Complex Folding Research Articles

Olfactory and gustatory chemical sensor systems in the African turquoise killifish: Insights from morphology.

Smell and taste are extensively studied in fish species as essential for finding food and selecting mates while avoiding toxic substances and predators. Depending on the evolutionary position and adaptation, a discrete variation in the morphology of these sense organs has been reported in numerous teleost species. Here, for the first time, we approach the phenotypic characterization of the olfactory epithelium and taste buds in the African turquoise killifish (Nothobranchius furzeri), a model organism known for its short lifespan and use in ageing research. Our observations indicate that the olfactory epithelium of N. furzeri is organized as a simple patch, lacking the complex folding into a rosette, with an average size of approximately 600µm in length, 300µm in width, and 70µm in thickness. Three main cytotypes, including olfactory receptor neurons (CalbindinD28K), supporting cells (β-tubulin IV), and basal cells (Ki67), were identified across the epithelium. Further, we determined the taste buds' distribution and quantification between anterior (skin, lips, oral cavity) and posterior (gills, pharynx, oesophagus) systems. We identified the key cytotypes by using immunohistochemical markers, i.e. CalbindinD28K, doublecortin, and neuropeptide Y (NPY) for gustatory receptor cells, glial fibrillary acidic protein (GFAP) for supporting cells, and Ki67, a marker of cellular proliferation for basal cells. Altogether, these results indicate that N. furzeri is a microsmatic species with unique taste and olfactory features and possesses a well-developed posterior taste system compared to the anterior. This study provides fundamental insights into the chemosensory biology of N. furzeri, facilitating future investigations into nutrient-sensing mechanisms and their roles in development, survival, and ageing.

Aerodynamic Analysis and Simulation for a Z-Shaped Folding Wing UAV

The potential of folding wing unmanned aerial vehicles (UAVs) in enhancing the adaptability of complex missions is substantial. Analyzing the aerodynamic characteristics of these UAVs is vital for optimizing the design of their folding wing configuration and airfoil shape. In this study, a model of a Z-shaped folding wing UAV was established with the objective of enhancing UAV maneuverability. An integrated vortex lattice method (VLM) was employed to analyze the aerodynamic characteristics of the Z-shaped folding wing and numerical simulations were utilized to validate the obtained results, which prove the effectiveness of the integrated VLM in studying aerodynamic characteristics at a high angle of attack of the Z-shaped folding wing UAV. This is a new way to reduce computation time while maintaining accuracy to calculate such complex folding structures in high fidelity. Furthermore, the Z-shaped folding wing configuration demonstrates enhanced maneuverability at high angles of attack, and a Simulink flight simulation model based on the aerodynamic coefficients of the Z-shaped folding wing verifies this property. This analysis can properly predict the post-stall characteristics for the Z-shaped folding wing UAV to ensure the safety of the vehicle during flight.

Computational design of soluble and functional membrane protein analogues

De novo design of complex protein folds using solely computational means remains a substantial challenge1. Here we use a robust deep learning pipeline to design complex folds and soluble analogues of integral membrane proteins. Unique membrane topologies, such as those from G-protein-coupled receptors2, are not found in the soluble proteome, and we demonstrate that their structural features can be recapitulated in solution. Biophysical analyses demonstrate the high thermal stability of the designs, and experimental structures show remarkable design accuracy. The soluble analogues were functionalized with native structural motifs, as a proof of concept for bringing membrane protein functions to the soluble proteome, potentially enabling new approaches in drug discovery. In summary, we have designed complex protein topologies and enriched them with functionalities from membrane proteins, with high experimental success rates, leading to a de facto expansion of the functional soluble fold space.

Evaluating Performance of Different RNA Secondary Structure Prediction Programs Using Self-cleaving Ribozymes.

Accurate identification of the correct, biologically relevant RNA structures is critical to understanding various aspects of RNA biology since proper folding represents the key to the functionality of all types of RNA molecules and plays pivotal roles in many essential biological processes. Thus, a plethora of approaches have been developed to predict, identify, or solve RNA structures based on various computational, molecular, genetic, chemical, or physicochemical strategies. Purely computational approaches hold distinct advantages over all other strategies in terms of the ease of implementation, time, speed, cost, and throughput, but they strongly underperform in terms of accuracy that significantly limits their broader application. Nonetheless, the advantages of these methods led to a steady development of multiple in silico RNA secondary structure prediction approaches including recent deep learning-based programs. Here, we compared the accuracy of predictions of biologically relevant secondary structures of dozens of self-cleaving ribozyme sequences using seven in silico RNA folding prediction tools with tasks of varying complexity. We found that while many programs performed well in relatively simple tasks, their performance varied significantly in more complex RNA folding problems. However, in general, a modern deep learning method outperformed the other programs in the complex tasks in predicting the RNA secondary structures, at least based on the specific class of sequences tested, suggesting that it may represent the future of RNA structure prediction algorithms.

Numerical modelling of different airbag folding patterns and their influence on occupant responses in frontal vehicle impact

This paper presented a procedure for airbag folding for the application in occupant safety studies and analysed the influence of different airbag folding patterns on the occupant severity in frontal impact. Airbags were folded in two patterns: zig-zag and top-roll, using two folding techniques: Initial Metric Method and Explicit Folding. The explicit folding was found to be more expensive in terms of preparation time. However, this approach provided more control over the whole folding process. The Initial Metric Method was more robust, however, it’s hard to apply for complex folding patterns. Deployments of airbags were validated against experimental data of pendulum tests. Those airbag models were applied to 2006 Ford F250 pickup truck. This vehicle was used for simulations of frontal vehicle impact where the 50th male Hybrid III crash test dummy was an occupant. Results of this simulation were compared with an actual test with a similar vehicle, under the same impact conditions. Results showed the head and chest accelerations were lower for top-roll folded airbag cases, however neck normal forces were higher compared to zig-zag folded airbag. The internal pressure in the early stage of deployment was 33% higher for the top-roll folded airbags.

The power of modern 3-D visualization of high-resolution terrain models in geologic mapping: Complex fold geometries revealed by 3-D mapping in the Panamint metamorphic complex, eastern California, USA

We use structure from motion–multiview stereo (SM) terrain models developed from ground-based images and images acquired from uncrewed aircraft (aka drones) as a base map for three-dimensional (3-D) mapping on the walls of a deep canyon in the Panamint Range of eastern California, USA. The ability to manipulate the 3-D model with views from arbitrary look directions and broad scale range revealed structures that were invisible to conventional two-dimensional (2-D) mapping because of both the scale of the structures and their exposure on vertical to near-vertical cliff faces. The analysis supports field evidence for four phases of ductile deformation, with only one of the younger phases documented on early geologic maps of the area. The oldest deformational event (D1) produced the main metamorphic fabric and pre-dates Late Cretaceous plutons. This deformation produced a 200–250-m-thick high-strain zone localized along marbles at the top of the Kingston Peak Formation and lower Noonday Formation. Geometric analysis from the model suggests strongly that large sheath folds at scales of 100–300 m are developed within these marbles. Large measured finite strains indicate displacement across this apparent shear zone of at least 4–5 km and displacements of tens of kilometers are allowable, yet the structure is invisible to conventional mapping because the high-strain zone is stratabound. The main fabric shows two clear overprints and a third that is likely an even younger deformation. D2 and D3 generated tight to close, recumbent folds and open to tight, upright folds, respectively, both folding the main foliation with localized development of crenulation cleavages axial planar to the folds. An additional overprint shows no clear cross-cutting relationship with D2 or D3 fabrics and could be a manifestation of either of those events, although the deformation is spatially limited to a narrow shear zone beneath a brittle, dextral-normal fault with the same kinematics as a mylonitic fabric in a Cretaceous granite in the footwall. This observation suggests an extensional, core complex–style deformation to produce this structure. We suggest that 3-D mapping has the potential to revolutionize geologic mapping studies, particularly where steep topography provides 3-D views that are virtually invisible on conventional 2-D maps. Previously bewildering geologic puzzles can be solved by the ability to visualize large cliff exposures from arbitrary angles and map the features in true 3-D at resolutions to the centimeter level. Although this study emphasized intermediate scales imaged by a drone, our methods here are easily extended to larger scales using a crewed aircraft for imaging. We suggest these methods should be used routinely in frontier areas with steep terrain where aviation is already in use for access, but the methods can be employed anywhere steep terrain “hides” major rock exposures on conventional 2-D maps.

Lithologic Relationship and Structural Features of Crystalline Rocks in Ekiti, Southwestern Nigeria: A Geological Report on the Basement Complex

This paper investigates and report field relationship and structural features of the basement rocks in Ekiti, southwestern Nigeria. Systematic geological mapping reveals that Ekiti is underlain by migmatite-gneiss, quartz schist, quartzite, granite, and charnockite. These lithologies were intruded by series of aplite, dolerite, and pegmatite dykes. Structural deformations attributable to polycyclic orogenic activities manifested in all the basement rocks. Migmatite and its gneissic subunits form the country rock and exhibits complex folds, abundant quartz veins and haphazardly emplaced dykes. Foliation and lineament are oriented N-S, strike values range between N5oE -N18oE with westerly dip of 72º to 84º. Quartzite and quartz schist exhibits distinctive joint and fracture system which runs parallel to one another while minor ones are oblique. The polymetamorphic terrain has older tectonic fabrics massively overprinted by younger ones. The gneiss units are porphyroblastic to granoblastic, xenoliths of varying sizes are embedded within the granite bodies indicating the magmatic liquid was forcefully injected into the country rock. The presence of parallel fractures in fine-grained granite indicate Ekiti area is a shear zone. The occurrence of dykes of heterogenous lithologies on single granite outcrop suggests pluton assemblage occur as discrete pulses during the intrusive phases. Contact relationship indicate age decreases from migmatite to granite-charnockite to microgranite while dykes are the youngest. Deformation, metamorphism, and intrusive phases are the dominant geodynamic features of Ekiti area while the regional and local changes in rocks resulted from environmental constraints of pressure, temperature, and fluid activity. The plutonic activities which accompanied orogenic events resulted in deformation which is genetically related to evolution and geodynamic setting of Ekiti area.

Investigating subduction orogeny dynamics of Manabhum high in the Upper Assam foreland basin, NE India by geophysical data-based modelling

The Manabhum structural high, situated on the southern bank of the Brahmaputra River in the foreland part of the Upper Assam basin, is characterised by an elongated NNW-SSE direction of the fold axis. This study utilises seismic and satellite gravity data to model subsurface geological cross-sections across the Manabhum structure, providing insights into the presence of high-density material at the basement level, supported by the distribution of ophiolites at varying depths. The Manabhum, a compressional structure controlled by the complex folding involved in the Mishmi Hills and Naga Schuppen belt, subjected to compression at different stages, presents an excellent opportunity to understand subduction orogeny involving the collision between the Indian plate and the Tethys oceanic lithosphere subducting beneath the Myanmar continental lithosphere.

DNA-Mediated Peptide Assembly into Protein Mimics.

The design of new protein structures is challenging due to their vast sequence space and the complexity of protein folding. Here, we report a new modular DNA-templated strategy to construct protein mimics. We achieve the spatial control of multiple peptide units by conjugation with DNA and hybridization to a branched DNA trimer template followed by covalent stapling of the preorganized peptides into a single unit. A library of protein mimics with different lengths, sequences, and heptad registers has been efficiently constructed. DNA-templated protein mimics show an α-helix or coiled-coil motif formation even when they are constructed from weakly interacting peptide units. Their attached DNA handles can be used to exert dynamic control over the protein mimics' secondary and tertiary structures. This modular strategy will facilitate the development of DNA-encoded protein libraries for the rapid discovery of new therapeutics, enzymes, and antibody mimics.

Middle Miocene renewed subduction of arabian-eurasian plates: Implications for convergent tectonic mechanisms, tectonically-induced fluid overpressures, and sediment recycling dynamics

The complex interplay between thrust wedge deformation, tectonically-induced fluid overpressures, and the complex phases of wedge-top sedimentary depositional systems in active plate convergent margins presents a formidable challenge. The offshore Makran accretionary wedge (MAW) is the world's largest, formed by the convergence of Arabian plate underneath Eurasian plate in the Early Cretaceous, followed by Middle Miocene renewed subduction. In this research, geological and geophysical constraints derived from seismic and borehole stratigraphy, seismic attributes, depth structural maps, isopach maps and 3D structural geological models reveal that Middle Miocene renewed subduction controls thrust wedge deformation. Additionally, it influences deep fluid overpressure and the deformational pattern of wedge-top sedimentary depositional systems. The seismic data analysis suggested that the basement pre-kinematic Himalayan turbidities depositional system (HTDs) have been tectonically incorporated into the accretionary wedge by Middle Miocene renewed subduction, which gave rise to complex folding and N-dipping imbricate thrusting features. These N-dipping imbricate thrust faults initiated sediment underplating and served as major structural pathways for deep fluid overpressure migration, resulting in the formation of fluid escape pipes. The fluid escape pipes exhibit conical and bifurcating geometries with inclination angles of approximately 73°, 90°, and 100°, signifying the upward migration of deep overpressured fluids. Furthermore, the Middle Miocene to Pliocene syn-kinematic piggyback and growth depositional system (PGDs) show progressive thickening (Max ∼3600 m) towards onshore MAW. It lies unconformable above the pre-kinematic HTDs and carries valuable sedimentation records, growth stratal patterns, onlap terminations, and truncations against growing structures, revealed the timing and spatial deformation of thrust faults in 3D space. In contrast, the Pleistocene to Recent post-kinematic progradational depositional system (PDs) exhibits clinoforms marked by top-lapping, on-lapping, and down-lapping reflection, which are primarily controlled by sediment recycling dynamics and sea level change since Pleistocene. It shows progressive thickening (Max ∼1800m) towards the Makran subduction zone and lies unconformably above the syn-kinematic PGDs. Abridging the analyses, the tectonic reconstruction and deformation mechanisms model shows four phases, e.g. (1) initiation of subduction and accretionary wedge formation in Eocene, (2) N-dipping imbricate thrust faults and deep fluid overpressure triggered by Middle Miocene renewed subduction, (3) development of piggyback basins and growth stratal geometries in Middle Miocene to Pliocene, (4) thrust deformation ceases and development of progradational depositional system.

Diversity Analysis of Catechol 2, 3-dioxygenase in POPs Metabolizing Bacteria using In-silico Approach

The persistent nature of persistent organic pollutants (POPs), lipophilicity, and volatility has resulted in their high concentration, not only in environmental resources but also in living organisms. Their complete removal is possible only by mineralization using enzyme-based strategies. Catechol 2, 3-dioxygenase is reportedly involved in the degradation of a wide variety of POPs. This study was designed to determine the diversity of this enzyme among highly efficient bioremediating bacteria. Seven bacteria belonging to genera acinetobacter, pseudomonas, burkholderia, stutzerimonas, and paraburkholderia were targeted. Sequences of the enzyme were retrieved from Uniprot database and analyzed via ProtParam, CELLU, and SOPMA tools and AlphaFold database. The enzyme was found to be cytoplasmic. The physicochemical properties of the enzyme were recorded as pI 4.75 – 5.50, aliphatic index (73.41 – 88.55), instability index (24.98 – 43.37), and GRAVY (-0.209 – 0.511). The secondary structure attributes were recorded to be α-helix (30.13 – 37.30), extended strand (18.27 – 21.54), β-turn (5.14 – 6.95), and random coil (38.33 – 42.95). All the proteins showed complex folding except in Pseudomonas sp strain EST1001. These properties may be exploited during the selection, purification, manipulation, and cloning of the enzyme for efficient bioremediation.

Dynamic physical-geological model of complex folding according to paleomagnetic data

The purpose of the study is to evaluate the possibility of using the mathematical apparatus of spatial rotations to solve the determination issues of the nature and age of natural remanent magnetization vectors under complex tectonic dislocations of rocks manifested in the areas of modern folded structures of platform framing. Dynamic physical-geological models are shown to be useful when forming complex tectonic-magmatic systems. Mathematical (computer) modeling in comparison with other methods of studying geological processes features higher accuracy, cost-effectiveness and unambiguity of data interpretation in achieving the set goal. On the basis of dynamic physical-geological model of complex folding, an algorithm is developed and the results of mathematical modeling of natural remanent magnetization vectors are given for solving direct and inverse problems on correct application of the fold test under complex rock deformations. The dynamic physical-geological model of complex folded structure formation shows that the characteristic natural remanent magnetization vectors identified in laboratory experiments on demagnetization can be used to determine their age relative to the folding stages, and, depending on this, fully or partially restore the number, sequence and direction of tectonic dislocations. This will enable us to solve the mineralogenic and geodynamic problems of folded region development more effectively on the basis of paleomagnetic data.

Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coli

With increased accessibility and tissue penetration, smaller antibody formats such as antibody fragments (Fab) and single chain variable fragments (scFv) show potential as effective and low-cost choices to full-length antibodies. These formats derived from the modular architecture of antibodies could prove to be game changers for certain therapeutic and diagnostic applications. Microbial hosts have shown tremendous promise as production hosts for antibody fragment formats. However, low target protein yields coupled with the complexity of protein folding result in production limitations. Here, we report an alternative antibody fragment format ‘FabH3’ designed to overcome some key bottlenecks associated with the folding and production of Fabs. The FabH3 molecule is based on the Fab format with the constant domains replaced by engineered immunoglobulin G1 (IgG1) CH3 domains capable of heterodimerization based on the electrostatic steering approach. We show that this alternative antibody fragment format can be efficiently produced in the cytoplasm of E. coli using the catalyzed disulfide-bond formation system (CyDisCo) in a natively folded state with higher soluble yields than its Fab counterpart and a comparable binding affinity against the target antigen.

Production of recombinant human proliferating cellular nuclear antigen (PCNA) for structural and biophysical characterization

Human proliferating cell nuclear antigen (hPCNA) is a DNA replication processivity factor, which acts as a docking platform, allowing proteins to have access to the replication fork and increasing the affinity of DNA interacting proteins, making it critical for cell survival. The trimer forms a ring-shaped oligomer allowing DNA to pass through the middle and interacting proteins to dock on the outside of the ring. Without this structural formation, there is a loss of DNA replication and repair in the cell. Due to the location of subunit-subunit termini, the addition of a purification tag can hamper crystallography and biophysical experiments, as the trimer complex folding can be impeded. To avoid these complications, a tag-less, step-wise purification was implemented, which resulted in 17.6 mg from 2 L culture of pure hPCNA with a 260 nm/280 nm value of 0.43. The produced crystal structure reveals a correctly formed oligomer. The clear depletion of the tracer binding and probe protein interaction in a fluorescence polarisation competition-based assay demonstrates the purification method produces a protein structure with a functional binding site. This purification method presents a reliable and simple method for producing hPCNA for biophysical characterisation.

Replacement of the native cis prolines by alanine leads to simplification of the complex folding mechanism of a small globular protein

The folding mechanism of MNEI, a single-chain variant of naturally occurring double-chain monellin, is complex, with multiple parallel refolding channels. To determine whether its folding energy landscape could be simplified, the two native cis-prolines, Pro41 and Pro93, were mutated, singly and together, to Ala. The stability of P93A was the same as that of the wild-type protein, pWT; however, P41A and P41AP93A were destabilized by ∼0.9 kcal mol−1. The effects of the mutations on the very fast, fast, slow, and very slow phases of folding were studied. They showed that heterogeneity in the unfolded state arises due to cis to trans isomerization of the Gly92-Pro93 peptide bond. The Pro41 to Ala mutation abolished the very slow phase of folding, whereas surprisingly, the Pro93 to Ala mutation abolished the very fast phase of folding. Double-jump, interrupted folding experiments indicated that two sequential trans to cis proline isomerization steps, of the Gly92-Pro93 peptide bond followed by the Arg40-Pro41 peptide bond, lead to the formation of the native state. They also revealed the accumulation of a late native-like intermediate, N∗, which differs from the native state in the isomeric status of the Arg40-Pro41 bond, as well as in a few tertiary contacts as monitored by near-UV CD measurements. The Pro to Ala mutations not only eliminated the cis to trans Pro isomerization reaction in the unfolded state, but also the two trans to cis Pro isomerization reactions during folding. By doing so, and by differentially affecting the relative stabilities of folding intermediates, the mutations resulted in a simplification of the folding mechanism. The two Pro to Ala mutations together accelerate folding to such an extent that the native state forms more than 1000-fold faster than in the case of pWT.

Praziquantel inhibits Caenorhabditis elegans development and species-wide differences might be cct-8-dependent.

Anthelmintic drugs are used to treat parasitic roundworm and flatworm infections in humans and other animals. Caenorhabditis elegans is an established model to investigate anthelmintics used to treat roundworms. In this study, we use C. elegans to examine the mode of action and the mechanisms of resistance against the flatworm anthelmintic drug praziquantel (PZQ), used to treat trematode and cestode infections. We found that PZQ inhibited development and that this developmental delay varies by genetic background. Interestingly, both enantiomers of PZQ are equally effective against C. elegans, but the right-handed PZQ (R-PZQ) is most effective against schistosome infections. We conducted a genome-wide association mapping with 74 wild C. elegans strains to identify a region on chromosome IV that is correlated with differential PZQ susceptibility. Five candidate genes in this region: cct-8, znf-782, Y104H12D.4, Y104H12D.2, and cox-18, might underlie this variation. The gene cct-8, a subunit of the protein folding complex TRiC, has variation that causes a putative protein coding change (G226V), which is correlated with reduced developmental delay. Gene expression analysis suggests that this variant correlates with slightly increased expression of both cct-8 and hsp-70. Acute exposure to PZQ caused increased expression of hsp-70, indicating that altered TRiC function might be involved in PZQ responses. To test if this variant affects development upon exposure to PZQ, we used CRISPR-Cas9 genome editing to introduce the V226 allele into the N2 genetic background (G226) and the G226 allele into the JU775 genetic background (V226). These experiments revealed that this variant was not sufficient to explain the effects of PZQ on development. Nevertheless, this study shows that C. elegans can be used to study PZQ mode of action and resistance mechanisms. Additionally, we show that the TRiC complex requires further evaluation for PZQ responses in C. elegans.

Linking folding dynamics and function of SAM/SAH riboswitches at the single molecule level.

Riboswitches are regulatory elements found in bacterial mRNAs that control downstream gene expression through ligand-induced conformational changes. Here, we used single-molecule FRET to map the conformational landscape of the translational SAM/SAH riboswitch and probe how co-transcriptional ligand-induced conformational changes affect its translation regulation function. Riboswitch folding is highly heterogeneous, suggesting a rugged conformational landscape that allows for sampling of the ligand-bound conformation even in the absence of ligand. The addition of ligand shifts the landscape, favoring the ligand-bound conformation. Mutation studies identified a key structural element, the pseudoknot helix, that is crucial for determining ligand-free conformations and their ligand responsiveness. We also investigated ribosomal binding site accessibility under two scenarios: pre-folding and co-transcriptional folding. The regulatory function of the SAM/SAH riboswitch involves kinetically favoring ligand binding, but co-transcriptional folding reduces this preference with a less compact initial conformation that exposes the Shine-Dalgarno sequence and takes min to redistribute to more compact conformations of the pre-folded riboswitch. Such slow equilibration decreases the effective ligand affinity. Overall, our study provides a deeper understanding of the complex folding process and how the riboswitch adapts its folding pattern in response to ligand, modulates ribosome accessibilityand the role of co-transcriptional folding in these processes.

Membrane anatomy-based splenic hilar lymph node dissection for gastric cancer

There is a consensus that selectively perform splenic lymph node dissection is necessary for high-risk patients with proximal gastric cancer to achieve radical treatment. However, there are still some outstanding issues that need to be solved during the practice of splenic lymph node dissection. These include poorly defined boundaries, technical difficulties, and blurred boundaries in No. 10 and No. 11 lymph nodes, etc. Membrane anatomy has achieved successful applications in the field of gastric and colorectal surgery in recent years. The study of membrane anatomy in the splenic hilum region is controversial due to the special location of the splenic hilum, which involves multiple organs and affiliated mesentery undergoing complex rotation, folding, and fusion during embryonic development. In this manuscript, we summarize the following points based on existing research and personal experience regarding membrane anatomy. 1. There is a membrane anatomical structure that can be used for lymph node dissection in the splenic hilum region. 2. The membrane structure in the splenic hilum region can be divided into two layers: the superficial layer is composed of the dorsal mesogastrium, and the deep layer is composed of Gerota fascia, the tail of the pancreas, and the mesentery of the transverse colon (from head to tail). 3. There is a loose space between the two layers that can be used for separation during surgery. The resection of the dorsal mesogastrium belongs to D2 dissection. The No. 10 lymph node in the deeper layer belongs to the duodenal mesentery, and the resection of the No.10 lymph node exceeds D2 dissection. The complete excision of the gastric dorsal mesentery is consistent with the D2+CME surgical mode proposed by Gong Jianping's group.

Temporal fingerprints of cortical gyrification in marmosets and humans.

Recent neuroimaging studies in humans have reported distinct temporal dynamics of gyri and sulci, which may be associated with putative functions of cortical gyrification. However, the complex folding patterns of the human cortex make it difficult to explain temporal patterns of gyrification. In this study, we used the common marmoset as a simplified model to examine the temporal characteristics and compare them with the complex gyrification of humans. Using a brain-inspired deep neural network, we obtained reliable temporal-frequency fingerprints of gyri and sulci from the awake rs-fMRI data of marmosets and humans. Notably, the temporal fingerprints of one region successfully classified the gyrus/sulcus of another region in both marmosets and humans. Additionally, the temporal-frequency fingerprints were remarkably similar in both species. We then analyzed the resulting fingerprints in several domains and adopted the Wavelet Transform Coherence approach to characterize the gyro-sulcal coupling patterns. In both humans and marmosets, sulci exhibited higher frequency bands than gyri, and the two were temporally coupled within the same range of phase angles. This study supports the notion that gyri and sulci possess unique and evolutionarily conserved features that are consistent across functional areas, and advances our understanding of the functional role of cortical gyrification.

Predicting the expansion of the lower pole of the breast following smooth breast implant augmentation: A novel shear wave elastography study

This study aimed to educate and demonstrate how the use of shear wave elastography (SWE) can be used to determine the elasticity of patient tissues preoperatively, which can then be used to predict the level of lower pole expansion postoperatively, following breast augmentation surgery. This study evaluated 60 breasts in 30 patients that were divided in 3 equal groups (n=20) according to their predefined elastography criteria measured via SWE (loose, moderate, and tight tissue elasticity). All measurements were taken under maximum stretch between the inferior border of the nipple alveolar complex (NAC) and inframammary fold (IMF) using a measuring tape in millimetres (mm). The follow-up appointments for routine assessments and measurements were done at 3, 6, 12, 18, and 24 months. The study engaged 38 patients over 4 years, but only 10 patients in each group attended all the appointments. Statistical analysis showed the elastic skin types (loose, moderate, and tight) had significantly different rates of lower pole expansion, and the rate of expansion increased significantly after 6 months postoperatively, whereas prior to 6 months, the rates were comparable (p<0.05). The results showed that increasingly elastic skin types have a greater rate of lower pole expansion. This is important for the operating surgeon to be aware of as looser skin types will be more prone to lower pole expansion, and thus, a higher surgical IMF suture may be advised to manage patient expectations. This study can be used as a guideline for surgeons, which will allow for a more predictable surgical planning system that will ultimately lead to fewer revisions and risks for patients worldwide.