To overcome the shortcomings of traditional concrete structures such as long construction cycle, high construction cost and poor durability, seven composite beams with a UHPC stay-in-place formwork (UCB) and one reinforced concrete (RC) beam were designed. An experimental study was conducted to investigate the flexural behavior of composite beams under the static load. The research parameters include formwork thickness, reinforcement rate, and formwork surface treatment. The results revealed that roughening the surface of UHPC formwork has a positive impact on enhancing the integrity of composite beams. The utilization of UHPC stay-in-place formwork can effectively improve the stiffness, cracking load, and peak load of members. Compared with that of the RC beam, the cracking load and peak load of composite beams increased by 63% ~ 103% and 6% ~ 15%, respectively, and the yielding stiffness increased by 23% ~ 41%. Different from the RC beam, new cracks occurred on one side of the initial crack's end and continued to extend upwards rather than along the original crack. The existence of a significant quantity of tiny cracks in composite beams effectively slowed down the increase in the width of pre-existing cracks in the early stage of loading. Due to the multi-crack pattern exhibited by the UHPC formwork, the strain concentration of the steel bars was effectively mitigated. When subjected to the same load level, the strain of the longitudinal steel bars in composite beams was smaller than that of the RC beam. The formula for calculating the flexural bearing capacity of composite beams is established through theoretical analysis. In addition, the numerical model of the composite beam is established by the finite element method (FEM). The influence of the contact method of the UHPC-NC interface on the flexural performance of the composite beam is explored. The supplementary analysis of the parametric study of the reinforcement rate and the UHPC formwork thickness is carried out. When using ABAQUS for the numerical analysis of the composite beam, it is appropriate to adopt the cohesion model or the Tie model for the UHPC-NC interface, and it is not appropriate to use the Coulomb friction model.