Fourth Experimental Group Research Articles

Effectiveness of the use of enzyme preparation Hemicell® HT for growing young goslings

Agricultural waterfowl have certain biological characteristics: intensive metabolism and rapid growth, early maturity and signifcant reproduction potential, high body temperature, and resistance to many infectious diseases. The article presents the results of an experimental study of the effect of the exogenous enzyme preparation Hemicell® HT (1 kg contains the active ingredient β-mannase - 160x106 units) on the productive qualities of young goslings of Shadryna and Italian breeds. To achieve this goal, four experimental groups of goslings were formed: Group 1 - control, 2, 3, 4 - experimental, which were fed the enzyme preparation Hemicell® HT as part of a complete feed mixture - 150 g for the second experimental group, 250 g for the third and 310 g for the fourth experimental group per 1 tonne of fodder. It was found that the introduction of the exogenous enzyme preparation Hemicell® in the above doses into the diet of young geese signifcantly affects the growth rate of young geese and metabolic processes in their bodies. If at the end of the frst decade the most positive effect on the body was found in low and high dos- age of the enzyme preparation (birds of the 2nd and 4th groups), as a result of which, compared to the analogues of the 1st control group, the difference in the average daily gain was 3.4 and 2.8 g (p<0.001), then the average dosage of the enzyme preparation in the analogues of the 3rd experimental group provided a difference of only 1.1 g (p<0.01). However, by the end of the frst rearing period (up to 21 days), the highest average daily weight gain was observed in geese of the 3rd experimental group compared to the analogues of the control, 2nd and 4th experimental groups. Thus, while the average daily weight gain in geese of the 1st control group was 43.1 g, this fgure was 2.6 g higher in the 2nd group (p<0.01), 5.1 g in the 3rd group and 3.6 g in the 4th group (p<0.001). This pattern was maintained until the end of the experiment, with signifcant differences in goslings of the 3rd experimental group compared to the control: at the age of 31 days - by 9.7 g (p<0.01), 41 days - by 10.1 g (p<0.05), 66 days - by 9.2 g (p<0.05). In geese of the 2nd and 4th groups, the average daily weight gain was higher than in the control group, but did not have significant differences during the same age period. As a result, in general, the average daily weight gain of goslings of the 1st control group was 59.9 g, in the 2nd experimental group it was 3.1 g (p<0.01), or 5.2%, in the 3rd - 6.6 g (p<0.001), or 11%, and in the 4th group - 4.2 g (p<0.001), or 7.0%, compared to the same indicator of the control. The use of the enzyme preparation had a positive effect on the goslings' organism, which made it possible to realise the genetic potential of goslings' productivity at a higher level in all experimental groups, but the highest growth rate was observed in the 3rd experimental group of geese. Key words: geese, Shadryna breed, Italian breed, enzyme preparation, Hemicell® HT, weight gain, live weight.

Exploring Diverse Teaching Models for Enhancing Nursing Students' English Language Proficiency: A Blended Learning Perspective

The present study examines the efficacy of blended learning models in enhancing nursing students’ proficiency in the English language. It is challenging to come across comparative studies that assess the effectiveness of blended learning models in advancing the English language skills of nurses. However, the experimental study includes five groups, and each group has gone through five different instructional models of teaching. The first experimental group received instruction through the rotation model; the second experimental group received instruction through the flex model; the third experimental group received instruction through the self-blend model; the fourth experimental group received instruction through the enriched-virtual model; and the fifth group which served as control group received instruction through the communicative language teaching approach. A total of one hundred and fifty participants were selected through random sampling from Aligarh Muslim University, India. The data were gathered using pre- and post-tests administered before and after the intervention of these models using the standardized general English test (TOFEL). The repeated measure ANOVA revealed that each group manifested significant advancement in nurses' English language skills. Nevertheless, the rotation model demonstrated superior performance in enhancing nurses' English language skills in comparison to the other groups. The findings of the study hold pedagogical significance for those who are responsible for designing curricula, developing training programs for future nurses, producing materials, and all other persons who are involved in nursing education.

Effect of metal nanoparticles usage on oxidative stress indicators and endotoxemia parameters under DMH-induced carcinogenesis

One of the properties of nanoparticles is their ability to correct manifestations of oxidative stress and endotoxemia, which are critical factors in cancer development. Therefore, the work aimed to investigate the effect of the usage of Au/Ag/Fe nanoparticles on oxidative stress indicators and endotoxemia parameters in experimental colon carcinogenesis. The study was performed on 90 white male rats kept in standard vivarium conditions. The division into groups: I – intact animals; II – intact animals with 21 days NPs administration; III – animals injected with N,N-dimethylhydrazine dihydrochloride for 30 weeks; ІV – animals to which Au/Ag/Fe nanoparticles were intragastrically administered daily for 21 days after induced adenocarcinoma. According­ to our results, the concentration of oxidative stress indicators significantly increases under DMH-induced carcinogenesis conditions. It was established that the 21-day intragastric administration of NP Au/Ag/Fe composition caused a significant (P < 0.001) decrease in the concentration of TBARS in the blood serum by 1.33 times, in the content of diene and triene conjugates by 1.63 and 1.98 times, respectively compared to the third experimental group. The introduction of NPs in the fourth experimental group reduces the concentration of the Schiff bases by 1.34 times (P < 0.001), decreases the content of POMP370 and POMP430 by 1.25 (P < 0.001) and 1.37 times (P < 0.001), respectively, compared to the third experimental group. We also observed the reduction of endotoxemia levels in a fourth experimental animal group based on a significant decrease in MMM indexis and EII percentage. Keywords: Au/Ag/Fe nanoparticles, endotoxemia, induced colon carcinogenesis, N‚N-dimethylhydrazine dihydrochloride, oxidative stress

Mannan oligosaccharides as a prebiotic for laying hens: effects on fertility, hatchability, productive performance, and immunity.

This experiment examined how adding mannan-oligosaccharides (MOS) to the diet affected fertility, hatching rates, egg production, carcass characteristics, cost-effectiveness, and immune function in laying hens. One hundred and twenty Mandarah chickens (30 hens and 3 roosters per group) were randomly chosen between 34 and 50wk old and divided into four groups. The first group was the control group, which was given just the basal diet. The basal diet was given to the second, third, and fourth experimental groups along with three different levels of MOS (0.1, 0.2, and 0.5g/kg diet, respectively). Results found that hens fed MOS at various levels laid eggs at a significantly higher rate, enhanced egg number, egg mass and feed conversion ratio than the control group (P < 0.05). MOS seemed to improve carcass quality. The best results for egg quality (Haugh unit) and testosterone levels were seen with a dose of 0.5g/kg of MOS compared to the control birds (P < 0.05). All MOS levels led to higher estradiol-17β (E2) levels and better economic efficiency (EE). MOS also improved the hens' immune systems as compared to the control group. Hens-fed MOS had significantly greater levels of antibodies against Influenza viruses (H9N2) and Infectious Bronchitis Virus (P < 0.05). Also, the spleen and thymus gland, both crucial immune system components, were slightly larger (P < 0.05). It's important to note that fertility rates, hatchability, and embryo mortality rates remained similar across all groups. So, our findings suggest that incorporating MOS into the birds' diet enhances their productivity, strengthens their immune system, improves EE, and contributes to the overall health of the hens.

Использование жиров различного происхождения в комбикормах для бройлеров

Purpose. Determination of the effectiveness of using fats of various origins in compound feed for broiler chickens. Materials and Мethods. A scientific and economic experiment was carried out on 4 groups of broiler chickens of the Cobb-500 cross at the Verkhnevolzhskaya Poultry Farm (Russia, Tver region). The poultry-rearing period was 37 days. Broilers in the control group received the main diet according to the growing phases; for chickens in the experimental groups, part of the sunflower oil in the diet was replaced with animal feed fat "Olisa": in group 2 – 0.5% in CCF Rost, 1.0% in Finish-1 and Finish- 2; in group 3 – 1.0% in CCF Rost, 1.5% in Finish-1 and Finish-2; in group 4 – 1.5% in CCF Rost, 2.0% in Finish-1 and Finish-2. The dynamics of live weight were recorded by weekly control weighing of broiler chickens. The safety of the poultry population was determined by counting dead birds. The absolute and average daily gain in live weight, the cost of feed per 1 kg of gain in live weight, the productivity index and other indicators were also determined during the growing process. Results. The introduction of 1.5% animal feed fat “Olisa” into the compound feed instead of part of the sunflower oil in CCF Rost and 2.0% in Finish-1 and Finish-2 contributed to an increase in both the average daily and absolute gain in live weight. The latter indicator was higher in broiler chickens of the fourth experimental group by 8.6% compared to the control group. Poultry from the same group had a higher meat yield per 1 m2 of floor area. Their productivity index was higher than in the control group by 6.6%. At the same time, the safety of poultry population in all groups participating in the experiment was high. Conclusion. Replacing part of the sunflower oil in feed with animal fat in the optimal dosage contributes to obtain high live weight, daily gain, safety of broiler chickens and productivity index.

Effects of repeated intravenous doses of tranexamic acid on closed tibial fracture healing: Experimental study based on the rat model.

The aim of this study was to assess the effects of tranexamic acid on fracture healing in the rat tibia closed fracture model. Sixty-four male Sprague-Dawley rats were included in this study, where closed fracture and intramedullary nailing were performed on their right tibial diaphyses. They were divided into 2 main groups, the experimental group, which was given weekly tranexamic acid injections, and the control group, which received no additional treatment. Eight rats from each group were sacrificed and evaluated for fracture healing at the first experimental group and control group, second experimental group and control group, third experimental group and control group, and fourth experimental group and control group weeks. Fracture healing was radiologically assessed according to the "Spencer Index" and "Lane and Sandhu Scoring System," and histologically evaluated according to the scoring system devised by Huo et al. Results: According to the Spencer Index, the mean union score was statistically significantly higher in the E3 group than in the third con- trol group (P = .014). Furthermore, the mean union score was statistically significantly higher in the fourth experimental group compared to the fourth control group (P=.047). According to the Lane and Sandhu Scoring System, only the mean union scores of the E3-4 groups were statistically significantly higher than the mean union scores of the C3-4 groups (P=.048). There was no histological difference between groups in terms of union, according to the criteria defined by Huo et al (P > .05). This study showed us that repeated intravenous administrations of tranexamic acid had no negative effect on fracture heal- ing in the rat tibia fracture model. Although tranexamic acid demonstrated better radiological healing in the late period, it had no effect on histological union.

Comparative analysis of body weight gain in laboratory animals with different routes of administration of Lcarnitine

In recent years, several studies have been published that suggest that L-carnitine supplementation may affect weight in some animal species and humans, and may also affect the body composition of growing animals. Currently, only a few results are available on the effects of L-carnitine on weight loss and body composition in animals. Therefore, the purpose of this study was to find out whether injections of L-carnitine can influence the dynamics of the weight of laboratory animals. Toxicity studies were conducted on outbred rats in October 2021 in the vivarium of the St. Petersburg State University of Veterinary Medicine. The study involved females weighing 190-210 grams, purchased from the Federal State Unitary Enterprise “Nursery of Laboratory Animals “RAPPOLOVO”. When studying subchronic toxicity after subcutaneous and intramuscular administration, L-carnitine was administered at 2 dose levels. Doses were determined based on the results of the acute toxicity experiment: 1/5 and 1/10 of the maximum tolerated dose. The first experimental group (n=10) received the drug intramuscularly at a dose of 0.08 mg/kg (1/5 of 2000 mg/kg). The second experimental group (n=10) received the drug intramuscularly at a dose of 0.04 mg/kg (1/10 of 2000 mg/kg). The third experimental group received subcutaneous L -carnitine at a dose of 0.08 mg/kg (1/5 of 2000 mg/kg), the fourth experimental group received subcutaneous L-carnitine at a dose of 1/10 of the maximum tolerated (2000 mg/kg body weight). Group 5 was the control group (n=10) and received sodium chloride 0.09% intramuscularly at a dose of 1/5 of 2000 mg/kg, group 6 was the control group and received sodium chloride 0.09% subcutaneously at a dose of 1/5 of 2000 mg /kg. As a result of studies of subchronic toxicity on laboratory animals of the drug L-carnitine for veterinary use with subcutaneous and intramuscular administration, it was established that a dosage of 1/5 of the maximum tolerated, and a dosage of 1/10 of the maximum tolerated, does not cause changes in the dynamics of body weight animals.

Lactational performance and nutrients digestibility response of dairy buffaloes fed diets supplemented with probiotic (Streptococcus spp.) and fibrolytic enzymes.

The current study was conducted to explore the productive performance and health status of lactating buffaloes fed diets supplemented with probiotic and/or fibrolytic enzymes. Forty multiparous lactating Egyptian buffaloes (body weight 451 ± 8.5 kg) were equally assigned to four experimental groups: (1) the first group fed control diet, (2) second experimental group fed control diet plus 4 g of probiotic/kg dry matter (DM) (probiotic), (3) third experimental group fed control diet plus 4 g of fibrolytic enzymes/kg DM (enzymes) and (4) fourth experimental group fed control diet plus 2 g of probiotic + 2 g fibrolytic enzymes/kg DM (Mix), The experiment was extended for 63 days. Nutrients digestibility was estimated, daily milk yield was recordedand milk samples were analyzed for total solids, fat protein, lactoseand ash. Blood serum samples were analyzed for glucose, total protein, albumin, urea-N, aspartate transaminase, alanine transaminaseand cholesterol concentrations. Results showed that adding probiotic and/or fibrolytic enzymes improved nutrients digestibility (p < 0.05). The probiotic, enzymesand mix groups did not affect (p > 0.05) concentrations of serum total protein, albumin (A), globulin (G), albumin/globulin (A/G) ratioand urea-N concentrations. An improvement in daily milk yield (p < 0.0001) and energy-corrected milk(p = 0.0146) were observed with the probiotic and mix groups compared with the control. In conclusion, this study suggests that supplementing lactating buffaloes'diets with probiotic alone or in combination with fibrolytic enzymes would improve their productive performance without adversely impacting their health.

The use of medicinal plants in the diets of lactating cows

In studies conducted on the territory of a farm belonging to the Novgorod Research Institute on Ayrshire cows during lactation, the use of plants with medicinal properties – crushed leaves of stinging nettle and fruits of red mountain ash for forcing the excretion of cesium and potassium radionuclides through the gastrointestinal tract and kidneys was studied. Seven groups of cows were selected for the research (n = 10 in each). For animals of the first three groups, in addition to the main feed, crushed nettle leaves were used (5, 10 and 30 g/head/day), for the next three groups, crushed rowan berries (5, 10 and 30 g/head/day) were used. For animals of the control group, a set of feeds was used, established and adjusted for this farm. It was found that when using herbal supplements in the diet, the concentration of caesium-137 in the urine of animals of all experimental groups significantly exceeded the control indicators. The content of potassium-40 in the urine of the fourth experimental group exceeds the control by 43%, in the rest the indicator is stable. In the feces of cows of the experimental groups, there is a significant increase in the concentration of both caesium-137 (from 31 to 136% relative to the control) and potassium-40 (from 24 to 166%). Accordingly, the release of radionuclides by the gastrointestinal tract and kidneys in the experimental groups is more active, relative to the indicators of the control groups. In some cases, more radionuclides were excreted than were ingested with food.

The content of microelements in the blood of young bulls after correction of diets with deficient microelements

Micronutrients, or micronutrients, are an essential component of the diet of farm animals and play a key role in their health and performance. The importance of trace elements in physiological processes and ensuring the normal functioning of the animal body is highlighted. Micronutrients affect growth, fertility, the state of the immune system, the health of the skin and coat, and metabolism. This scientific study emphasizes the importance of a balanced consumption of trace elements in the diet of farm animals to achieve high-performance indicators and ensure their overall health. It is essential to consider that the deficiency or excess of trace elements in the diet of animals can lead to various problems with health and productivity. Therefore, accurate, balanced nutrition and control over the content of trace elements are critical aspects of animal husbandry. It is essential to consider that different species of animals can show different vulnerability to trace elements, and therefore, it is necessary to ensure the correct balance of trace elements in the diet of each species of animal to preserve their health and productivity. This article describes the feeding of deficient trace elements and their chelated compounds (Fe, Cu, Mn, Co, Zn). The research aimed to investigate the content of microelements (Cu, Pb, Mn, Co, Zn, Cd, Ni, Fe) in the blood of young cattle after correcting diets with deficient microelements. The obtained results show that the level of trace elements increased slightly in the animals of the 2nd, 3rd, and especially the 4th experimental group. Thus, at the end of the experiment, the content of trace elements in the cattle of the experimental groups was higher than in the animals of the control group. The highest amount of trace elements in the blood was observed in the fourth experimental group, which was fed chelated compounds of trace elements (methionates) in the following doses: CuMet(0.05), MnMet(0.1), ZnMet(0.1), FeMet(0.05), CoMet(0.03) mg/kg body weight.

The effect of chelating compounds on the meat qualities of beef

An important aspect is the problem of balanced micronutrient nutrition in diets, which arises from insufficient macro- and micronutrients in soil and feed. Some of these elements act as components of biologically active compounds and regulate various metabolic processes. A lack or excess of these elements can lead to significant metabolic disorders in the animal's body and decreased productivity. The ratio imbalance occurs when a violation of metabolism accompanies fattening young cattle concerning mineral substances, a decrease in the productivity and stability of animals, and deterioration of veterinary and sanitary indicators and meat quality. The research aimed to reveal the effect of enriching rations with deficient microelements in combination with chelating compounds (methionates) on the productivity of experimental cattle. The use of trace elements and their chelated compounds, such as methionates and other biologically active substances, has advantages. This helps to reduce the assimilation of heavy metals and radionuclides from contaminated feed and water. Chelated complexes of microelements easily penetrate through cell membranes, which allows you to have a targeted effect on the metabolism of substances and energy and correct the deficiency of microelements in the corresponding biogeochemical zones. With the addition of trace element supplements and their chelated compounds, the slaughter yield of bulls of the 2nd, third, and fourth experimental groups increased by 0.15, 1.48, and 2.26 kg, respectively, compared to the control group. The yield of high-grade meat increased by 9.18, 11.02, and 13.9 kg, respectively, for the control. Also, the first-grade muscle tissue yield increased by 11.35, 12.95, and 15.1 kg compared to the control. Correction of the rations of experimental animals with deficient trace elements in the form of salts and their chelated compounds (methionates) contributed to improving beef's physical and chemical composition and nutritional value. Thus, we conclude that the fourth experimental group, which was fed microelements in the form of chelated compounds (methionates), has the best indicators in terms of all parameters, the second and third experimental groups, which were fed inorganic salts of deficient microelements, have slightly lower indicators, respectively. In the future, we will use chelating compounds of deficient trace elements to improve beef's physiological and morphological indicators.

Influence of dietary mannan-oligosaccharides supplementation on hematological characteristics, blood biochemical parameters, immune response and histological state of laying hens

This study aimed to determine the influence of dietary mannan-oligosaccharides (MOS) on the immune system, hematological traits, blood biochemical parameters, and histological state of laying hens. At 34 wk of age, The Mandarah chicken strain's 120 laying hens and 12 cocks were divided into 4 groups, each with 30 hens and 3 cocks. The first group performed as a control group, which nourished on a basal diet. The second, third, and fourth experimental groups received 0.1, 0.2, and 0.5 g/kg of MOS and a base diet, respectively. Birds obtained MOS at numerous doses significantly (P ˂ 0.05) raised serum levels of immunoglobulin Y (IgY), immunoglobulin M (IgM), and avian influenza (AI) antibodies compared to control birds. Furthermore, adding MOS at a level of 0.1 g/kg diet significantly improved the immune response of the control group. Additionally, compared to the control group, treated birds with MOS at various dosages did not significantly enhance hematological parameters such as red blood cells (RBCs), white blood cells (WBCs), hemoglobin, and hematocrit. Compared to control birds, birds fed MOS at all levels exhibited considerably lower serum cholesterol, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) values. Also, compared to other treated birds, MOS-treated birds displayed improved histological examination of the small intestine, isthmus, and testis compared to the control group, particularly in birds fed MOS at 0.1 and 0.2 g/kg diet. It could be concluded that using MOS at 0.1 or 2 g/kg diet can successfully improve the physiological performance and overall health of laying hens.

Restoration of the morphofunctional state of rats lungs with experimental fibrosis through transplanted stem cells

Aim. To investigate the therapeutic effect of allogeneic mesenchymal stem cells (MSC) derived from bone marrow on the restoration experimentally damaged lung tissue in rats with induced pulmonary fibrosis. Methods. Female Wistar rats were utilized in the study. Pulmonary fibrosis was induced experimentally in the animals by administering bleomycin hydrochloride via transthoracic injection during a 45-day preparatory period. At the end of this period, all animals exhibited clinically manifested symptoms of pulmonary fibrosis. To assess the changes in the organism due to pulmonary fibrosis, blood samples, bronchoalveolar lavage samples, and lung tissue were randomly collected from five affected animals. The remaining animals were divided into four experimental groups, each consisting of five animals. In the first experimental group, allogeneic MSC were administered intrathoracically. In the second experimental group, allogeneic MSC were administered intravenously. The third experimental group received conventional treatment using medication, while the fourth experimental group (control) received placebo intrathoracically, without any active agents. Additionally, a separate fifth experimental group comprised intact (healthy) animals. The stem cells used in the experiments were obtained from the bone marrow samples of young, clinically healthy donor rats’ tibia, humerus, or femur (Mazurkevych et al, 2014). Throughout the 45-day experiment, the animals in all experimental groups were monitored using clinical examination indices. At the end of the testing period, the animals were eutha- nized, and blood, bronchoalveolar lavage, and lung tissue samples were collected for laboratory analyses. The analysis of bronchoalveolar lavage involved microscopic examination of the collected fluid to determine cell composition, while hematological analysis encompassed the quantification of erythrocyte and leukocyte counts. Histological examination of lung tissue samples involved microscopic analysis of the lung tissue histostructure. Results. Following the implementation of the proposed treatment methods, it was observed on day 45 of the experiment that transplanted allogeneic MSC facilitated an increased activity in the restoration of pathologically altered lung parenchyma. The effectiveness of this process varied depending on the method of MSC application. Notably, animals in the first experimental group exhibited the absence of symptoms such as coughing and hypoxia by day 45. Furthermore, a significant decrease was observed in the indices of erythrocyte count to 7.18 ± 0.05 t/l (р &lt; 0.001) and total leukocyte count to 10.6 ± 0.92 g/l (р &lt; 0.05). Histological analysis revealed a complete restoration of lung tissue structure, reaching 90 % when compared to the control group. In the second experimental group, a significant decrease was noted in the total leukocyte count to 11.32 ± 0.48 g/l (р &lt; 0.01) and erythrocyte count to 6.87 ± 0.18 t/l (р &lt; 0.001). Histologically, the regenerative processes exhibited lower activity, reaching 70–80 % compared to the animals in the first group. Fibrous areas were observed but of smaller size than those in the conventional treatment group. In the third experimental group, despite the normalization of laboratory testing indices, namely a decrease in erythrocyte count to 7.62 ± 0.11 t/l (р &lt; 0.001) and leukocyte count to 9.46 ± 0.54 g/l (р &lt; 0.001), occasional fibrosis areas and thicker alveolar walls were still present in the histological structure of the lungs. The fibrosis areas decreased by only 20–30 %. Conclusions. Our findings demonstrate that the application of mesenchymal stem cells is a more effective approach to cell therapy for the restoration of pathologically altered lung tissue in rats with experimentally induced pulmonary fibrosis compared to conventional treatment. Traditional medication-based treatment over the 45-day experiment led to the normalization of clinical indices and laboratory tests but did not result in complete restoration of the damaged lung tissue structure. These results underscore the advantages of employing allogeneic mesenchymal stem cells in the therapy of pulmonary fi brosis, indicating their potential for further investigation and clinical application.

Immune response, hematological traits, biochemical blood parameters, and histological status of laying hens influenced by dietary chitosan-oligosaccharides

This experiment aimed to examine the effect of chitosan-oligosaccharides (COS) supplementation in laying hens' diets affected their immune response, hematological characteristics, blood biochemical parameters, and histological status. At the age of 34 wk, 200 laying hens and 20 cocks of the Mandarah chicken strain were allotted into four groups, each consisting of 50 hens and five cocks. The first group acted as a control group, fed on a basal diet. The second, third, and fourth experimental groups each received 0.1, 0.2, and 0.5 g/kg of COS in addition to a base diet. Birds received COS at various dosages had significantly (P ˂ 0.05) increased serum concentration of immunoglobulins, avian influenza, and Newcastle disease antibodies compared with the control birds. Moreover, adding COS at level 0.2 g/kg diet insignificantly enhanced immune response than the rest of the treatment groups. Also, treated birds with COS at different levels had insignificantly improved hematological parameters such as red blood cells, white blood cells, hemoglobin and hematocrit compared to the control group. Birds fed COS at all levels had significantly decreased serum cholesterol, triglycerides, Ca++ and alanine aminotransferase concentrations compared with control birds. In addition, compared to the control group, chitosan-treated birds showed enhanced histological examination of the small intestine, isthmus, and testis, notably in birds given COS at 0.1 g/kg diet compared to other treated birds. Cocks fed COS at all levels improved testicular tissues and increased the number and diameter of seminiferous tubules compared with control birds Morphological examination of the ileum showed increased villi number, height, and crypt depth. It is possible to conclude that laying hens' physiological performance and general health can be effectively improved by using chitosan at 0.1 or 2 g/kg diet levels enhanced immune response.

Navigating Teacher’s Display and Referential Questions to Enhance Learners’ Speaking Accuracy: A Case of Explicit and Implicit Corrective Feedback

Corrective feedback with its potential role in oral interaction, and teacher&amp;rsquo;s questions with the capacity to engage the learners in conversational activities led to the investigation of their roles in speaking accuracy of EFL learners. Teacher&amp;rsquo;s display and referential questions were employed along with explicit (explicit correction, metalinguistic clue, elicitation) and implicit (conversational recast, repetition, clarification request) corrective feedback to create opportunities for the learners to participate in interaction, to modify their errors, and to produce accurate output. Therefore,112 learners who attended 10 intact classes of 15 session terms in one control and four experimental groups were homogenized through administering a PET. In all groups, accuracy was focused while learners were engaged in conversational activities. In the first and second experimental groups teacher&amp;rsquo;s display questions were implemented followed by the provision of explicit and implicit feedback types. However, the third and fourth experimental groups were asked to answer teacher&amp;rsquo;s referential questions who received explicit and implicit corrective feedback respectively. To measure the learners&amp;rsquo; speaking accuracy, both pre and posttests of speaking were recorded and transcribed to estimate the percentage of error free clause. An analysis of covariance indicated that in learners&amp;rsquo; speaking accuracy; both teacher&amp;rsquo;s display and referential questions with either explicit or implicit feedback types were significantly effective; there were no significant differences between the effectiveness of teacher&amp;rsquo;s display and referential questions with explicit corrective feedback; and teacher&amp;rsquo;s referential questions were significantly more effective than display questions with implicit feedback types. The substantial enhancement of EFL learners&amp;rsquo; speaking accuracy bears testimony that in interactional view, communicative behavior resulting from the questioning and corrective feedback paves the way for a higher level of accurate output.

Restructuring of erythropoiesis and immune status in hypotrophic calves with comorbid pathologies based on the use of aminoseleferon-B

The article presents the results of comparative therapeutic efficacy of a complex biogenic drug aminoseleferon-B based on recombinant interferons on Holstein calves with hypotrophy and comorbid pathology (anemia, immunodeficiency) in the neonatal period. According to the principle of pairanalogues, four groups of 6 individuals (hypotopic calves with comorbid pathology) were formed and the fifth – 6 normotrophic calves. The calves of the first experimental group were administered aminoseleton, the calves of the second experimental group - biferon-B, the calves of the third experimental group - the drug aminoseleferon-B. The drugs were administered subcutaneously, three times on days 1, 3 and 30 at a dose of 1 ml per 10 kg of animal weight. The calves of the fourth experimental group - negative control and the calves of the fifth group - positive control, the studied drugs were not used. The use of aminoseleferon-B leads to an earlier stabilization (on day 7 of our studies) of the morphological and immunological blood composition, whereas in the comparison groups, the studied indicators were optimized on day 30. In the animals of the 3rd experimental group, the level of erythrocytes increased by 9.3% and 6.4% compared to those in hypotrophic calves, a similar pattern was noted in the amount of total hemoglobin - increased by 13.7% and 7.7%. Humoral and cellular links of nonspecific protection were restored in the calves of the 3rd experimental group, due to normalization of serum compliment, lysozyme and bacterial activity, phagocytic index and number. The use of aminoseleferon-B in the treatment of polymorbid pathology (anemia, immunodeficiency) in calves with hypotrophy has a pathogenetically mediated mechanism of action and a pronounced anti-anemic and immunomodulatory effect that together improves the state of the calves body during early neonatal adaptation.

The Relationship Between the Type of Dimensions (3D, 2D) and Mental Interaction (High, Low) in the Augmented Reality Environment and its Effects on Learning

The research aims to reveal the relationship between the type of dimensions (3D, 2D) and the type of mental interaction (high and low) and its impact on students’ knowledge of the types of wireless networks and their components in the course “Wireless Communications and Mobile Security” based on their satisfaction with this learning, and their academic achievement. The research sample is 144 students in the second year, fourth level of the cybersecurity diploma, and they were divided into four experimental groups. The first experimental group studies with books augmented with three-dimensional models with high mental interaction, and the second experimental group studies with books augmented with three-dimensional models with low mental interaction. The third experimental group is taught with books augmented with two-dimensional models with high mental interaction, and finally, the fourth experimental group is taught with virtual books with two-dimensional models with low mental interaction. The research results that there is an interaction between the type of dimensions in augmented reality books and the mental interaction (high, low) in identifying the types of wireless networks and their components, where the high mental interaction groups excelled, regardless of the type of dimensions (3D, 2D) in the achievement test, and there was clear satisfaction for the students of the groups that study with 3D models compared to the 2D groups.

Impact of Supplementing Phytobiotics as a Substitute for Antibiotics in Broiler Chicken Feed on Growth Performance, Nutrient Digestibility, and Biochemical Parameters.

To determine the level of application and the effectiveness of the use of plant feed additives from sweet chestnut wood extract (Castanea Sativa Mill) in the diet of Cobb-500 cross broiler chickens, four groups were formed via the balanced groups method. The chickens in the experimental groups were supplemented with sweet chestnut wood extract in the main diet rather than a feed antibiotic at an amount of 500 g per ton of compound starter feed and 250 g per ton of grower and finisher in the second experimental group; 650 g per ton of compound starter feed and 325 g per ton of grower and finisher in the third experimental group; and 800 g per ton of compound starter feed and 400 g per ton of grower and finisher in the fourth experimental group. Supplementation with phytobiotics led to an increase in the digestibility of the dry matter in the second and third groups compared to the first experimental group. Furthermore, broiler chickens supplemented with a medium dose of phytobiotics revealed a significant difference in both crude protein and fiber when compared to the second experimental group (91.95% and 12.11% vs. 88.98% and 10.07%, respectively). The preslaughter weight of the birds in the phytobiotics supplemented groups was higher than in those fed with the lowest dosage of phytobiotic by 5.47%, and the difference was significant. There were no significant differences in terms of the blood biochemical parameters between the groups. In summary, the inclusion of plant feed additives from sweet chestnut wood extract as a substitute for an antibacterial drug in the diet of broiler chickens did not deteriorate the blood biochemical parameters and improved the intensity of the nutrient digestion process. As a result, it enhanced the quality indicators of the broiler carcass during the entire growth period.

CLINICAL AND EXPERIMENTAL REASONING OF THE USE OF COLLAPAN-L AND THYMOGEN, AND THEIR COMBINATIONS IN THE TREATMENT OF FRACTURES OF TUBULAR BONES IN WHITE RATS

This paper presents the results of the use of collapan-L and thymogen, as well as their combinations, on the processes of reparative osteogenesis in experimental femur fractures in rats. It was found that their use significantly reduced the negative impact of the fracture on the main markers of bone metabolism - calcium, phosphorus, alkaline phosphatase. The most significant changes were recorded in the fourth group, where we used the combined treatment of drugs, an immunostimulator immediately after surgery for a course of 5 days. The results of X-ray and histological studies also indicate more active processes of osteogenesis and reparative regeneration of bone tissue in the fourth experimental group. Thus, on the 45th day after surgery, the callus was homogeneous, the fracture line was barely visible. In addition, a higher proportion of bone and a low content of connective tissue at the fracture site were noted compared to other groups of animals. Thus, in comparison to the control group of animals, the content of bone tissue in the fourth experimental group is higher by 8.86%, and the connective tissue is lower by 46.06%. The results obtained in determining the strength of bone to mechanical stress also indicate that the most effective method for stimulating reparative osteogenesis in fractures is the combined use of collapan-L and thymogen, starting immediately after surgery for five days. Thus, on the 45th day after surgery, the strength of the femur in the fourth group reached 53.02% of the intact level, which was 9.39% higher than the control values.

Pengaruh Konseling Kelompok Dengan Teknik Self-Management Terhadap Prokrastinasi Akademik

This experimental study was conducted to determine whether there is an effect of self-management on academic procrastination. This study uses a true experimental research design with the type of Solomon Four Group Design. Participants in this study were 60 university students in Surabaya who were given a pretest to determine whether these students had a high level of procrastination. The measuring instrument used in this study is the Tuckman Procrastonation Scale (TPS). The results of the pretest showed that 40 students had a high level of procrastination, then the 40 students were randomly assigned (random assignment) into two groups. There were 20 students in the control group and 20 students in the experimental group. In accordance with the type of research used, each group was divided into four groups and received different treatment. The first and third experimental groups will receive group counseling using self-management techniques and will be given a pretest and posttest, while the second and fourth experimental groups will only receive counseling using self-management and pretest techniques. The first and third control groups will receive a pretest and posttest, while the second and fourth control groups will not receive treatment and will only receive a posttest. The results of this study indicate that the null hypothesis of this study is accepted, that is, there is no influence between self-management techniques and academic procrastination.