Fluorescent Probe For Hydrazine Research Articles

A Fluorescent Probe for Hydrazine Based on 4-hydroxycoumarin with High Selectivity and Sensitivity

Background: Therefore, the development of reliable analytical techniques with high selectivity and sensitivity to detect hydrazine is required for the protection of human health and safety. Objectives: Traditional methods for detecting N2H4 are frequently time-consuming, less accurate, and unsuitable for the analysis of living systems. Numerous fluorescent probes for hydrazine have been produced and gained some valuable results recently. The creation of a simple fluorescent probe for hydrazine detection is the goal of this project Method: In this study, 300 µL of probe 3-methyl-2-oxo-2H-chromen-7-yl propionate (MOCP) was mixed with an equivalent amount of the solution of each analyte to obtain the measurement solution. Following a 10-minute room temperature incubation period, the fluorescence spectra of the resultant solution were recorded. Results: The fluorescence intensity of the probe was noticeably enhanced when N2H4 was added to the probe, but almost no fluorescence enhancement was observed when other competitive ions were added. Conclusion: A hydrazine fluorescent probe based on 4-hydroxycoumarin fluorophore was developed. The probe MOCP displayed high sensitivity and selectivity for hydrazine, with a color change from colourless to blue for detection by the naked eye. Moreover, it demonstrated a low detection limit of 20 nM and a fast reaction time of 30 s.

Sensing for hydrazine of a pyrene chalcone derivative with acryloyl terminal group

Hydrazine, as a toxic substance, seriously endangers human health and the environment. Based on the excellent luminescent properties and low biological toxicity of pyrene derivatives, combing with chalcone derivatives easily attacked by nucleophilic group, a pyrene derivative PCA decorated by acryloyl terminal group as fluorescent probe for hydrazine was developed. The compound shows fluorescent peak red shift and intensity enhancement with increasing solvent polarity from hexane (459 nm) to methanol (561 nm). Based on strong fluorescence emission in methanol, methanol-HEPES mixed solution was used as the solvent in the spectral recognition experiments. The probe exhibits fluorescent change from yellow fluorescence (576 nm) to blue fluorescence (393 nm) with 800-fold ratiometric fluorescence enhancement (I393nm/I576nm) after the reaction with hydrazine. The probe can recognize hydrazine in fast response rate with kinetic constant calculated being 2.7 × 10-3 s−1 and 15 min as response time. The probe also can monitor hydrazine in real water samples and various soils.

A stable hydrazine click fluorescent probe based on photo switch

One novel aggregation induced emission (AIE) fluorescent probe for hydrazine (N2H4) (T) was synthesized. This is an optically controlled process. The probe is very stable before light, which is different from many probes with ester bonds that are easily hydrolyzed during storage. Only under ultraviolet (UV) light irradiation, T responded to N2H4 rapidly in aqueous solution along with the fluorescence change from yellow to blue. The detection limit is as low as 0.25 μM, and it works well at a wide pH range (3–10) in aqueous solutions. Bio-imaging of N2H4 in living cell demonstrated a favorable biocompatibility. Moreover, the optically controlled mechanism was exploited. The result indicated that the Schiff base structure of T would be twisted under UV light irradiation, which loosened the original aggregation state. Consequently, T became more accessible to N2H4. That is, aggregation state of T is inactive, only in the presence of UV light the reactivity could be activated. Interestingly, the torsion of this compound can be restored by visible light irradiation or heating. This is the first report about optically controlled AIE fluorescent probe.

A Fluorescent Probe for Hydrazine Based on Synergistic Effect and Its Utilization in Cell Imaging

A Fluorescent Probe for Hydrazine Based on Synergistic Effect and Its Utilization in Cell Imaging

A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells

A Flavone-Based Fluorescent Probe for Hydrazine and Its Bioimaging in Live Cells

A Fluorescein-Based Colorimetric and Fluorescent Probe for Hydrazine and its Bioimaging in Live Cells.

A turn-on fluorescent probe (FN) for detection of hydrazine has been developed. Probe FN exhibited high selectivity and excellent sensitivity towards hydrazine with a detection limit as low as 4.6×10-10M. Probe FN selectively reacts with hydrazine (N2H4) in a physiological environment, leading to an off-on fluorescence response along with the color change from colorless to yellow, allowing colorimetric detection of hydrazine by the naked eye. Furthermore, probe FN was successfully applied for visualizing hydrazine in living cells.

A new colorimetric and far-red fluorescent probe for hydrazine with a large red-shifted absorption spectrum.

Recently, growing attention has been paid to the detection of hydrazine (NH2 NH2 ) because of its important roles in industrial chemical and high toxicity to human beings. Herein, we have constructed a new colorimetric and far-red fluorescent probe containing a receptor of 4-bromobutanoate to selectively detect hydrazine. The probe could detect hydrazine quantitatively in the range of 40-500μM with the detection limit of 2.9μM. In addition, the probe could monitor hydrazine by the ratiometric method with a large (185nm) red-shifted absorption spectrum, and the color changes from yellow to blue make it as a 'naked-eye' indicator for hydrazine. Consequently, our proposed probe would be of great benefit for monitoring hydrazine in aqueous solution.

A fluorescent probe for hydrazine and its in vivo applications

In aqueous solution, probe 1 selectively reacts with hydrazine (N2H4), leading to a 30.5-fold turn-on fluorescence response at 560 nm. Probe 1 can detect hydrazine in both solution and gas state by color changes from readily prepared strips. Furthermore, it was found that probe 1 is able to detect hydrazine in Hela cells.