Fragmented Areas Research Articles

Esports Training, Periodization, and Software—A Scoping Review

Electronic sports (esports) and research on this emerging field are interdisciplinary in nature. By extension, it is essential to understand how to standardize and structure training with the help of existing tools, developed over years of research in sports sciences and informatics. Our goal for this work is to review the available literature in esports research, focusing on sports sciences (training, periodization, planning, and career stages) and software (training tools, visualization, analytics, and feedback systems). To verify the existing sources, we applied the framework of a scoping review to address the search from multiple scientific databases with further local processing. We conclude that the current research on esports has mainly focused on describing and modeling performance metrics that span over multiple fragmented research areas (psychology, nutrition, informatics). However, these building blocks have not been assembled into a well-functioning theory of performance in esports by, e.g., providing exercise regimes or methods of periodization for esports.

Body size and abundance patterns of male orchid bees in a fragmented Neotropical landscape

Abstract Understanding the relationship between functional traits and fragmentation is crucial for promoting biodiversity and ecosystem function. Orchid bees (Euglossini) are important pollinators in Neotropical forests, but how body size may be associated with fragmentation and its influence on community organisation remains unexplored. We systematically sampled orchid bee communities across 15 plots in a fragmented area in the Ecuadorian Amazon basin to evaluate how patch size and isolation are associated with body size and abundance. Our samples comprised large‐bodied bees (Eulaema, Euglossa and the parasitic Exaerete), with small Euglossa notably absent. The relative representation of Eulaema species of varying sizes changed across the fragmented landscape, leading to shifts in overall mean body size consistent with the prediction that larger‐bodied pollinators are better at long‐distance movement. Euglossa, the most abundant genus (67% of the total sample), was found across all patches regardless of body size. Euglossa intersecta and Euglossa orellana, the largest Euglossa in the region, were the most common species across the fragmented landscape. The abundance of male euglossines experienced a general decline with decreasing patch size and increasing isolation. However, at the species level, the abundance of some species was not affected at all. Because body size often mediates dispersal ability, thermoregulation, and resource use, we reinforce the importance of assessing body size patterns to understand changes in diversity, abundance and the composition of tropical pollinator communities in fragmented landscapes.

Differential predation pressure on island pit vipers shape their niches and defensive behavior

Abstract Animals are naturally exposed to several predators, and, given that a single encounter with a predator may cause prey’s death, natural selection will strongly act upon its defensiveness. Therefore, defensive behavior is strongly shaped by predation pressure. Predation rate in islands tends to be relaxed due to lower abundance of predators. The aim of this study was to test the hypothesis that predation rates upon the two island pit vipers Bothrops alcatraz and B. insularis would be lower than correlated mainland B. jararaca. Additionally, we evaluated the defensive behavior of these island snakes in the field to compare with the mainland B. jararaca. Predation pressure upon the insular species was assessed utilizing plasticine replicas, and we evaluated their defensive behavior to approach. These data were compared to those previously reported for B. jararaca. We found a lower predation pressure on B. insularis (from the smaller area) than on B. alcatraz and B. jararaca from the urban mainland forest fragment (areas with intermediate size), and all of them showed lower predation pressure than B. jararaca from the continuous mainland forested area. In the field, island snakes show narrower defensive repertoire than B. jararaca, since most B. insularis fled, and B. alcatraz either fled or remained immobile on approach. Apparently, the predation pressure to which these island pit vipers are exposed shapes their defensive behavior, as well as the niches exploited by them.

Regional variation in the landscape ecology of West Nile virus sentinel chicken seroconversion in Florida.

How landscape composition and configuration impact the distribution of multi-vector and multi-host mosquito vector-borne disease systems, such as West Nile virus (WNV), remains challenging because of complex habitat and resource requirements by hosts and vectors that affect transmission opportunities. We examined correlations between landscape composition and configuration and 2018 WNV sentinel chicken seroconversion in Florida, USA across the state and within five National Oceanic Atmospheric Administration (NOAA) bioclimatic regions to understand strength and variation of landscape effects during an elevated transmission year. Although few landscape studies have examined WNV in Florida, we expected higher percentages of residential or medium-developed landscapes and more fragmented landscapes would be positively correlated with WNV seroconversion owing to the main mosquito vector habitats and avian host distributions. However, we expected to find variation in the importance of forest, wetland, and agriculture landscapes across bioclimatic regions in the state. WNV seroconversion rates were calculated using Florida 2018 Department of Health WNV sentinel chicken seroconversion data from 187 flocks maintained by mosquito control programs. Percent land cover and edge density metrics were calculated for multiple land cover classes and within multiple buffer distances from chicken coops using 2019 National Land Cover Data. We used binomial generalized linear mixed effects models to calculate the importance of landscape metrics to WNV seroconversion. We found no statewide predictors of seroconversion, but as expected, the importance of landscape varied across regions. In the north-central part of the state, we found higher seroconversion in less populated suburban areas while higher seroconversion in south-central Florida was correlated with fragmented forested areas within 0.5 km of coops and intact woody wetland areas within 2 km of coops. This work corroborates previous findings that consistent landscape predictors of WNV are difficult to identify across broader geographic areas and sets the stage for additional work that incorporates climate and landscapes interactions for a greater understanding of WNV ecology in this geographic region.

Forest fragmentation effects on plant-animal interaction do not always show consistent patterns – Evidence from a seed removal experiment of 31 woody species

Forest fragmentation often leads to changes in the abundance and composition of various organisms, thereby affecting biotic interactions. Currently, many studies lack consensus on the magnitude or direction of the impact of forest fragmentation on plant-animal interactions. Here, we employed seed-rodent interaction as a case study and assessed the removal by rodents of 66,960 seeds belonging to 31 woody species in 18 fragmented forests with areas of 1.05–14,517.63 ha, in a seriously fragmented tropical area in Southwest China for two consecutive years. Our study provided strong evidence that forest fragmentation affected seed-rodent interactions but did not follow a consistent pattern between years. Forest size and edge effects on seed removal were mainly due to variations in seed availability but not rodent activity. Interestingly, the effect of fragment size on seed removal was species-specific with monotonic decreases as well as U-shaped and humped-shaped patterns. These remarkable spatiotemporal and interspecific variations pertaining to forest fragmentation impact on seed removal may translate into subsequent seed germination and seedling regeneration processes, which may further influence the species composition in different fragmented forests. This complexity in responses among plant species reiterates the need to consider the interspecies specificity in conservation and management initiatives of fragmented forests. Additionally, we recommend that multiple field surveys involving multiple species be conducted in future studies focusing on forest fragmentation to help us better understand fragmentation effects on ecological processes.

Immediate impacts of fire on koala movement in a fragmented landscape

Hazard reduction burns could pose a significant conservation challenge to threatened habitat specialists, such as koalas Phascolarctos cinereus. This study examines the immediate effects of a medium to hot hazard reduction burn on a small number of koalas occupying a fragmented agricultural area. Three koalas being monitored using GPS telemetry were inadvertently exposed to fire in a small strip of roadside vegetation in an agricultural landscape, providing an unexpected opportunity to assess their immediate responses to the fire. Nearly 81% of available trees were burnt to some degree, with 31% of tree foliage scorched up to 10.2m above the ground. The koalas reduced their home range sizes by 20–54% post-fire, where two avoided burnt areas while one remained within them. These findings highlight the importance of maintaining unburnt patches and corridors in fragmented landscapes and developing comprehensive conservation plans to mitigate the adverse effects of fire on koalas and other arboreal fauna.

Evaluating urban biodiversity: Effectiveness of citizen science driven species distribution models in urban ecosystems

Citizen science (CS) and remote sensing (RS) approaches have become more reliable, thus providing higher resolution and generating a large amount of environmental data. When considering urban environments, where fragmented and highly diverse landscapes are predominant, the combination of citizen science data and remote sensing techniques with species distribution models (SDM) can play a vital role in comprehensively investigating and evaluating urban biodiversity. However, citizen science derived species distribution models for multiple avian species in dense and fragmented urban areas are rarely used. The study aims (I) to elaborate, whether CS driven SDMs can be effectively used in spatially complex urban environments; (II) to identify biodiversity hotspots and prioritize areas for nature conservation and (III) to examine, if existing protective areas correspond to species’ hotspots. Therefore, Citizen science-based datasets of 26 breeding bird species over three years were obtained for this analysis in Germany’s Ruhr Metropolis. Quality assurance, data thinning, and pseudo-absence simulations were performed. Spatial data from the ecosystem LiDAR project GEDI (Global Ecosystem Dynamics Investigation), climate data from the German Weather Service, and land use information from Copernicus were used as environmental predictors. Eleven different species distri-bution models (SDMs) were trained on species subselection by using Biomod2 for preliminary analysis. Overall model performance was evaluated via several metrics, including TSS (true skill statistics) and ROC (receiver operating characteristic). Finally, four species distribution models were used for ensemble modelling. Subsequently, a species richness analysis was performed with the aim of identifying spots with high avian biodiversity. Overall, the CS-derived SDMs performed well, with high predictive power for all of the investigated species. Within the Ruhr Metropolis, approximately 6% (250 km²) of the terrain was identified as being highly suitable for avian diversity, inhabiting at least 17 out of 26 species. Predominantly within the core urban areas, high species richness was predicted on preserved brownfields and revitalized mine sites, as well as in the remnants of formerly demarcated regional greenbelts. Additionally, regions outside of the core area, which are part of the overarching biotope network framework, proved to have high species richness capabilities for avian biodiversity. These findings aid in optimizing urban development concepts and (sub)urban green space management with respect to urban biodiversity conservation. Following the implications of the recently established Regional Biodiversity Strategy in the Ruhr Metropolis, this analysis demonstrates the importance of networked green spaces, their preservation and the need to close existing network gaps within the Ruhr Metropolis.

Impact of microstructure of whey protein gels on in vitro gastric protein digestion is sustained after oral structural breakdown by mastication

Gastric protein digestion can be influenced by food micro- and macrostructure. Understanding the interplay between microstructure and macrostructural breakdown during oral processing is crucial to enhance the nutritional value of protein-rich foods. This study compared the in vitro gastric protein digestion of whey protein gels differing in microstructure before and after mastication. Whey protein isolate was mixed with κ-carrageenan to obtain heat-induced gels differing in microstructure (homogeneous, coarse stranded, protein continuous, bi-continuous). Gel boli were collected from 14 participants. The number, size and total surface area of bolus fragments were determined. In vitro gastric protein digestion was quantified following the INFOGEST 2.0 protocol with minor modifications. Before mastication, coarse stranded gels showed the highest digestion rate (1.07 mmol L−1∙g dry matter−1/h), while homogeneous, protein continuous and bi-continuous gels showed lower and similar digestion rates (0.82–0.87 mmol L−1∙g dry matter−1/h). After mastication, the total surface area of coarse stranded gels increased 7.9-fold leading to a 1.9-fold increase in digestion rate. In contrast, a 3.4-fold increase in total surface area of bi-continuous gels caused a 2.8-fold increase in digestion rate. The total surface area of homogeneous and protein continuous gels increased 3.1- to 3.6-fold upon mastication resulting in a 1.7- to 1.9-fold increase in digestion rate. The increase in protein hydrolysis did not correlate with the degree of structural breakdown after mastication across gels differing in microstructure. We conclude that the impact of microstructure of whey protein gels on in vitro gastric protein digestion is sustained after oral structural breakdown by mastication.

Assessment of coastal landscape fragmentation and its driving factors based on optimal scale: A case study of the Yellow River Delta, China

Since the large-scale oil exploration and development in the 1960 s, the Yellow River Delta (YRD) has undergone rapid industrialization and urbanization. Concurrently, the regional landscape ecological structure has experienced significant changes, and the degree of landscape fragmentation has increased. Therefore, a comprehensive assessment of the spatiotemporal dynamics and formation mechanisms of landscape fragmentation is crucial for the protection and restoration of natural ecosystem in the YRD. This study constructs an evaluation index system for landscape fragmentation in the YRD from three perspectives: quantitative characteristics, shape features, and spatial distribution. By combining inflection point analysis, landscape area loss index, and geostatistical models, the optimal spatial scale of landscape fragmentation from 2000 to 2020 is determined. Additionally, the study analyzes the spatiotemporal changes in landscape fragmentation and employs the optimal parameters-based geographical detector (OPGD) model to reveal the formation mechanisms of spatial heterogeneity in landscape fragmentation. The study findings are as follows: (1) The optimal spatial resolution for analyzing landscape fragmentation in the YRD was 80 m, and the extent was 880 m. (2) Between 2000 and 2020, landscape fragmentation in the YRD increased, with the highest level recorded in 2020. Highly fragmented areas, particularly inland regions, expanded remarkably, whereas coastal areas experienced lower fragmentation levels. (3) Distance to rivers and ditch density were the primary factors affecting landscape fragmentation, followed by land-use type and average land gross domestic product. The influence of natural factors was relatively minimal. The interaction between factors had a greater influence on landscape fragmentation compared to individual factors. The distance to rivers and land-use type exhibited the most substantial enhancement effects following interaction with other factors. This study provides valuable insights into the spatiotemporal dynamics and mechanisms of landscape fragmentation in the YRD, providing valuable guidance for the sustainable management and ecological restoration of the region and other similar ecologically sensitive areas.

Integrating high-resolution remote sensing and empirical wildlife detection data for climate-resilient corridors across tropical elevational gradients

Corridors are essential tools for promoting biodiversity resilience under climate change. However, corridor design studies are often conducted at spatial scales too coarse to guide implementation by local conservation practitioners. We mapped potential climate-resilient corridors linking lowland to highland protected areas within a highly biodiverse but fragmented landscape of southwestern Costa Rica (6311 km2) using least cost path and circuit theory approaches at high spatial resolution (10 m). We then applied an extensive camera trap dataset of medium-large vertebrates to examine corridor functionality. Although least cost paths (n = 40) were predominantly forested (median = 76 %, range = 57–82 %) and somewhat protected (median = 31 %, range = 3–55 %), they were also highly fragmented. Least cost paths from lowland to highland protected areas traversed medians of 252 forest patches (range = 162–328), 11,186 agriculture patches (range = 822–1,771), and 106 roads (range = 50–252), translating to 2 forest patches, 11 agriculture patches, and 1 road crossed every kilometer. Circuit analyses identified many high-connectivity areas outside of protected areas, including but not limited to least cost paths, but these high-connectivity areas were mostly small forest fragments. Nonetheless, capture rates for medium-to-large mammals at camera traps indicated that many species are currently unlikely to use unprotected, fragmented areas thought to be important for connectivity. In other words, additional conservation and restoration are necessary to establish functional corridors within the landscape. More broadly, this study exemplifies an approach to bridging the gap between regional-scale connectivity analyses and the needs of local practitioners by identifying locations that could be targeted for conservation or restoration within multi-use tropical landscapes.

Forest fragmentation effects on mutualistic interactions: frugivorous birds and fruiting trees

While many effects of forest fragmentation are reasonably well understood, knowledge of interspecific interactions in fragmented ecosystems is much more limited, particularly for high diversity tropical forests. Using nearly 40 years of data from the Biological Dynamics of Forest Fragments Project in Central Amazonia, we assessed whether forest fragment area and time since isolation impact mutualistic interactions between frugivorous birds and their food resources. We used structural equation modeling to analyze the complex pathways between four main variables determining these interactions: fruiting tree abundance, frugivorous bird abundance, forest fragment area, and time since fragment isolation. Our results confirm that fragment area alters the abundance of some tree resources, with successional plant families increasing in abundance with decreasing fragment size. However, these changes do not drive alterations in the abundance of frugivorous birds. We also tested if bird species with a greater relative diet breadth are less vulnerable to forest fragmentation and found that specialist frugivores are more vulnerable to forest fragmentation immediately after isolation but are not differentially impacted within the long term. Collectively, our results demonstrate the need to further evaluate human‐driven habitat change across multiple timescales to fully understand its impacts on complex species interactions.

Inhibited hydrogen uptake in metasomatised cratonic eclogite

Water occurs in Earth’s interior mostly as trace hydroxyl in nominally anhydrous minerals. Clinopyroxene is known to be an important water carrier in the uppermost mantle, and eclogite, which forms a subordinate part of the cratonic lithosphere, contains some 50% of jadeite-rich clinopyroxene, making this potentially a significant H2O reservoir in the bulk lithospheric mantle. Mantle metasomatism, in particular by small-volume melts like kimberlite, is known to enrich the lithosphere in highly incompatible components, but its effect on H2O contents in cratonic eclogite remains unclear. We report H2O concentrations for clinopyroxene and garnet in eclogite and pyroxenite xenoliths from several African kimberlites, obtained by Fourier-Transform Infrared Spectroscopy (FTIR). Except one sample showing evidence for minor within-grain variability of H2O concentrations (< 15%), FTIR images demonstrate that H2O is homogeneously distributed in optically clear areas of clinopyroxene fragments mounted for this study. The samples were variably metasomatised by a kimberlite-like melt, as evidenced by elevated MgO contents and abundances of highly incompatible elements (e.g., Sr, Ce, Th). Although metasomatised eclogites and pyroxenites on average show higher H2O abundances than pristine ones, mantle metasomatism decreases the Al2O3 content in clinopyroxene, which is known to enhance hydrogen incorporation in this mineral. As a consequence, hydrogen incorporation is inhibited, and c(H2O) becomes increasingly decoupled from other highly incompatible components, such as LREE. Thus, eclogite – metasomatised or not - does not significantly contribute to the H2O inventory in the bulk cratonic mantle.

Urban landscape affects scaling of transportation carbon emissions across geographic scales

Understanding the carbon dynamics of the transportation sector is necessary to mitigate global climate change. While urban scaling laws have been used to understand the impact of urban population size on carbon efficiency, the instability of these scaling relationships raises additional questions. Here, we examined the scaling of on-road transportation carbon emissions across 378 US metropolitan statistical areas (MSAs) using diverse urban landscape patterns and spatial units, from the MSA level down to 1 km grid cells. Beginning with a baseline scaling model that uses only population size, we expanded the model to include landscape metrics at each spatial scale based on correlation results. We found that: (1) urban landscape characteristics provide insights into carbon mechanisms not fully captured by population size alone, (2) the impact of population size on on-road carbon emissions transitions from linear to sub-linear scaling relationships as the geographic scale of analysis decreases, and (3) clustered urban developments can form carbon-efficient landscapes, while fragmented urban areas tend to be carbon-inefficient. Based on empirical evidence, this research advocates for hierarchical spatial planning and supports the implementation of policy measures aligned with smart growth principles to mitigate carbon pollution.

Tibetan Birchbark Manuscripts in the Serindian Collection of the IOM, RAS: Restoration and Identification

The article presents the Tibetan birchbark manuscripts kept in the Serindian collection of the IOM, RAS. The five birchbark manuscripts exhibit common codicological and paleographic characteristics, and the texts within them demonstrate a functional congruence, primarily associated with the rituals of consecration and empowerment. The objective of the conservation efforts was to flatten the birchbark fragments to both identify and preserve the inscribed texts. The fragments were sufficiently moistened in the birch sap as a plasticizer instead of regular water, given that it is a natural substance inherent to birch bark. The birch sap for conservation was hand-harvested from an ecologically pristine area. The soaking process in warm birch sap softened the fragments, and they were also cleansed of surface impurities. Damaged areas of the birchbark fragments were reinforced using Japanese conservation paper. For subsequent use and preservation, the fragments were stored in envelopes made of high-quality Melinex polyester film. Owing to the restoration work, five Tibetan birchbark manuscripts were described and transliterated.

Food Sovereignty in Nepal : A Current Appraisal

Food sovereignty issue is integrated with agro ecology, climate and environmental justice, right of food workers, agrarian reform and justice to women and peasants. Despite of the promises of food security by the use of new practices and technologies global hunger has significantly increased. Industrial food production and indiscriminant use of chemical fertilizers pesticides have caused air, water and soil pollution and consequently environment and human health has been seriously impaired. Nepal being a developing economy could hardly endow all her possible resources just to address food security issue, hence the food sovereignty issue remained veiled till 2075B.S. Present study aims to enquire the working status of food sovereignty principal on real grounds in the country. The study is based on secondary data and is quantitative in nature. As pre design it is descriptive and prescriptive both. The study examines efficacy of food sovereignty principal on six pillar of it which are: focus on food for people; Value food providers; localize food system; puts control locally; builds knowledge and skills and work with nature. Examining through facts and figures, it is found that there is food crisis in the country with its quantity and nutritive value. The extended net works of cooperative throughout the country may play significant role in activating food sovereignty principals. Existences of middle man, poorer financial assistance, poorer insurance coverage, limitation of minimum price support policy to fewer crops are some of the major problems. In addition land fragmentation, diminishing wet and dry land area are additional bottlenecks. Indiscriminant use of chemical fertilizers and pesticides pose a crucial problem to practice organic farming. Activities as river bed farming, tharu alu cultivation, Jhol mal preparation, kitchen gardening, community seed bank and use of local biochar are working well with positive outcomes to support food sovereignty efforts.

Purpose in Management Research: Navigating a Complex and Fragmented Area of Study

Purpose in Management Research: Navigating a Complex and Fragmented Area of Study

Impact of Fragmentation on Carbon Uptake in Subtropical Forest Landscapes in Zhejiang Province, China

Global changes cause widespread forest fragmentation, which, in turn, has given rise to many ecological problems; this is especially true if the forest carbon stock is profoundly impacted by fragmentation levels. However, the way in which forest carbon uptake changes with different fragmentation levels and the main pathway through which fragmentation affects forest carbon uptake are still unclear. Remote sensing data, vegetation photosynthesis models, and fragmentation models were employed to generate a time series GPP (gross primary productivity) dataset, as well as forest fragmentation levels for forest landscapes in Zhejiang province, China. We analyzed GPP variation with forest fragmentation levels and identified the relative importance of the phenology (carbon uptake period—CUP) and physiology (maximum daily GPP—GPPmax) control pathways of GPP under different fragmentation levels. The results showed that the normalized mean annual GPP data of highly fragmented forests during the period from 2000 to 2018 were significantly higher than those of other fragmentation levels, while there was almost no significant difference in the annual GPP trend of forest landscapes with all fragmentation levels. Moreover, the percentage area of the control variable, GPPmax, gradually increased with fragmentation levels; the mean GPPmax between 2000 and 2018 of high-level fragmentation was higher than that of other fragmentation levels. Our results demonstrate that the carbon uptake capacity per unit area was enhanced in highly fragmented forest areas, and the maximum photosynthetic capacity (physiology-based process) played an important role in controlling carbon uptake, especially in highly fragmented forest landscapes. Our study calls for a better and deeper understanding of the potential of forest carbon uptake, and it is necessary to explore the mechanism by which forest fragmentation changes the vegetation photosynthetic process.

Synergistic effects of glial cell line-derived neurotrophic factor and base-medium on in vitro culture of testicular tissue derived from prepubertal collared peccary.

We evaluated the influence of different media plus various concentrations of Glial cell line-derived neurotrophic factor (GDNF) during the in vitro culture (IVC) of testicular tissues from prepubertal collared peccary. Testes from 5 individuals were collected, fragmented and cultured for 28 days (34°C and 5% CO2). Culture media were Dulbecco's modified essential medium (DMEM) or stem cell serum free media (StemPro-34™ SFM), both supplemented with various concentrations of GDNF (0, 10, or 20 ng/mL). Fragments were cultured on the flat surface of 0.75% agarose gel and were evaluated every 7 days for fragment area, histomorphology, cellular viability, and proliferative activity. Data were expressed as mean ± standard error and analyzed by Kruskal-Wallis's and Tukey test. Fragments area decreased over the 28 days-culture, regardless of the treatment. For morphology, the StemPro-37 SFM medium plus 10 ng/mL GDNF provided higher scores at all time points in comparison to DMEM using any GDNF concentration (p < .05). After 28 days, similar cellular viability (~70%) was observed in all treatments (p > .05). For proliferating cell nuclear antigen assay, only DMEM plus 10 ng/mL GDNF improved (p < .05) cellular proliferation on Days 14 and 28. Looking at argyrophilic nucleolar organizing regions, after 28 days, there were no differences among treatments regarding cell proliferative capacity for both spermatogonia and Sertoli cells (p > .05). In summary, the DMEM and StemPro-34 SFM are adequate medium for IVC of prepubertal peccary testicular tissue. Supplementation with GDNF, especially at a 10 ng/mL concentration, appears to be essential for the maintenance of cell survival and proliferation.

Evaluation of Ecological Sensitivity and Spatial Correlation Analysis of Landscape Patterns in Sanjiangyuan National Park

The Sanjiangyuan region, situated on the Qinghai–Tibetan Plateau, constitutes an exceptionally delicate ecological environment. Alterations in the region’s ecological landscape stem not only from natural factors but also from significant anthropogenic influences, exerting a notable impact on the sustainable economic and social development of the region’s middle and lower reaches. Consequently, investigating changes in the landscape pattern of Sanjiangyuan National Park holds paramount importance for comprehending the formation mechanism of spatial landscape distribution in the area. This study analyzes the ecological sensitivity and landscape pattern of Sanjiangyuan National Park in Qinghai Province, China, utilizing ArcGIS 10.8 and Fragstats 4.2. Employing the bivariate spatial autocorrelation analysis method, the research uncovers the spatial distribution characteristics between ecological sensitivity and landscape pattern, along with their aggregated change traits. The findings reveal that ecological sensitivity areas within the park encompass varying degrees, ranging from extremely sensitive to insensitive. The area of moderately sensitive zones in the Yellow River source region is 7279.67 km2 (39.17%), whereas the corresponding area in the Yangtze River source region is 32,572.34 km2 (36.30%). The eastern and northern parts of the Sanjiangyuan National Park exhibit significant landscape fragmentation. Ecological sensitivity varies markedly across different regions, with the southern and some northern areas showing higher sensitivity. In the Lancang River source park and the southern part of the Yellow River source park, the Largest Patch Index (LPI) and Ecological Sensitivity Index exhibit a high–high (HH) clustering pattern, indicating strong ecological connectivity in these areas. These regions also feature high Total Edge (TE), Number of Patches (NP), Patch Density (PD), and Edge Density (ED), indicating a complex landscape structure and abundant habitat edge areas. The study recommends restoring ecological connectivity in highly fragmented areas and implementing strict protection measures in sensitive regions to maintain ecosystem health and biodiversity. These findings provide a foundation for developing targeted ecological protection measures to enhance ecosystem health and biodiversity conservation in the area. This research aligns with several Sustainable Development Goals (SDGs), including Climate Action, Life on Land, and Clean Water and Sanitation, by promoting sustainable ecosystem management and biodiversity conservation.

Effects of Platelet-Rich Plasma and Concentrated Growth Factor on Viability of Ultra-Diced Cartilage Grafts in a Rabbit Model

Although rhinoplastic surgery has progressed considerably in recent years, nasal dorsal irregularities still cause postoperative distress for both surgeons and patients. The aim of this study was to measure the association between two biologic graft adjuncts, platelet-rich plasma (PRP) and concentrated growth factor (CGF), and ultra-diced cartilage viability in an animal model. This study was designed as a randomized in-vivo study using a rabbit model. Fourteen rabbits were utilized in this investigation. The ultra-diced cartilage was obtained from auricular cartilage. The graft biologic adjunct is the predictor variable. There were three treatment groups: graft mixed with PRP or CGF or untreated (control). The grafts were placed in three separate pockets opened on the same rabbit. Grafts were harvested 3months after insertion for analysis. The primary outcome variable was histopathological and regenerative scores obtained from multiple histopathological parameters indicating the viability of the cartilage. Histopathological score parameters were chondrocyte loss, inflammation, fibrosis, cartilage fragmentation, and calcified area formations in the lacunae. Regenerative score parameters were peripheral cell proliferation in the cartilage tissue, vascularization in the connective tissue, proteoglycan increase in the matrix, and the amount of connective tissue. The variables were age, sex, and weight. Statistical analysis employed the analysis of variance test, with a significance level of P<.05. The sample was composed of 14 rabbits and 42 samples. The histopathologic scores were 11.93 (±2.49), 8.78 (±2.19), and 6.85 (±1.46) for the control, PRP, and CGF groups, respectively. A statistically significant difference was found in the PRP (P<.0275) and CGF (P<.0001) groups compared to the control group. The regenerative scores were 6.21 (±0.97), 8.85 (±1.70), and 12.07 (±1.26) for the control, PRP and CGF groups, respectively. A statistically significant difference was found in the PRP (P<.0159) and CGF (P<.0001) groups compared to the control group. This is the first study investigating the ultra-diced cartilage graft in an experimental animal model. Histopathological examination has shown that mixing ultra-diced cartilage with CGF or PRP increases viability by reducing the histopathological score and increasing the regenerative score.