Food Safety Agency Research Articles

Evidence of a positive association between dietary exposure to polychlorinated biphenyl (PCB) and weight gain among women in the E3N prospective cohort.

Polychlorinated biphenyls (PCB) and dioxins are persistent organic pollutants found in food and known for their ability to bioaccumulate. Various animal studies have highlighted obesogenic effects related to the exposure to PCB and dioxins, nevertheless human studies have led to inconsistent results. The present study aims to investigate the associations between dietary intakes of PCB and dioxins and the risk of weight gain, overweight and obesity in the French E3N cohort (Étude Épidémiologique auprès de femmes de la mutuelle générale de l'Éducation Nationale). The present study included 63,758 women with a mean age of 52.9 years, who completed a food frequency questionnaire in 1993 and were followed for 23 years. Dietary intakes to the sum of dioxins and dioxin-like PCB (DL-PCB) and to non-dioxin-like PCB (NDL-PCB) were estimated using food consumption data combined with food contamination levels from the French Agency for Food, Environmental and Occupational Health and Safety (ANSES). Adjusted Cox proportional hazard models were used to estimate hazard ratios (HR) and their 95 % confidence intervals for the risk of obesity, overweight and weight gain >10 kg during follow-up. Our results suggest a positive and linear association between dietary intakes of dioxins + DL-PCB and intakes of NDL-PCB and the risk of weight gain (HRdioxins+DL-PCB = 1.07 (1.05-1.1), HRNDL-PCB = 1.08 (1.06-1.1)), overweight (HRdioxins+DL-PCB = 1.03 (1.01-1.05), HRNDL-PCB = 1.05 (1.03-1.06)), and obesity (HRdioxins+DL-PCB = 1.08 (1.04-1.12), HRNDL-PCB = 1.09 (1.06-1.12)). In sensitivity analyses adjusting for diet, the association between dioxins + DL-PCB dietary intake and obesity was no longer observed. Results remained unchanged or were attenuated but still significant regarding the association between dietary intake of NDL-PCB and obesity risk in all sensitivity analyses. Similar results were observed for the risks of weight gain and overweight. This study suggests a positive and linear association between dietary intakes of NDL-PCB and the risk of obesity, a major risk factor for most non communicable diseases. Further studies are needed to confirm these results in other populations and to better understand the biological mechanisms underlying this association.

Urban food availability and consumption in Grand Lomé, Togo: A household perception analysis

Introduction: Food well-being of the population is one of the priorities of the Togolese government, which relies on the agricultural investment and food security Programme to increase national food production. In addition, the country relies on food imports to make up the shortfall. At the same time undernourishment and malnutrition remain high among the country’s population. This research analyzes food supply and its implications for household consumption in Grand Lomé, Togo. [Methods] The methodology used documents, a survey of 963 heads of household randomly sampled households and semi-structured interviews with 10 households and with Togolese food safety agency (ANSAT). Quantitative data were processed and analyzed using Excel spreadsheets R and R-Studio, while content analysis was applied to the verbal applied to the verbal statements collected. Results: Firstly, the results show that domestic agricultural production contributed an average of 91% of food supply between 2014–2017. The deficit is made up by food imports, which rose from 13.5% in 2014 to 15.4% in 2017. This translated into an acceptable food energy consumption of 2337 Kcal/head/day in 2017. Secondly, 81% of respondents recognize a strong food presence at consumer markets, except that the chi-square test applied to the data at the 5% threshold shows (p-value < 2.2 × 10−16), indicates that this satisfaction is a function of place of residence. Despite this, persistent shortages affect more staple crops, livestock and dairy products, leading households to deprive themselves and buy food at affordable prices. Finally, we observe non-diversified diets marked by regular consumption of “cereals/legumes”, vegetables and beverages to the detriment of “tubers/roots”, “meat/fish”, “fruit” and “dairy products”. Conclusion: This research shows that food supply, although adequate, is not sufficient to ensure balanced, nutritious and culturally appropriate food consumption by urban households. Recommendations: To meet these challenges, the central government, in collaboration with urban communes and consumer advocates, must mobilize resources to create urban agricultural farms, strengthen food protection systems, distribute staple products directly to households and limit the importation of food that is hazardous to health.

Human Exposure through the Diet to Arsenic and Other Toxic Elements: A Literature Review of Scientific Studies Conducted in Catalonia, Spain, in the Current Century.

Human exposure to arsenic and other toxic elements such as cadmium, lead and mercury may lead to a wide range of adverse health effects. In relation to this, it is well established that the diet is the main route of exposure to both essential and toxic trace elements. In recent years, the levels of toxic elements in foodstuffs have been measured in numerous studies conducted all over the world. Scientific databases show that, in the current century, China and Spain have been the countries where the most surveys on this topic have been carried out. Regarding Spain, Catalonia is the region where most studies aimed at determining the concentrations of trace elements in food have been performed. The objective of this paper was to review the studies carried out in Catalonia on the concentrations of As and toxic metals (including Cd, Hg and Pb) in food, as well as their estimated dietary intakes (EDIs). The results of total diet studies (TDSs) and duplicate diet (DD) studies have been included. For most toxic elements, a continued reduction in the EDI has been observed. This reduction is associated with a decrease in their concentrations in food, and with certain changes in dietary habits. Fish and seafood is the food group showing the highest content of toxic elements. However, none of the adult groups exceeded-in general-the safety thresholds for As, Cd, Hg and Pb established by the European Food Safety Agency (EFSA).

Dietary exposure to metals/metalloids and persistent organic pollutants in Spanish preschool and primary school children

Diet is a primary source of pollutant exposure. Given children's vulnerability to their effects, this study assessed dietary intake of metals/metalloids and different persistent organic pollutants in children in Tarragona (Spain), compared it with the health-based guideline values (HBGV), and identified the main dietary sources. The analysis included 533 preschoolers and 443 primary school children from the EPINED and ECLIPSES studies. Dietary intake of cadmium (Cd), methylmercury (MeHg), lead (Pb), inorganic arsenic (inAs), polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), dioxin-like polychlorinated biphenyls (DL-PCBs), and non-dioxin-like polychlorinated biphenyls (NDL-PCBs) was estimated using validated food frequency questionnaires and a database from the Catalan Agency for Food Safety. Preschoolers and primary school children exceeded the relevant reference value for InAs and DL-PCBs. Only preschoolers exceeded the limit for MeHg and PCDD/Fs. Cd, Pb, and NDL-PCBs intakes did not exceed the limits in either age group. Salted cereals/potatoes were the main source of InAs; fish for MeHg and DL-PCBs; and milk/yogurt for PCDD/Fs, followed by white fish in preschoolers and fatty fish in primary school children. The findings highlight that dietary exposure to some pollutants poses a significant risk to these children, emphasizing the need for public health policies to reduce pollutants in their diets.

Report of the Scientific Committee of the Spanish Agency for Food Safety and Nutrition (AESAN) on the programming of biological hazard sampling at official controls

Abstract The General State Administration establishes the food safety control and cooperation mechanisms with the competent authorities of the administrations responsible for official controls. In order to improve the quality and homogeneity of official controls related to biological hazards in food, the Spanish Agency for Food Safety and Nutrition (AESAN) and the autonomous communities have a Guidance document for the scheduling of biological hazard sampling within the framework of the National Plan for Official Control of the Food Chain 2021‐2025, which establishes a semi‐quantitative model that takes into account, on the one hand, the impact on health, considering incidence and severity, and, on the other, the prevalence, composed of data from non‐compliant samples and alert notifications. At AESAN's request, the Scientific Committee has assessed this Guidance document, and the final conclusion is that this Guidance document is suitable at the present time, for the intended purpose. Specifically, the criteria based on the calculation of the health impact are considered valid for the biological hazards studied. Regarding the prevalence calculation, different percentile levels could be considered for the parameters of percentage of non‐compliant samples and number of alert notifications. The use of the correction factor for inactivating treatment for the correction of the score associated with prevalence is positively assessed. Regarding the distribution of food categories and hazards analysed, it is considered suitable and it is recommended to re‐evaluate this distribution considering average consumption data of the different food categories in each of the autonomous communities. The procedure to calculate the number of samples and the risk score intervals used for the different hazard‐food pairs is also considered suitable for the intended purpose. Finally, some suggestions are made for revising some criteria in the future in the event that information is available, and it is indicated that the Guidance document should be updated periodically in the light of the experience of its application, progress in scientific knowledge, changes in legislation and guidelines and tools on prioritisation and frequency of risk‐based inspection that may be developed at national or European Union level.

Report of the Scientific Committee of the Spanish Agency for Food Safety and Nutrition (AESAN) on the risk associated with the consumption of food supplements that contain creatine as an ingredient

Abstract Creatine is an endogenous substance synthesised in the pancreas, kidneys and liver, which can also be provided through the diet, mainly through the intake of meat and fish, or in the form of a food supplement ingredient, with creatine monohydrate and creatine hydrochloride being the most common marketed forms of creatine. Creatine is effective in improving the training and performance of short duration and high intensity physical exercise. In fact, Regulation (EU) No. 432/2012 includes the health claims attributable to foods that provide a daily intake of 3 g of creatine and related to the effect of creatine on physical exercise. Based on the information currently available on creatine and considering that creatine monohydrate and hydrochloride are the forms of creatine commonly used in the manufacture of food supplements with creatine, the Scientific Committee considers that the maximum daily amounts of 3.41 g of creatine monohydrate and 3.84 g of creatine hydrochloride provide a maximum daily amount of 3 g/day of creatine and are acceptable from the standpoint of their safety in use as food supplements for a healthy adult population.

Accurate determination of three new antihypertensive drugs illegally added to food using ultra-high performance liquid chromatography-tandem mass spectrometry

Effective strategies are required to address food safety issues related to the illegal addition of antihypertensive drugs to food and claims of antihypertensive function. In this study, a novel ultra-high performance liquid chromatography-triple-quadrupole mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of three antihypertensive drugs (azilsartan, candesartan cilexetil, and lacidipine) in 12 food matrices (pressed candies, solid beverages, alternative teas, tea drinks, biscuits, jellies, mixed liquors, oral liquids, medicinal teas, tablets, hard capsules, and soft capsules). Initially, mass spectrometry parameters, such as the collision energies of the three antihypertensive drugs, were optimized. Subsequently, the response intensities and chromatographic separation conditions of the three drugs in different mobile phases were compared. In addition, to enhance the recoveries, various extraction solvents and purification methods, including solid-phase extraction (SPE) columns and the QuEChERS technique, were investigated. In the developed method, sample determination involved three steps. First, the sample was extracted using 0.2% (v/v) formic acid in acetonitrile and then filtered using high-speed centrifugation, in addition, the extracted solution of alternative tea and medicinal tea was purified using the QuEChERS technique. Second, the supernatant was diluted with water, and filtered through a 0.22 μm polytetrafluoroethylene (PTFE) membrane. Finally, the analytes were separated on an Agilent Eclipse Plus RRHD C18 column (50 mm×2.1 mm, 1.8 μm) using a 5 mmol/L ammonium formate aqueous solution and acetonitrile as the mobile phases under gradient elution conditions and then detected using UHPLC-MS/MS with positive electrospray ionization (ESI) in the multiple reaction monitoring (MRM) mode. Quantitative analysis was performed using a matrix-matched external standard method. Methodological validation showed good linear relationships for all three antihypertensive drugs in the investigated concentration ranges, with correlation coefficients (r2) greater than 0.996. The limit of detection (LOD) and limit of quantification (LOQ) of lacidipine were 0.02 mg/kg and 0.04 mg/kg, respectively, whereas those of the other two drugs were 0.01 mg/kg and 0.02 mg/kg, respectively. In the 12 food matrices, the average recoveries of the drugs ranged from 86.6% to 107.5% with relative standard deviations (RSDs) of 1.1%-10.9% (n=6) at low, medium, and high spiked levels. Furthermore, this method was successfully applied to the analysis of real food samples. Overall, the newly developed method is simple, rapid, sensitive, accurate, and suitable for the qualitative and quantitative determination of antihypertensive drugs in different food matrices. This work could provide technical support for food safety agencies in implementing measures against the illegal addition of antihypertensive drugs to food.

A foodborne outbreak caused by staphylococcal enterotoxins in cheese sandwiches in northern Italy

Staphylococcus aureus can produce numerous toxins, including staphylococcal enterotoxins, namely, SEs, SEA to SEE, SEG to SEI, and SER to SET. They have demonstrated emetic activity and can cause food poisoning outbreaks (FBOs). Here, we report a multidisciplinary investigation conducted by authorities competent in food safety in collaboration with veterinary and health and hygiene services. The issue was foodborne intoxication which involved eight members (aged 12–74 years) of the same family. Notification reported that time to symptom onset was about 3 hours after lunch, which suggests that the food contained preformed toxins. Ham and cheese used for the preparation of sandwiches consumed by all symptomatic relatives were suspected to be the source of FBO. The day after symptoms appeared, samples were collected at the restaurant and delivered to the food safety laboratory. All analyses performed on official food samples are validated according to ISO 17025:2017 and accredited. The cheese subsamples were all positive for coagulase-positive staphylococci CPS (range: 1.1*103 CFU/g to 8.1*103 CFU/g). The pooled sample tested positive for staphylococcal enterotoxin D (SED) at 0.649 ng/g. This concentration can cause symptoms of FBO. Following the notification of suspected food poisoning, a rapid response by the district food safety agency is necessary. In the case described here, the multidisciplinary collaboration facilitated the collection of samples at the food business plant aimed at identifying a suspected source of intoxication and to withdraw unsafe food from the market.

Comparative study of dietary selenium sources on gilthead seabream (Sparus aurata): Growth, nutrient utilization, stress response and final product quality

Selenium (Se) is a crucial trace mineral in human nutrition, known for its various health benefits, including the enhancement of antioxidant defenses. Enrichment of fish fillets with Se is seen as an interesting strategy to reduce the risk of Se deficiency in human diets. While inorganic sources have been traditionally used to supplement Se in fish diets, organic Se forms have demonstrated superior bioavailability and efficacy in managing oxidative stress and tissue retention rates compared to their inorganic counterparts.The main objective of this study was to assess the performance, stress resistance, and product quality of gilthead seabream (Sparus aurata) in response to diets supplemented with 0.2 mg kg−1 of Na2SeO3 (CTRL), Zn-L-SeMet (AVSe) or OH-Se-Met (ORGSe), complying therefore with the European Food Safety Agency (EFSA) maximum allowed supplementation of organic Se (0.2 mg kg−1). Diets were formulated to be similar in their protein (48% DM) and lipid content (17% DM), and hand-fed to apparent satiety to quadruplicate groups of 20 fish (initial body weight ∼ 75 g) gilthead seabream juveniles for 137 days. Fish were kept in a recirculating saltwater system (salinity 35‰, 22 ± 1 °C) until they reached commercial size (>250 g). At the end of the growth trial, all fish were individually weighed and measured, with 4 fish per tank sampled for whole-body composition analysis. Fillet yield, muscle lipid and mineral composition, and hepatic oxidative stress indicators were assessed (n = 12). Lipid peroxidation, muscle pH, water-holding capacity (WHC), color, and texture were evaluated on days 7 and 14 (n = 8), while sensory analysis was conducted on 20 fish. Additionally, 12 fish per treatment were subjected to overcrowding (100 kg m−3) for 5 min and air exposure for 1 min. After a 1-h recovery period, plasma and liver samples were collected from these fish, and results were compared to those of the non-stress group. All diets yielded equivalent growth performance, ensuring low feed conversion ratio. Fish fed the AVSe diet exhibited higher whole-body protein content compared to those fed the CTRL diet. Regarding mineral utilization, increased Se retention and gain in fish fed AVSe and ORGSe diets led to higher Se content in these fish compared to the CTRL. Additionally, the AVSe diet has also resulted in significantly increased Se deposition in the muscle compared to the other tested Se sources, contributing with 34% of the Se Daily Recommended Intake (DRI). The assessed flesh quality parameters and overall consumer acceptance did not reveal significant differences among the groups. Exposure to an acute stress was found to decrease the activities of Glutathione reductase and glutathione peroxidase, which in turn has increased the level of oxidized glutathione, particularly in the AVSe group. Compared to OH-Se-Met and Na2SeO3, Zn-L-SeMet was more effective in promoting response to stress and nutritional value of gilthead seabream as an important source of protein and Se in human diet.

Assessing refrigerated preservation performance using Listeria predictive microbiology models and temperature data: Refrigerator performance indicator and time-temperature equivalent.

Time-temperature data for queso fresco (QF) cheese varieties stored in a residential refrigerator operating at 5°C and a predictive microbiology secondary model for Listeria monocytogenes in QF were used to estimate a refrigerator performance indicator (RPI) of microbial preservation. RPI values were used to assess how compressor technology (single [SS] and variable speed [VS]), ambient temperature (21.1°C [LT] and 32.2°C [HT]), and refrigerator load (22.5kg regular load and 39kg higher load) affected preservation performance. All deterministic and probabilistic RPI estimations slightly exceeded the desirable 1.0 value, i.e., the variable temperatures for the QF kept in the refrigerator were worse than keeping it constantly at the temperature recommended by food safety agencies for QF. Furthermore, the mean comparison of estimates of the time-temperature equivalent indicator previously developed by French researchers showed similar behavior to those observed for RPI. Finally, statistical analysis showed that Tambient was the factor with the highest impact on refrigerator performance because of its impact on the sample temperature increase during door openings and when exposed to ambient temperature during product use. This highlights the need to reduce the time for product temperature recovery by improving the compressor operation logic. Also important are consumer behavior changes such as a reduction in product exposure to ambient temperature and in the door opening duration and frequency. PRACTICAL APPLICATION: This study demonstrated how a quantitative tool (RPI) can assess refrigerator preservation performance. Although the findings presented can be applied to any cold chain segment, the data used was collected for its weakest link, the domestic refrigerator. Surveys show that 77% of them operate above the recommended 4°C. The RPI methodology is ready for use by refrigerator designers to assess performance improvements possible by modifications of the compressor operation logic. Moreover, it can be integrated into smart-hubs monitoring the frequency and duration of refrigerator door openings to inform consumers when their habits are compromising the preservation performance of the refrigerator.

Reviewer Acknowledgements for Journal of Food Research, Vol. 13 No. 2

Journal of Food Research wishes to acknowledge the following individuals for their assistance with peer review of manuscripts for this issue. Their help and contributions in maintaining the quality of the journal are greatly appreciated. Journal of Food Research is recruiting reviewers for the journal. If you are interested in becoming a reviewer, we welcome you to join us. Please contact us for the application form at: jfr@ccsenet.org Reviewers for Volume 13, Number 2 Diego A. Moreno-Fernández, CEBAS-CSIC, Spain Elke Rauscher-Gabernig, Austrian Agency for Health and Food Safety, Austria Lenka Kourimska, Czech University of Life Sciences Prague, Czech Republic Liana Claudia Salanta, University of Agricultural Sciences and Veterinary Medicine, Romania Mehana E. E. Hamouda, Alexandria university, Egypt Rania I.M. Almoselhy, Agricultural Research Center, Egypt Rozilaine A. P. G. Faria, Federal Institute of Science, Education and Technology of Mato Grosso, Brazil

Knowledge, perception, and practical understanding of food labels: A cross-sectional study among Bangladeshi consumers.

This research aimed to assess consumers' knowledge, perception, and practical understanding of food labels. A validated, structured questionnaire was employed for data collection. Data were collected from 1238 respondents covering all eight administrative divisions of Bangladesh using a nonrandomized convenience sampling method. Linear regression analyses were conducted to establish the relationship between demographic attributes and respondents' practical understanding of food labels. The majority of participants (52.5%) actively read food labels when purchasing a product for the first time. Food labels are regarded as "very important" by 56.2% of respondents and "important" by 35.7% of respondents. Label information is prioritized to highlight the importance of clear production and expiration dates (70%), followed by nutritional composition (56.7%) and source of raw material (52.5%). Despite this, over half of the participants demonstrated a limited practical understanding of the nutritional components on labels. The results of our linear regression analysis suggest that individuals within the age range of 18-28, belonging to Muslim communities, residing in the Dhaka and Khulna divisions, being higher educated, possessing a good understanding of nutrition, and being acquainted with the food safety agency of Bangladesh tend to exhibit a greater degree of practical knowledge regarding food labeling when compared to their counterparts. The study emphasizes the importance of clearer label information, particularly for nutritional value, and calls for targeted educational programs to improve consumer understanding of food labels, with a focus on older age groups and expanded educational efforts.

Campylobacter assessment along the Spanish food chain: Identification of key points.

Campylobacteriosis, caused by Campylobacter spp., is one of the most important foodborne zoonotic diseases in the world and a common cause of gastroenteritis. In the European Union, campylobacteriosis is considered the most common zoonotic disease, with over 10,000 cases in 2020 alone. This high occurrence highlights the need of more efficient surveillance methods and identification of key points. Herein, we evaluated and identified key points of Campylobacter spp. occurrence along the Spanish food chain during 2015-2020, based on the following variables: product, stage and region. We analysed a dataset provided by the Spanish Agency for Food Safety and Nutrition using a machine learning algorithm (random forests). Campylobacter presence was influenced by the three selected explanatory variables, especially by product, followed by region and stage. Among the studied products, meat, especially poultry and sheep, presented the highest probability of occurrence of Campylobacter, where the bacterium was present in the initial, intermediate and final stages (e.g., wholesale, retail) of the food chain. The presence in final stages may represent direct consumer exposure to the bacteria. By using the random forest method, this study contributes to the identification of Campylobacter key points and the evaluation of control efforts in the Spanish food chain.

Scientific opinion on the ANSES analysis of Annex I of the EC proposal COM (2023) 411 (EFSA-Q-2024-00178).

EFSA was asked by the European Parliament to provide a scientific opinion on the analysis by the French Agency for Food, Environmental and Occupational Health & Safety (ANSES) of Annex I of the European Commission proposal for a regulation 'on plants obtained by certain new genomic techniques (NGTs) and their food and feed, and amending regulation (EU) 2017/625'. The Panel on genetically modified organisms (GMO) assessed the opinion published by ANSES, which focuses on (i) the need to clarify the definitions and scope, (ii) the scientific basis for the equivalence criteria and (iii) the need to take potential risks from category 1 NGT plants into account. The EFSA GMO Panel considered the ANSES analysis and comments on various terms used in the criteria in Annex I of the European Commission proposal and discussed definitions based on previous EFSA GMO Panel opinions. The EFSA GMO Panel concluded that the available scientific literature shows that plants containing the types and numbers of genetic modifications used as criteria to identify category 1 NGT plants in the European Commission proposal do exist as the result of spontaneous mutations or random mutagenesis. Therefore, it is scientifically justified to consider category 1 NGT plants as equivalent to conventionally bred plants with respect to the similarity of genetic modifications and the similarity of potential risks. The EFSA GMO Panel did not identify any additional hazards and risks associated with the use of NGTs compared to conventional breeding techniques in its previous Opinions.

Clinically Vulnerable Groups in Foodborne Disease Prevalence and Definitions Across Global Food Safety Agencies

Clinically Vulnerable Groups in Foodborne Disease Prevalence and Definitions Across Global Food Safety Agencies

Plant‐based meat analogues marketed in Catalonia: analysis of contaminants, essential elements, microbiological and physicochemical status, dietary intake estimation and health risk assessment

Abstract The latest barometer of food safety in Catalonia conducted by the Catalan Food Safety Agency (ACSA for its initials in Catalan) in 2021 shows that 28.7% of the population consumes meat analogues (substitutes). The main reasons for consuming this type of food are animal welfare, followed by environmental and health reasons. Due to the increasing demand for plant‐based meat analogues, the number of such products available on the market has also increased. Currently, however, there is a lack of knowledge regarding the composition of meat analogue products and their impact on human health. In this context, it was deemed necessary to complement the first report by the ACSA Scientific Advisory Committee on Food Safety with the present and more specific study on the content of contaminants and essential elements, several microbiological parameters and physicochemical characteristics of plant‐based meat analogues in order to assess the population of Catalonia's exposure to their potential health risks. The results indicate that the content of essential elements (Ca, Cr, Fe and Zn) and contaminants (As, Cd, Hg, Pb and Ni) in the plant‐based meat analogues are mostly of the same order of magnitude as those observed in the meat products evaluated in the total diet studies. The estimated dietary intakes for the evaluated population groups show values well below the toxicological safety values established for the different contaminants. Only for nickel could the risk assessment with regard to systemic contact dermatitis indicate a potential health problem for nickel‐sensitised individuals. The estimated dietary intake of essential elements associated with the consumption of one serving (100 g) of meat analogues hamburgers, or sausages, or meatballs or vegetable strips represents between 6.9 and 67.7% of the established recommended daily intake values. Out of the total number of samples evaluated, “Salmonella spp.” was detected in 6 samples. “Listeria monocytogenes” was detected by PCR in 3 samples; quantification was always lower than 10 cfu/g. The physicochemical characteristics showed very similar values in all meat analogues samples. The observed water activity and pH values favour the growth of microorganisms. To ensure the safety of products it is paramount that the need to subject the food to a complete heat treatment prior to consumption is highlighted on the labelling.

Wastewater-based epidemiology methodology to investigate human exposure to bisphenol A, bisphenol F and bisphenol S.

Wastewater-based epidemiology (WBE) has become an invaluable tool for tracking the evolution of use or exposure of/to numerous substances. Bisphenols, commonly utilized in manufacturing plastic goods, have been categorized as endocrine disrupting chemicals, underscoring the critical need for real-time data on their local-level exposure to safeguard public health. In this study, we have developed a novel analytical method and WBE framework for the assessment of population-level exposure to bisphenol A (BPA) and its most prominent substitutes, bisphenols F and S (BPF and BPS), through the determination their Phase II metabolites in wastewater by WBE. Stability and exclusivity tests denoted that glucuronides are not stable in sewage, whereas sulfate metabolites are good biomarkers. Therefore, a solid-phase extraction followed by liquid chromatography-tandem mass spectrometry method was developed for the bisphenols’ monosulfates and BPA bissulfate. The analytical method was validated with three different wastewater matrices, providing trueness (as recovery) in the 79–112 % range with relative standard deviations < 12 %, and method quantification limits below 2 ng L−1 for monosulfates, but higher (35 ng L−1) for BPA bissulfate. Subsequently, the method was applied to 24h-composite raw wastewater samples collected over a week in 4 different locations in Spain and Portugal. BPA bissulfate was not detected, but the three monosulfate metabolites of each bisphenol were positively detected in the samples, being the metabolite of BPA the most prevalent, followed by those of BPF and BPS. Community-wide BPA intake was then estimated to be higher than the European Food Safety Agency (EFSA) tolerable daily intake (TDI) of 2 × 10−4 µg kg−1day−1 in all locations. In the case of BPF and BPS, there is not enough metabolism data or even established limit, but they would also surpass safe levels in several locations if a similar metabolism and TDI would be assumed. This innovative method could be used to a larger set of wastewater-treatment plants as an early-warning approach on human exposure to bisphenols.

Determination of the urinary concentrations of six bisphenols in public servants by online solid-phase extraction-liquid chromatography tandem mass spectrometry

Bisphenols are widely used as monomers and additives in plastic production. Thus, bisphenol A (BPA) and its most prominent substitutes have been detected in many environmental and human samples. This study proposes an online solid-phase extraction analytical methodology coupled to liquid chromatography with tandem mass spectrometry for the determination of six bisphenols (BPA and bisphenols F (BPF), S (BPS), AF (BPAF), B (BPB), and E (BPE)) in urine samples as an efficient and automated methodology. The method was developed and validated for all bisphenols with good recoveries (92–112%) and repeatability (RSD ≤ 10%) despite the variable matrix effects, except BPAF (which would require a dedicated internal standard), achieving method quantification limits in the 0.05–2.2 ng mL−1 range. The methodology was subsequently applied to 435 urine samples from a non-occupational exposure population (civil servants for the regional government) from Santiago de Compostela (Galicia, Spain). Only BPA, BPF, and BPS were positively detected; the last two presented higher detection frequencies than BPA. When the urinary concentrations are extrapolated to human intake and compared to the European Food Safety Agency (EFSA) tolerable daily intake (TDI) of 2 × 10−4 µg kg−1 day−1 (TDI), all BPA positively identified samples would surpass this threshold. Although no TDI exists currently for the other two identified bisphenols, it is evident that human exposure to bisphenols should be limited. Finally, the results stratification by gender revealed higher levels of exposure to BPF in the women group.

Association of Prenatal Dietary Toxicants and Inorganic Arsenic Exposure with Children's Emotional and Behavioral Problems: ECLIPSES Study.

Prenatal exposure to dietary toxicants is linked to neurocognitive issues, but its effect on early emotional and behavioral development in children is less clear. To explore the relationship between prenatal intake of As, iAs, Cd, MeHg, Pb, PCDD/Fs, DL-PCBs, and NDL-PCBs and emotional and behavioral issues in four-year-old children. This study included 192 mother-child pairs from the ECLIPSES study, assessing prenatal dietary toxicant exposure through a food-frequency questionnaire and Catalan Food Safety Agency data. Children's emotional and behavioral scores were evaluated using the Child Behavior Checklist for ages 1.5-5 years. Multivariable regression and logistic models were used, focusing on iAs after finding significant preliminary associations. Increased prenatal dietary intake of iAs was associated with internalizing, externalizing, and attention-deficit/hyperactivity problems. Higher iAs levels (>4.16 μg/day) significantly increased the risk of total problems (OR = 2.94) and specific issues like anxious/depressed (OR = 4.88), anxiety (OR = 3.27), and oppositional defiant problems (OR = 4.30). High iAs consumption correlated with the intake of meat, eggs, cereals, tubers, fruits, and pulses Prenatal dietary iAs exposure is associated with various emotional and behavioral problems in children. Monitoring and reducing iAs levels in food are crucial for public health.

Addressing the safety of new food sources and production systems.

New food sources and production systems (NFPS) are garnering much attention, driven by international trade, changing consumer preferences, potential sustainability benefits, and innovations in climate-resilient food production systems. However, NFPS can introduce new challenges for food safety agencies and food manufacturers. Most food safety hazards linked to new foods have been identified in traditional foods. However, there can be some food safety challenges that are unique to new foods. New food ingredients, inputs, and processes can introduce unexpected contaminants. To realize the full potential of NFPS, there is a need for stakeholders from governments, the food industry, and the research community to collectively work to address and communicate the safety of NFPS products. This review outlines known food safety hazards associated with select NFPS products on the market, namely, plant-derived proteins, seaweeds, jellyfish, insects, microbial proteins, as well asfoods derived from cell-based food production, precision fermentation, vertical farming, and 3D food printing. We identify common elements in emerging NFPS regulatory frameworks in various countries/regions. Furthermore, we highlight current efforts in harmonization of terminologies, use of recent scientific tools to fill in food safety knowledge gaps, and international multi-stakeholder collaborations to tackle safety challenges. Although there cannot be a one-size-fits-all approach when it comes to the regulatory oversight for ensuring the safety of NFPS, there is a need to develop consensus-based structured protocols or workflows among stakeholders to facilitate comprehensive, robust, and internationally harmonized approaches. These efforts increase consumers' confidence in the safety of new foods and contribute toward fair practices in the international trade of such foods.