Application of growth regulators like paclobutrazol and gibberellic acid is a suitable way to prevent excessive nitrogen consumption. Therefore, this 2-year research was conducted in Karaj, Iran. This research was laid out in a randomized complete block design with three replications in a split-plot arrangement in 2019–2021. Treatments included nitrogen at three levels: (60, 105 and 150 kg ha−1) which were placed in the main plots. The second factor was the foliar application of growth regulators at three levels: [gibberellic acid (100 ppm), paclobutrazol (200 ppm) and control]. The results showed that application of paclobutrazol and gibberellic acid raised super wheat yield and quality compared to control. The highest biological yield (27.67 ton ha−1), was observed in the interaction of nitrogen 150 kg ha−1 + gibberellic acid in the second year, which was the same with nitrogen 150 kg ha−1 + gibberellic acid in the first year and also interaction of nitrogen 150 kg ha−1 + paclobutrazol in the second year. The highest grain protein (14.69%) was seen in nitrogen 150 kg ha−1 + gibberellic acid in the second year and nitrogen 150 kg ha−1 + gibberellic acid in the first year. Growth regulators usage and increasing the nitrogen content (150 kg ha−1), improved the yield and grain quality of wheat. Therefore, the interaction of nitrogen 150 kg ha−1 + gibberellic acid and nitrogen 150 kg ha−1 +paclobutrazol, were the best treatments of this study, due to the creation of suitable environmental conditions for wheat, and these treatments can be suggested to the farmers.