Progressive collapse resistance is more critical for precast concrete than cast-in-place structures. Most existing studies utilized scaled-down and quasi-static pushdown tests on substructures with rigid constraints under key member failure scenarios. However, the connection details, the spatial action of floors and the constraint stiffness of the remaining structure significantly affect the structure's progressive collapse resistance. Full-scale structural testing is the most direct method to evaluate the progressive collapse resistance of a new prefabricated structure, although it is difficult and costly to load and measure. A 2 × 2 bay 5-story full-scale frame structure was constructed according to the minimum requirements of the Code for Seismic Design of Buildings to investigate the progressive collapse resistance of a new precast, prestressed, efficiently fabricated frame (PPEFF) system. The structure's dynamic performance was analyzed by removing the 4th-floor edge column and bottom corner column. The slider and double micro-friction surface configuration proved suitable for the rapid removal of large axial force columns in a full-scale progressive collapse experiment. The layout of the full-scale structural test and the structure's dynamic responses are described. The effect of the lateral constraint stiffness and the duration of column removal on the dynamic response of the test structure were analyzed using finite element analysis. The progressive collapse resistance mechanism of the PPEFF structure was discussed. The PPEFF system remained in an elastic state and exhibited good progressive collapse resistance.