A stepped-frequency (SF) radar waveform generation method based on Fourier domain mode-locking (FDML) period-one laser dynamics is proposed and demonstrated. By fast controlling the optical injection strength of a semiconductor laser through electro-optical modulation, a broadband SF signal is generated. By further introducing an optoelectronic feedback loop with its round trip time delay matched with the temporal period of the SF signal, FDML is enabled through which the frequency stability, accuracy, and in-band signal-to-noise ratio are greatly improved. In the experiment, SF signals with a bandwidth of 6 GHz (12-18 GHz) and a frequency step of 150 MHz are generated. By comparing the qualities of signals generated with and without FDML, advantages of the proposed SF signal generation method are verified. Based on the proposed SF signal generation method, high-resolution inverse synthetic aperture radar (ISAR) imaging is also demonstrated, in which the 2D imaging resolution reaches 2.6 cm × 3.82 cm.