Dominant Form Research Articles

Heritage, tourism theming, and intercultural processes: The cases of Oaxaca, Mexico, and Malaga, Spain

ABSTRACT Theming is a common tool in urban tourist destinations. It allows the recognition of some type of brand that makes a destination more competitive. In this work, the themings of Oaxaca, Mexico, and Malaga, Spain, are compared. Malaga offers an international and avantgarde cultural experience, while Oaxaca is known for the ancestral traditions that still prevail in the city. It is possible to define a highly touristified spatial core in both cities. Additionally, both cities have themed their historic centers, focusing on historical and intangible elements. We focus on how some tourist systems reproduce ethnocentric, colonial, or other forms of domination while branding their cities for consumption. Even within systems where such an asymmetry cannot be clearly defined, the selection of discourse might affect social self-attitude and identity on varying levels. Although Oaxaca looks firmly at its past, at the same time, such success magnifies tradition and hinders modernity. Meanwhile, Malaga looks to the future but loses some of its traditions at a very high cost.

The Complexity of Being A20: From Biological Functions to Genetic Associations.

A20, encoded by TNFAIP3, is a critical negative regulator of immune activation. A20 is a ubiquitin editing enzyme with multiple domains, each of which mediates or stabilizes a key ubiquitin modification. A20 targets diverse proteins that are involved in pleiotropic immunologic pathways. The complexity of A20-mediated immunomodulation is illustrated by the varied effects of A20 deletion in different cell types and disease models. Clinically, the importance of A20 is highlighted by its extensive associations with human disease. A20 germline variants are associated with a wide range of inflammatory diseases, while somatic mutations promote development of B cell lymphomas. More recently, the discovery of A20 haploinsufficiency (HA20) has provided real world evidence for the role of A20 in immune cell function. Originally described as an autosomal dominant form of Behcet's disease, HA20 is now considered a complex inborn error of immunity with a broad spectrum of immunologic and clinical phenotypes.

Unlearning possessive belonging: reading in relation with Indigenous science fiction

ABSTRACT The work of Indigenous thinkers as writers of, and commentators on, Indigenous science fiction, calls for the unmaking of settler futurities in ways that assert Indigenous belonging in all times. In this paper, we, as science fiction nerds with settler and white privilege in so-called Australia, try to respond to calls from Indigenous science fiction to sit with, and contribute to, a decentring of presumed settler futures. Together, we read speculative fiction through the work of Aboriginal author, Ambelin Kwaymullina’s, the Tribe Series. As we do so, we are learning to read-in-relation to the invitations of Indigenous science fiction as it asserts Indigenous agency, refuses dominant forms of citizenship and calls on readers to respond to our diverse positions and places. As we engage with Kwaymullina’s work, we seek to engage with Aboriginal-led futurities and dwell with times not invested in the continuance of settler institutions and possessive forms of belonging.

MISSENSE MUTATIONS IN THE IRAK1 GENE ARE ASSOCIATED WITH AN INCREASED RISK OF SYSTEMIC LUPUS ERYTHEMATOSUS

Single nucleotide polymorphisms (SNPs) are the most common form of genetic variation in humans. Missense SNPs can change protein structure and function. This study aimed to determine missense SNPs of the IRAK1 gene that can affect the amino acid sequence and lead to changes in protein structure and function, as well as their relationship as a risk factor for SLE. In this in silico method, several bioinformatics tools have been used to identify missense SNPs, including their properties and impacts, as well as their interaction networks with proteins. The tools used were PolyPhenv2, SIFT, PhD-SNP, PROVEAN, SNAP, Panthers, I-Mutant 3.0, and GeneMania. Four missense SNPs, rs11465830, rs1059702, rs1059703, and 10127175, were obtained from the NCBI SNP database. The SIFT test results showed that all the SNPs were tolerant. In the test results obtained using PolyPhen, the four SNPs were benign. The results of the probe test indicated that the four SNPs were neutral. When tested with SNAP, one SNP was neutral, and three others had an impact. In the PhD-SNP test, all SNPs were neutral. In the panther test, all SNPs were benign. The I-mutant assay showed that the four SNPs could decrease protein stability. Tests with GeneMania have reported that most interactions between genes were between IRAK1 and MYD88, and physical interactions were the most dominant form of interaction. Conclusion. rs10127175, rs11465830, rs1059702, and rs1059703 are missense SNPs in IRAK1, which can disrupt protein stability and be a risk factor for SLE. Keywords: IRAK1, SNP, Missense, Systemic Lupus Erythematosus

Steroid hormone signaling synchronizes cell migration machinery, adhesion and polarity to direct collective movement.

Migratory cells - either individually or in cohesive groups - are critical for spatiotemporally regulated processes such as embryonic development and wound healing. Their dysregulation is the underlying cause of formidable health problems such as congenital abnormalities and metastatic cancers. Border cell behavior during Drosophila oogenesis provides an effective model to study temporally regulated, collective cell migration in vivo. Developmental timing in flies is primarily controlled by the steroid hormone ecdysone, which acts through a well-conserved, nuclear hormone receptor complex. Ecdysone signaling determines the timing of border cell migration, but the molecular mechanisms governing this remain obscure. We found that border cell clusters expressing a dominant-negative form of ecdysone receptor extended ineffective protrusions. Additionally, these clusters had aberrant spatial distributions of E-cadherin (E-cad), apical domain markers and activated myosin that did not overlap. Remediating their expression or activity individually in clusters mutant for ecdysone signaling did not restore proper migration. We propose that ecdysone signaling synchronizes the functional distribution of E-cadherin, atypical protein kinase C (aPKC), Discs large (Dlg1) and activated myosin post-transcriptionally to coordinate adhesion, polarity and contractility and temporally control collective cell migration.

Memeing back at misogyny: emerging meme-feminism, visual tactics, and aesthetic world-building on Iranian social media

ABSTRACT In this article, I examine the emergence of “meme-feminism” on Iranian social media, which adopts innovative tactics to combat gendered hate online. I propose the concept of “memeing back” at misogyny and numerous gendered inequalities as not only a unique feminist tactic but also a political and satirical world-building practice. With a detailed analysis of theorizations of (digital) feminist activism and mediated visual humor, I demonstrate how the adoption of memes can, through anonymity and visual community-building, extend beyond dominant forms of popular feminism to combat the pervasion of anti-feminism on Iranian social media. I further unpack how this tactic mobilizes feminist memes by utilizing shared aesthetic visualization, a feminist politics of exposure, and visual nagging as embodied and mediated interventions to critique socioeconomic inequalities impacting women-identifying individuals in Iran. On this basis, I argue for the significance of online memes and the “memeing back” tactic in advancing feminist demands under suppressive, violent, and capitalist states.

Challenging power relations in food systems governance: A conversation about moving from inclusion to decolonization

This reflective essay explores power relations, with a particular focus on racialization, that flow through dominant forms of food systems govern­ance, with an aim to create more participatory gov­ernance models. Four of the authors asked a group of five scholars, activists, and practi­tioners (also authors) who identify as Black, Indige­nous or People of Color (BIPOC) to discuss dur­ing a conference session issues of Indigenous food sovereignty, decolonization, Whiteness, and inclu­sivity in food systems governance. This paper pre­sents and analyzes the content of the session, part of the 2021 Global Food Governance Conference. We reflect on common themes from the session and put forth recommendations: encouraging greater inclusion in existing forms of food systems governance, achieving decolonization through cre­ating diverse new governance models, and address­ing the deeper power structures that underpin the dominant food system itself. We also suggest a research agenda, with the “what” of the agenda unfolding from a process of agenda development that centers BIPOC scholarship. The frameworks offered by the panelists are a starting point, as more work is needed to move towards decolo­nizing food systems governance research. Finally, a collaborative agenda must attend to the inextricable links of food systems governance to other funda­mental issues, such as the emerging field of planetary health.

Convergence of dominant forms of investment capital in the development of socio-economic systems

The object of this study is the dominant forms of investment capital and their convergence according to the stages of development of socio-economic systems. Any evolution has certain stages. The development of socio-economic systems is no exception. All processes and phenomena in the world, including development, occur cyclically and are interconnected with the stages of natural, social, and economic processes. According to the theory of economic cycles, such phenomena as the development of the socio-economic system, its crisis and innovations have a close relationship. Industrial revolutions became a vivid example of changes in the socio-economic development of complex systems. One of the main sources of financing for each stage of the industrial revolution is investment capital, which can be part of industrial, private, financial, banking, or intellectual capital. Depending on the signs of social relations, social system, etc. can take their forms. Each of the stages of the industrial revolution is preceded by appropriate conditions that are associated with certain historical conditions at a specific moment in time. Considering the dominant forms of investment capital according to the stages of the industrial revolution, the reasons for their change should be noted. The process of convergence of investment capital is considered here. One of the indicators that characterizes the efficiency of investment capital is its mobility or immobility. A number of macroeconomic factors affect the mobility of investment capital. Considering the world structure of direct foreign investments, the main directions of their utilization have been indicated. The model of the convergence of the dominant forms of investment capital in the logistics system is presented in accordance with the stage of the industrial revolution and the features that accompany it

Proteasome localization and activity in pig brain and in vivo small molecule screening for activators.

Loss of proteasome function, proteinopathy, and proteotoxicity may cause neurodegeneration across the human lifespan in several forms of brain injury and disease. Drugs that activate brain proteasomes in vivo could thus have a broad therapeutic impact in neurology. Using pigs, a clinically relevant large animal with a functionally compartmental gyrencephalic cerebral cortex, we evaluated the localization and biochemical activity of brain proteasomes and tested the ability of small molecules to activate brain proteasomes. By Western blotting, proteasome protein subunit PSMB5 and PSMA3 levels were similar in different pig brain regions. Immunohistochemistry for PSMB5 showed localization in the cytoplasm (diffuse and particulate) and nucleus (cytoplasm < nucleus). Some PSMB5 immunoreactivity was colocalized with mitochondrial (voltage-gated anion channel and cyclophilin D) and cell death (Aven) proteins in the neuronal soma and neuropil in the neocortex of pig and human brains. In the nucleus, PSMB5 immunoreactivity was diffuse, particulate, and clustered, including perinucleolar decorations. By fluorogenic assay, proteasome chymotrypsin-like activities (CTL) in crude tissue soluble fractions were generally similar within eight different pig brain regions. Proteasome CTL activity in the hippocampus was correlated with activity in nasal mucosa biopsies. In pilot analyses of subcellular fractions of pig cerebral cortex, proteasome CTL activity was highest in the cytosol and then ~50% lower in nuclear fractions; ~15-20% of total CTL activity was in pure mitochondrial fractions. With in-gel activity assay, 26S-singly and -doubly capped proteasomes were the dominant forms in the pig cerebral cortex. With a novel in situ histochemical activity assay, MG132-inhibitable proteasome CTL activity was localized to the neuropil, as a mosaic, and to cell bodies, nuclei, and centrosome-like perinuclear satellites. In piglets treated intravenously with pyrazolone derivative and chlorpromazine over 24 h, brain proteasome CTL activity was modestly increased. This study shows that the proteasome in the pig brain has relative regional uniformity, prominent nuclear and perinuclear presence with catalytic activity, a mitochondrial association with activity, 26S-single cap dominance, and indications from small molecule systemic administration of pyrazolone derivative and chlorpromazine that brain proteasome function appears safely activable.

AUP1 Regulates the Endoplasmic Reticulum-Associated Degradation and Polyubiquitination of NKCC2.

Inactivating mutations of kidney Na-K-2Cl cotransporter NKCC2 lead to antenatal Bartter syndrome (BS) type 1, a life-threatening salt-losing tubulopathy. We previously reported that this serious inherited renal disease is linked to the endoplasmic reticulum-associated degradation (ERAD) pathway. The purpose of this work is to characterize further the ERAD machinery of NKCC2. Here, we report the identification of ancient ubiquitous protein 1 (AUP1) as a novel interactor of NKCC2 ER-resident form in renal cells. AUP1 is also an interactor of the ER lectin OS9, a key player in the ERAD of NKCC2. Similar to OS9, AUP1 co-expression decreased the amount of total NKCC2 protein by enhancing the ER retention and associated protein degradation of the cotransporter. Blocking the ERAD pathway with the proteasome inhibitor MG132 or the α-mannosidase inhibitor kifunensine fully abolished the AUP1 effect on NKCC2. Importantly, AUP1 knock-down or inhibition by overexpressing its dominant negative form strikingly decreased NKCC2 polyubiquitination and increased the protein level of the cotransporter. Interestingly, AUP1 co-expression produced a more profound impact on NKCC2 folding mutants. Moreover, AUP1 also interacted with the related kidney cotransporter NCC and downregulated its expression, strongly indicating that AUP1 is a common regulator of sodium-dependent chloride cotransporters. In conclusion, our data reveal the presence of an AUP1-mediated pathway enhancing the polyubiquitination and ERAD of NKCC2. The characterization and selective regulation of specific ERAD constituents of NKCC2 and its pathogenic mutants could open new avenues in the therapeutic strategies for type 1 BS treatment.

Genetic Causes of Familial Adenomatous Polyposis (FAP), Risk Factors and Clinical Outcomes

Introduction: Familial adenomatous polyposis (FAP) is a hereditary disorder that is the most common colon cancer syndrome in an autosomal dominant form. At first, germline mutations in the APC gene (adenomatous polyposis) were identified as the main genetic factor causing FAP, but during subsequent studies, the human MutY (MYH) gene with an autosomal recessive pattern was also identified as the factor causing this disease, which is commonly referred to as MutYH-dependent apoptosis (MAP). FAP and MAP present with an early onset of hundreds of adenomatous polyps in the colon, at a median age of 35–40 years, and are associated with a significantly increased risk of colon cancer (CRC). In some other patients, gastrointestinal polyps, congenital hypertrophy in the retinal pigment epithelium, desmid tumors, and extracolonic malignancies are seen. The common treatment method in patients is through endoscopic and surgical methods. However, the patients with FAP and their relatives should receive appropriate genetic counseling. The purpose of this review article was to describe and investigate the clinical aspects and genetic diseases of FAP and MAP. For this purpose, the latest articles related to FAP genetic disease were selected by searching Google Scholar and PubMed sources.&#x0D; Conclusion: Despite the progress made in discovering the molecular mechanisms of FAP, its genetic factors are still not fully understood. A deeper understanding of the molecular biology and genetics of this disease can lead to healthy therapies that can be used to rezone intestinal polyps and neoplastic malignancies and be a new target for future treatment.&#x0D;

Quality parameters and lipid composition of oil extracted from spent coffee grounds: A promising alternative to vegetable oils used for consumption and cosmetic purposes

AbstractCoffee grounds are the most commonly generated daily waste in the world, raising numerous questions regarding their proper management and their environmental impact. The present study focuses on the physical and chemical characterization of oil extracted from spent coffee grounds (SCG), with the aim of valorizing this by‐product as a potential source for oil production. The results obtained from the analysis of SCG oil were also compared to those of some edible oils (soybean, corn, and sunflower oils) and cosmetic oils (lentisc, sweet almond, and castor oils). The results revealed that spent coffee waste yielded an important amount of oil, specifically 17.19%, as compared to corn (4.31%) and soybean (19.52%) seeds. Regarding the fatty acids composition of SCG oil, eight components were identified, with a high percentage of polyunsaturated fatty acids accounting for more than 45% of total fatty acids. The phytosterols composition of SCG oil revealed the presence of seven components, with β‐sitosterol being the predominant one. The tocopherol analysis showed that SCG oil contained only α and β isomers, with β‐tocopherol being the dominant form at over 68%. These findings accentuate the potential of spent coffee waste as a promising alternative for oil production and open up new applications for SCG oil, such as in the food industry, pharmaceutical applications, and cosmetics.Practical Application: Given the significant increase in the demand for vegetable oils, the oil industry has recently begun to explore new plant matrices, particularly those having low costs. SCG are the most commonly generated daily by‐product in the world. The present investigation aims to characterize and valorize the oil extracted from SCG. The obtained results revealed the potential of SCG as a promising alternative for oil production. Consequently, valorizing spent coffee waste as a natural by‐product for oil production can be an ideal solution to address numerous issues related to its proper management and its environmental impact. Hence, the oil industry can promote spent coffee waste, cheapest by‐products, as a promising alternative for oil production. Moreover, the present investigation represents a commendable model of the circular economy since the remaining waste obtained after extracting lipids from the coffee grounds waste can be used as natural fertilizer rich in protein for plant development.

Chemical Profile of Cold-Pressed Beech Nut (Fagus sylvatica L.) Oil

The objective of this study was to assess the chemical characteristics of cold-pressed beech nut oil. The nuts, gathered from the forest, comprised 25.35% water, 13.19% oil, and 19.40% protein. The predominant fatty acid was linoleic acid (40.5%), followed by oleic acid (35.0%) and gondoic acid (7.7%). All four tocopherols were present in the oil, with γ-tocopherol being the dominant form at 99.38 mg per 100 g of oil. The total sterol content was 2708.73 mg per kg of oil, with β-sitosterol constituting 80.5% of all sterols. The main characteristics of the oil included its relatively high tocopherol and gondoic acid content, a dominant oleic–linoleic fatty acid profile, and elevated levels of carotenoids.

Cultural Tariffing: Appropriation and the Right to Cross Cultural Boundaries

Why are some acts of cultural boundary-crossing considered permissible whereas others are repudiated as cultural appropriation? We argue that perceptions of cultural appropriation formed in response to the emergence of cultural omnivorousness as a dominant form of high-status consumption, making boundary-crossing a source of cultural capital. Consequently, the right to adopt a practice from a culture that is not one’s own is determined on the basis of the costs and benefits one is presumed to accrue. People express disapproval at boundary-crossing if they believe it devalues or extracts value at the expense of the target culture. We call this process cultural tariffing. We test our theory in a between-subject experimental design, demonstrating that individuals who enjoy a privileged social position, as inferred from their social identity or socioeconomic status, have less normative latitude to cross cultural boundaries. This is explained by perceptions that these actors are either devaluing or exploiting the target culture. While symbolic boundaries and cultural distinction theories are inconsistent with our results, we find that Americans who are disenchanted about group-based social mobility are the most likely to be outraged by cultural boundary-crossing. Cultural tariffing, we therefore posit, is a form of symbolic redistribution.

VPS35 and retromer dysfunction in Parkinson's disease.

The vacuolar protein sorting 35 ortholog (VPS35) gene encodes a core component of the retromer complex essential for the endosomal sorting and recycling of transmembrane cargo. Endo-lysosomal pathway deficits are suggested to play a role in the pathogenesis of neurodegenerative diseases, including Parkinson's disease (PD). Mutations in VPS35 cause a late-onset, autosomal dominant form of PD, with a single missense mutation (D620N) shown to segregate with disease in PD families. Understanding how the PD-linked D620N mutation causes retromer dysfunction will provide valuable insight into the pathophysiology of PD and may advance the identification of therapeutics. D620N VPS35 can induce LRRK2 hyperactivation and impair endosomal recruitment of the WASH complex but is also linked to mitochondrial and autophagy-lysosomal pathway dysfunction and altered neurotransmitter receptor transport. The clinical similarities between VPS35-linked PD and sporadic PD suggest that defects observed in cellular and animal models with the D620N VPS35 mutation may provide valuable insights into sporadic disease. In this review, we highlight the current knowledge surrounding VPS35 and its role in retromer dysfunction in PD. We provide a critical discussion of the mechanisms implicated in VPS35-mediated neurodegeneration in PD, as well as the interplay between VPS35 and other PD-linked gene products. This article is part of a discussion meeting issue 'Understanding the endo-lysosomal network in neurodegeneration'.

SARS-CoV-2 evolution during prolonged infection in immunocompromised patients.

SARS-CoV-2 is responsible for a global pandemic, driven in part by the emergence of new viral variants. Where do these new variants come from? One model is that long-term viral persistence in infected individuals allows for viral evolution in response to host pressures, resulting in viruses more likely to replicate efficiently in humans. In this study, we characterize replication in several hospitalized and long-term infected individuals, documenting efficient pathways of viral evolution.

Is infrastructure a critical aspect of self-organized entrepreneurial activity?

The limitation of capitalist principles and ethics has long been an issue for self-organized enterprises. Even though capitalist modes of labor organization, administration, and management are expelled from the workplace, issues of growth, investment, production, and circulation remain exposed to dominant forms of production. Nevertheless, communities of solidarity, along with spatial and techno-material settings, constitute the infrastructure of commoning practices in those endeavors. We compare the cases of the factory occupation movement in Argentina and the occupation of the Vio.Me. factory in Greece (2010), where a shift to self-organization emerged in the context of the Argentinian and Greek economic crises. We focus on struggles revolving around the use and ownership of spatial-material infrastructure and the production of use-values, highlighting the emancipatory potential of self-organization. Space and objects appear as a contested terrain, while at the same time, material settings are transformed, through the collectives’ participation in networks of distribution and solidarity.

Governance of Nemberala Coastal Natural Resources in Rote District Ndao

This study focuses on the governance of non-extractive natural resources on the Nemberala coast. More specifically, this study aims to trace the access mechanism and power relations of foreign capitalists and the government in the development of resorts, villas and cafes in the Nemberala coastal area, Rote Ndao Regency as a means of supporting tourism and what the implications are for local communities. This study uses two access mechanism approaches, Nency Peluso's Access theory (1992) and Nanang's (2021) elements of political analysis of natural resources as units of analysis. This study uses a single case study strategy with a qualitative inductive research approach. This study found that discourse and material power can change the composition of power and values ​​in an area in the form of domination as an exercise of power. Apart from that, this study found that material power operates at a closed spatial level so that in the land acquisition process on the Nemberala coast unconstitutional practices occur. The study of natural resource governance shows that there are three key actors in the governance of Nemberala's coastal natural resources, namely the government, foreign capitalists and land-owning communities. On the other hand, capitalization, privatization and commodification arise due to the globalization of the tourism market or the internationalization of tourism. Placing tourism in the capitalist space means that natural resource management will discuss who gets what, where it gets it, and how it gets it.

Novel homozygous CARD11 variants in two patients with combined immunodeficiency and atopic skin disease

BackgroundCaspase recruitment domain family, member 11 (CARD11) is an important protein which plays a fundamental role in the activation of NF-κβ pathway in lymphocytes. CARD11 deficiency can be inherited in either autosomal dominant or autosomal recessive forms and present with different phenotypes including combined immunodeficiency, atopic dermatitis, and other variable manifestations. The present report describes clinical phenotypes and immunological defects of two unrelated patients with missense homozygous variants in CARD11 presenting with combined immunodeficiency (CID) and atopic skin disease resembling that reported in dominant negative CARD11 deficiency. The patients underwent next generation sequencing, immunophenotyping of T and B subsets by flow cytometry, T cell stimulation, and evaluation of CARD11 expression.ResultsBoth patients had features suggesting CID including repeated pneumoniae with ICU admissions, chronic diarrhea, and itchy atopic skin disease. Patient-1 has homozygous missense variant in the C terminal domain (c.2839G > A, p.Glu947Lys), and patient-2 has homozygous variant in the inhibitory domain (c.1073C > G, p.Pro568Arg). Both have profound defects in Tregs with normal recent thymic emigrants, memory, and naïve CD4+ T cells. However, in response to stimulation, T cells failed to upregulate the expression of CD25. CARD11 expression by flow cytometry was decreased rather than abolished as previously described in patients with autosomal recessive CARD11 deficiency. B cells showed marked deficiency of switched memory and increase in transitional B cells.ConclusionMissense variants causing CARD11 deficiency may affect the protein function rather than the expression and can result in a phenotype combining the atopic skin disease and the features of CID.

Re-theorising namûs beyond ‘honour’: self-making, feminist agency and global epistemic justice

Namûs describes a ‘way of life’ integral to Kurdish sociality and to the sense of self for many Kurds who live it in a plurality of ways. Constituting a form of power over the subject which can potentially take the form of domination, namûs is also a social relation of care and power between subjects and is integral to its subject's ethical relationship of self-to-self and processes of self-making. Post-Enlightenment and liberal frameworks of ‘modern’ selfhood, however, have tended to render namûs equivalent to ‘honour’ and ‘honour-based violence’ (‘HBV’). Through this act of mistranslation, a life with namûs is constructed as violent, unworthy, racially inferior and harmful to women. Building upon multi-sited ethnographic fieldwork conducted in North Kurdistan, Turkey and Denmark, this article originally theorises namûs as a practice of ethical self-making that is epistemic, dignified and agentic in all its complexities. Women living with and through namûs actively work to cultivate this way of being, thereby interrupting the epistemic authority of liberal feminism. Namûs, this article argues, cannot be understood through blanket explanations of ‘crime’, ‘oppression’ and ‘patriarchy’, as the discourse on ‘honour’ would suggest. Breaking away from these injurious portrayals is, therefore, vital to realise global epistemological justice.