluated using the Manly's standardized preference index. The results show that sheep prefer altitudes between 2400 and 2600 m; SE-, SW-, and E-facing sites; and slopes under 20%. The preferred pastures were Festucion eskiae, Primulion intricatae, and Nardion strictae; however, they reject Festucion scopariae communities. This study demonstrates the effectiveness of GPS and GIS technologies in monitoring free-ranging sheep activity, providing valuable data for enhancing pastoral practices and ensuring sustainable pasture use.

Cryptosporidium spp. in large-scale sheep farms in China: prevalence and genetic diversity

Cryptosporidium spp. are significant zoonotic intestinal parasites that induce diarrhea and even death across most vertebrates, including humans. Previous studies showed that sheep are important hosts for Cryptosporidium and that its distribution in sheep is influenced by geography, feeding patterns, age, and season. Environmental factors also influence the transmission of Cryptosporidium. Molecular studies of Cryptosporidium in sheep have been conducted in only a few regions of China, and studies into the effect of sheep-housing environments on Cryptosporidium transmission are even rarer. To detect the prevalence of Cryptosporidium in large-scale sheep-housing farms, a total of 1241 fecal samples were collected from sheep, 727 environmental samples were taken from sheep housing, and 30 water samples were collected in six regions of China. To ascertain the existence of the parasite and identify the species of Cryptosporidium spp., we conducted nested PCR amplification of DNA extracted from all samples using the small-subunit (SSU) rRNA gene as a target. For a more in-depth analysis of Cryptosporidium spp. subtypes, C. xiaoi-and C. ubiquitum-positive samples underwent separate nested PCR amplification targeting the 60 kDa glycoprotein (gp60) gene. The amplification of the Cryptosporidium spp. SSU rRNA gene locus from the whole genomic DNA of all samples yielded a positive rate of 1.2% (20/1241) in fecal samples, 0.1% (1/727) in environmental samples, and no positive samples were found in water samples. The prevalence of Cryptosporidium spp. infection in large-scale housed sheep was 1.7%, which was higher than that in free-ranging sheep (0.0%). The highest prevalence of infection was found in weaning lambs (6.8%). Among the different seasons, the peaks were found in the fall and winter. The most prevalent species were C. xiaoi and C. ubiquitum, with the former accounting for the majority of infections. The distribution of C. xiaoi subtypes was diverse, with XXIIIc (n = 1), XXIIId (n = 2), XXIIIe (n = 2), and XXIIIl (n = 4) identified. In contrast, only one subtype, XIIa (n = 9), was found in C. ubiquitum. In this study, C. xiaoi and C. ubiquitum were found to be the predominant species, and Cryptosporidium was found to be present in the environment. These findings provide an important foundation for the comprehensive prevention and management of Cryptosporidium in intensively reared sheep. Furthermore, by elucidating the prevalence of Cryptosporidium in sheep and its potential role in environmental transmission, this study deepens our understanding of the intricate interactions between animal health, environmental contamination, and public health dynamics.

Heat stress conditions affect the social network structure of free-ranging sheep.

Extreme weather conditions, like heatwave events, are becoming more frequent with climate change. Animals often modify their behaviour to cope with environmental changes and extremes. During heat stress conditions, individuals change their spatial behaviour and increase the use of shaded areas to assist with thermoregulation. Here, we suggest that for social species, these behavioural changes and ambient conditions have the potential to influence an individual's position in its social network, and the social network structure as a whole. We investigated whether heat stress conditions (quantified through the temperature humidity index) and the resulting use of shaded areas, influence the social network structure and an individual's connectivity in it. We studied this in free-ranging sheep in the arid zone of Australia, GPS-tracking all 48 individuals in a flock. When heat stress conditions worsened, individuals spent more time in the shade and the network was more connected (higher density) and less structured (lower modularity). Furthermore, we then identified the behavioural change that drove the altered network structure and showed that an individual's shade use behaviour affected its social connectivity. Interestingly, individuals with intermediate shade use were most strongly connected (degree, strength, betweenness), indicating their importance for the connectivity of the social network during heat stress conditions. Heat stress conditions, which are predicted to increase in severity and frequency due to climate change, influence resource use within the ecological environment. Importantly, our study shows that these heat stress conditions also affect the animal's social environment through the changed social network structure. Ultimately, this could have further flow on effects for social foraging and individual health since social structure drives information and disease transmission.

Effect of Different Feeds on the Fungi Microbiome of Suffolk Crossed with Tibetan Sheep.

The gut microbiome plays an important role in the metabolism, nutrient absorption and immunocompetency of animals. The dynamics of the microbiota can be influenced by modulatory factors that involve nutrition, environment, health, diseases, etc. Few reports have been documented regarding the effects of different feeds on the fungi microbiome of Suffolk crossed with Tibetan sheep. A total of 30 Suffolk crossed with Tibetan sheep (ST sheep) were selected for the study and randomly divided into five equal groups (n = 6): AZ, BZ, CZ, DZ and EZ. Group AZ was fed with alfalfa and oat grass, whereas group BZ was fed with mixture of concentrated feed, alfalfa and oat grass. Groups CZ, DZ and EZ were fed with concentrated feed #1, #2 and #3, respectively. All experimental animals were fed twice a day for four months, and rectum samples were collected for microbiota analysis. Results revealed that 2,781,461 raw reads and 2,333,239 clean reads were achieved in the ST sheep. When compared with the sheep of groups AZ and BZ (164), the shared amplicon sequence variants (ASVs) between AZ and CZ (109), AZ (113) and DZ (118) as well as AZ along with EZ were fewer. Conspicuous different phyla (8) and genera (56) were examined and compared with free-range sheep in AZ. Genera including Xeromyces, Kazachstania, Cordyceps, Rhodotorula, Pichia, Spor, etc. were found higher in animals in the CZ, DZ and EZ groups. The results of this study provide new insights regarding the effects of different feeds on the fungi microbiome of sheep farmed on the plateau. We concluded that the differences in feed in Suffolk crossed with Tibetan sheep altered their gut microbiota.

Tracking free-ranging sheep to evaluate interrelations between selective grazing, movement patterns and the botanical composition of alpine summer pastures in northern Italy

Extensive sheep pasturing in alpine regions has a long tradition and fulfils numerous sociological, economic and ecological functions. The effects of sheep grazing on the floristic composition and vice versa depend on various factors. Knowledge of potential interrelations is crucial to developing adequate management systems to maintain pasture productivity and its unique biodiversity. The aim of the present study was to discuss the potential interrelations between movement and selective grazing behaviour of free-ranging unherded sheep and the botanical composition of high-altitude mountain pastures in northern Italy. General movement patterns were determined by using GPS tracking. The floristic composition of areas roamed by the sheep was analysed by collecting physical data during the summer of 2022. The energy content of ingested herbage biomass was determined based on faecal samples. Ranging between 2296 and 3015 m above sea level (a.s.l.), the average altitude used by the sheep was 2654 m a.s.l. Correlation analyses showed that the sheep used significantly higher altitudes with increasing temperature and sunshine duration and with decreasing air humidity and rainfall. A clear selective grazing behaviour was revealed, namely a preference for species with better nutritional attributes. Poa alpina was the most preferred species, while areas dominated by Nardus stricta were avoided. Furthermore, the sheep showed an uphill migration over the season, possibly caused by the delayed start of grassland growth at higher altitudes. Analyses of faecal samples revealed sufficient energy contents, presumably as a result of the targeted selection of nutritious plant species. Future studies should evaluate the feeding value of herbage on offer in order to validate the current results. The study highlights the opportunity of animal tracking in remote areas and provides indications for selective grazing of sheep under conditions of free choice.

Fission-fusion dynamics in sheep: the influence of resource distribution and temporal activity patterns.

Fission-fusion events, i.e. changes to the size and composition of animal social groups, are a mechanism to adjust the social environment in response to short-term changes in the cost-benefit ratio of group living. Furthermore, the time and location of fission-fusion events provide insight into the underlying drivers of these dynamics. Here, we describe a method for identifying group membership over time and for extracting fission-fusion events from animal tracking data. We applied this method to high-resolution GPS data of free-ranging sheep (Ovis aries). Group size was highest during times when sheep typically rest (midday and at night), and when anti-predator benefits of grouping are high while costs of competition are low. Consistent with this, fission and fusion frequencies were highest during early morning and late evening, suggesting that social restructuring occurs during periods of high activity. However, fission and fusion events were not more frequent near food patches and water resources when adjusted for overall space use. This suggests a limited role of resource competition. Our results elucidate the dynamics of grouping in response to social and ecological drivers, and we provide a tool for investigating these dynamics in other species.

Molecular detection of Rickettsia, Anaplasma, and Bartonella in ticks from free-ranging sheep in Gansu Province, China

Ticks pose a serious threat to public health as carriers and often vectors of zoonotic pathogens. There are few systematic studies on the prevalence and genetic diversity of tick-borne bacterial pathogens in Western China. In this study, 465 ticks were collected from free-ranging sheep in Gansu Province in China. Ticks were divided into 113 pools and tick DNA was extracted from these ticks. PCR assays were performed using specific primers to screen for tick-borne pathogens as well as sequence analysis based on the 16S rRNA (rrs), ompB, gltA, ompA genes for Rickettsia, rrs, groEL genes for Anaplasma, and ssrA and rpoB genes for Bartonella. The PCR results showed that the minimum infection rates with Rickettsia, Anaplasma, and Bartonella were 16.8% (78/465), 18.9% (88/465), and 0.9% (4/465), respectively. Sequence analysis based on the concatenated sequences of rrs-ompB-gltA-ompA indicated that the Rickettsia species identified in the ticks belonged to Rickettsia raoultii, Rickettsia slovaca, and Rickettsia sibirica, respectively; phylogenetic analysis based on the groEL gene showed that all Anaplasma strains identified were Anaplasma ovis; and phylogenetic analysis based on the ssrA and rpoB genes indicated that all Bartonella strains in the ticks belonged to Bartonella melophagi. The results of this study showed that ticks in Gansu Province harbored multiple pathogens that may cause rickettsial diseases and bartonellosis. These diseases were neglected in the area and physicians and public health workers need to pay attention to their diagnoses to prevent human infection.

Comparison of Antimicrobial Resistance, Virulence Genes, Phylogroups, and Biofilm Formation of Escherichia coli Isolated From Intensive Farming and Free-Range Sheep.

Pathogenic E. coli are among the most frequently isolated bacterial pathogens on large-scale sheep farms in China. Antibiotic use in wool sheep production is a risk factor for promoting the emergence of resistant E. coli. To reveal the differences of E. coli populations in sheep from different farming systems the antimicrobial resistance, virulence genes, biofilm formation, and phylogroups of 500 E. coli isolates obtained between September 2019 and December 2020 in northwest China from diarrheic infections of intensive farming and free-range sheep were analyzed. The antimicrobial susceptibility test for 12 classes of antimicrobial agents was determined using the broth microdilution susceptibility method, and PCR was used to detect the differences in virulence genes and phylogroups. Additionally, biofilm formation was determined using microtiter plate and slide agglutination methods. Among the 500 E. coli isolates, the majority of the isolates were multidrug resistant (75.4%) and carried at least one virulence gene (94.8%). We observed that 412 (82.4%), 360 (72.0%), and 266 (53.2%) are found to be resistant to sulfisoxazole, florfenicol, and tetracyclines, respectively. Resistance was also observed to mequindox (46.8%), ampicillin (43.6%), spectinomycin (38.6%), enrofloxacin (34.2%), ceftiofur (21.0%), gentamycin (20.4%), ceftazidime (17.8%), and polymyxin B (7.8%) but no resistance was found to meropenem. These results showed that strains from free-range subjects had fewer antibiotic resistance strains rather than sheep that were intensively farmed (P < 0.05). We observed fifteen virulence genes, of which etrA (n = 401, 80.2%) is the most common. In addition, EAEC (86.4%) is dominant among free-range sheep and EHEC (80.1%) is dominant among intensive farming. Among all virulence genes, the strongest correlation was found between etrA and papC gene (P < 0.001, OR = 455.68). Similarly, the strongest correlation was also found between eltA and sulfisoxazole (P < 0.001, OR = 877). Furthermore, the majority of the E. coli isolates belonged to phylogroup B1 (50.6%), followed by phylogroup C (20.6%), A (7.4%), E (7.4%), D (5.8%), B2 (1.6%), and F (1%). Interestingly, phylogroup B2 and D were all distributed in intensive farms. In addition, 33 (6.6%), 373 (74.6%), and 94 (18.8%) showed moderate, weak, and no connection biofilm formation ability, respectively. These data uncovered that wool sheep serve as a reservoir of pathogenic E. coli harboring multiple resistance phenotypes and virulence genes. The overlapping virulence-associated traits between IPEC and ExPEC indicated the zoonotic potential and safety threats of sheep food products. It is urgent to improve the proper use of antimicrobials in China as well as other countries.

Breed effects and heterosis for weight traits and tick count in a cross between an indigenous fat-tailed and a commercial sheep breed.

Ticks can compromise productivity and welfare in free-ranging sheep. Chemical tick control may not be sustainable in the long term. Alternative control measures must be sought for an integrated control programme. Birth and weaning weights as well as log transformed overall tick count of indigenous fat-tailed Namaqua Afrikaner (NA), commercial Dorper and NA x Dorper cross lambs were studied under extensive conditions. Relative to NA lambs, Dorper lambs were 22.2% heavier at weaning (P < 0.05). Geometric means for total tick count on Dorper lambs exceeded those of their Namaqua Afrikaner contemporaries by more than twofold (P < 0.05). Relative to the pure-breed midparent value, the mean performance of NA x Dorper lambs was 7.9% more for birth weight, 11.2% more for weaning weight and 26.2% less for the back transformed means for total tick count (P < 0.05). Heterosis for total tick count was slightly greater at -29.3% when data were adjusted for the larger size of NA x Dorper lambs. Crossing commercial Dorper sheep with a hardy, indigenous breed therefore resulted in lower levels of tick infestation without compromising live weight in progeny so derived. Hardy, indigenous genetic resources like the NA should be conserved and used in further studies of ovine genetics of resistance to ticks in South Africa.

Assessing the role of livestock and sympatric wild ruminants in spreading antimicrobial resistant Campylobacter and Salmonella in alpine ecosystems

BackgroundLivestock play an important role as reservoir of enteric pathogens and antimicrobial resistance (AMR), a health and economic concern worldwide. However, little is known regarding the transmission and maintenance of these pathogens at the wildlife-livestock interface. In this study, we assessed the occurrence, genetic diversity and AMR of Campylobacter spp. and Salmonella spp. shed by sympatric free-ranging livestock and a wild herbivore in an alpine ecosystem.ResultsCampylobacter spp. was isolated from 23.3 % of cattle and 7.7 % of sheep but was not isolated from horses nor Pyrenean chamois (Rupicapra pyrenaica). Campylobacter jejuni was the most frequent species. A high genetic diversity and certain host specificity of C. jejuni isolates was observed. The main AMR detected in Campylobacter isolates was to nalidixic acid (88.2 %), ciprofloxacin (82.4 %) and tetracycline (82.4 %); only 11.7 % of the isolates were pan-susceptible and 17.6 % were multi-resistant. Salmonella ser. Newport was isolated only from one Pyrenean chamois and was pan-susceptible.ConclusionsResults show that free-ranging cattle and sheep are spreaders of Campylobacter as well as their AMR strains in the alpine environment. Therefore, contaminated alpine pastures or streams may constitute a source for the dissemination of AMR enteropathogens. However, apparently, alpine wild ungulates such as Pyrenean chamois play a negligible role in the epidemiology of zoonotic enteropathogens and AMR, and are not potential bioindicators of the burden of alpine environments.

Experimental results of functional characteristics of IOT for free range sheep breeding

Experimental results of functional characteristics of IOT for free range sheep breeding

Molecular Detection of Brucella melitensis Associated Reproductive Failure in Native Sheep Flock: Potential Public Health Pathogen from Tropical Region of Tamil Nadu

Brucellosis remains a global burden since the last century. In India, both animal and human brucella infections drastically increase every year. Outbreaks of 40 ewe’s abortion were investigated in native sheep’s from the tropical region of Tamil Nadu. Aborted maternal and fetal tissues were initially subjected to Stamps Modified Ziehl Neelsen (S-ZN) staining method. The DNA was extracted and screened for Brucella genome by Brucella cell surface salt extractable proteins (bcsp31) gene based PCR assay. Further, PCR positive samples were subjected for AMOS PCR for B. abortus, B. ovis, B. melitensis and B. suis identification and differentiation. S-ZN staining of aborted tissues revealed pink, coccobacilli organisms giving presumptive identification of Brucella sp. Molecular detection of brucellosis by bcsp31 gene based PCR in placental tissues and fetal abomasal contents of both the animals yielded a specific amplicon of 223 bp confirming it as Brucella genus infection. Molecular detection and differentiation by AMOS PCR revealed a specific amplicon of 731 bp indicating the involvement of B. meletensis infection in the native free-ranging sheep flock. It is a potent public health pathogen, highly essential to be monitored for the strategic control of brucellosis.

Consistent behavioural responses to heatwaves provide body condition benefits in rangeland sheep

Species are increasingly confronted with extreme climatic conditions such as heatwaves. Behavioural changes are an important response to changed environmental conditions. We investigated how free-ranging sheep (Ovis aries) in large rangeland paddocks respond to heatwave conditions in the arid zone of Australia. We defined heatwaves as a three-day period with each day’s maximum temperature exceeding 40 °C, and its minimum temperature remaining above 25 °C. Hence, the daily maximal temperature exceeded the upper limit of the thermal neutral zone in sheep. We equipped 48 sheep with GPS collars and combined the detailed locational data with georeferenced maps of water locations and tree areas, representing shade. We measured shade use as time spent within areas with trees. We show that individuals were consistent in their shade use behaviour between two separate heatwaves, while there was variation among individuals. Furthermore, shade use was positively related with body condition change between the start and the end of the study. In sheep body condition affects reproductive success, which suggests that shade use behaviour may have flow-on effects on reproductive benefits. We also compared shade use, water use and movement activity between the two heatwaves and two periods of typical ambient conditions. During heatwaves sheep spent on average 14.7 times longer in the shade during the midday interval and 2.0 times longer before dusk than during typical periods. Time spent near the water was high during the midday interval but did not differ between conditions, although, it was greater after dawn and before dusk during heatwaves. Such behavioural changes would assist in avoiding hyperthermia and dehydration. Sheep moved half the distance (which would include grazing) during the midday interval, which they seemed to offset to some extent through increased movement activity before and after dawn and dusk. Our study indicates that shade, and water use behaviours during hot ambient conditions upscale from small yards to large paddocks. Furthermore, it provides deep insight into the behavioural strategies that will allow a free-ranging arid zone ungulate to cope with the challenging ambient conditions that will become more prevalent with a changing climate.

Emergence of a Novel Ehrlichia minasensis Strain, Harboring the Major Immunogenic Glycoprotein trp36 with Unique Tandem Repeat and C-Terminal Region Sequences, in Haemaphysalis hystricis Ticks Removed from Free-Ranging Sheep in Hainan Province, China.

Ehrlichia minasensis, a recently described Ehrlichia species that is the most closely related to, but clearly distinct from, Ehrlichia canis, has been circulating in not only bovines, cervids, and dogs but also several tick species from Canada, Brazil, France, Pakistan, Ethiopia, and Israel. However, there are no reports of E. minasensis in China. The purpose of this study was to explore whether E. minasensis is present naturally in ticks in China. Through PCR targeting of the genus-conserved dsb gene, E. minasensis DNA was detected in Haemaphysalis hystricis ticks removed from free-ranging sheep in Hainan Province, South China in 2017. The partial sequence of the dsb, 16S rRNA, and groEL genes demonstrated that the Hainan strain shared 99% identity with the dsb gene of E. minasensis strain UFMG-EV (GenBank: JX629808), with the 16S rRNA of E. minasensis isolate E-2650 (MH500005) and with the groEL gene of E. minasensis strain UFMG-EV (JX629806), respectively. Moreover, sequence analysis of the major immunogenic tandem repeat protein (trp36) revealed that the Hainan strain harbored a unique tandem repeat sequence (APEAAPVSAPEAAPVSAPVS) and a C-terminal region that differed from those of other known E. minasensis strains. Additionally, phylogenetic analysis based on the entire amino acid sequence of trp36 revealed that the Hainan strain was closely related to a recently described E. minasensis strain from Brazil, of which the sister clade contained different strains of E. canis. The discovery of this novel Hainan strain in H. hystricis ticks represents the first known natural presence of E. minasensis in South China, highlighting the need for its constant surveillance.

Comparison of methods to estimate crude protein and digestible organic matter content of diets ingested by free-ranging sheep

Nutritional assessment of grazing ruminants, particularly on natural heterogeneous rangelands, remains a challenge for researchers and technicians. The aims of this study were: i) to compare methodologies of herbage sampling to determine crude protein (CP) and digestible organic matter (DOM) contents of ingested herbage and, ii) to evaluate the use of faecal near-infrared reflectance spectroscopy (fNIRS) to estimate those herbage attributes using either sampling procedure. Herbage samples were collected through hand-clipping (HC) or directly from rumen extrusa at the beginning (ExBM) and at the end (ExEM) of the longest daily sheep meal. In addition, DOM content of ingested herbage was also calculated from the amount of N excreted in faeces (FN). The study was conducted throughout thirteen consecutive months with six rumen-cannulated male sheep kept free on a rangeland plot in southern Brazil. Monthly, data on diet CP and DOM contents were collected by either method and related to near-infrared spectra of faeces collected from each respective sheep using the partial least squares method. Crude protein content in herbage ingested by sheep ranged from 108 to 172 g/kg DM and was, on average, lower (P < 0.05) in ExEM than in either ExBM or HC samples. Digestible organic matter content of ingested herbage, estimated through rumen in situ incubations, ranged from 546 to 717 g/kg DM and was, on average, similar for ExBM, ExEM, and HC samples. However, when this variable was estimated through the FN approach, values ranged from 446 to 601 g/kg DM and were, on average, lower (P < 0.05) than those found through other methods. The fNIRS models based on data obtained through extrusa (EX) and HC procedures produced similar and reliable results for estimating CP of herbage ingested by sheep, whereas the FN approach yielded the best fNIRS model for estimating DOM content of herbage.

Faecal N excretion as an approach for estimating organic matter intake by free-ranging sheep and cattle - CORRIGENDUM

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Faecal N excretion as an approach for estimating organic matter intake by free-ranging sheep and cattle

AbstractThe current study aimed to test whether organic matter intake by free-ranging ruminants could be estimated from the amount of nitrogen (N) excreted in faeces and to compare this approach to conventional techniques. An equation describing the relationship between excreted N and nutrient intake was developed in indoor digestibility trials conducted with male sheep (n= 36) and cattle (n= 24) housed in metabolism cages and solely fed hay harvested from a local rangeland. Faecal N excretion was linearly related to organic matter (OM) intake without a significant animal species effect. To evaluate the linear equation, data from free-ranging trials conducted with sheep and cattle were used. The faecal N approach was compared with eitherin situdigestibility plus external marker (n= 123) orn-alkanes (n= 272) to estimate OM intake and digestible OM intake. Estimates obtained through the faecal N approach did not closely fit those obtained with either conventional technique for any variable. Averaging all individual values, the supply of metabolizable energy (ME) estimated through faecal N was similar to the required level, whereas both thein situandn-alkanes techniques overestimated ME supply. In conclusion, OM intake by free-ranging sheep and cattle can be estimated based on the amount of N excreted in faeces with some advantages over conventional techniques: knowledge about herbage attributes is not required and it accounts for individual variability on selectivity and digestion processes.

Confirmation of free-ranging Barbary sheep Ammotragus lervia in Dghoumes National Park and Boukornine National Park, Tunisia

Confirmation of free-ranging Barbary sheep <i>Ammotragus lervia</i> in Dghoumes National Park and Boukornine National Park, Tunisia

Total protein and macroelement content in selected psammophilous grassland species under free-range sheep grazing in Kózki Nature Reserve

Total protein and macroelement content in selected psammophilous grassland species under free-range sheep grazing in Kózki Nature Reserve

Seed dispersal capacity of sheep and goats in a near-coastal dry grassland habitat

Our aim was to investigate the influence of seed dispersal via sheep and goats on the biodiversity of the characteristic plant community and endangered plant species in near-coastal dry grasslands. We examined epi- and endozoochory by sheep and goats, grazing with a shepherd in a protected dry grassland area in northern Germany. We recorded the species and density of seeds trapped in fur and excreted in faeces of sheep and goats. These two ungulates dispersed seeds of 44% of all the species from the study site. These included seeds from 31 locally endangered plant species. The diversity and number of seeds were higher in fur and faeces of sheep compared to goats. This pattern was also found for the occurrence of seeds from plants characteristic to this habitat and Red List species. Extrapolation of these observations to the whole flock of sheep (n=630) and goats (n=20) suggests that there are about 357,000 seed transported in the fur whereas up to 1,500,000 seeds are dispersed via endozoochory. These observations underline the importance of free-ranging sheep and goats in shaping the biodiversity of plant species in dry grasslands.