In this paper we report on the development of an electronic DNA sensor probe assembly using barium strontium titanate (BST) applied by pulsed laser deposition in conjunction with optical spectroscopy and impedance spectroscopy methods. To verify the binding of the probe to the BST, Fourier transform infrared (FTIR) spectroscopy and attenuated total reflectance (ATR) techniques were employed. FTIR and ATR were employed to verify the hybridization of the probe to an oligonucleotide target. In addition to the FTIR technique, direct capacitance and impedance spectroscopy measurements were used to verify the ability of the probe binding to the ceramic substrate and also the hybridization of the target DNA strand to the probe DNA strand. The proposed design has the advantages of label-free detection and large detectable capacitance changes upon hybridization, and does not require the application of a gold electrode layer.