Straw Fractions Research Articles

NUTRITIONAL COMPOSITION OF WHEAT STRAW ENSILED WITH ADDITIVES ON DIFFERENT FERMENTATION DAYS

The proximate analysis and fiber fractions of wheat straw ensiled with urea (2.5%), poultry litter, watermelon peels and pineapple peels at 25% inclusion each for 0, 6, 12, 18, 24 and 30 fermentation days were determined. The experiment was laid out in a Factorial arrangement in a Randomized Complete Block Design (RCBD) with five treatments and 3 replications for each treatment. Wheat straw (SWS) was ensilage without additives served as the control. All the treatments were conducted in an open mouthed kilner jar which were opened following the fermentation of 0, 6, 12, 18, 24 and 30 days for sample collection and determination of CP, CF, EE, Ash, DM, NFE, ADF, NDF, Cellulose, Hemicellulose and Lignin. The results obtained shows significant difference (P<0.05) in all the additives across the fermentation days. PLWS shows highest values of CP at 30 FDs (11.07%), CF (35.98%), Ash (5.12%), ADF (35.04g/100g), NDF (55.40g/100g), Cellulose (13.06g/100g), and Lignin (12.00g/100g) while WPWS and PPWS have ranging values CP (5.99-8.50%), CF (24.16-29.87%), EE (1.51-2.03%), Ash (4.45-5.03%), ADF (23.56-29.56g/100g), NDF (35.40-45.42g/100g), Cellulose (8.48-10.59g/100g) and Lignin (7.89-9.88g/100g) across the fermentation days. Ensiling wheat straw with poultry litter (PLWS) for 30 FDs is recommended for ruminant feeding because it yields better proximate composition and fiber fractions.

Comprehensive Wheat Straw Processing with Deep Eutectic Solvent to Deliver Reducing Sugar

Pretreatment is one of the bottlenecks in the cost and energy-efficient biomass valorization. Deep eutectic solvents are potential candidates for being used to address these challenges. In this work, the deep eutectic solvent composed of choline chloride, and acetic acid was studied for its use in wheat straw fractionation. The pretreated biomass was assessed concerning the lignin and glucan content. Under optimized time and temperature conditions, defined using Doehlert matrix chemometric tool, of 3 h 47 min and 139.6 °C, the processed wheat straw contained as much as 42.5 ± 0.42 wt.% and 38.59 ± 1.26 wt.% of glucan and lignin contents, respectively. The need for biomass washing after the pretreatment with deep eutectic solvents and before the enzymatic hydrolysis step was also evaluated. The obtained enzymatic hydrolysis results, i.e., glucan to glucose yield of 27.13 ± 0.25 vs. 25.73 ± 0.08 for washed or unwashed biomass correspondingly, are equally good substrates. Fractal kinetic analysis of the data showed similar values of k and h for both glucose and xylose reactions between washed and unwashed biomass. This confirmed that biomass washing is an unnecessary step, which in turn opens room for biomass processing intensification.

Niobium-based single-atom catalyst promoted fractionation of lignocellulose in choline chloride-lactic acid deep eutectic solvent

Pretreatment is the key step to convert lignocelluloses to sustainable biofuels, biochemicals or biomaterials. In this study, a green pretreatment method based on choline chloride-lactic acid deep eutectic solvent (ChCl-LA) and niobium-based single-atom catalyst (Nb/CN) was developed for the fractionation of corn straw and further enzymatic hydrolysis of cellulose. With this strategy, significant lignin removal of 96.5 % could be achieved when corn straw was pretreated by ChCl-LA (1:2) DES over Nb/CN under 120 °C for 6 h. Enzymatic hydrolysis of the cellulose-enriched fraction (CEF) presented high glucose yield of 92.7 % and xylose yield of 67.5 %. In-depth investigations verified that the high yields of fractions and monosaccharides was attributed to the preliminary fractionation by DES and the deep fractionation by Nb/CN. Significantly, compared to other reported soluble catalysts, the synthesized single-atom catalyst displayed excellent reusability by simple filtration and enzymatic hydrolysis. The recyclability experiments showed that the combination of ChCl-LA DES and Nb/CN could be repeated at least three times for corn straw fractionation, moreover, the combination displayed remarkable feedstock adaptability.

Potential Antifungal of Merang Padi Extract (Oryza sativa) Against Fungal Growth

<div><table cellspacing="0" cellpadding="0" align="left"><tbody><tr><td align="left" valign="top"><p><em>Merang padi is a traditional medicine that comes from natural ingredients, namely plants. However, there has not been much research on the activity of rice straw extract (Oryza sativa) being explored and researched. The aim of this research was to determine the yield of extracts and the number of antifungal isolates in inhibiting fungal growth and their potential as antifungals against Candida albicans and Aspergillus fumigatus fungi. This research uses experimental research with a quantitative approach carried out through laboratory tests. The design used was a Completely Randomized Design (CRD) which was grouped into 6 treatment groups, namely 4 groups of rice straw extract and 2 control groups which were repeated for each treatment 4 times. The treatment group consisted of P1 with rice straw extract with a concentration of 20,000 ppm, P2 with rice straw extract with a concentration of 40,000 ppm, P3 with rice straw extract with a concentration of 60,000 ppm and P4 with rice straw extract with a concentration of 80,000 ppm. Meanwhile, the control group, namely P0 as a negative control, namely NaCl 0.9% and P5 as a positive control, namely Nystatin 100,000 IU. The research sample was rice straw from rice plants that had been identified and then weighed. Data analysis used one way ANOVA SPSS version 20. If there were differences then a further test was carried out, namely the Honest Significant Difference Test (BNJ) to see the differences between each treatment based on the coefficient of diversity (KK) value obtained at the 5% test level. The results of tests that have been carried out show that the n-hexane fraction of rice straw has no antifungal activity, while the ethyl acetate and n-butanol fractions have antifungal activity. The concentration of ethyl acetate in this study was greater than the other compounds.</em></p><p> </p></td></tr></tbody></table></div><p><strong><em>Keywords : Candida albicus, merang padi, mushrooms, oryza sativa</em></strong></p>

Chemical Composition And Surface Images Of Untreated And Urea-Treated Rice Straw As Inf uenced By Days After Threshing

The purpose of this study was to evaluate the chemical composition and changes in the surface structure of rice straw as affected by the number of days of exposure to the field prior to processing. A total of 24 packs of rice straw samples collected at 1 day after threshing (DAT), 4 DAT, and 7 DAT field exposure untreated and treated with urea for 10 days were analyzed for its chemical changes using proximate analysis and surface structure of the fiber compounds through scanning electron microscopy (SEM). Results (%DM basis) showed that 1DAT untreated rice straw obtained the highest dry matter (DM) concentration at 94.61%. Seven DAT untreated rice straw contain the highest amount of Ash at 20.92%. Likewise, 1 DAT and 7 DAT untreated rice straw contains higher levels of crude protein (CP) at 9.60% and 9.79%, respectively. Ether extract (EE) was observed highest in 1DAT untreated rice straw at 1.97%. Considerable breakdown of cellulose, hemicellulose, and lignin fractions of rice straw was attained in 4DAT and 7DAT untreated, and 1 to 7 DAT urea-treated rice straw (UTRS) as evident by its lower NDF, ADF, and ADL values. Changes in the surface structures of microfibers efficiently captured using SEM showed the differences in the morphology of the fibers as influenced by number of days exposed to the field prior to treatment. Therefore, in order to optimize the protocol to improve the feeding and nutritional quality of rice straw, it is suggested that the number of days of exposure to the field be considered prior to processing. Likewise, UTRS is an effective method to disintegrate the complex fibrous compounds of the cell wall of rice straw.

Experimental investigation of Co-Composting of gelatine industrial sludge combined with slaughter house waste and rice straw

<p>This work illustrates the co-composting of Gelatine Industry Sludge (GIS) combined with organic fraction of Slaughter House Waste (SHW) and Rice Straw (RS) employing 10% zeolite mixed with Enriched Nitrifying Bacteria Consortium (ENBC). Five piles of GIS will be prepared and mixed with SHW and RS at 2:1:0.5, 4:1:0.5, 6:1:0.5 and 8:1:0.5 and without GIS 0:1:0.5 (dry weight basis) served as control, while ENBC was inoculated in all piles and Windrow composted for 42 days. After complete the composting process to characterized to determine, Moisture content, Temperature pH, EC, TOC, TKN, P, K, C/N Ratio, Bacteria, Fungi, Actinomycetes as per the standard methods (Indian Standard). To find out the co-variations of the samples, and Least Significant Difference value calculated for each parameter. The reactor 4 with GIS, SHW and RS ratio 8:1:0.5 were reduced the nitrogen loss and co variations is very low. The co amendment of 8% Gelatine Industrial Sludge effectively buffered the pH between 6.5-8, while lower concentration of the Gelation Industrial Sludge was comparatively delayed the early decomposition. Therefore, our results suggested that suitable of initial sample ratio 8:1:0.5 as the best formulation for the composting of gelatine industrial sludge into value added stable product.</p>

Green and facile recycling of bauxite residue to biochar-supported iron-based composite material for hydrothermal liquefaction of municipal solid waste

Industrial and municipal wastes remain significant sources of air, soil, and water pollution, thus causing adverse climate and health impacts. EU faces challenges in developing green recycling processes and reducing GHG emissions. Innovation in green catalysis is a key driver toward the fulfilment of these goals. This study demonstrated a single-step “Green Recycling” route by which different wastes e.g., industrial and bioorganic wastes are treated to produce biochar/Fe(0) (BC-Fe(0)) material. Typically, three different biomass namely organic fraction of municipal solid waste (biopulp), wheat straw (WS), and microalgae (MA) were used as green reducing agents for reducing bauxite residue (BR). Among all biomass, the high reduction potential of amino acids present in biopulp facilitated the synthesis of BC-Fe(0). BC-Fe(0) material acted as an effective catalyst for HTL of biopulp as the results showed the highest bio-crude yield (44 wt%) at 300 °C for 30 min with 10 wt% BC-Fe(0) loading (containing 2.5 wt% Fe). Furthermore, BC-Fe(0) also assisted in-situ hydrogenation and deoxygenation of chemical compounds present in the bio-liquid product, therefore bio-crude exhibited a higher H/C ratio (1.73) and lower oxygen contents (9.78 wt%) in comparison to bio-crude obtained without catalyst. However, Raw BR and reduced BR (RED) as catalysts showed no significant effect on the yield and oxygen content of bio-crude, which confirms the high catalytic activity of Fe(0) containing BC-Fe(0). Therefore, this study demonstrates the greener path for the one-step valorization of industrial and organic wastes, as an alternative to existing chemical and high temperature-based waste recycling and catalyst synthesis technologies.

The coupling effects between acid-catalyzed hydrothermal pretreatment and acidic/alkaline deep eutectic solvent extraction for wheat straw fractionation

Mild hydrothermal pretreatment (HP) integrating with solvent extraction is a promising two-step technique to enhance the overall lignin and carbohydrate output for lignocellulose fractionation. This work comparatively assessed the coupling effect between mild HP (the first step) and the emerging acidic choline chloride-natural acid or alkaline choline hydroxide based deep eutectic solvents (DES, the second step) for wheat straw fractionation. It was shown HP with 0.3% p-toluenesulfonic acid (p-TsOH) catalyst achieved a good compromise between complete hemicellulose removal (nearly 100%) and high cellulose recovery (99.2%). While choline hydroxide based DES showed better coupling effect with HP than choline chloride-natural acid DES, corresponding to 75.6 and 31.2% lignin removal respectively. It was proposed that the alkaline DES enhanced lignocellulose swelling the lignin phenolic hydroxyl groups deprotonation and thus facilitating lignin solubilization despite of its condensation at HP. Therefore, the alkaline DES resulting cellulose-rich fraction exhibited higher potential for further processing.

Differentiated Fractionation of Various Biomass Resources by p-Toluenesulfonic Acid at Mild Conditions.

Biomass is the ideal substitute for petrochemical resources because of its renewable and abundant sources. p-Toluenesulfonic acid (p-TsOH) can effectively separate lignin from biomass under mild conditions, so it is highly expected in biomass fractionation to improve the utilization efficiency. In this study, we investigated the effect of p-TsOH differentiated fractionation of poplar sawdust, eucalyptus sawdust, and rice straw below 100 °C. According to the experimental results, upon pretreatment by p-TsOH of the three kinds of raw biomass, most of the lignin and hemicellulose of poplar sawdust and eucalyptus sawdust were removed, whereas the cellulose was retained, but most of the hemicellulose and cellulose of rice straw were kept, whereas the lignin was removed at similar conditions. The structures and compositions of pretreatment residues, lignin, and hemicellulose extracted from raw biomass were characterized by XRD, FTIR, HSQC-NMR, XPS, and SEM. The differentiated fractionation mechanism of biomass was analyzed. A better recognition and understanding of the factors affecting biomatrix opening and fractionation will allow for the identification of new pretreatment strategies that improve biomass utilization and permit the rational enzymatic hydrolysis of cellulose.

Preparation of lignin nanoparticles via ultra-fast microwave-assisted fractionation of lignocellulose using ternary deep eutectic solvents.

Lignin separation from natural lignocellulose for the preparation of lignin nanoparticles (LNPs) is often challenging owing to the recalcitrant and complex structure of lignocellulose. This paper reports a strategy for the rapid synthesis of LNPs via microwave-assisted lignocellulose fractionation using ternary deep eutectic solvents (DESs). A novel ternary DES with strong hydrogen bonding was prepared using choline chloride, oxalic acid, and lactic acid in a 1:0.5:1 ratio. Efficient fractionation of rice straw (0.5 × 2.0 cm) (RS) was realized by the ternary DES under microwave irradiation (680 W) within only 4 min, and 63.4% of lignin could be separated from the RS to prepare LNPs with a high lignin purity (86.8%), an average particle size of 48-95 nm, and a narrow size distribution. The mechanism of lignin conversion was also investigated, which revealed that dissolved lignin aggregated into LNPs via π-π stacking interactions.

Potential Antibacterials of Merang Padi Extract (Oryza sativa) on the Growth of Gram Positive and Gram Negative Bacteria

Padi straw (Oryza sativa) is one type of stem produced from padi tree trunks. Antibacterial activity testing was carried out by agar diffusion method using paper disc as a backup by observing the formation of a clear zone indicating the presence of antibacterial activity. Variations in the concentration of the padi straw extract test solution were made with a %w/v ratio between 100% pure padi straw extract and DMSO adjusted at various test concentrations, namely, 5%, 10%, 15%, 20%, and 25%. The results showed that the n-hexane fraction of padi straw did not have antibacterial activity because there was no inhibition zone or kill zone indicated by the fraction at both low and high concentrations. While the ethanol extract showed antibacterial activity at a concentration of 15%, the ethyl acetate fraction and the n-butanol fraction had antibacterial activity indicated by the presence of an inhibitory zone at a concentration of 7%. The results of the t-test on the ethanol extract of the test bacteria obtained a Tcount value of 14,687 which means it is very significant or very significantly different because Tcount is greater than Ttable (5% and 1%). This shows that the oxytetracycline antibiotic is better than the potency of the ethanol extract of straw on the test bacteria.

Microwave-assisted alkali pre-treatment medium for fractionation of rice straw and catalytic conversion to value-added 5-hydroxymethyl furfural and lignin production

In the current study, the use of microwave-assisted sodium hydroxide medium (MWSH) for pre-treatment and saccharification of rice straw to obtain sugar syrup for the production of 5-hydroxymethyl furfural (5-HMF) was investigated. The optimization of the MWSH pre-treatment was carried out using central composite methodology, resulting in a maximum reducing sugar yield of 350 mg/g of treated rice straw (TRS) and a glucose yield of 255 mg/g of TRS under the conditions of a microwave power of 681 W, a NaOH concentration of 0.54 M, and a pre-treatment time of 3 min. Additionally, the microwave assisted transformation of sugar syrup with titanium magnetic silica nanoparticle as catalyst, producing 41.1 % yield of 5-HMF from the sugar syrup after 30 min microwave irradiation at 120 °C with catalyst loading of 2.0:200 (w/v)). The structural characterization of the lignin was analysed using 1H NMR techniques, and the surface carbon (C1s spectra) and oxygen (O1s spectra) composition changes of the rice straw during pre-treatment were analysed using X-ray photoelectron spectroscopy. The rice straw based bio-refinery process which contains MWSH pretreatment followed by dehydration of sugars achieved high efficiency of 5-HMF production.

Nitric acid-potassium hydroxide fractionation of rice straw: an integrated biorefinery initiative

Abstract Pulping of rice straw was studied using mild nitric acid in mild conditions. Spent nitric acid was chosen as soil nutrient rather than followed by the liquor recovery. The rice straw was treated with 11.03% nitric acid at 90 °C for 3 h, yielded 53.09% pulp. Nitric acid treated rice straw pulp had high residual lignin and minerals. However, further treatment with 7% potassium hydroxide reduced the residual lignin and produced pulp with kappa number 20.36. The papermaking properties of the nitric acid followed by KOH treated pulp showed better quality than the nitric acid pulp. The spent nitric acid liquor was reused repeatedly, pulp yield decreased and residual lignin content increased in each step of spent nitric acid reusing. The spent nitric acid liquor and potassium hydroxide liquor-mixed together to get a neutral effluent liquor which was rich with potassium, nitrogen and biomass. The nutritional capacity of the effluent liquor was assessed by incubation with control soil. The labile form of organic carbon, nitrogen, potassium phosphorous and iron increased in the incubated soil.

Biphasic fractionation of lignocellulosic biomass based on the combined action of pretreatment severity and solvent effects on delignification

A novel method based on pretreatment severity and solvent effects on delignification, was introduced to pretreat and fractionate lignocellulose in a 2-phenoxyethanol (EPH) biphasic solvent system. The combined severity factor (CSF) was used to regulate pretreatment severity, and the relative energy difference (RED) of solvent system to lignin was used to evaluate solvent effects. The combined action of pretreatment severity and solvent effects on delignification was first investigated by the response surface regression analysis on the pretreatment of Amorpha. Accordingly, pretreatment and fractionation of Amorpha, poplar and corn straw were then conducted under the optimized conditions. Results showed that >99 % lignin was removed after pretreatment with CSF 3.7845 in a solvent system with RED 0.9371, and 42.94 %, 39.41 % and 70.90 % lignin from Amorpha, poplar and corn straw were respectively regenerated from organosolv liquor after fractionation. Finally, the regenerated products were characterized by FTIR, TG and GPC analysis.

Using rice straw fractions to develop reinforced, active PLA-starch bilayers for meat preservation

Bilayers from thermoplastic corn starch (TPS) and PLA were obtained, incorporating or not rice straw (RS) valorised fractions: active extract (es) into PLA and cellulose fibres (cf) into TPS films. The films were obtained by thermoprocessing while the bilayers were obtained by thermocompression of the different monolayers (TPS-PLA, TPScf-PLA, TPS-PLAes and TPScf-PLAes). TPS conferred oxygen barrier capacity to the laminates, which was improved by the cf incorporation. The extract slightly reduced the PLA resistance but improved their oxygen barrier capacity. The tensile and barrier properties of the bilayers revealed changes in the performance of each layer associated with the interlayer compound migration. The TPScf-PLAes bags exhibited noticeable antioxidant capacity when used in meat packaging and reduced microbial counts throughout cold storage. Therefore, these bilayers have considerable potential to extend the shelf-life of meat samples, preserving their quality and safety for longer, while using RS fractions permits its valorisation.

Ammonia fiber expansion-assisted deep eutectic solvent treatment for wheat straw fraction separation and bioconversion

In this study, an ammonia fiber expansion (AFEX)-assisted deep eutectic solvent (DES) pretreatment method was developed for the rapid separation of wheat straw fractions, which reduced the pretreatment time for DES and improved the pretreatment efficiency. This study describes the feasibility of the AFEX-assisted DES pretreatment in terms of both progressive and parallel relationships and analyzes the subsequent enzymatic effect in generating glucose from cellulose. Ammonia fiber expansion-assisted DES one-pot pretreatment at 120 °C, for 1.5 h resulted in an enzymatic efficiency of 98.0 ± 3.1 %. Moreover, the enzyme efficiency remained greater than 85 % after three recovery cycle experiments. The comparison between regenerated-lignin (d-lignin) and alkaline-lignin showed that regenerated lignin has a lower molecular weight and belongs to para-hydroxy-phenyl-guaiacyl-syringyl (H-G-S) type lignin. This study developed is a green and efficient pretreatment process with great potential in the separation and utilization of biomass fractions.

Quantifying Nutrient and Economic Consequences of Residue Loss from Harvest Weed Seed Control

Harvest weed seed control (HWSC) methods destroy, remove, or concentrate weed seeds collected during harvest. Depending on the method of HWSC, chaff and straw fractions may also be destroyed, removed, or concentrated. Observations at soybean (Glycine max (L.) Merr.) harvest in this study estimated the distribution of aboveground biomass between seed, straw, and chaff fractions and the nutrient composition of straw and chaff. Measurements were combined to predict nutrient consequences of HWSC, which have not been documented. The average harvest index of soybean was 0.57:1. Soybean biomass that enters the combine partitions into 7.25 ± 0.37% chaff, 36.05 ± 1.2% straw, and 56.7 ± 1.2% seed. Chaff and straw residues equal 13.4% and 68.5% of the seed weight, respectively. In a soybean crop yielding 3368 kg ha−1 (50 bu a−1), chaff yields 9.4, 0.8, 5.0, and 0.6 kg ha−1 and straw 31.6, 2.1, 1.1, and 2.0 kg ha−1 of N, P, K, and S, respectively. Using 5-year average fertilizer prices ending in 2021, the cost to replace chaff, straw, and the combination of both residues is USD 1.58, USD 5.88, and USD 7.46, respectively. These results give insight into the nutrient consequences and replacement costs of HWSC.

Extraction of the wheat straw hemicellulose fraction assisted by commercial endo-xylanases. Role of the accessory enzyme activities

Wheat straw is a highly promising raw material for bio-refinery strategies. Most of the literature related to lignocellulose fractionation focuses on cellulose purification and hemicellulose solubilization. Pre-treatments for hemicellulose solubilization without the formation of undesired products usually reach low extraction yields, which leaves an important hemicellulose fraction unused. In this work, we propose a mild process for the efficient extraction of the hemicellulose fraction of wheat straw assisted by partial enzymatic hydrolysis with three commercial endo-xylanase cocktails. A first step with alkali at 40 ºC helped to disrupt the lignocellulosic structure and removed 19% of lignin while maintaining most of the hemicellulose in the solid. The enzymatic step enabled reaching extraction yields of 59.8%, 51.9%, and 42.5% with Ultraflo L, Pentopan mono conc, and Shearzyme 500L, respectively. We also discuss the catalytic properties of each endo-xylanase, in particular, their adscription to the GH10 or GH11 glycosyl hydrolase family, and the relevant role of accessory enzymes.

Oxidation Characteristics of Water Soluble Fractions of Agro-Stalks with Focus on Function of Reactive Inorganics

In order to deepen the understanding of the thermochemical behavior of reactive inorganics, which play an important role in slagging and fouling during combustion of agro-stalks, the oxidation behavior of the water-soluble fraction of corn stover, wheat straw and rice straw was examined using a simultaneous thermogravimetric analyzer. The oxidation characteristics were discussed in combination with elemental analysis of water-soluble fractions. Results showed that reactive inorganics elements account for 30–40% in water-soluble fractions of the three agro-stalks and carbon was oxidized at two separate stages. Four stages were found during oxidation of water-soluble fractions – (1) devolatilisation of organics (100‒400 °C); (2) oxidation of char (400–650 °C); (3) oxidation of char with melting of salts or decomposition of carbonate (650–800 °C); (4) vaporization of KCl (800–1000 °C). This work provides a base study for an optimized design of combustion for agro-stalks and pharmaceutical waste.

Improvement of lignocellulosic pretreatment efficiency by combined chemo - Mechanical pretreatment for energy consumption reduction and biofuel production

A pretreatment process is a preliminary stage to alter the recalcitrant structure of lignocellulosic biomass for enhancing its digestibility. An evaluation of the energy consumption and the environmental restrictions of the pretreatment step is required to develop a feasible eco-friendly process for industrial implementation. In this study, a combined alkaline-mechanical fractionation of rice straw for fermentable sugar (FS) production was developed with the objectives of i) evaluating energy efficiency and ii) addressing the sustainability of technology. The maximum fermentable sugar production was 0.54 kg/kg rice straw with an enzymatic hydrolysis efficiency of 97.34%. Furthermore, the results indicated that the pretreatment by 5% NaOH followed by mechanical size reduction provided the highest energy efficiency of 0.35 kg of FS/kWh with 94.44% glucose recovery, which also provided the lowest waste generation of less than 0.1 kg of waste/kg FS. These experimental results suggest that the combined alkaline-mechanical fractionation could effectively increase total FS yields and could be a promising pretreatment technology for an eco-friendly and sustainable pretreatment process integrated into a biorefinery platform.