New developments for continuous-time system identification with fractional order models are proposed in the CRONE toolbox (version 2.0). Fully compatible since Matlab® 2020a, it includes time-domain identification algorithms for estimating continuous-time models directly from sampled data. In this new version 2.0, the reliability has been improved thanks to a complete revision of the classes and their unitary tests. Thanks to this new programming, the options arguments of the proposed functions have been simplified and updated. In order to help a new user, a tutorial has been completely revised as the CRONE_demos command and to handle the new options. A Guided User Interface (GUI) is now proposed, as the CroneIdentification application so that a user, familiar with the SystemIdentification GUI, can easily handle the system identification methods for preprocessing data, defining a model structure and estimating as well the coefficients and the differentiation orders.