Frame Lake Research Articles

Intra-lake response of Arcellinida (testate lobose amoebae) to gold mining-derived arsenic contamination in northern Canada: Implications for environmental monitoring

Arcellinida (testate lobose amoebae) were examined from 40 near-surface sediment samples (top 0.5 cm) from two lakes impacted by arsenic (As) contamination associated with legacy gold mining in subarctic Canada. The objectives of the study are two folds: quantify the response of Arcellinida to intra-lake variability of As and other physicochemical controls, and evaluate whether the impact of As contamination derived from two former gold mines, Giant Mine (1938–2004) and Tundra Mine (1964–1968 and 1983–1986), on the Arcellinida distribution in both lakes is comparable or different. Cluster analysis and nonmetric multidimensional scaling (NMDS) were used to identify Arcellinida assemblages in both lakes, and redundancy analysis (RDA) was used to quantify the relationship between the assemblages, As, and other geochemical and sedimentological parameters. Cluster analysis and NMDS revealed four distinct arcellinidan assemblages in Frame Lake (assemblages 1–4) and two in Hambone Lake (assemblages 5 and 6): (1) Extreme As Contamination (EAC) Assemblage; (2) High calcium (HC) Assemblage; (3) Moderate As Contamination (MAC) assemblages; (4) High Nutrients (HN) Assemblage; (5) High Diversity (HD) Assemblage; and (6) Centropyxis aculeata (CA) Assemblage. RDA analysis showed that the faunal structure of the Frame Lake assemblages was controlled by five variables that explained 43.2% of the total faunal variance, with As (15.8%), Olsen phosphorous (Olsen-P; 10.5%), and Ca (9.5%) being the most statistically significant (p < 0.004). Stress-tolerant arcellinidan taxa were associated with elevated As concentrations (e.g., EAC and MAC; As concentrations range = 145.1–1336.6 mg kg−1; n = 11 samples), while stress-sensitive taxa thrived in relatively healthier assemblages found in substrates with lower As concentrations and higher concentrations of nutrients, such as Olsen-P and Ca (e.g., HC and HM; As concentrations range = 151.1–492.3 mg kg−1; n = 14 samples). In contrast, the impact of As on the arcellinidan distribution was not statistically significant in Hambone Lake (7.6%; p-value = 0.152), where the proportion of silt (24.4%; p-value = 0.005) and loss-on-ignition-determined minerogenic content (18.5%; p-value = 0.021) explained a higher proportion of the total faunal variance (58.4%). However, a notable decrease in arcellinidan species richness and abundance and increase in the proportions of stress-tolerant fauna near Hambone Lake’s outlet (e.g., CA samples) is consistent with a spatial gradient of higher sedimentary As concentration near the outlet, and suggests a lasting, albeit weak, As influence on Arcellinida distribution in the lake. We interpret differences in the influence of sedimentary As concentration on Arcellinida to differences in the predominant As mineralogy in each lake, which is in turn influenced by differences in ore-processing at the former Giant (roasting) and Tundra mines (free-milling).

Sub-bottom acoustic profiling as a remediation assessment tool for contaminated lakes

Frame Lake, a small (88.4 ha), shallow (< 6.5 m maximum depth), high-latitude lake found within the city limits of Yellowknife, Northwest Territories, Canada was selected due to the known legacy contamination of the lake’s sediments to test the feasibility of using seismic sub-bottom profiling to estimate total volumes of heavy metal contaminated sediments in lacustrine environments. To ground-truth the sub-bottom profiling results, physical and ICP-MS analyses were carried out on freeze cores collected from Frame Lake’s southern basin, and sedimentological marker beds and 14C dating was used to chronologically constrain the lake depositional history. ICP-MS results showed high levels of arsenic contamination (up to 1538 µg g−1) in late twentieth-century lake sediments, which contrasts sharply with measured Holocene values that averaged only 16 µg g−1 (n = 41, ± 5.4 SD). The high arsenic content in lakebed sediments, which tends to be concentrated within specific horizons, results in distinct seismic reflectors within the acquired Sonar data. Stratigraphic horizons where arsenic was concentrated do not necessarily correlate with actual depositional events as changes in lake hydrology and redox conditions have resulted in remobilization and migration of arsenic in lake sediments. Direct GIS software comparison of core data against the sub-bottom profiler transect results permitted an interpolated lateral and vertical reconstruction of the distribution of variously contaminated sediments throughout the entire lake basin. Based on our analysis, a minimum of ~ 230,000 m3 of contaminated sediments would need to be dredged from Frame Lake to achieve a minimum residual sediment arsenic concentration of < 150 µg g−1.

What killed Frame Lake? A precautionary tale for urban planners.

Frame Lake, located within the city of Yellowknife, Northwest Territories, Canada, has been identified as requiring significant remediation due to its steadily declining water quality and inability to support fish by the 1970s. Former gold mining operations and urbanization around the lake have been suspected as probable causes for the decline in water quality. While these land-use activities are well documented, little information is available regarding their impact on the lake itself. For this reason, Arcellinida, a group of shelled protozoans known to be reliable bioindicators of land-use change, were used to develop a hydroecological history of the lake. The purpose of this study was to use Arcellinida to: (1) document the contamination history of the lake, particularly related to arsenic (As) associated with aerial deposition from mine roaster stacks; (2) track the progress of water quality deterioration in Frame Lake related to mining, urbanization and other activities; and (3) identify any evidence of natural remediation within the lake. Arcellinida assemblages were assessed at 1-cm intervals through the upper 30 cm of a freeze core obtained from Frame Lake. The assemblages were statistically compared to geochemical and loss-on-ignition results from the core to document the contamination and degradation of conditions in the lake. The chronology of limnological changes recorded in the lake sediments were derived from 210Pb, 14C dating and known stratigraphic events. The progress of urbanization near the lake was tracked using aerial photography. Using Spearman correlations, the five most significant environmental variables impacting Arcellinida distribution were identified as minerogenics, organics, As, iron and mercury (p < 0.05; n = 30). Based on CONISS and ANOSIM analysis, three Arcellinida assemblages are identified. These include the Baseline Limnological Conditions Assemblage (BLCA), ranging from 17–30 cm and deposited in the early Holocene >7,000 years before present; the As Contamination Assemblage (ACA), ranging from 7–16 cm, deposited after ∼1962 when sedimentation began in the lake again following a long hiatus that spanned to the early Holocene; and the Eutrophication Assemblage (EA), ranging from 1–6 cm, comprised of sediments deposited after 1990 following the cessation of As and other metal contaminations. The EA developed in response to nutrient-rich waters entering the lake derived from the urbanization of the lake catchment and a reduction in lake circulation associated with the development at the lake outlet of a major road, later replaced by a causeway with rarely open sluiceways. The eutrophic condition currently charactering the lake—as evidenced by a population explosion of eutrophication indicator taxa Cucurbitella tricuspis—likely led to a massive increase in macrophyte growth and winter fish-kills. This ecological shift ultimately led to a system dominated by Hirudinea (leeches) and cessation of the lake as a recreational area.

Biogeochemical evidence of eutrophication and metal contamination of Frame Lake, City of Yellowknife, Northwest Territories, Canada

The sediments of Frame Lake, Yellowknife, NWT were examined to evaluate human impact due to urbanization and mining and provide background information for potential remediation efforts. Physical and geochemical analyses were carried out on cores from two sites targeting the lake’s deep basins. Pb-210 analysis was used to establish chronological control and determine recent sedimentation rates. Frame Lake contains sediments spanning the whole of the Holocene from the time of deglaciation to the present. With the development of the city and increasing human population over the last ~80 years Frame Lake has become increasingly eutrophic. The source of nutrient loading is generalized and probably not exceptionally high; however, due to its limited depth, extensive winter ice cover and low turnover rates, the lake appears to be sensitive to the resulting biological oxygen demand. Frame Lake sediments were found to contain elevated concentrations of metals attributable to gasoline combustion (Pb), mining and ore processing (e.g. As, Cu) and other sources (Zn). While most of these elements peak below the sediment surface and contamination has decreased in recent years, concentrations remain above levels recommended for the protection of aquatic life. It is apparent that contaminants previously deposited within the catchment are still supplying the lake and in the case of As, that post-depositional translocation within the sediments is taking place.