This paper investigates the tracking problem of fractional-order multi-agent systems. Both the order and parameters of the leader are unknown. Firstly, based on the positive system approach, the asymptotically stable criteria for incommensurate linear fractional-order systems are derived. Secondly, the models of incommensurate heterogeneous multi-agent systems are introduced. To cope with incommensurate and heterogeneous situations among followers and the leader, radial basis function neural networks (RBFNNs) and a discontinuous control method are used. Thirdly, the consensus criteria are derived by using the Vector Lyapunov Function method. Finally, a numerical example is presented to illustrate the effectiveness of the proposed theoretical method.