Forests Of Costa Rica Research Articles

Increasing Ages of Inga punctata Tree Soils Facilitate Greater Fungal Community Abundance and Successional Development, and Efficiency of Microbial Organic Carbon Utilization

Leguminous Inga trees are thought to enhance soil carbon (C) accumulation following reforestation, through mostly unknown mechanisms. This study amplified soil DNA using the ITS1F and ITS4 primers for PCR and Illumina MiSeq methods to identify fungal taxa, and traditional C analysis methods to evaluate how planted 4-, 8-, and 11-year-old Inga punctata trees affected soil fungal community compositions and C utilization patterns compared to old-growth I. punctata trees and an adjacent unplanted pasture within the same reforestation zone in Monteverde, Costa Rica. Along the tree age gradient, the planted I. punctata trees enhanced the tree soil C capture capacity, as indicated by increased levels of soil biomass C, Respiration, and efficiency of organic C use (with lower qCO2 values), and development of increasingly more abundant, stable, and successionally developed fungal communities, including those associated with the decomposition of complex organic C compounds. The level and strength of differences coincided with differences in the time of separation between the pasture and tree age or between the different tree ages. Fungal taxa were also identified as potential indicators of the early and late stages of soil recovery. Thus, planting I. punctata should be part of future reforestation strategies used in this region of the Monteverde Cloud Forest in Costa Rica.

Significance of AI-assisted techniques for epiphyte plant monitoring and identification from drone images

Monitoring forest canopies is vital for ecological studies, particularly for assessing epiphytes in rain forest ecosystems. Traditional methods for studying epiphytes, such as climbing trees and building observation structures, are labor, cost intensive and risky. Unmanned Aerial Vehicles (UAVs) have emerged as a valuable tool in this domain, offering botanists a safer and more cost-effective means to collect data. This study leverages AI-assisted techniques to enhance the identification and mapping of epiphytes using UAV imagery. The primary objective of this research is to evaluate the effectiveness of AI-assisted methods compared to traditional approaches in segmenting/identifying epiphytes from UAV images collected in a reserve forest in Costa Rica. Specifically, the study investigates whether Deep Learning (DL) models can accurately identify epiphytes during complex backgrounds, even with a limited dataset of varying image quality. Systematically, this study compares three traditional image segmentation methods Auto Cluster, Watershed, and Level Set with two DL-based segmentation networks: the UNet and the Vision Transformer-based TransUNet. Results obtained from this study indicate that traditional methods struggle with the complexity of vegetation backgrounds and variability in target characteristics. Epiphyte identification results were quantitatively evaluated using the Jaccard score. Among traditional methods, Watershed scored 0.10, Auto Cluster 0.13, and Level Set failed to identify the target. In contrast, AI-assisted models performed better, with UNet scoring 0.60 and TransUNet 0.65. These results highlight the potential of DL approaches to improve the accuracy and efficiency of epiphyte identification and mapping, advancing ecological research and conservation.

Nonbreeding distributions of four declining Nearctic–Neotropical migrants are predicted to contract under future climate and socioeconomic scenarios

ABSTRACT Climate and land use/land cover change are expected to influence the stationary nonbreeding distributions of 4 Nearctic–Neotropical migrant bird species experiencing population declines: Cardellina canadensis (Canada Warbler), Setophaga cerulea (Cerulean Warbler), Vermivora chrysoptera (Golden-winged Warbler), and Hylocichla mustelina (Wood Thrush). Understanding how and where these species’ distributions shift in response to environmental drivers is critical to inform conservation planning in the Neotropics. For each species, we quantified current (2012 to 2021) and projected future (2050) suitable climatic and land use/land cover conditions as components of stationary nonbreeding distributions. Multi-source occurrence data were used in an ensemble modeling approach with covariates from 3 global coupled climate models (CMCC-ESM2, FIO-ESM-2-0, MIROC-ES2L) and 2 shared socioeconomic pathways (SSP2-RCP4.5, SSP5-RCP8.5) to predict distributions in response to varying climatic and land use/land cover conditions. Our findings suggest that distribution contraction, upslope elevational shifts in suitable conditions, and limited shifts in latitude and longitude will occur in 3 of 4 species. Cardellina canadensis and S. cerulea are expected to experience a moderate distribution contraction (7% to 29% and 19% to 43%, respectively), primarily in response to expected temperature changes. The V. chrysoptera distribution was modeled by sex, and females and males were projected to experience a major distribution contraction (56% to 79% loss in suitable conditions for females, 46% to 65% for males), accompanied by shifts in peak densities to higher elevations with minimal changes in the upper elevation limit. Expected changes in precipitation had the greatest effect on V. chrysoptera. Hylocichla mustelina experienced the smallest distribution change, consistent with the species’ flexibility in habitat selection and broader elevational range. We recommend defining priority areas for conservation as those where suitable conditions are expected to remain or arise in the next 25 years. For V. chrysoptera in particular, it is urgent to ensure that mid-elevation forests in Costa Rica and Honduras are adequately managed and protected.

Light drives nitrogen fixation in tropical montane cloud forests in Costa Rica

Tropical montane cloud forests are high altitude ecosystems characterized by very high ambient humidity, which favors organisms that depend on the environment for their water status, such as bryophytes and their nitrogen-fixing symbionts. Bryophyte-associated N2 fixation is a major source of new N in several northern environments, but their contributions to the N cycle in other ecosystems is still poorly understood. In this work, we evaluated N2 fixation rates associated with epiphytic bryophytes growing along the stems of pumpwood trees (Cecropia sp.) as well as in surrounding litter and soil from a primary and a secondary cloud forests in the Talamanca Mountain Range, Costa Rica. Nitrogen fixation was significantly higher in substrates from the secondary forest compared to those from the primary forest. Overall, N2 fixation rates associated with epiphytic bryophytes were 57 times those of litter and 270 times what was measured in soil. Further, light intensity was the major factor influencing N2 fixation rates in all substrates. Increased access to light in disturbed cloud forests may therefore favor bryophyte-associated N2 fixation, potentially contributing to the recovery of these ecosystems.

Clusia salicifolia (Clusiaceae), a new hemiepiphyte species from Costa Rica and Panama

SummaryWe describe and illustrate Clusia salicifolia (Clusiaceae) a new species from Central America. This new species is different to all related species for its hemiepiphytic habit, narrow leaves with acute to acuminate apex, reticulate resin canals, staminate flowers with quadrangular androecium with 30 – 47 free stamens, and pistillate flowers with 4 stigmas and 4 pairs of stamen-like staminodia. The species is known from a limited number of localities in lowland wet forests in Costa Rica and Panama and is here assessed as endangered under the IUCN criteria.

Edge effects and social behavior in three platyrrhines.

Social behavior is a key adaptation for group-living primates. It is important to assess changes to social behavior in human-impacted landscape zones to better understand the impact of anthropogenic disturbance on primate species. We investigated social behavior rate and type in three species of platyrrhines across 100 m anthropogenic edge and interior zones of a fragmented forest in Costa Rica, La Suerte Biological Research Station (LSBRS). Following results from other sites, we predicted that spider monkeys (Ateles geoffroyi), capuchin monkeys (Cebus imitator) and howler monkeys (Alouatta palliata) would show lower rates and fewer types of social behavior in forest edge compared to interior. We collected 1341 h of instantaneous focal data from 2017 to 2023 across the three monkey species. We found mixed support for our predictions, with spider and capuchin monkeys modifying some but not all aspects of social behavior across forest zones at LSBRS. Spider monkeys had lower rates of social behavior and capuchin monkeys performed different types of social behaviors in forest edge compared to interior at LSBRS. In contrast, howler monkeys did not modify social behavior. Two out of three platyrrhine species altered their social behavior when in anthropogenic edges, indicating behavioral adjustment when in human-altered habitat areas at LSBRS.

Tropical dry forest response to nutrient fertilization: a model validation and sensitivity analysis

Abstract. Soil nutrients, especially nitrogen (N) and phosphorus (P), regulate plant growth and hence influence carbon fluxes between the land surface and atmosphere. However, how forests adjust biomass partitioning to leaves, wood, and fine roots in response to N and/or P fertilization remains puzzling. Recent work in tropical forests suggests that trees increase fine root production under P fertilization, but it is unclear whether mechanistic models can reproduce this dynamic. In order to better understand mechanisms governing nutrient effects on plant allocation and improve models, we used the nutrient-enabled ED2 model to simulate a fertilization experiment being conducted in a secondary tropical dry forest in Costa Rica. We evaluated how different allocation parameterizations affected model performance. These parameterizations prescribed a linear relationship between relative allocation to fine roots and soil P concentrations. The slope of the linear relationship was allowed to be positive, negative, or zero. Some parameterizations realistically simulated leaf, wood, and fine root production, and these parameterizations all assumed a positive relationship between relative allocation to fine roots and soil P concentration. Model simulations of a 30-year timeframe indicated strong sensitivity to parameterization and fertilization treatment. Without P fertilization, the simulated aboveground biomass (AGB) accumulation was insensitive to the parameterization. With P fertilization, the model was highly sensitive to the parameterization and the greatest AGB accumulation occurred when relative allocation to fine roots was independent of soil P. Our study demonstrates the need for simultaneous measurements of leaf, wood, and fine root production in nutrient fertilization experiments and for longer-term experiments. Models that do not accurately represent allocation to fine roots may be highly biased in their simulations of AGB, especially on multi-decadal timescales.

A new species of planthopper in the genus Anotia (Hemiptera: Auchenorrhyncha: Derbidae) from the Los Angeles cloud forest in Costa Rica.

Recent survey work in Costa Rica has resulted in the discovery of new species of derbid and cixiid planthoppers associated with palms. During this survey, one species belonging to the genus Anotia has been discovered and described, A. firebugia. A second species was collected while sweeping trailside vegetation in the Los Angeles cloud forest in Costa Rica and determined to represent a new species of Anotia. Herein, Anotia cerebro sp. n. is described and supplemental molecular data for the cytochrome c oxidase subunit I (COI), 18S rRNA gene and D9-D10 expansion region of the 28S rRNA gene are provided.

Effects of large mammal exclusion on seedling communities depend on plant species traits and landscape protection in human‐modified Costa Rican forests

Abstract Large terrestrial herbivorous mammals (LTH‐mammals) influence plant community structure by affecting seedling establishment in mature tropical forests. Many of these LTH‐mammals frequent secondary forests, but their effects on seedling establishment in them are understudied, hindering our understanding of how LTH‐mammals influence forest regeneration in human‐modified landscapes. We tested the hypothesis that the strength of LTH‐mammals' effects on seedling establishment depends on landscape protection, forest successional stage and plant species' traits using a manipulative field experiment in six 1‐ha sites with varying successional age and landscape protection. In each site, we established 40 seedling plot‐pairs, with one plot excluding LTH‐mammals and one not, and monitored seedlings of 116 woody species for 26 months. We found significant effects of LTH‐mammal exclusion on seedling survival contingent upon the protection of forests at the landscape level and forest stage. After 26 months, survival differences between LTH‐mammal exclusion and non‐exclusion treatments were greater in protected than unprotected landscapes. Additionally, plant species' traits were related to the LTH‐mammals' differential effects, as LTH‐mammals reduced the survival of seedlings of larger‐seeded species the most. Overall, LTH‐mammals' effects translated into significant shifts in community composition as seedling communities inside and outside the exclosures diverged. Moreover, lower density and higher species diversity were found as early as 12 and 18 months outside than inside exclosures. Synthesis and applications. Insight into the interactions between LTH‐mammals and seedling communities in forest regeneration can be instrumental in planning effective restoration efforts. We highlight the importance of landscape protection in seedling survival and the role of LTH‐mammals in promoting seedling diversity in mature forests but also in secondary successional forests. The findings suggest that conservation efforts and possibly trophic rewilding can be important approaches for preserving diversity and influencing the trajectory of secondary tropical forest succession. However, we also caution that an overabundance of LTH‐mammals may adversely impact the pace of forest succession due to their preference for large‐seeded species. Therefore, a comprehensive wildlife management plan is indispensable. Additionally, longer term studies on LTH‐mammals are necessary to understand the effects of temporal fluctuations that are undetected in short‐term studies.

Bacterial multi-resistance to antibiotics in water from plant cavities (phytotelms) in a deep tropical forest from Costa Rica

Introduction: The spread of antimicrobial resistance in natural environments continues to be reported throughout the world; nevertheless, there is no study about phytotelms (water in the natural cavities of plants) in the deep understory of tropical cloud forests from Costa Rica. Objective: To detect phytotelm antibiotic-resistant bacterial strains in a Costa Rican forest and nearby town. Methods: We used the disk diffusion method to analyze the antimicrobial resistance of 10 gram-negative bacterial strains from phytotelm water collected from 10 bromeliads and 10 heliconias in both sites. Results: Three strains were multidrug resistant to more than three antibiotics in each site, and only one strain was susceptible to all antibiotics. Antibiotic resistance was similar in both environments. Conclusions: Phytotelm can be a rapid, cost-effective, and simple source for detecting antimicrobial resistance in unexplored environments.

Climate, as well as branch-level processes, drive canopy soil abundance and chemistry

Canopy soils can be important to forest nutrient cycling, hydrology, and biodiversity, but the factors determining their distribution and properties are largely unknown. We surveyed canopy soils across gradients of temperature and precipitation in six primary forests in Costa Rica. We used solid-state 13C nuclear magnetic resonance (NMR) and mass spectrometry (MS) to understand how the composition of canopy soil organic matter varies within and across sites. Climate, particularly fog, appears to drive canopy soil abundance across forests, while tree size determines canopy soil abundance within a forest. Canopy soil chemistry mostly varied within sites, though temperature was associated with the carbon (C) to nitrogen ratio, total dissolved nitrogen, and alkyl-C abundance, while fog explained some of the variation in dissolved organic carbon and O-alkyl C abundance. This study is the first-ever glimpse into large- and small-scale drivers of canopy soil abundance and biochemical composition. Our results highlight the importance of tree size and fog in determining the quantity and quality of canopy soil organic matter, suggesting that canopy soil stocks may be particularly vulnerable to climate and land use change. Identifying how multi-scale factors influence canopy organic matter processes will enhance our ability to identify and predict how environmental change might affect the abundance and chemistry of canopy soils and thus biodiversity that they support.

Let it Grow (Back): A Call for the Conservation of Secondary Forests as Medicinal Plant Habitat

Costa Rica is widely regarded as a global leader in conservation practices. In the Maquenque National Wildlife Refuge (MNWLR), within Costa Rica’s Northern Zone, a strong commitment to conservation has led to protecting highly biodiverse mature forests. However, a significant opportunity to strengthen conservation in this region is being overlooked at a great cost to the local community and environment: the protection of regenerating secondary forests. Secondary forests account for over 50% of global tropical forests and serve vital ecological and cultural functions. Within the MNWLR, many species in the secondary forests provide medicinal value to the rural communities where western medical care is difficult to access. Recent research, however, has shown that secondary forests in Costa Rica are re-cleared within 20 years, before they have accumulated the previously lost biomass and biodiversity. In this paper, we call for conservation and management strategies to incorporate community held knowledge about culturally significant species, and for there to be economic incentives for keeping secondary forests intact and for determining which forests are designated as Protected Areas. We discuss previous research with two trees that are common in secondary forests in the MNWLR (Vismia macrophylla and Pentaclethra macroloba), recognizing that these are some of the many species that have great potential to both the ecological and social communities. While our focus area is in the Northern Zone of Costa Rica, the integration of community use and local knowledge into conservation should be a global priority.

Using stable isotopes to measure the dietary responses of Costa Rican forest birds to agricultural countryside

How human modification of native habitats changes the feeding patterns and nutritional ecology of tropical birds is critical to conserving avian biodiversity, but tropical bird diets are laborious to investigate using the traditional methods of diet analysis. Stable isotope analysis provides a cost-effective and efficient proxy to identify general foraging patterns, especially when dietary shifts spanning multiple trophic levels have occurred due to ecosystem disturbance or transformation. To characterize the diets of forest bird species that persist in tropical agricultural countryside, we compared feather carbon (δ13C) and nitrogen (δ15N) isotope values of four species caught and radio-tracked in a 270 hectare forest reserve, smaller forest remnants (including mature forest, secondary forest, and riparian strips), and coffee plantations in mid-elevation (ca. 800–1,400 m) southern Costa Rica. Bird habitat choice had a significant effect on diet composition as revealed by δ13C and δ15N values. Three of the four species studied showed evidence of significantly reduced consumption of invertebrates in coffee plantations, with the isotope values of two species (Tangara icterocephala and Mionectes oleaginous) indicating, by comparison, nearly a doubling of invertebrate consumption in forest remnants. Our results suggest that coffee plantations are deficient in invertebrates preferred by forest generalist birds that forage in both native forest remnants and coffee plantations. In this region, typical of mountainous American tropics, small forest remnants and a larger forest reserve provide critical dietary resources for native forest birds that utilize the agricultural countryside.

A new species of planthopper from Costa Rica in the genus Herpis (Hemiptera: Derbidae) from palms in the cloud forest

Recent survey efforts in Costa Rica have documented many new species of planthoppers, primarily in the families Derbidae and Cixiidae, on palms. Recently, a specimen was collected sweeping palms in the Los Angeles cloud forest in Costa Rica and was identified as belonging to the genus Herpis (Derbidae). It was subsequently determined to represent a previously undescribed species. Herein, the new species, Herpis circumsoros Bahder & Bartlett sp. n. is described with supplemental molecular data for the cytochrome c oxidase subunit I (COI) gene and 18S rRNA gene to support placement of the new species in the genus Herpis.

Early successional habitat supports unique avian communities dominated by wintering migrants in a premontane tropical forest

Abstract Forest succession drives concomitant changes in associated faunal communities. Thus, maintaining landscapes with high successional diversity can be an important consideration in habitat management. We sought to describe avian community characteristics across a successional gradient created by reforestation efforts in a tropical premontane wet forest in Costa Rica. Specifically, we examined the effects of successional stage on overall abundance, species richness, diet niche, migratory status, and community composition. We hypothesised that these metrics of bird abundance, diversity and community composition would differ across successional stages. Using data from transects conducted in 2018, we found that several metrics of avian abundance, diversity and community composition varied as a function of successional stage. Surprisingly, the earliest successional stage exhibited the greatest abundance, species richness and proportion of migrant species. We suggest that an ephemeral vegetation structure present for only a short period (early in succession) creates a unique habitat that results in a distinct avian community. This highlights the potential importance of early successional forests for avian communities, especially neotropical migrants.

Underestimated Neotropical diversity: Integrative taxonomy reveals two unrelated look-alike species in a suboscine bird (Pachyramphus albogriseus)

Abstract We applied an integrative taxonomic framework to evaluate the systematics of the Neotropical Black-and-white Becard (Pachyramphus albogriseusSclater 1857). Combining phylogenomic (ultraconserved elements), morphological, and vocalization data, we confirmed that this species is polyphyletic; some individuals form a clade sister to P. polychopterus and should be afforded species rank as P. salviniRichmond 1899 (Slender-billed Becard), whereas the remaining subspecies of P. albogriseus (Broad-banded Becard) are sister to P. major. We found that P. salvini differs from P. albogriseus in song, color of the lores, wing-bar width, body size, and bill width. Whereas P. albogriseus occurs in montane forest in Costa Rica and Panama (ssp. ornatus) and along the eastern slope of the Andes from northern Venezuela to southern Peru (ssp. albogriseus), P. salvini is found in the lowlands from Pacific Colombia south to northwest Peru and in the Río Marañón drainage. The latter also occurs, possibly only seasonally, along the eastern slope of the Andes, where the two species’ ranges approach closely. We treat P. a. guayaquilensisZimmer 1936 as a junior synonym of P. salviniRichmond 1899, and P. a. coronatusPhelps and Phelps 1953 as a junior synonym of P. a. albogriseusSclater 1857. This study provides a striking example of a major problem for comparative biology: underestimated and mischaracterized diversity. We argue that there are likely many more cases like this awaiting discovery.

Using an on-site laboratory for fecal steroid analysis in wild white-faced capuchins

Hormone laboratories located “on-site” where field studies are being conducted have a number of advantages. On-site laboratories allow hormone analyses to proceed in near-real-time, minimize logistics of sample permits/shipping, contribute to in-country capacity-building, and (our focus here) facilitate cross-site collaboration through shared methods and a shared laboratory. Here we provide proof-of-concept that an on-site hormone laboratory (the Taboga Field Laboratory, located in the Taboga Forest Reserve, Costa Rica) can successfully run endocrine analyses in a remote location. Using fecal samples from wild white-faced capuchins (Cebus imitator) from three Costa Rican forests, we validate the extraction and analysis of four steroid hormones (glucocorticoids, testosterone, estradiol, progesterone) across six assays (DetectX® and ISWE, all from Arbor Assays). Additionally, as the first collaboration across three long-term, wild capuchin field sites (Lomas Barbudal, Santa Rosa, Taboga) involving local Costa Rican collaborators, this laboratory can serve as a future hub for collaborative exchange.

Aeroscapes and the Sensory Ecology of Olfaction in a Tropical Dry Forest

Aeroscapes—dynamic patterns of air speed and direction—form a critical component of landscape ecology by shaping numerous animal behaviors, including movement, foraging, and social and/or reproductive interactions. Aeroecology is particularly critical for sensory ecology: air is the medium through which many sensory signals and cues propagate, inherently linking sensory perception to variables such as air speed and turbulence. Yet, aeroscapes are seldom explicitly considered in studies of sensory ecology and evolution. A key first step towards this goal is to describe the aeroscapes of habitats. Here, we quantify the variation in air movement in two successional stages (early and late) of a tropical dry forest in Costa Rica. We recorded air speeds every 10 seconds at five different heights simultaneously. Average air speeds and turbulence increased with height above the ground, generally peaked midday, and were higher overall at the early successional forest site. These patterns of lower air speed and turbulence at ground level and overnight have important implications for olfactory foraging niches, as chemotaxis is most reliable when air movement is low and steady. We discuss our results in the context of possible selective pressures and observed variation in the foraging ecology, behaviors, and associated morphologies of resident vertebrates, with a focus on mammals. However, these data also have relevance to researchers studying socioecology, invertebrate biology, plant evolution, community ecology and more. Further investigation into how animals use different forest types, canopy heights and partition activities across different times of day will further inform our understanding of how landscape and sensory ecology are interrelated. Finally, we emphasize the timeliness of monitoring aeroecology as global wind patterns shift with climate change and human disturbance alters forest structure, which may have important downstream consequences for biological conservation.

Two Co-occurring Liana Species Strongly Differ in Their Hydraulic Traits in a Water-Limited Neotropical Forest

Lianas are a key growth form in tropical forests. They are believed to be strong competitors for water, thanks to their presumed efficient vascular systems. However, despite being a large polyphyletic group, they are currently often considered as a functionally homogeneous entity. In this study, we challenged this assumption by estimating the variability in hydraulic traits of two common, co-occurring liana species in a water-limited environment, namely, a seasonally dry tropical forest in Costa Rica. We measured vulnerability to embolism at the leaf and branch levels using two different methods (optical and acoustic vulnerability) and found that both species had very different hydraulic properties. Compared to reported P50values in literature, we found two extreme P50values: a low value forBignonia diversifolia(−4.30 ± 0.54 MPa at the leaf level; −7.42 ± 0.54 MPa at the branch level) and a high value forCissus microcarpa(−1.07 ± 0.14 at the leaf level; −1.20 ± 0.05 MPa at the branch level). Furthermore,B. diversifoliahad a higher apparent modulus of elasticity in the radial direction (556.6 ± 401.0 MPa) and a variable midday water potential. On the other hand,C. microcarpahad a low apparent modulus of elasticity in the radial direction (37.8 ± 26.3 MPa) and a high branch water content, which enabled the species to keep its water potential stable during the dehydration experiments and during a drought period in the field. This mechanism may enable this species to coexist with species that are more resistant to drought-induced embolisms such asB. diversifolia. Although only two species were studied, considerable overlap was found between the range of hydraulic properties of trees growing in the same location and trees and lianas growing in two forests in Panama. These findings demonstrate that lianas cannot be considered as a homogeneous group and call for further research into the intra-growth form diversity of liana properties.

Phoretic specialization on insect herbivores facilitates mite transportation to host plants

AbstractPhoresy, the use of another organism for dispersal, is one of the most intriguing commensalistic interactions. The selection of a correct host is fundamental for phoretic organisms to ensure arrival to suitable habitats and to encounter potential mates. This study focuses on a group of phoretic mites in the genus Lasioseius (Acari: Blattisociidae). In La Selva Biological Station, a tropical wet forest in Costa Rica, Lasioseius mites feed on nematodes inside the scrolls formed by the young leaves of their hosts, plants in the order Zingiberales. When leaves expand and unfurl, mites disperse to another rolled leaf by clinging onto ‘rolled‐leaf beetles’, a group of insect herbivores specialized on Zingiberales (genera Cephaloleia and Chelobasis; Coleoptera: Chrysomelidae). In this study, we determined whether Lasioseius mites associated with Cephaloleia belti Baly are specialized on this beetle species as a phoretic host. Mites may also be attracted to Chelobasis perplexa Baly, sharing the same host plant. Another possibility is that Lasioseius mites are opportunistic generalists and attach to rolled‐leaf beetle species (e.g., Cephaloleia dorsalis Baly) that never share host plants with either C. belti or Ch. perplexa. In a laboratory setting, we tested whether mites preferred scents from and/or attached to particular beetle species. Scent attraction experiments showed that Lasioseius mites collected from C. belti were attracted to scents from C. belti and Ch. perplexa, but were not attracted to scents from C. dorsalis. Mites collected from C. belti attached to both C. belti and Ch. perplexa, but never to C. dorsalis. In conclusion, Lasioseius mites are not strict specialists, but are able to detect and attach to beetle species that inhabit their host plant.