Forest In South-east Brazil Research Articles

Consumption of fruits of Dimorphandra jorgei M.F.Silva by Tapirus terrestris (Linnaeus, 1758) in a Remnant of Atlantic Forest in Southeast Brazil

The Fabaceae family holds great ecological and economic importance. Some species contribute to increased nitrogen levels in the soil (association with mycorrhizae), benefiting the natural regeneration process. Other species have high therapeutic value, while another can be used for ornamentation and urbanization. This study reports the presence of seeds from a tree species in the Fabaceae family, Dimorphandra jorgei M.F.Silva, in the fecal sample of South American tapir, Tapirus terrestris (Linnaeus, 1758), in a remnant of the Atlantic Forest in Southeastern Brazil. The fecal sample was found on an unpaved road, between the forest edge and an area undergoing reforestation with native species. Our record corroborates that D. jorgei can be an important supplementary food item in the tapir’s diet in the region, with its consumption linked to dry periods. The fruits of D. jorgei are not morphologically attractive to vertebrate fauna in general, highlighting the importance of tapirs in the dispersal of this and other species with similar fruits. It is also emphasized that seed dispersal contributes to the maintenance and regeneration of vegetation, serving as an Ecosystem Service of Support.

Combining mechanical control and tree planting to restore montane Atlantic forests dominated by the Neotropical bracken (Pteridium arachnoideum)

Climate and land-use changes have driven the dominance of native herbaceous plants in degraded tropical forests, leading to losses in biodiversity and ecosystem services. However, controlling these super-dominant species to promote forest regeneration is often ineffective and may favour undesirable species. Native clonal ferns from the Pteridium genus often dominate degraded tropical forests subjected to repeated fires. Although many ways of controlling these species have been proposed, the efficiency of different combinations of strategies and their effect on forest recovery remain unknown. Here, we assessed to what extent removing the above-ground biomass of the shade-intolerant Pteridium arachnoideum and planting trees contributed to the control of this fern and the recovery of montane Atlantic forests in south-east Brazil. In four sites dominated by P. arachnoideum we applied five treatments in adjacent plots: (1) control without interference, (2) cutting twice per year, (3) cutting twice per year plus tree planting, (4) cutting three times per year, and (5) cutting three times per year plus tree planting. Over two consecutive years we repeated the treatments and monitored P. arachnoideum height, rhizome and above-ground biomass, as well as the composition of the established vegetation and soil seed bank. We also assessed the survival and growth of the planted trees. Tree planting accounted for over half of the treatment costs, whereas increasing the cutting frequency only increased costs by 15 %. After two years, the three times per year cutting treatment caused the largest decrease in above-ground biomass (1 to 0.1 kg/m2) and abundance (80 to 15 %) of P. arachnoideum. Planted trees exhibited low survival (47 %) rates and growth (30 cm/year), and hence had no effect on the dominance of P. arachnoideum. Treatments did not affect rhizome biomass, suggesting that P. arachnoideum could regain its dominance if cutting treatments were discontinued. Although two annual cuts slightly favoured tree regeneration, trees were rare in both the established vegetation (less than 10 %) and seed bank (less than 0.3 seeds/dm3). Conversely, under three annual cuts the African grass M. minutiflora became more abundant in both the established vegetation (5 to 25 %) and seed bank (1 to 4 seeds/dm3). Our findings show that intensive mechanical removal decreases the dominance of P. arachnoideum in Atlantic forests. However, its benefits to forest recovery seem to be limited by lack of tree regeneration, impoverished seed banks and invasive grasses. Therefore, planting fast-growing and drought-tolerant tree species may be essential to the recovery of these degraded Neotropical forests.

Interactions of structural and functional characteristics of trees and bamboo in an Atlantic semideciduous forest in Southeast Brazil

Interactions of structural and functional characteristics of trees and bamboo in an Atlantic semideciduous forest in Southeast Brazil

Evapotranspiration under Drought Conditions: The Case Study of a Seasonally Dry Atlantic Forest

Information on evapotranspiration (ET) has the potential to clarify drought’s effects on the water balance of natural ecosystems. Here, we use a 6-year dataset to present daily ET trends under different drought conditions in a seasonally dry Atlantic Forest in southeast Brazil as well as environmental and biophysical controls. Generalized linear models (GLMs) were applied to highlight the main controls on ET. Significant differences for ET were not found under different drought conditions (near normal, moderately dry, and severely dry). ET responded positively to net radiation (Rn), bulk surface (gs), and aerodynamic (ga) conductance. Drought severity and soil water storage (SWS) did not significantly affect ET. We attributed the regularization of ET to (i) the stability in the SWS observed in the study site (dystrophic red latosol); (ii) the tree species’ adaptations to cope with water stress; (iii) the alternation between droughts and near-normal conditions (which have increased the water in the system on an annual basis); and (iv) the monthly variations in water inputs within the hydrological years. Finally, our study sheds light on the ecosystem characteristics that may represent sources of resilience when facing the droughts predicted in climate change scenarios.

Contributions to the knowledge of Ceroys (Miroceroys) Piza, 1936 (Phasmatodea: Heteronemiidae): two new mossy stick insects from the Atlantic Forest of Brazil.

Several lineages of stick insects (Phasmatodea) are poorly studied, especially in the Neotropics. Recently, Brazilian stick insects have been subject of significant research and, among them, the Heteronemiidae, although several genera remain to be explored. Pygirhynchini is an Heteronemiidae lineage currently containing four genera, of which three occur in Brazil: Ceroys, Canuleius and Pygirhynchus. The former is subdivided in Ceroys (Ceroys) and Ceroys (Miroceroys) based on ornamentation variations absence or presence of a spine on the scapus. We describe two new species, Ceroys (Miroceroys) cancelloae sp. nov. and Ceroys (Miroceroys) indicattii sp. nov. based on both sexes and eggs. The new species inhabit submontane, ombrophilous Atlantic Forest in Southeast Brazil and can be differentiated from other Ceroys (Miroceroys) by conspicuous features of external morphology of adults and eggs. We also briefly describe and illustrate nymphal stages of C. cancelloae sp. nov. and present notes on the biology of both species, and a distribution map for the genus, increasing the scarce knowledge of the natural history of phasmids in Brazil.

Growth ring response in Paratecoma peroba (Record) Kuhlm. of seasonal semideciduous forest in Southeast Brazil

Seasonal climate can influence the radial growth of woody species, resulting in physical indications of regional environmental events that are identifiable through dendrochronological analysis. The objective of this work was to investigate the dendroclimatological potential of the Paratecoma peroba occurring in the last remnant of seasonal semideciduous forest in Rio de Janeiro, Brazil, and to characterize the anatomical structure of its growth rings. This important species has a large ecological role in the forest as a late succession but is classified as “Endangered" because it is extensively and illegally exploited due to the use potential of wood. Samples were collected and analyzed using dendrochronological methods and wood anatomy. P. peroba has distinct annual growth rings with diffuse to semi-porous porosity, marginal parenchyma, radial flattening, and greater thickening of the fibre wall in the latewood. It may sometimes present weakly distended rays and a higher frequency of vessels in the initial wood. The analyzed individuals are 30–77 years of age and exhibit a radial increase of 2–4 mm year-1. Our results indicate that precipitation and temperature influence the growth of P. peroba in this forest and that temperature is the climatic factor with the most influence on the growth ring of the species. Given the importance of the species and the study area, knowing its growth rates and the factors that influence it is possible to offer better criteria for managing species and its reforestation for conservation.

Are recaptures of banded birds efficient at detecting altitudinal migrations in the Atlantic Forest?

Altitudinal migration in birds comprises seasonal movements between breeding and non-breeding areas in mountainous regions, attributed to biotic and abiotic factors. Different authors have suggested the existence of altitudinal migration between high and low areas of the mountains of the Atlantic Forest, with movement from high to low during the winter when birds would be fleeing the cold and in search of food, but there is no documented evidence. Through recaptures of understory birds, we investigated possible altitudinal migration in a region of the Atlantic Forest in Southeast Brazil. Twenty mist-nets were set at four locations between 15 and 729 m a.s.l. during 143 days of field work, distributed over 54 months and covering all seasons of the year. A total of 1946 birds (98 species) were captured/banded with 558 being recaptured (28.6%; 45 species). However, only 42 of the recaptures were at a different elevation. Most of the movements were of short distances and performed only once by birds, showing no seasonal pattern. These movements may be better interpreted as daily movements undertaken by birds of mixed-species flocks looking for food or moving around their respective home-ranges. Our results show that mist-nets may not be an effective tool in detecting altitudinal movements of birds and that other methods should be evaluated for this purpose.

Elevational specialization and the monitoring of the effects of climate change in insects: Beetles in a Brazilian rainforest mountain

Mountains have provided important insights on the impacts of climate change on species distribution. Organisms living in tropical mountains are expected to specialize in narrow temperature limits (demonstrating low thermal tolerance), often with narrow elevational distributions relative to temperate species, and may shift their elevational range in response to climate change. Importantly, insects are sensitive, and respond rapidly, to temperature variation, making them suitable bioindicators to monitor the effects of climate change. However, to monitor the effects of climate change in mountains it is important to understand present elevational distribution and other ecological characteristics of local insect populations. In this context, we suggest a method to identify beetle taxa that can be used to monitor climate change effects in tropical mountainous insect species. We illustrate the method by describing the elevational distribution of different beetle groups, associating this distribution with species’ thermal range in a tropical mountain forest in Southeast Brazil. Sampling was conducted at Serra dos Órgãos National Park, RJ, Brazil, in the Atlantic Rainforest, one of the main global biodiversity hotspots. In order to systematically sample beetle diversity across elevations, we used flight interception ‘Malaise’ traps at fifteen different sites, from 130 m to 2170 m a.s.l., over three consecutive months during the rainy season. To investigate species’ climatic niches, we recorded climatic variables for this period. We collected 2963 individuals of 272 species, belonging to six Coleoptera groups over a temperature gradient that decreased about 0.5 °C for each 100 m in elevation. Considering the thermal tolerance of species from tropical mountains and their narrow elevational range and abundance, five Coleoptera species belonging to Cerambycidae, Eumolpinae (Chrysomelidae), Lampyridae and Phengodidae were considered suitable bioindicators, and the Eumolpinae and Lampyridae were the ones with the narrowest elevational range. We suggest that the use of abundant species (or groups of species) with narrow elevational range as bioindicators can be valuable to monitor the effects of climate change on the biota, allowing us to evaluate how species are responding to changes over time.

More than meets the eye: syntopic and morphologically similar mangrove killifish species show different mating systems and patterns of genetic structure along the Brazilian coast.

Different mating systems can strongly affect the extent of genetic diversity and population structure among species. Given the increased effects of genetic drift on reduced population size, theory predicts that species undergoing self-fertilisation should have greater population structure than outcrossed species; however, demographic dynamics may affect this scenario. The mangrove killifish clade is composed of the two only known examples of self-fertilising species among vertebrates (Kryptolebias marmoratus and Kryptolebias hermaphroditus). A third species in this clade, Kryptolebias ocellatus, inhabits mangrove forests in southeast Brazil; however, its mating system and patterns of genetic structure have been rarely explored. Here, we examined the genetic structure and phylogeographic patterns of K. ocellatus along its distribution, using mitochondrial DNA and microsatellites to compare its patterns of genetic structure with the predominantly selfing and often-syntopic, K. hermaphroditus. Our results indicate that K. ocellatus reproduces mainly by outcrossing, with no current evidence of selfing, despite being an androdioecious species. Our results also reveal a stronger population subdivision in K. ocellatus compared to K. hermaphroditus, contrary to the theoretical predictions based on reproductive biology of the two species. Our findings indicate that, although morphologically similar, K. ocellatus and K. hermaphroditus had remarkably different evolutionary histories when colonising the same mangrove areas in southeastern Brazil, with other factors (e.g., time of colonisation, dispersal/establishment capacity) having more profound effects on the current population structuring of those species than differences in mating systems.

Storage of short-lived seeds of Inga vera subsp. affinis in osmotic medium

AbstractInga vera subsp. affinis (Fabaceae) is a tree species native to riparian forests in Southeast Brazil and is key for the restoration of deforested areas. The species produce seeds that are highly recalcitrant. Extreme sensitivity to desiccation as well as vivipary are commonly observed in mature seeds, which also tend towards polyembryony. Past research has shown that typical strategies to store seeds are inapplicable to Inga vera as viability is completely lost when seeds are either dried to around 28% water content (wet basis) or stored at 5°C for a few weeks. Here, we examine the feasibility of storing the seeds under hydrated conditions but at reduced water potential. Freshly collected seeds were kept under conventional storage conditions (plastic bags in cold chamber, 5°C) and in polyethylene glycol (PEG) solutions (−1.6 and −2.4 MPa) at 10°C. Seed germination was assessed after various intervals of time, until all seeds had lost viability. Before storage, seeds attained 100% germination and produced an average of 1.8 normal seedlings per seed (due to polyembryony). Storage in PEG at −1.6 MPa maintained 90% germination (radicle protrusion) and one normal seedling per seed on average for more than 200 d. Osmotic storage likely slowed down metabolism within the seed and hence consumption of food reserves. The storage time achieved has practical applications for in situ restoration, but cannot address ex situ germplasm conservation. Extending shelf life for an additional 6 months allows tree nurseries to optimize the production of seedlings so that they can be planted during the wet season.

Pollen morphology of Dioscorea (Dioscoreaceae) from the Atlantic Forest in southeast Brazil (São Paulo) with a contribution to the systematics of Neotropical species

Dioscorea is a highly diverse genus in Brazil, particularly the south-eastern region, and most especially in the Atlantic Forest. Recent phylogenetic studies have helped clarify the intricate subgeneric classification of the genus, although some species remain to be sampled, and further studies are necessary to ascertain their systematic placement in the genus. Here, a palynological description of nine species occurring in a patch of Atlantic Forest, in the State of São Paulo, is provided. Three of these have not yet been sampled in molecular studies. The pollen of D. tauriglossum had never been characterised before. Dioscorea pollen are spheroidal to ellipsoidal, small to medium in size (the larger dimensions in D. dodecaneura and the smaller in D. amaranthoides), with disulculate aperture. The pollen wall ornamentation contributed to the segregation of two species – psilate-perforate (D. altissima, syn. D. choncrocarpa) and rugulate-perforate (D. monadelpha), as well as the separation of the other seven species in two groups – microreticulate (D. dodecaneura, D. multiflora, D. tauriglossum), and striate (D. amaranthoides, D. laxiflora, D. olfersiana, D. trilinguis). Systematic implications of the palynological variation found are discussed under the light of recent molecular phylogenetic studies.

Seasonal and habitat structure of an anuran assemblage in a semideciduous forest area in Southeast Brazil

In this study, we evaluated the reproductive activity and the temporal and spatial distributions of anuran assemblages in three environments within a semideciduous forest in Southeast Brazil, located at Municipality of Barão de Monte Alto, State of Minas Gerais, Brazil. The field activities were carried out during three consecutive days, monthly throughout the rainy seasons of 2013-2014 and 2014-2015. We recorded 28 anurans species, distributed in eight families. We observed the spatial-temporal distribution of some species, and their associated reproductive behaviors through exploration of vocalizations at different sites. The spatial and temporal distribution of the species seems to adapt to abiotic and biotic factors of their environment.

Surviving in the shadows: Light responses of co-occurring Rubiaceae species within a tropical forest understory

The question of how closely related species coexist in the heavily shadowed understory of tropical forests is still not properly clarified. We studied the magnitude of trait variation in twelve morphological, nutritional, and physiological traits related to light response in 85 individuals belonging to nine co-occurring Rubiaceae species in the understory of a tropical forest in Southeast Brazil. Three topographic habitats were chosen from shadowed and more fertile soils in the valley to more open canopies and less fertile soils at slopes and ridge, which were characterized using soil and canopy cover variables. Light curves (A/PAR) were adjusted in five leaves (one leaf per individual plant) for each species and habitat. We compared soil and canopy variables among the topographic habitats, quantified trait dispersion among the nine species, and tested for differences in trait values among habitats and species. Ridge habitat showed the lowest resource availabilities, soil nutrients and water, and a more open canopy. Species distributions in the gradient were best explained by specific leaf area (SLA) and leaf thickness. The Rubiaceae species showed a great investment in leaf nitrogen, regardless of habitat or species. Ridge species maintained low dark respiration rates and light compensation points. Higher SLA values for valley plants allowed greater light-saturated net photosynthetic rates on a mass basis, providing higher positive net carbon balance when compared to the plants in the slope and ridge. Living and survival of these shade plants are possible by very low mean light compensation points. We show that trait differences among topographic habitats in coexisting species of Rubiaceae are important to understanding the organization of tropical forests understory “species swarms”.

First record of Pterygodermatites (Pterygodermatites) (Nematoda: Rictulariidae) in South America, with the description of a new species from the Atlantic Forest, southeast Brazil.

Pterygodermatites are parasitic nematodes found in mammals worldwide, except Australia. There are 38 described species, which are found parasitizing bats, rodents, marsupials, carnivores and primates. We present taxonomic notes on nematodes collected from the digestive tract of bats from the Atlantic Forest in southeast Brazil. Two species previously recorded in marsupials in this region, Pterygodermatites (Paucipectines) elegans and Pterygodermatites (Paucipectines) jagerskioldi, were found parasitizing molossid and vespertilionid bats, respectively. A new species Pterygodermatites (Pterygodermatites) atlanticaensis found in phyllostomid bats is here described. Despite earlier work based on females, taxonomic work on this group of nematodes is usually based on male characters, such as position and number of caudal papillae, which is a problem because males are short-lived and not easily found. Here we also describe in detail female characters, such as position of the last pair of cuticular processes relative to the posterior end. This is the first record of P. (Pterygodermatites) in South America and the first record of P. (Paucipectines) jagerskioldi in bats.

Runoff, soil loss, and sources of particulate organic carbon delivered to streams by sugarcane and riparian areas: An isotopic approach

Soil erosion leads to land degradation and translocation of soil particles together with associated particulate organic carbon (POC) and nutrients, thereby influencing the global carbon cycle. In the present study, we estimated the contribution of POC delivered to a first-order stream from upslope sugarcane fields and a riparian forest in southeast Brazil. The results show that the amount of surface runoff and soil erosion generated in the riparian forest is significantly lower than in the upslope sugarcane field. However, the contribution of the forest to the total stream bed POC was above 70%, even though most sediments delivered to the stream originated from the upland sugarcane fields. The discrepancy between sediment and POC delivery from both land uses is a consequence of the presence of preferential runoff pathways from the agricultural fields, through the buffer strips, to the stream. This disconnection between the main sources of sediment and POC to the first-order stream is a potentially important mechanism influencing the transfer of POC from upslope areas to waterways. This mechanism should be considered in order to more reliably assess fluxes of OC from upslope areas to first-order streams in landscapes where arable land is separated from streams by a semi-natural buffer zone with permanent vegetation.

New Species ofOxycheilaDejean (Coleoptera: Carabidae: Megacephalini) from the Atlantic Forest of Southeast Brazil

Oxycheila thanatus sp. nov. (Coleoptera, Carabidae) is described, based on specimens collected near a waterfall in the National Park of Caparao, a Conservation Unit of Atlantic Forest in Southeast Brazil. This new species is most similar to Oxycheila germaini Fleutiaux, 1893, but has a triangular labrum which is wider than long, the elytral punctation is subseriate and the humeral calli is comparatively less developed. A modified couplet of the available taxonomic key to Oxycheila species is provided to include O. thanatus.

Pollination biology of melittophilous legume tree species in the Atlantic Forest in Southeast Brazil

This study describes the floral phenology and morphology, reproductive biology and pollinators for eight legume tree species, Schizolobium parahyba, Senna macranthera, and Senna multijuga (Caesalpinioideae), as well as Andira fraxinifolia, Lonchocarpus cultratus, Pterocarpus violaceus, Swartzia oblata, and S. simplex (Papilionoideae), in the Atlantic Forest in Southeast Brazil. All the studied species showed an annual flowering pattern, and almost all are of the cornucopia-flowering type, with the only exception being Swartzia oblata, which was of the steady-state type. In general, the legume flowers studied are conspicuous, mostly medium-sized, and offer nectar and/or pollen as a resource. Self-incompatibility associated with the production of many flowers and consequent pollen discounting due to self-pollination may contribute to low fruit set of these species in natural conditions. Fifty bee species were recorded visiting the flowers, with medium to large-sized Apidae bees, such as Bombus morio, and species of Xylocopa, Centridini and Euglossina, which were among the most frequent visitors and major pollinators. These bees showed high floral constancy, thus they are significant to the reproductive success of these tree species. This study provides information regarding the interactions between bees and these eight legume species and evaluates the importance of pollinators for their sexual reproduction.

Pollination biology of melittophilous legume tree species in the Atlantic Forest in Southeast Brazil

ABSTRACT This study describes the floral phenology and morphology, reproductive biology and pollinators for eight legume tree species, Schizolobium parahyba, Senna macranthera, and Senna multijuga (Caesalpinioideae), as well as Andira fraxinifolia, Lonchocarpus cultratus, Pterocarpus violaceus, Swartzia oblata, and S. simplex (Papilionoideae), in the Atlantic Forest in Southeast Brazil. All the studied species showed an annual flowering pattern, and almost all are of the cornucopia-flowering type, with the only exception being Swartzia oblata, which was of the steady-state type. In general, the legume flowers studied are conspicuous, mostly medium-sized, and offer nectar and/or pollen as a resource. Self-incompatibility associated with the production of many flowers and consequent pollen discounting due to self-pollination may contribute to low fruit set of these species in natural conditions. Fifty bee species were recorded visiting the flowers, with medium to large-sized Apidae bees, such as Bombus morio, and species of Xylocopa, Centridini and Euglossina, which were among the most frequent visitors and major pollinators. These bees showed high floral constancy, thus they are significant to the reproductive success of these tree species. This study provides information regarding the interactions between bees and these eight legume species and evaluates the importance of pollinators for their sexual reproduction.

Ecological outcomes and livelihood benefits of community‐managed agroforests and second growth forests in Southeast Brazil

AbstractThe Forest and Landscape Restoration movement has emerged as an approach to reconcile biodiversity conservation, ecosystem services provisioning and human well‐being in degraded landscapes, but little is known so far about the potential of different reforestation methods to achieve these objectives. Based on this gap, we assessed the ecological outcomes and local livelihood benefits of community‐managed agroforests and second growth forests to assist natural regeneration in the coastal Atlantic Forest of Brazil. We investigated and compared agroforests and secondary forests according to their structure and floristic composition in 51 circular plots of 314 m², their role in supporting local livelihoods (45 semi‐structured interviews) and the use and cultural importance of plant species (61 interviews). Agroforests and, more remarkably, managed secondary forests (1) re‐established a well‐developed forest structure, with a higher density of tree‐sized individuals and similar basal area compared to nearby old growth forests; (2) were composed by a rich array of native species, including five threatened species, but had lower species richness than old growth remnants; and (3) improved local livelihoods by supplying market valuable and culturally important plants, including 231 native ethnospecies. Overall, local production systems showed remarkable potential to engage smallholders of developing tropical countries in Forest and Landscape Restoration and contribute to achieve its overall goals. We advocate the promotion of these systems as effective Forest and Landscape Restoration approaches in multi‐scale programs and policies.

Pollution emissions from a petrochemical complex and other environmental stressors induce structural and ultrastructural damage in leaves of a biosensor tree species from the Atlantic Rain Forest

Tibouchina pulchra (Cham.) Cogn., a pioneer tree species commonly found in the Atlantic Rain Forest in southeast Brazil, proved to be a promising biosensor species for indicating the potential effects of air pollution on this type of forest. This study aimed to investigate whether the present levels of nitrogen oxides, sulfur oxides, ozone and particulate matter in industrial and urban areas in the Cubatão region induce structural and ultrastructural changes in this species, to explore the synergy between air pollutants and the effects of other environmental stressors as contributors to microscopic symptoms and to verify whether these changes are seasonally dependent. The field study consisted of exposing potted saplings of T. pulchra in three sites around the petrochemical industry (industrial area), in downtown Cubatão (urban area) and in a site far from the industrial emissions (reference area). An experimental study using open top chambers (OTC) with filtered air and non-filtered air was also carried out to confirm the field results. Four exposure periods of 12 weeks were conducted during the field and the OTC experiments, covering wet and dry periods. The effects were more pronounced in plants exposed in the industrial areas during the dry period, which were confirmed by OTC experiments. Crystal density variations were linearly explained by decreases in NO2, SO2 and the sum of all hourly concentrations of ozone (SUM00), as well as increases in PM10, relative humidity and rainfall. Variations in lipid peroxidation correlated positively with air temperature and SUM00. Tannins and starch grains decreased. Collapsed and sinuous cell walls, dense cytoplasm, invagination of plasma membranes and vacuome were the main effects observed at the structural level. Occurrence of pectic warts, alterations in chloroplasts and numerous vesicles close to the cell walls were the main ultrastructural effects detected. These results confirmed that pollutants and environmental variables acted synergistically to induce oxidative stress in this species, enhancing our understanding of the bioindicator potential of T. pulchra for monitoring complex mixtures of air pollutants in the tropics.