ABSTRACT Introduction: The academic community has demonstrated the link of athletic performance in track and field with critical factors of sports training, including the psychology of competition. Recently, research has been verified that athletic performance is also related to altitude, weather, ambiance, and the timing of the competition. There are no studies to provide theoretical and practical training experience in the regional environment of Ganzhou. Objective: This paper analyzes the influence of altitude training in professional performance athletes. Concurrently, analysis of the physiological and biochemical indicators of the athletes is performed. Methods: Several long-distance runners had an 8-week altitude training. Analyzed the physiological and biochemical indicators of the athletes before and after the training. At the same time, perform mathematical-statistical analysis on the performance of long-distance runners before and after training. Results: After altitude training, hemoglobin and red blood cells showed a significant decrease (P<0.01). After training, athletes’ performance improved considerably (P<0.01). Conclusion: Training at different altitudes may promote an ascending curve in the athletes’ performance until a plateau in the fourth week of training. Evidence Level II; Therapeutic Studies - Investigating the result.