Food Hoarding Behavior Research Articles

Blunted food conditioned place preference-like behavior in adolescent-stressed male hamsters

Social stress during adolescence results in long lasting weight gain, obesity, and enhanced food hoarding behavior in hamsters. We wanted to determine whether stress also enhanced conditioned place preference-like behavior (CPP-like) for food reward, as would be expected from studies with substances like cocaine. Our experimental animals were exposed daily to aggressive adults for two weeks in early puberty, while also trained to explore a V-shaped maze containing a food reward at one end. They were tested for CPP-like behavior on the last day of social stress. Our results showed that while stress enhanced weight gain, food intake, food efficiency, and body fat, it caused a reduction of Place Preference as compared to controls. In fact, the correlated relationship between Place Preference and body fat was inverted by stress exposure: while it was positively correlated in controls, it was mildly negatively correlated in stressed hamsters. These unexpected data illustrate the extent of adaptive behavior in foraging animals once a resource has become untrustworthy.

High-tannin food enhances spatial memory and scatter-hoarding in rodents via the microbiota-gut-brain axis

BackgroundThe mutually beneficial coevolutionary relationships between rodents and plant seeds have been a theme of research in plant-animal relationships. Seed tannins are important secondary metabolites of plants that regulate the food-hoarding behavior of rodents; however, the underlying molecular mechanisms are not yet clear. In this study, we investigated whether and how seed tannins improve spatial memory and regulate the hoarding behavior of Tamias sibiricus by altering their gut microbiota.ResultsWe showed that acorn tannins not only improved spatial memory but also enhanced scatter-hoarding in T. sibiricus. Changes in the composition and function of the gut microbiota in response to tannins from acorns are closely related to these improvements. Metabonomic analyses revealed the role of gut isovaleric acid and isobutyric acid as well as serum L-tryptophan in mediating the spatial memory of T. sibiricus via the gut microbiota. The hippocampal proteome provides further evidence that the microbiota-gut-brain axis regulates spatial memory and scatter-hoarding in animals. Our study is likely the first to report that plant secondary metabolites improve hippocampal function and spatial memory and ultimately modulate food-hoarding behavior via the microbiota-gut-brain axis.ConclusionOur findings may have resolved the long-standing puzzle about the hidden role of plant secondary metabolites in manipulating food-hoarding behavior in rodents via the microbiota-gut-brain axis. Our study is important for better understanding the mutualistic coevolution between plants and animals.-v6UxNpVaqDZi2u857RxwnVideo

Pilfering personalities: Effects of small mammal personality on cache pilferage.

Small mammals such as mice and voles play a fundamental role in the ecosystem service of seed dispersal by caching seeds in small hoards that germinate under beneficial conditions. Pilferage is a critical step in this process in which animals steal seeds from other individuals' caches. Pilferers often recache stolen seeds, which are often pilfered by new individuals, who may recache again, and so on, potentially leading to compounded increased dispersal distance. However, little research has investigated intraspecific differences in pilfering frequency, despite its importance in better understanding the role of behavioural diversity in the valuable ecosystem service of seed dispersal. We conducted a field experiment in Maine (USA) investigating how intraspecific variation, including personality, influences pilferage effectiveness. Within the context of a long-term capture-mark-recapture study, we measured the unique personality of 3311 individual small mammals of 10 species over a 7-year period. For this experiment, we created artificial caches using eastern white pine (Pinus strobus) seeds monitored with trail cameras and buried antennas for individual identification. Of the 436 caches created, 83.5% were pilfered by 10 species, including deer mice ((Peromyscus maniculatus) and southern red-backed voles (Myodes gapperi). We show how individuals differ in their ability to pilfer seeds and that these differences are driven by personality, body condition and sex. More exploratory deer mice and those with lower body condition were more likely to locate a cache, and female southern red-backed voles were more likely than males to locate caches. Also, caches were more likely to be pilfered in areas of higher small mammal abundance. Because the risk of pilferage drives decisions concerning where an animal chooses to store seeds, pilferage pressure is thought to drive the evolution of food-hoarding behaviour. Our study shows that pilferage ability varies between individuals, meaning that some individuals have a disproportionately strong influence on others' caching decisions and disproportionately contribute to compounded longer-distance seed dispersal facilitated by pilferage. Our results add to a growing body of knowledge showing that the unique personalities of individual small mammals play a critical role in forest regeneration by impacting seed dispersal.

Conspecific density drives sex-specific spatial wintertime distribution and hoarding behaviour of an avian predator

Most studies on intraspecific competition, i.e., competition among individuals of the same species, have been conducted during the breeding season. Yet, at northern latitudes, intraspecific competition is expected to be particularly strong under the harsh weather conditions of the non-breeding season with limited number of resources available per individual. We studied the food-hoarding behaviour of wintering Eurasian Pygmy Owls (Glaucidium passerinum) along with sex- and age-specific spatial distribution in relation to fluctuating main prey abundance (voles) and conspecific density using a 15-year dataset. In low vole abundance years, increasing conspecific density reduced the total prey number stored by an owl, suggesting high costs of exploitative competition. The distance between the stores of nearest neighbours was greater when both were females, suggesting that the spatial avoidance is driven by sex-specific competition. However, food stores of females had a larger amount of prey items, especially when the nearest neighbour was of the same sex. The number of stores hoarded by an owl increased with increasing conspecific densities. Distributing the prey items to multiple store-sites instead of one (shifting from larder-hoarding towards scatter-hoarding) can help to reduce the overall loss to potential pilfering when conspecific density is high. These results combined suggest that high conspecific density inflames sex-specific interference competition, rather than solely exploitative competition, and in turn drives the observed sex-specific spatial distribution. Adopting a sex-specific spatial distribution according to hoarding and aggressive behaviour can be a way to reduce the severity of intraspecific competition locally and could have cascading effects on the prey community.

Behavioral adaptation of sympatric rodents to early germination of oak acorns: radicle pruning and embryo excision.

The seed germination schedule is a key factor affecting the food-hoarding behavior of animals and the seedling regeneration of plants. However, little is known about the behavioral adaptation of rodents to the rapid germination of acorns. In this study, we provided Quercus variabilis acorns to several rodent species to investigate how food-hoarding animals respond to seed germination. We found that only Apodemus peninsulae adopted embryo excision behavior to counteract seed germination, which is the first report of embryo excision in nonsquirrel rodents. We speculated that this species may be at an early stage of the evolutionary response to seed perishability in rodents, given the low rate of embryo excision in this species. On the contrary, all rodent species preferred to prune the radicles of germinating acorns before caching, suggesting that radicle pruning is a stable and more general foraging behavior strategy for food-hoarding rodents. Furthermore, scatter-hoarding rodents preferred to scatter-hoard and prune more germinating acorns, whereas they consumed more nongerminating acorns. Acorns with embryos excised rather than radicles pruned were much less likely to germinate than intact acorns, suggesting a behavioral adaptation strategy by rodents to the rapid germination of recalcitrant seeds. This study provides insight into the impact of early seed germination on plant-animal interactions.

Assessment of Repetitive and Compulsive Behaviors Induced by Pramipexole in Rats: Effect of Alpha-Synuclein-Induced Nigrostriatal Degeneration.

Treatment with dopamine agonists in Parkinson’s disease (PD) is associated with debilitating neuropsychiatric side-effects characterized by impulsive and compulsive behaviors. The vulnerability to develop such impairments is thought to involve interactions between individual vulnerability traits, types of antiparkinsonian medications, and the neurodegenerative process. We investigated the effect of the dopamine D3/D2 agonist pramipexole (PPX) and selective nigrostriatal degeneration achieved by viral-mediated expression of alpha-synuclein on the expression of repetitive and compulsive-like behaviors in rats. In a task assessing spontaneous food hoarding behavior, PPX increased the time spent interacting with food pellets at the expense of hoarding. This disruption of hoarding behavior was identical in sham and lesioned rats. In an operant post-training signal attenuation task, the combination of nigrostriatal lesion and PPX decreased the number of completed trials and increased the number of uncompleted trials. The lesion led to an increased compulsive behavior after signal attenuation, and PPX shifted the overall behavioral output towards an increased proportion of compulsive lever-presses. Given the magnitude of the behavioral effects and the lack of strong interaction between PPX and nigral degeneration, these results suggest that extra-nigral pathology may be critical to increase the vulnerability to develop compulsive behaviors following treatment with D3/D2 agonists.

Influences of News and Social Media on Food Insecurity and Hoarding Behavior During the COVID-19 Pandemic.

The aim of this study was to examine how sociodemographic variables and frequency of media consumption affect hoarding behavior and food insecurity concerns during the coronavirus disease 2019 (COVID-19) pandemic. A quantitative, nonexperimental, correlational online survey was administered using a convenience sample of 203 participants from the United Kingdom with no medical issues that affected buying behavior during the pandemic to examine perceptions related to food insecurity, and self-reported food hoarding behavior. Younger adults and lower income groups reported higher food insecurity perceptions and hoarding behaviors. Consuming COVID-19 information from websites was significantly associated with food insecurity perceptions, while information from social media was significantly associated with more food hoarding behaviors. Younger adults and lower income groups are vulnerable populations from the perspective of food insecurity and hoarding behavior in times of health disasters like pandemics. While social media can play a positively catalytic role during crises, excessive online information and misinformation can contribute negatively to public panic and feelings of insecurity. Implications for disaster preparedness and future research are discussed. The findings suggest that age is the main predictor of food insecurity and hoarding behavior, with younger adults more likely to be affected. They also suggest that people are turning to National Health Service (NHS) websites, which were deemed more trustworthy than social media, to avoid "news fatigue" and avoiding speculation. Suggestions for future research were made, specifically to examine people's social support during the pandemic to understand its potential link to stockpiling behavior or food insecurity concerns.

Estimating the long-term repeatability of food-hoarding behaviours in an avian predator.

Food-hoarding behaviour is widespread in the animal kingdom and enables predictable access to food resources in unpredictable environments. Within species, consistent variation among individuals in food-hoarding behaviours may indicate the existence of individual strategies, as it likely captures intrinsic differences in how individuals cope with risks (e.g. starvation, pilferage). Using 17 years of data, we estimated the long-term repeatability of 10 food-hoarding behaviours in a population of Eurasian pygmy owls (Glaucidium passerinum), a small avian predator subject to high temporal fluctuations in its main prey abundance. We found low repeatability in the proportion of shrews and the average prey mass stored for both sexes, while females were moderately repeatable in the mass and the number of prey items stored. These two pairs of behaviours were tightly correlated among individuals and might represent two different sets of individual strategies to buffer against starvation risks.

Panic buying? Food hoarding during the pandemic period with city lockdown

Food hoarding is prevalent during the COVID-19 pandemic. To investigate the mechanism of urban consumers' food hoarding behaviors, we categorize hoarding motives into rational and irrational ones. Using random online survey samples from three cities in China, we employ the multivariate probit model to investigate the rational and irrational motives on food hoarding behavior. Our results confirmed the existence of both rational and irrational food hoarding, and also found factors attributing to the different buying behaviors. The amount of food at hand and the expectation on the infection possibility of COVID-19 are two major factors affecting rational hoarding. Bad mood and herd psychology are factors contributing to panic buying. This study provides an empirical evidence to support intervention policies aiming at mitigating panic buying behavior.

The impact of online grocery shopping on stockpile behavior in Covid-19

PurposeThis research is to examine the impact of online channels on food stockpile behavior.Design/methodology/approachIn this study, we use bivariate probit models to empirically investigate the impact of online purchasing channels on Chinese urban consumer food hoarding behaviors with random survey samples.FindingsResults show that fresh food e-commerce channels are more likely to be associated with panic stockpile behaviors due to higher likelihood of supply shortages than offline channels with government assistance in logistic management. In contrast, community group buy, another format of e-commerce, appears superior in satisfying the consumer needs and easing the panic buying perception.Practical implicationsIt suggests that online channels may have diverse impacts on consumers' panic stockpiling behaviors during the extreme situations. Online channels need to develop efficient supply chains to be more resilient to extreme situations and the government shall recognize the increasing share of the online channels together with traditional offline channels when implementing supporting policies.Social implicationsWith ever increasing share of online channels, it is imperative in terms of policy implications to understand how would online channels affect hoarding behavior.Originality/valueWe are the first study in online shopping's impact on food stockpile during pandemics using a random sample. Although food stockpile behavior at times of emergency have been investigated in many literature, there are no empirical studies on the impact of online channels on stockpile behaviors under extreme situations. Unlike disasters that immediately impact every entity in supply chains covering producers, vendors, distribution centers and retailers, pandemics did not render supply chains affected immediately, but rather increase consumers' willingness to shop online to avoid virus. Thus, Covid-19 provides a natural experiment to investigate the online channels' impact on stockpile behavior.

Climate change and perishable food hoards of an avian predator: Is the freezer still working?

Changing climate can modify predator-prey interactions and induce declines or local extinctions of species due to reductions in food availability. Species hoarding perishable food for overwinter survival, like predators, are predicted to be particularly susceptible to increasing temperatures. We analysed the influence of autumn and winter weather, and abundance of main prey (voles), on the food-hoarding behaviour of a generalist predator, the Eurasian pygmy owl (Glaucidium passerinum), across 16years in Finland. Fewer freeze-thaw events in early autumn delayed the initiation of food hoarding. Pygmy owls consumed more hoarded food with more frequent freeze-thaw events and deeper snow cover in autumn and in winter, and lower precipitation in winter. In autumn, the rotting of food hoards increased with precipitation. Hoards already present in early autumn were much more likely to rot than the ones initiated in late autumn. Rotten food hoards were used more in years of low food abundance than in years of high food abundance. Having rotten food hoards in autumn resulted in a lower future recapture probability of female owls. These results indicate that pygmy owls might be partly able to adapt to climate change by delaying food hoarding, but changes in the snow cover, precipitation and frequency of freeze-thaw events might impair their foraging and ultimately decrease local overwinter survival. Long-term trends and future predictions, therefore, suggest that impacts of climate change on wintering food-hoarding species could be substantial, because their 'freezers' may no longer work properly. Altered usability and poorer quality of hoarded food may further modify the foraging needs of food-hoarding predators and thus their overall predation pressure on prey species. This raises concerns about the impacts of climate change on boreal food webs, in which ecological interactions have evolved under cold winter conditions.

Effects of food restriction on body mass, energy metabolism and thermogenesis in a tree shrew (Tupaia belangeri)

Abstract The metabolic switch hypothesis refers to an ability to adjust metabolic rate. It plays a key role in animals adapted to periods of food shortage, enabling them to “switch down” their resting metabolic rate and to survive and maintain their weight indefinitely on limited rations. The present study investigates the energy strategies of a small mammal in response to food shortages as a function of food restriction, metabolic rate and ambient temperature. We subjected tree shrews (Tupaia belangeri) to food restriction and measured body mass, survival rate, resting metabolic rate, non-shivering thermogenesis and cytochrome c oxidase activity of brown adipose tissue. Cold-exposed animals restricted to 80% of ad libitum food intake had significantly increased resting metabolic rate and non-shivering thermogenesis and decreased body mass and survival rates compared with those kept as control group on the same ood restriction level. Animals classified as having a high resting metabolic rate consumed 30.69% more food than those classified as having a low resting metabolic rate, but showed no differences in body mass or survival when restricted to 80% of ad libitum food intake. These results indicate that tree shrews, known for their relatively high metabolic rates, are sensitive to periods of food restriction, which supports the metabolic switch hypothesis. Our findings are also consistent with the prediction that small mammals with food hoarding behaviors, like tree shrews, may have a lower tolerance for food shortages than non-hoarding species.

Does caching strategy vary with microclimate in endangered Mt. Graham red squirrels?

Food hoarding is a common behavior used by a variety of animals to cope with periods of low food availability. At the retreating edge of species' distribution, the stressful environment and unfavourable climate conditions may impose severe costs on hoarding behavior. Since relict populations are hotspots for evolution and adaptation, and considering that food hoarding behavior has a strong evolutionary basis, we decided to evaluate the occurrence of behavioral variability in the amount of food cached by the endangered Mount Graham red squirrel (Tamiasciurus fremonti grahamensis). We tested the variation in cache size in response to microclimate, soil relief, vegetation, food availability and squirrel sex. The number of pits excavated by squirrels to cache cones was used as a proxy of cache size and was affected by mountain slope aspect and density of trees. More pits were excavated in the northeast facing slopes. The density of trees negatively affects the cache volume on southwest slopes, but not on northeast slopes. The sex of the resident squirrel also affects the number of pits in the squirrel midden, with males excavating 47% more pits than females. Males and females also presented different responses to the mountain slope aspect, with females excavating more pits on northeastern slopes than on southwestern slopes, whereas male cache size did not vary with the slope aspect. Finally, the squirrel's caching behavior did not vary in response to midden microclimate variation, a result with possible implications for the survival of the Mt Graham red squirrels, given the predicted temperature increases in the region due to climate change.

Scatterhoarders move pilfered seeds into their burrows

Although cache pilferage by food-hoarding animals has been well investigated, whether cache pilfering contributes to seed redistribution remains to be explored. In this study, we presented seeds exposed on the ground (including clumped and scattered seeds) and buried in artificial caches to Siberian chipmunks Tamias sibiricus and Korean wood mice Apodemus peninsulae exhibiting different food-hoarding behaviors, to investigate how seed distribution patterns affected seed fates and where pilfered seeds have gone in rodent-proof enclosures. Our results showed that both T. sibiricus and A. peninsulae handled more seeds exposed on the ground than buried in the caches. Scattered seeds were less likely to be eaten by rodents compared to clumped seeds and those buried in artificial caches. Exposed seeds in the enclosures were more likely to be scatterhoarded by T. sibiricus, while pilfered seeds from artificial caches were more likely to be larderhoarded than those easily accessible on the ground. Although buried seeds in artificial caches were less likely to be pilfered by rodents, both scatterhoarder (T. sibiricus) and larderhoarder (A. peninsulae) concealed pilfered seeds in their burrows. We also found that primary scatterhoarder T. sibiricus appeared to be effective pilferer, whereas A. peninsulae that primarily larderhoard seeds seemed to be poor pilferer, providing further evidence that the ability of food-hoarding animals to pilfer is correlated with how extensively they scatterhoard seeds. Our study highlights the importance of seed distribution patterns in affecting the hoarding behavior of scatterhoarding rodents, but not for larderhoarding rodents. Although cache pilferage by food-hoarding animals has been well investigated, whether cache pilfering contributes to seed redistribution remains to be well explored. Our study first showed that both scatterhoarders and larderhoarders concealed pilfered seeds in their burrows, challenging the previous assumption that cache pilferage will result in redistribution of dispersed seeds.

Improved spatial memory promotes scatter hoarding by Siberian chipmunks

Spatial memory can improve cache recovery in scatter-hoarding mammals; however, we lack knowledge about how spatial memory affects scatter-hoarding behavior. Captive-reared Siberian chipmunks (Tamias sibiricus) were orally dosed with docosahexaenoic acid (DHA) and uridine-5-monophosphate (UMP) for 6 weeks to test if improved spatial memory affects scatter hoarding. Oral administration of DHA and UMP significantly increased the relative size of hippocampi of the chipmunks and increased concentration of hippocampal DHA and eicosapentaenoic acid (EPA). Consequently, spatial ability of chipmunks in a Y-maze was significantly improved after administration of DHA and UMP. More importantly, chipmunks that received DHA and UMP scatter hoarded more seeds than control animals. This study shows that scatter hoarding in a mammal is associated with improvement in spatial memory, suggesting the potential role of spatial memory in determining food-hoarding behavior.

Picking the right cache: caching site selection for egg predators in the arctic

Food hoarding is often considered an adaptive behaviour to extend the period of availability of food resources. Finding the right caching site for storage and retrieval is of paramount importance, yet how caching sites are selected is poorly known. Here, we examine site selection for egg caching by a tundra predator, the arctic fox, Vulpes lagopus, which can overcome large seasonal prey variations by extensive caching. During the short arctic summer, colonial breeding birds like geese produce in a short time period many eggs, which provide a large quantity of predictable resources for consumption and caching. We predicted that foxes would select caching sites with specific characteristics, such as shallow permafrost, and tall hummocks (small mounds in tundra landscapes, which should facilitate caching and provide visual cues for subsequent retrieval). We sampled the main physical characteristics of 48 caches and paired random sites inside a Greater Snow Goose (Chen caerulescens) colony. Our study shows that foxes favour cache sites characterized by hummocks nearly twice as higher than at nearby random sites. The depth of the active layer of permafrost did not influence cache site selection. Foxes may select tundra features that possibly enhance caching efficiency and retrieval probability. Our study elucidates one aspect of food hoarding behaviour in extreme habitats characterized by strong variations of resource availability.

Paradoxical leanness in the imprinting-centre deletion mouse model for Prader-Willi syndrome.

Prader–Willi syndrome (PWS), a neurodevelopmental disorder caused by loss of paternal gene expression from 15q11–q13, is characterised by growth retardation, hyperphagia and obesity. However, as single gene mutation mouse models for this condition display an incomplete spectrum of the PWS phenotype, we have characterised the metabolic impairment in a mouse model for ‘full’ PWS, in which deletion of the imprinting centre (IC) abolishes paternal gene expression from the entire PWS cluster. We show that PWS-ICdel mice displayed postnatal growth retardation, with reduced body weight, hyperghrelinaemia and marked abdominal leanness; proportionate retroperitoneal, epididymal/omental and inguinal white adipose tissue (WAT) weights being reduced by 82%, 84% and 67%, respectively. PWS-ICdel mice also displayed a 48% reduction in proportionate interscapular brown adipose tissue (isBAT) weight with significant ‘beiging’ of abdominal WAT, and a 2°C increase in interscapular surface body temperature. Maintenance of PWS-ICdel mice under thermoneutral conditions (30°C) suppressed the thermogenic activity in PWS-ICdel males, but failed to elevate the abdominal WAT weight, possibly due to a normalisation of caloric intake. Interestingly, PWS-ICdel mice also showed exaggerated food hoarding behaviour with standard and high-fat diets, but despite becoming hyperphagic when switched to a high-fat diet, PWS-ICdel mice failed to gain weight. This evidence indicates that, unlike humans with PWS, loss of paternal gene expression from the PWS cluster in mice results in abdominal leanness. Although reduced subcutaneous insulation may lead to exaggerated heat loss and thermogenesis, abdominal leanness is likely to arise from a reduced lipid storage capacity rather than increased energy utilisation in BAT.

Visual landmark-directed scatter-hoarding of Siberian chipmunks Tamias sibiricus.

Spatial memory of cached food items plays an important role in cache recovery by scatter-hoarding animals. However, whether scatter-hoarding animals intentionally select cache sites with respect to visual landmarks in the environment and then rely on them to recover their cached seeds for later use has not been extensively explored. Furthermore, there is a lack of evidence on whether there are sex differences in visual landmark-based food-hoarding behaviors in small rodents even though male and female animals exhibit different spatial abilities. In the present study, we used a scatter-hoarding animal, the Siberian chipmunk, Tamias sibiricus to explore these questions in semi-natural enclosures. Our results showed that T. sibiricus preferred to establish caches in the shallow pits labeled with visual landmarks (branches of Pinus sylvestris, leaves of Athyrium brevifrons and PVC tubes). In addition, visual landmarks of P. sylvestris facilitated cache recovery by T. sibiricus. We also found significant sex differences in visual landmark-based food-hoarding strategies in Siberian chipmunks. Males, rather than females, chipmunks tended to establish their caches with respect to the visual landmarks. Our studies show that T. sibiricus rely on visual landmarks to establish and recover their caches, and that sex differences exist in visual landmark-based food hoarding in Siberian chipmunks.

Effects of thinning on scatter-hoarding by rodents in temperate forest.

Deforestation and thinning are human activities that can destabilize the forest ecological system and, consequently, impact significantly on habitat and behavior of forest-dwelling animals. This hypothesis was tested in Yugong in the Mount Taihangshan area by comparing the tracks of tagged seeds of Armeniaca sibirica. in sites of unthinned and thinned forests. Our results showed that: (i) the diversity of vegetation and rodents drastically reduced in sites with thinned forests, compared to unthinned sites; (ii) the amount of both removed and scatter-hoarded seeds significantly declined in sites with thinned forests, compared with the unthinned sites; (iii) there was no significant difference observed in the distance of seed dispersal between the thinned and unthinned areas; and (iv) the thinning did not show a significant change to the model of cache size. These results suggested that the thinning of forests negatively influenced the species richness and food-hoarding behavior of rodents. In addition, the results indicated that the weakened scattered-hoarding might be disadvantageous to seedling recruitment and forest restoration.

Short-term acute nitrogen deposition alters the interaction between Korean pine seeds and food hoarding rodents

Although the environmental impacts of nitrogen (N) deposition have been well studied, how nitrogen deposition affects the interaction between seeds and food hoarding animals has never been investigated. In this study, N was added twice as NH4NO3 to Korean pines Pinus koraiensis in order to explore the impacts of N deposition on seed traits and food-hoarding behaviors of small rodents in the northeastern deciduous forests in China. Pine cones were collected to measure seed traits and to test food-hoarding behaviors of small rodents both in the field and in semi-natural enclosures. Our results showed that short-term N addition significantly enhanced the ratio of seed meat to seed coats and the concentrations of crude protein. Moreover, seed volatile compounds were significantly altered by N addition, resulting in lower emission of α-pinene but higher emissions of D-limonene. Consequently, nitrogen addition increased seed scatter-hoarding by both Tamias sibiricus and Apodemus peninsulae in enclosures. Field experiments showed the consistent results that small rodents preferred to scatter-hoard seeds with N addition over the control. To our knowledge, this is the first study showing that nitrogen deposition shapes the interaction between seeds and food-hoarding animals through altering the physical and chemical traits of seeds.