The prognosis for patients with cervical cancer (CC) is strongly correlated with the Ki-67 proliferation index (PI). However, the Ki-67 PI obtained through biopsy has certain limitations. The non-Gaussian distribution diffusion model of magnetic resonance imaging (MRI) may play an important role in characterizing tissue heterogeneity. At present, there are limited data available concerning the prediction of Ki-67 PI using models based on histogram features of non-Gaussian diffusion distribution. This study aimed to determine whether preoperative histogram features from multiple non-Gaussian models of diffusion-weighted imaging can predict the Ki-67 PI in patients with CC. Our cross-sectional prospective study recruited a total of 53 patients suspected of having CC who underwent 3.0-T MRI at Sun Yat-sen Memorial Hospital of Sun Yat-sen University between January 2022 and January 2023. Fifteen b values (0-4,000 s/mm2) were used for diffusion-weighted imaging. A total of nine parameters from four non-Gaussian diffusion-weighted imaging models, including continuous-time random walk (CTRW), diffusion kurtosis imaging (DKI), fractional order calculus (FROC), and intravoxel incoherent motion (IVIM), were used. Whole-tumor volumetric histogram analysis of these parameters was then obtained. In logistic regression, significant histogram characteristics were statistically examined across two groups to build the final prediction model. To assess diagnostic parameters of the proposed model in the diagnosis of the Ki-67 PI, along with the sensitivity, specificity, and diagnostic accuracy of these various parameters from the four models, receiver operating feature analysis was applied. Among the 53 patients (55.3±9.6 years, ranging from 23 to 79 years) included in the study, 15 had a Ki-67 PI ≤50% and 38 had a Ki-67 PI >50%. Univariable analysis determined that 12 histogram features were statistically different between the two groups. In multivariable logistic regression, we ultimately selected 6 histogram features to construct the final prediction model, with CTRW_α_10th percentile [odds ratio (OR) =0.955; 95% confidence interval (CI): 0.92-0.99; P=0.019], CTRW_α_robust mean absolute deviation (OR =0.893; 95% CI: 0.81-0.99; P=0.028), and CTRW_α_uniformity (OR =0.000, 95% CI: 0.00-0.90, P=0.047) being the independent predictive variables. The area under the curve of the combined prediction model was 0.845 (95% CI: 0.74-0.95), with a sensitivity of 78.9% (95% CI: 0.63-0.90), a specificity of 86.7% (95% CI: 0.60-0.98), an accuracy of 81.1% (95% CI: 0.68-0.91), a positive predictive value of 93.8% (95% CI: 0.79-0.99), and a negative predictive value of 61.9% (95% CI: 0.38-0.82). The histogram features of multiple non-Gaussian diffusion-weighted imaging can help to predict the Ki-67 PI of CC, providing a new method for the noninvasive evaluation of critical biological features of CC.
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