Forensic Science Research Research Articles

Forensic Science Research on the Web of Science Database Over 22 Years: A Bibliometric Analysis

Background: We reviewed the literature on forensic genetics from 1991 to 2022, emphasizing short tandem repeats. The objectives were to assess the caliber of research and draw attention to issues facing scientists and practitioners.Methods: Except for China, a metrological study was performed on forensic literature from 1991 to 2022 using the Web of Science (WoS).Results: The “journal of forensic sciences” had 380 papers that were the most read out of 2139 publications in the study. Also, “forensic science international: Genetics” had the most citations. Analysis with R software showed that 2001 was the peak publication year. “Journal of forensic sciences” and Budowle B were the most prolific. “Forensic science international: genetics” and Heller MJ received the most citations, and “forensic science”, “STRs”, and “DNA typing” were among the frequently used keywords.Conclusion: The study could help scientists understand the trends of short tandem repeats based on forensic science research by highlighting the top ten research institutions, funding prospects, authors, and high-citation journals to anticipate future research directions.

Morphometric analysis of the skull base and palatal regions for gender identification using CBCT: a retrospective study.

The objectives of this study were to evaluate the accuracy of morphometry of skull base and palate in gender discrimination using cone beam computed tomography (CBCT) scanning and to assess the accuracy of the results among a sample of the Arab population. Using CBCT scans, a cross-sectional analysis was conducted on 142 consented patients who underwent various dental procedures at the University Dental Hospital, Sharjah (UDHS). Of these patients, 70 were females and 72 were males, with respective means of 38.5 and 36.2 years. Eleven parameters related to skull base and palatal region were measured on the CBCT scans by two expert radiologists followed by statistical analysis. There was significant gender-based difference in the mean palatal width (PW) (p=0.001), mean palatal height (PH) (p=0.005). Among other skull base region parameters that were significant in term of gender-based difference like; the clivus length (CL) (p<0.001), occipital condyle height (OCH) (p<0.001), basal angle (BA) (p=0.006) and transverse diameter of foramen magnum (p=0.003). Only palate variables showed a significant age difference. Discriminant analysis related to gender showed that occipital condyle height was the most accurate and best discriminator among the skull base region parameters. The use of discriminant analysis in CBCT based on skull base and palatal region variables provides an efficient method for determining gender, which is particularly valuable in forensic science and anthropological research. Accurate gender identification is crucial in forensic investigations, and the skull base region, being a stable and sexually dimorphic anatomical feature, can serve as a reliable marker for this purpose.

Quaternary phosphonium AIEgens nanoparticles as innovative agents for developing latent fingerprints

Quaternary phosphonium salts, a significant category of organophosphorus compounds, have garnered substantial attention from chemists due to their wide range of applications across various research areas. These compounds are utilized in organic synthesis, catalysis, medicinal chemistry, natural materials, and coordination chemistry. Their versatility and effectiveness in these fields make them valuable tools in scientific research. Despite their extensive use in various applications, the potential of quaternary phosphonium compounds as fluorescent agents for revealing latent fingerprints (LFPs) remains largely unexplored, presenting an exciting opportunity for further research and development in forensic science. In this study, we designed molecules that combine the aggregation-induced emission (AIE) chromophore with triphenylphosphine to create a series of novel AIE amphiphiles, namely TPP1, TPP2, and TPP3. Through precise adjustment of the carbon chain length between the phenoxy group and the terminal triphenylphosphine, we were able to finely tune the nanostructures and hydrophobicity of the materials. TPP3 emerged as the optimal candidate, possessing the ideal particle size and hydrophobicity to effectively bind to LFPs, thus enabling efficient fingerprint visualization with enhanced fluorescence upon aggregation. Our findings introduce an innovative approach to fingerprint visualization, offering high selectivity, superior imaging of level 3 structures, and long-term effectiveness (up to 30 days). Additionally, TPP3's outstanding performance in imaging level 3 structures of LFPs is beneficial for analyzing incomplete LFPs and identifying individuals. By significantly improving the detection and analysis of LFPs, this approach ensures more accurate and reliable identification, making it invaluable for forensic investigations and security measures. The adaptability of these compounds to various fingerprint surfaces highlights their potential in diverse practical applications, enhancing their utility in both forensic science and security fields. This versatility allows for precise fingerprint visualization across different scenarios, making them a critical tool for advancing biometric and security technologies.

The impacts of thermocouple insulation failure on the accuracy of temperature measurement data in forensic fire-death scenarios-Part I: Physical disintegration.

Thermocouples are utilized to monitor a wide range of temperatures in industrial applications. They are also used in both fire and forensic science research to measure temperatures of fires and of materials exposed to fire. Taking accurate temperature measurements during forensic fire-death scenarios is very difficult due to direct fire exposure to thermocouples, shrinkage and destruction of tissues, and movements from pyre collapse and pugilistic posturing of human donors. This two-part study investigates the impacts on the accuracy of temperature data if the selected thermocouples are unable to withstand fire exposure. Part I (this article) provides an overview of thermocouple theory along with evidence of the physical deterioration that occurs when glass fiber-insulated thermocouple wires are overheated by exposure to fire-level temperatures in a muffle furnace. This study verified that insulation overheating causes embrittlement and disintegration, which can cause the indicated temperature to reflect a new location of measurement located far away from the original measuring junction at the thermocouple tip. Part II will discuss the measurement errors that occurred due to low electrical resistance of insulation when three different thermocouple models were passed through fire-level temperatures to measure an ice bath at a constant temperature of 0°C.

Signs of how the Sydney Declaration article is received in the forensic science literature

The Sydney Declaration is an initiative led by an informal group of forensic scientists with diverse backgrounds. It offers a vision of forensic science based on the trace, as a vestige of a past event related to security or a possible law violation. An article published in Forensic Science International (FSI) introduces to this view [1]. Our investigation delves into how the forensic science literature has received this article (the SD article), nearly two years after its publication. One of the main challenges of this exploratory study was to define the appropriate scope of forensic scientific literature, within which the SD article must be located. In general, the publishing domain is quickly evolving, with many competing players, while still being structured around standard academic disciplines. The forensic literature, meanwhile, is scattered and poorly connected. This reflects the fragmentation of practice and research in forensic science, and the difficulty of situating a scientific activity in such a way as to bring out its forensic substance. Nonetheless, the SD article fills a gap. By deciphering the critical concept of trace, it highlights how pivotal forensic science is in addressing societal challenges. Scholarly literature expresses clear quantitative interest in the SD article. It has received significant qualitative citations on multiple levels and dimensions, in a highly relevant manner and in accordance with its aim of providing a forensic foundation for various debates that have been conducted separately, notably over the last fifteen years.

Research on the Development of Methods for Detection of Substandard and Falsified Medicines by Clarifying Their Pharmaceutical Characteristics Using Modern Technology.

The existence of substandard and falsified medicines threatens people's health and causes economic losses as well as a loss of trust in medicines. As the distribution of pharmaceuticals becomes more globalized and the spread of substandard and falsified medicines continues worldwide, pharmaceutical security measures must be strengthened. To eradicate substandard and falsified medicines, our group is conducting fact-finding investigations of medicines distributed in lower middle-income countries (LMICs) and on the Internet. From the perspective of pharmaceutics, such as physical assessment of medicines, we are working to clarify the actual situation and develop methods to detect substandard and falsified medicines. We have collected substandard and falsified medicines distributed in LMICs and on the Internet and performed pharmacopoeial tests, mainly using HPLC, which is a basic analytic method. In addition to quality evaluation, we have evaluated the applicability of various analytic methods, including observation of pharmaceuticals using an electron microscope, Raman scattering analysis, near-IR spectroscopic analysis, chemical imaging, and X-ray computed tomography (CT) to detect substandard and falsified medicines, and we have clarified their limitations. We also developed a small-scale quality screening method using statistical techniques. We are engaged in the development of methods to monitor the distribution of illegal medicines and evolve research in forensic and policy science. These efforts will contribute to the eradication of substandard and falsified medicines. Herein, I describe our experience in the development of detection methods and elucidation of the pharmaceutical status of substandard and falsified medicines using novel technologies.

The Exploration of Age Related Mirna in Saliva Based On Massively Parellel Sequencing: A Pilot Study

Age determination is one of the key components of forensic science research.

Application of terahertz Time-Domain spectroscopy and chemometrics-based whale optimization algorithm in PDE5 inhibitor detection

Combating the illicit use of PDE5 inhibitor drugs is a focal point in forensic science research. In order to achieve rapid identification of such drugs, this study applies terahertz time-domain spectroscopy combined with chemometrics to establish a fast and accurate detection method for PDE5 inhibitors. The optimal detection method is determined by comparing the spectral performance of three optical parameters, namely absorption coefficient, refractive index, and dielectric constant. Linear discriminant models based on different spectral parameters, whale optimization algorithm optimized extreme learning machine models, and whale optimization algorithm optimized random forest models are established. The effectiveness and performance of principal component analysis and competitive adaptive reweighted sampling algorithm for spectral feature data selection are also investigated. The PDE5 inhibitor identification model based on the competitive adaptive reweighted sampling - whale optimization algorithm - random forest (CARS-WOA-RF) model achieves an accuracy of 98.61%, and the identification model for two concentrations of Sildenafil achieves 100% accuracy. The results demonstrate that terahertz time-domain spectroscopy combined with chemometrics can effectively detect various common types of PDE5 inhibitor drugs and different concentrations.

GeoFOR: A collaborative forensic taphonomy database for estimating the postmortem interval

Accurately assessing the postmortem interval (PMI), or the time since death, remains elusive within forensic science research and application. This paper introduces geoFOR, a web-based collaborative application that utilizes ArcGIS and machine learning to deliver improved PMI predictions. The geoFOR application provides a standardized, collaborative forensic taphonomy database that gives practitioners a readily available tool to enter case information that automates the collection of environmental data and delivers a PMI prediction using statistically robust methods. After case submission, the cross-validating machine learning PMI predictive model results in a R² value of 0.82. Contributors receive a predicted PMI with an 80% confidence interval. The geoFOR database currently contains 2529 entries from across the U.S. and includes cases from medicolegal investigations and longitudinal studies from human decomposition facilities. We present the overall findings of the data collected so far and compare results from medicolegal cases and longitudinal studies to highlight previously poorly understood limitations involved in the difficult task of PMI estimation. This novel approach for building a reference dataset of human decomposition is forensically and geographically representative of the realities in which human remains are discovered which allows for continual improvement of PMI estimations as more data is captured. It is our goal that the geoFOR data repository follow the principles of Open Science and be made available to forensic researchers to test, refine, and improve PMI models. Mass collaboration and data sharing can ultimately address enduring issues associated with accurately estimating the PMI within medicolegal death investigations.

DNA methylation-based age estimation and quantification of the degradation levels of bisulfite-converted DNA

DNA methylation modifications are known to influence epigenetic phenomena and have been a focus of forensic science research for some time. Degraded DNA after bisulfite treatment is widely used in DNA methylation analysis. In this study, we analyzed methylation levels at 12 CpG sites of four selected genomic regions by pyrosequencing after bisulfite treatment. DNA was extracted from buccal swab samples collected from 102 Japanese individuals who were 21–77 years old. We also developed a simple method to quantify the degradation levels of bisulfite-converted DNA by real-time PCR, and evaluated the effect of DNA degradation on age estimation. We found that the methylation levels and chronological ages were highly correlated in the four selected regions, and the mean absolute deviation (MAD) between chronological and estimated ages was low at 3.88 years. These results indicated that pyrosequencing analysis at the 12 CpGs was useful for age estimation in the Japanese population. To develop a sensitive quantification method, we analyzed the amplification efficiency of short and long fragments from 10 regions by real-time PCR. The amplification efficiency was highest for CCDC102B, and the degradation levels of bisulfite-converted DNA for the 102 samples were categorized as moderately or heavily degraded. For the younger age groups (20–49 years), the MADs were lower for moderately degraded DNA than they were for heavily degraded DNA. This finding indicates that degradation levels affected the accuracy of age estimation in most of the samples; the exception was the samples from the 50–77 years age group.

Aerobiology in Poland: Achievements and challenges

Aerobiology is a scientific discipline that focuses on investigating biological particles (bioaerosols) passively transported in the atmosphere, mainly fungal spores and pollen grains. Due to the allergic and pathogenic properties of these particles, aerobiological data is widely used in allergology, plant pathology, and agriculture. In addition, the results of aerobiological monitoring have found applications in biogeography, paleoecology, forestry, veterinary medicine, forensic science, and climate change research, as well as in the assessment of threats to the deterioration of cultural heritage. In this review, the contribution of Polish scientists to the development of worldwide aerobiology is presented. The most important results on the atmospheric dispersion of bioaerosols, spatiotemporal modeling, and novel methods used in aerobiological research are described. Moreover, the cooperation at the national and international levels as part of scientific programs and the perspectives for further development of aerobiology in Poland are discussed.

Non-destructive recognition of copy paper based on advanced spectral fusion and feature optimization

In order to provide more clues for ongoing investigations and case handling, as well as achieve fast, non-destructive, and accurate identification of copy paper found at crime scenes, this study aims to utilize advanced spectral fusion technology to characterize and identify the three-dimensional features of the “origin-manufacturer-brand” of copy paper. Confocal Raman Microscopic and Fourier transform infrared spectroscopy were employed to collect spectral data from 200 samples from four regions (Shandong, Henan, Shaanxi, Jiangsu). The effects of different preprocessing methods, such as Hilbert transformation and deconvolution, on the model's ability to distinguish were compared. Feature variables were extracted using principal component analysis, and Bayesian discriminant classification models were constructed based on single infrared spectroscopy, Raman spectroscopy, and three types of spectral fusion datasets. By comparing the classification accuracy of different models, the primary fusion based on the full spectrum dataset was selected as the optimal model for the three-dimensional feature classification of copy paper. The accuracy achieved for origin (96%), manufacturer (100%), and brand (100%) was satisfactory, and the classification results were highly accurate. This study provides valuable insights and serves as a reference for its application in forensic science research.

Towards more relevance in forensic science research and development

Many different issues have been identified in forensic science for more than 10 years. While quality management has often been suggested as a path forward, research is generally considered as an essential part of the solution. Through an overview of current forensic science research, this paper aims at evaluating if and how research answer the challenges forensic science is currently facing. While forensic related publications have massively increased over the years, approximately half of the publications were published in non-forensic sources, indicating that forensic science research tends to be led by other disciplines. Over the years, forensic science research has remained largely oriented towards methodological and technological development rather than relevance to the forensic science discipline and practice. Practical implementation of the techniques is rarely discussed from a forensic perspective, and thus research rarely move from the “proof-of-concept” stage to its utilisation in case investigation. The digital transformation also generated a massive increase of data, making it challenging to find the relevant pieces of information in the mass of “forensic” publications available on-line. Thus, we propose to refocus forensic science research on forensic fundamental and practical questions to strengthen the discipline and its impact on crime investigation and security issues. Our propositions represent an incentive to further discuss forensic science research and knowledge transmission through the definition of a common culture within the community, focusing on common fundamental knowledge such as a better understanding of the concept of trace and its case-based information content.

Metabolic profile determination of 2F-viminol - A novel synthetic opioid identified in forensic investigations.

Novel synthetic opioid (NSO) continue to emerge in the United States in the midst of an opioid crisis. The NSO 2F-viminol was identified in casework at the Center for Forensic Science Research and Education through its NPS Discovery program in 2019. Little information and published literature were available for this new opioid at the time. To address this, human liver microsomes (HLMs) were used to perform in vitro metabolism studies with a drug standard. The goal was to predict in vivo metabolism. Experimental samples were prepared using HLMs, NADPH, phosphate buffer (pH7.4), and a 2F-viminol standard. Standard samples were prepared containing only drug, control samples were prepared with drug and HLMs but no NADPH cofactor, and metabolism reaction mixtures contained drug, HLMs and NADPH. The subsequent mixtures were incubated with light shaking to allow metabolism to occur. After cleanup, metabolite mixtures were analyzed via a SCIEX TripleTOF 5600+ liquid chromatograph quadrupole-time-of-flight mass spectrometer (LC-QTOF-MS). The generated metabolic structures were elucidated using SCIEX MetabolitePilot software (version 2.0). In addition to remaining parent drug, seven metabolites of 2F-viminol were discovered, including N-dealkylated and hydroxylated species. The proposed primary metabolites of 2F-viminol were N-dealkylation (sec-butyl) + hydroxylation and N-dealkylation (sec-butyl); however, they should be confirmed in authentic samples, and forensic laboratories should consider adding 2F-viminol and its metabolites to screening protocols to help in extending the window of detection for the parent drug in toxicological samples. As NSOs continue to appear, forensic laboratories must continue metabolism experiments to generate information about pharmacokinetics.

DNA Tests Offer Clinching Proof in Awarding Capital Punishment for Rape and Murder of a 7-year-Old Girl

Forensic Science & Addiction Research DNA Tests Offer Clinching Proof in Awarding Capital Punishment for Rape and Murder of a 7-year-Old Girl Jeevanathan Arumugam*, Dhanapal Thilaga and Nithyandam Mahalakshmi Department of Forensic Sciences, DNA Division, India *Corresponding author: Jeevanathan Arumugam, Department of Forensic Sciences, DNA Division, Kamarajar Salai, India Submission: August 10, 2022;Published: August 24, 2022 DOI: 10.31031/FSAR.2022.06.000631 ISSN 2578-0042 Volume6 Issue1

The Subjective Experience of Personal Fulfillment in a Job with a Sense of Purpose

Forensic Science & Addiction Research The Subjective Experience of Personal Fulfillment in a Job with a Sense of Purpose Nestor Raul Porras Velasquez* Psychologist from the National University of Colombia, Researcher and independent profesional, Colombia *Corresponding author:Nestor Raul Porras Velasquez, Psychologist from the National University of Colombia, Researcher and independent professional, Colombia Submission: June 25, 2022;Published: July 22, 2022 DOI: 10.31031/FSAR.2022.05.000630 ISSN 2578-0042 Volume5 Issue5

Legal Regulation thе Forensic Activities in Digitalization

The article is devoted to the analysis some features of legal regulation of digitalization of forensic science. Some aspects of development of professional standards were also considered. The author examines in detail the main purposes of accreditation of testing and calibration laboratories. From a practical point of view, the shortcomings of some approaches to the legal regulation of digital traces research in forensic science.

Ethics reporting in forensic science research publications – A review

An essential element of compliance with ethical standards in scientific research is the reporting of a verifiable declaration of ethical approval and, when human subjects are involved - informed consent, in published works. The level of reporting of ethical permission for research published in forensic and investigative sciences journals has not been explored to date. Hence, we examined the reporting of ethical approval and informed consent in original research utilising human or animal subjects published in six forensic science journals from 2010 to 2019. We identified 10,192 articles and retained 3010 that satisfied the inclusion criteria of utilising human (91.2%), or animal (7.0%) or both (1.8%) subjects or tissues in experiments. Just over a third (1079/3010) of all studies declared obtaining ethical approval, with 927 (85.9%) of those indicating the name of the ethical committee, but only 392 (36%) provided an approval code. Furthermore, while consent was said to have been sought in 527 (17.5%) of studies, only 155 of those reported that written informed consent was obtained, eleven stated oral (verbal) consent, while the remaining 357 studies (67.7%) did not report the process used to gain consent. Ethical approval reporting rates differed between different research types, availability of financial support and whether authors were affiliated to academia or industry. The results demonstrate a low level of declaration of ethical approval and informed consent in forensic science research and publication, requiring urgent rectification. We support the adoption of the model proposed by Forensic Science International: Genetics as baseline recommendations to facilitate consistent nomenclature, transparency, and standard of ethical reporting in forensic science.

Barry Logan, a prominent toxicologist and forensic analytical chemist, kindly spoke with BrJAC about his research into drugs of abuse, the legacy and inspiration for future

Dr Barry Logan is a world leading forensic toxicologist currently serving as Chief Scientist at NMS Labs, and Executive Director at the Center for Forensic Science Research and Education (CSFRE) in Willow Grove, Pennsylvania. He was born and completed his undergraduate and graduate education in Glasgow, Scotland, completed a postdoctoral fellowship at the University of Tennessee in Memphis TN, then served for eighteen years as State Toxicologist for the State of Washington, with an appointment at the University of Washington in Seattle. For nine of those years he also served as Director of the Washington State Crime Laboratory System, which provided services in forensic biology, toxicology, chemistry, document examination, serology, DNA analysis, firearms and crime scene support. In 2008, Logan joined the United States leading forensic toxicology and chemistry reference laboratory - NMS labs - in Pennsylvania to direct their toxicology services. In 2010 he founded the CFSRE and in 2017, established www.NPSDiscovery.org the leading clearing-house for the dissemination of newly emergent drugs in the United States. He has over 150 publications and 600 presentations in forensic toxicology and analytical chemistry, including work on the effects of methamphetamine, cocaine and marijuana on drivers, and drug caused and related death. His recent work has focused on the analytical and interpretive toxicology of novel psychoactive substances (NPS). Dr Logan’s other appointments include Executive Director of the Robert F. Borkenstein course at Indiana University, and academic appointments at Arcadia University, and Thomas Jefferson University in Philadelphia. In recognition of his work and contributions, Dr. Logan has received numerous national and international awards, and in 2013-14 served as President of the American Academy of Forensic Sciences (AAFS). A recent bibliometric analysis of the impact of the world’s forensic scientists, positioned him as the leading contributor to research in the field of forensic toxicology in the United States, and sixth in the world. In the last ten years he has had extensive involvement with forensic scientists in Brazil, hosting graduate students from the Federal University of Rio Grande do Sul, USP, and Campinas University at his laboratory in the United States, and visiting scientists from the Federal Police and State Crime Laboratories. He has presented multiple times at Interforensics, ENQFor, and Brazilian Academy of Forensic Sciences meetings. The CSFRE supports participation of young scientists from Brazil in the AAFS meeting and a reciprocal opportunity for young US scientists to attend Interforensics.

Ignorance, the Hidden Epidemic Habits, Addiction and COVID 19

Forensic Science & Addiction Research Ignorance, the Hidden Epidemic Habits, Addiction and COVID 19 Santos Jose LF* FASTA University, Argentina *Corresponding author: Santos Jose LF, FASTA University, Avellaneda 3341, Mar del Plata, Argentina Submission: December 21, 2021;Published: January 11, 2022 DOI: 10.31031/FSAR.2022.05.000624 ISSN 2578-0042 Volume5 Issue4