Fluorescent Polystyrene Beads Research Articles

Smartphone-based optical sectioning (SOS) microscopy with a telecentric design for fluorescence imaging.

We propose a smartphone-based optical sectioning (SOS) microscope based on the HiLo technique, with a single smartphone replacing a high-cost illumination source and a camera sensor. We built our SOS with off-the-shelf optical, mechanical cage systems with 3D-printed adapters to seamlessly integrate the smartphone with the SOS main body. The liquid light guide can be integrated with the adapter, guiding the smartphone's LED light to the digital mirror device (DMD) with neglectable loss. We used an electrically tuneable lens (ETL) instead of a mechanical translation stage to realise low-cost axial scanning. The ETL was conjugated to the objective lens's back pupil plane (BPP) to construct a telecentric design by a 4f configuration to maintain the lateral magnification for different axial positions. SOS has a 571.5µm telecentric scanning range and an 11.7µm axial resolution. The broadband smartphone LED torch can effectively excite fluorescent polystyrene (PS) beads. We successfully used SOS for high-contrast fluorescent PS beads imaging with different wavelengths and optical sectioning imaging of multilayer fluorescent PS beads. To our knowledge, the proposed SOS is the first smartphone-based HiLo optical sectioning microscopy (£1965), which can save around £7035 compared with a traditional HiLo system (£9000). It is a powerful tool for biomedical research in resource-limited areas.

Imperfect refractive index matching in scanning laser optical tomography and a method for digital correction.

Scanning laser optical tomography (SLOT) is a volumetric multi-modal imaging technique that is comparable to optical projection tomography and computer tomography. Image quality is crucially dependent on matching the refractive indexes (RIs) of the sample and surrounding medium, but RI matching often requires some effort and is never perfect. Reducing the burden of RI matching between the immersion medium and sample in biomedical imaging is a challenging and interesting task. We aim at implementing a post processing strategy for correcting SLOT measurements that have errors caused by RI mismatch. To better understand the problems with poorly matched Ris, simulated SLOT measurements with imperfect RI matching of the sample and medium are performed and presented here. A method to correct distorted measurements was developed and is presented and evaluated. This method is then applied to a sample containing fluorescent polystyrene beads and a sample made of olydimethylsiloxane with embedded fluorescent nanoparticles. From the simulations, it is evident that measurements with an RI mismatch larger than 0.02 and no correction yield considerably worse results compared to perfectly matched measurements. RI mismatches larger than 0.05 make it almost impossible to resolve finer details and structures. By contrast, the simulations imply that a measurement with an RI mismatch of up to 0.1 can still yield reasonable results if the presented correction method is applied. The experiments validate the simulated results for an RI mismatch of about 0.09. The method significantly improves the SLOT image quality for samples with imperfectly matched Ris. Although the absolutely best imaging quality will be achieved with perfect RI matching, these results pave the way for imaging in SLOT with RI mismatches while maintaining high image quality.

Polystyrene Plastic Particles Result in Adverse Outcomes for Hyalella azteca When Exposed at Elevated Temperatures

Micro- and nano-plastics are pervasive pollutants in global ecosystems, yet their interactions with aquatic wildlife and abiotic factors are poorly understood. These particles are recognized to cause subtle detrimental effects, underscoring the necessity for sensitive endpoints in ecotoxicological exposure studies. We investigated the effects of particle uptake, size, and temperature on Hyalella azteca. Organisms were exposed to blue fluorescent polystyrene beads (500 nm and 1000 nm in diameter) at 0.43 mg/L for 96 h at temperatures mirroring climate predictions (21 °C, 24 °C, 27 °C). Besides survival and growth, particle uptake, visualized via confocal microscopy, and swimming behavior were analyzed. Mortality rates increased at 27 °C, and particle presence and temperature affected organism growth. Particle treatments influenced various behaviors (thigmotaxis, cruising, movement, acceleration, meander, zone alternation, and turn angle), with hypoactivity observed with 1000 nm particles and hypo- as well as hyper-activity responses with 500 nm particles. Particle uptake quantities were variable and increased with temperature in 500 nm treatments, but no migration beyond the gut was observed. Particle size correlated with uptake, and relationships with behavior were evident. Elevated temperatures exacerbated particle effects, highlighting the urgency of addressing plastic pollution in light of climate change for aquatic organism welfare and ecosystem health.

Size dependent uptake and trophic transfer of polystyrene microplastics in unicellular freshwater eukaryotes

Microplastics (MP) have become a well-known and widely investigated environmental pollutant. Despite the huge amount of new studies investigating the potential threat posed by MP, the possible uptake and trophic transfer in lower trophic levels of freshwater ecosystems remains understudied. This study aims to investigate the internalization and potential trophic transfer of fluorescent polystyrene (PS) beads (0.5 μm, 3.6 × 108 particles/mL; 6 μm, 2.1 × 105 particles/mL) and fragments (<30 μm, 5 × 103 particles/mL) in three unicellular eukaryotes. This study focuses on the size-dependent uptake of MP by two freshwater Ciliophora, Tetrahymena pyriformis, Paramecium caudatum and one Amoebozoa, Amoeba proteus, serving also as predator for experiments on potential trophic transfer. Size-dependent uptake of MP in all three unicellular eukaryotes was shown. P. caudatum is able to take up MP fragments up to 27.7 μm, while T. pyriformis ingests particles up to 10 μm. In A. proteus, small MP (PS0.5μm and PS6μm) were taken up via pinocytosis and were detected in the cytoplasm for up to 14 days after exposure. Large PS-MP (PS<30μm) were detected in A. proteus only after predation on MP-fed Ciliophora. These results indicate that A. proteus ingests larger MP via predation on Ciliophora (PS<30μm), which would not be taken up otherwise. This study shows trophic transfer of MP at the base of the aquatic food web and serves as basis to study the impact of MP in freshwater ecosystems.

Optofluidic Flow Cytometer with In-Plane Spherical Mirror for Signal Enhancement.

Statistical analysis of the properties of single microparticles, such as cells, bacteria or plastic slivers, has attracted increasing interest in recent years. In this regard, field flow cytometry is considered the gold standard technique, but commercially available instruments are bulky, expensive, and not suitable for use in point-of-care (PoC) testing. Microfluidic flow cytometers, on the other hand, are small, cheap and can be used for on-site analyses. However, in order to detect small particles, they require complex geometries and the aid of external optical components. To overcome these limitations, here, we present an opto-fluidic flow cytometer with an integrated 3D in-plane spherical mirror for enhanced optical signal collection. As a result, the signal-to-noise ratio is increased by a factor of six, enabling the detection of particle sizes down to 1.5 µm. The proposed optofluidic detection scheme enables the simultaneous collection of particle fluorescence and scattering using a single optical fiber, which is crucial to easily distinguishing particle populations with different optical properties. The devices have been fully characterized using fluorescent polystyrene beads of different sizes. As a proof of concept for potential real-world applications, signals from fluorescent HEK cells and Escherichia coli bacteria were analyzed.

NIR-II fluorescent Ag2Se polystyrene beads in a lateral flow immunoassay to detect biomarkers for breast cancer.

Fluorescent lateral flow immunoassay (LFA), one tool in point of care testing (POCT) systemsfor breast cancer, has attracted attention because it is quick, simple, and convenient. However, samples and the constituent material exhibit autofluorescence in the visible region, which is a very large obstacle in the development of fluorescent LFAs. The autofluorescence of biological samples is scarcely found in the second near-infrared (NIR-II) range and samples scatter and absorb less NIR-II light than visible light. Here, we report an NIR-II QD-LFA platform using the NIR-II fluorescent Ag2Se quantum dots (QDs) with 1020nm emission encapsulated into polystyrene beads as fluorescent probes. The NIR-II LFA platform was established to detect breast cancer tumour markers (CEA and CA153) within 15min with a low limit of detection (CEA: 0.768ngmL-1, CA153: 1.192 U mL-1), high recoveries (93.7% ~ 108.8%), and relative standard deviations (RSDs) of less than 10%. This study demonstrated the potential of NIR-II Ag2Se polystyrene beads as a fluorescent probe in LFA for rapid and accurate identification of biomarkers. They are suited for use in professional situations.

Oxidative stress and histopathological effects by microplastic beads, in the crayfish Procambarus clarkii, and fiddler crab Leptuca pugilator

The present study was aimed at evaluating the in vivo effects of microplastics (MP), in terms of oxidative stress and histopathological effects, in two crustacean species: Procambarus clarkii and Leptuca pugilator. In addition, MP accumulation in the hepatopancreas (HP) of both species was also determined. Adults of both crayfish and crabs were exposed for one month to fluorescent polystyrene beads (size: 1 μm) at nominal concentrations of 1000 or 5000 particles/mL. During the exposure, animals were maintained under controlled feeding, aeration, temperature, and photoperiod conditions. At the end of the exposure, HP and hemolymph (HL) samples were harvested for analysis of oxidative damage and total antioxidant levels. Additionally, the presence of MPs in both tissues was confirmed. Significant differences with the control groups were observed in lipid peroxidation levels in HP in animals exposed to the lowest concentration in P. clarkii and to the highest concentration in L. pugilator. A marked increase in antioxidant levels was also observed in the HL at both concentrations in P. clarkii, and at the highest MPs concentration in L. pugilator. Moreover, several histopathological changes were detected in both gills and HP, including hypertrophied lamellae, lifting or collapse of gill epithelia, loss of normal shape of hepatopancreatic tubules, and epithelial atrophy in the HP tissue. We conclude that exposure to MP beads at selected concentrations results in oxidative damage, induces histopathological changes in gills and HP, and triggers an antioxidant response in two crustacean species.

Micro- and nanoplastics transfer from seawater to the atmosphere through aerosolization under controlled laboratory conditions

Sea spray has been suggested to enable the transfer of micro- and nanoplastics (MNPs) from the ocean to the atmosphere, but only a few studies support the role of sea spray aerosols (SSAs) as a source of airborne particles. We demonstrated that MNPs are aerosolized during wave action, via SSAs, under controlled laboratory conditions. We used a mini-Marine-Aerosol-Reference-Tank (miniMART), a device that mimics naturally occurring physical mechanisms producing SSAs, and assessed the aerosolization of fluorescent polystyrene beads (0.5-10 μm), in artificial seawater. The SSAs contained up to 18,809 particles/mL of aerosols for 0.5 μm beads, with an enrichment factor of 19-fold, and 1977 particles/mL of aerosols for 10 μm beads with a 2-fold enrichment factor. Our study demonstrates that the use of the miniMART is essential to assess MNPs aerosolization in a standardized way, supporting the hypothesis which states that MNPs in the surface of the ocean may be transferred to the atmosphere.

PAIM (πM): Portable AI-enhanced fluorescence microscope for real-time target detection

We proposed a portable AI fluorescence microscope (πM) based on a webcam and the NVIDIA Jetson Nano (NJN), integrating edge computing techniques for real-time target detection. πM achieves a physical magnification of ×5 and can resolve 228.1 lp/mm USAF features. Prepared microscopic samples and fluorescent polystyrene (PS) beads can be imaged clearly. πM’s body was fabricated by a 3D printer, weighing ∼ 250 g with dimensions of 145 mm × 172 mm × 144 mm (L × W × H), costing ∼$300. It has a similar brightfield imaging quality compared to benchtop microscopes (∼$13,000). The customized convolution neural network (CNN) inside the NJN can realize feature extraction, real-time PS bead counting, and red blood cell counting without data transfer and offline image processing. Compared with two model-free image processing methods (OpenCV and CLIJ2), our CNN method is robust in bead counting at different concentrations. Six aggregated beads can be correctly counted with 80 % accuracy. Regarding feature extraction and human RBC counting, our CNN also obtained closer results to the ground truth (GT) than the CLIJ2 method (GT: 201; CNN: 196; CLIJ2: 189). With a miniature size and real-time analysis, πM has potential in point-of-care testing, field microorganism detection, and clinical diagnosis in resource-limited areas.

Effect of Polystyrene Microplastics in Different Diet Combinations on Survival, Growth and Reproduction Rates of the Water Flea (Daphnia magna)

Microplastic pollution is a problem not only in the marine environment but also in freshwater ecosystems. Water flea (Daphnia magna) is one of the most common omnivorous cladocerans in freshwater ecosystems. In this study, the potential effects of microplastics (fluorescent polystyrene beads with dimensions of 6 microns) on the survival, growth and reproduction of Daphnia magna were examined during 21 days of laboratory experiments. Microplastics (MPs) were observed to be ingested alone or along with either the microalgae Chlorella vulgaris (Cv) or baker’s yeast (By). D. magna fed exclusively with microplastics showed a drastic decline in survival similar to that in the starving group. The least growth in total length or width was observed in Daphnia specimens fed only MPs and the starved groups. Daphia fed with a mixture of MPs/Cv or MPs/By produced a significantly (p &lt; 0.05) lower number of ephippia. Our results show that high concentrations of microplastics adversely affect Daphnia magna populations.

Evidence on Invasion of Blood, Adipose Tissues, Nervous System and Reproductive System of Mice After a Single Oral Exposure: Nanoplastics versus Microplastics

ObjectiveThis study was designed to provide the evidences on the toxicokinetics of microplastics (MPs) and nanoplastics (NPs) in the bodies of mammals. Methods100 nm, 3 μm, and 10 μm fluorescent polystyrene (PS) beads were administered to mice once by gavage at a dose of 200 mg/kg body weight. The levels and change of fluorescence intensity in samples of blood, subcutaneous fat, perirenal fat, peritesticular fat, cerebrum, cerebellum, testis, and epididymis were measured at 0.5, 1, 2, and 4 h after administration using an IVIS Spectrum small-animal imaging system. Histological examination, confocal laser scanning, and transmission electron microscope were performed to corroborate the findings. ResultsAfter confirming fluorescent dye leaching and impact of pH value, increased levels of fluorescence intensity in blood, all adipose tissues examined, cerebrum, cerebellum, and testis were measured in the 100 nm group, but not in the 3 and 10 μm groups except in the cerebellum and testis at 4 h for the 3 μm PS beads. The presence of PS beads was further corroborated. ConclusionAfter a single oral exposure, NPs are absorbed rapidly in the blood, accumulate in adipose tissues, and penetrate the blood-brain/testis barriers. As expected, the toxicokinetics of MPs is significantly size-dependent in mammals.

Upgraded User-Friendly Image-Activated Microfluidic Cell Sorter Using an Optimized and Fast Deep Learning Algorithm.

Image-based cell sorting is essential in biological and biomedical research. The sorted cells can be used for downstream analysis to expand our knowledge of cell-to-cell differences. We previously demonstrated a user-friendly image-activated microfluidic cell sorting technique using an optimized and fast deep learning algorithm. Real-time isolation of cells was carried out using this technique with an inverted microscope. In this study, we devised a recently upgraded sorting system. The cell sorting techniques shown on the microscope were implemented as a real system. Several new features were added to make it easier for the users to conduct the real-time sorting of cells or particles. The newly added features are as follows: (1) a high-resolution linear piezo-stage is used to obtain in-focus images of the fast-flowing cells; (2) an LED strobe light was incorporated to minimize the motion blur of fast-flowing cells; and (3) a vertical syringe pump setup was used to prevent the cell sedimentation. The sorting performance of the upgraded system was demonstrated through the real-time sorting of fluorescent polystyrene beads. The sorter achieved a 99.4% sorting purity for 15 μm and 10 μm beads with an average throughput of 22.1 events per second (eps).

Blood uptake and urine excretion of nano- and micro-plastics after a single exposure

Nano- and micro-plastic (NMP) pollution has emerged as a global issue; however, uptake in the blood is controversial. Also, there is no evidence that NMPs are excreted via urine. This study was designed to clarify the time course of NMPs absorption in blood and the excretion in urine.Male mice received a single administration of fluorescent polystyrene (PS) beads (100-nm and 3-μm) via tail vein injection, gavage, or pulmonary perfusion. Blood and urine samples were measured 0.5, 1, 2, and 4 h after exposure by confocal laser scanning microscope (CLSM). Transmission electron microscopy (TEM) was performed to corroborate the findings.Fluorescence particles were detected in both blood and urine from the 100-nm and 3-μm PS-treated groups after exposure. In the 3-μm PS treated group, particles with corresponding diameters were detected after intravenous injection and pulmonary perfusion, and particles with a diameter <3 μm were detected in blood samples after gavage. The fluorescent signal in urine was particularly weak and the size was <3 μm. Significant time course changes in fluorescence intensity were demonstrated in blood and urine (P < 0.05) after intravenous injection and pulmonary perfusion in the 100-nm PS-treated group. By contrast, significant changes were detected in the urine (P < 0.05), but not the blood, after gavage. TEM confirmed the presence of particles with corresponding diameters in blood samples; however, the excretion in urine was difficult to confirm for nano-plastics (NPs) and micro-plastics (MPs) because all particles with diameters of approximately 100 nm and 3 μm had irregular shapes and no clear boundaries.Our findings revealed that both NPs and MPs enter the blood circulation through digestive and respiratory pathways. Both 100-nm and 3-μm NMPs may be excreted through urine, but further evidence is needed. The physical and chemical properties of MPs may be impacted by digestive processes in vivo.

Characterizing Peristalsis during a High-Fat Meal compared to a Low-Fat Meal in Zebrafish larvae (Danio rerio).

Obesity is a far too common issue in the Appalachian region. Because larval zebrafish (Danio rerio) are optically transparent up to fourteen days post fertilization (dpf) and have high synteny with humans, it makes them ideal research models to study biological responses that include metabolic and physiological processes. The intestines perform a wave like, autoregulated, continuous series of muscular contractions and relaxations called peristalsis to push food through the digestive system. Diets spiked with fluorescent polystyrene beads were used to visualize consumption of the diet to determine an optimal feeding period. Once determined, seven-dpf larvae were fed two different diets, visualizing which diet causes the most peristaltic movement. One diet was prepared with high fat contents and another with low fat contents. The larvae were fed for 3 hours at 20 minutes apart to ensure all larvae fed for the same period before being imaged. Each diet was fed to 25 larvae to allow a good sample size in analysis. Using the Zeiss Discovery V8 microscope, an image sequence was captured at 1 image per second for 5 minutes. For analysis, using NIH Image J and RStudio, we are recording pixel movement through 5 different regions of interest (ROI) in the intestinal tract of the fed larvae. For the experimental data, pixel movement will represent peristaltic movement between different diets. Analysis has yet to be concluded, but we expect the results to show that the larvae being fed the high fat diet will have a higher rate of peristaltic movement.

Food availability is crucial for effects of 1-μm polystyrene beads on the nematode Caenorhabditis elegans in freshwater sediments

Freshwater sediments represent a sink for microplastic (<5 mm) through various processes. Thus, benthic organisms can be exposed to relatively high concentrations of microplastics. Surprisingly, studies on benthic organisms are still underrepresented in the field of ecotoxicological effect assessment of microplastics. Therefore, we studied the effects of 1-μm polystyrene (PS) beads on the reproduction of the nematode Caenorhabditis elegans using a standardized protocol for toxicity testing in freshwater sediments (96 h; ISO 10872:2020), combined with ingestion experiments using fluorescent PS beads. To investigate the role of sediment properties (e.g., textures, organic contents) for ingestion and effects of PS beads, five different artificial and field-collected sediments were used.Body burdens of 1-μm PS beads in the intestinal tract of the nematodes after 96 h differed between the sediments, however, differences were not significant over the whole course of the experiment. EC10 and EC50-values of 1-μm PS beads for C. elegans’ reproduction in the various sediments ranged from 0.9 to 2.0 and 4.8 to 11.3 mg PS/g dry sediment, respectively. The ECx-values showed to be considerably higher than values reported for water exposure (EC10/50: 0.2 and 0.6 mg PS/ml, respectively), which was probably due to higher food densities in sediment compared to water exposure. Based on the PS beads/bacteria ratio, ECx-values were comparable between sediment and water exposure, suggesting that also in sediments microplastic reduces the food availability for C. elegans causing lower reproduction. This indirect effect mechanism was confirmed by experiments with varying food densities. Thus, the nutritious conditions might play a crucial role for the overall ecological risk of microplastics in benthic ecosystems.

Optical probing of local membrane potential with fluorescent polystyrene beads

The study of electrical activity in single cells and local circuits of excitable cells, such as neurons, requires an easy-to-use, high-throughput methodology that allows for the measurement of membrane potential. Investigating the electrical properties in specific subcompartments of neurons, or in a specific type of neurons, introduces additional complexity. An optical voltage-imaging technique that allows high spatial and temporal resolution could be an ideal solution. However, most valid voltage-imaging techniques are nonspecific. Those that are more site-directed require a lot of preliminary work and specific adaptations, among other drawbacks. Here, we explore a new method for membrane voltage imaging, based on Förster resonance energy transfer between fluorescent polystyrene (FPS) beads and dipicrylamine. Not only has it been shown that fluorescence intensity correlates with membrane potential, but more importantly, the membrane potential from individual particles can be detected. Among other advantages, FPS beads can be synthesized with surface functional groups and can be targeted to specific proteins by conjugation of recognition molecules. Therefore, in the presence of dipicrylamine, FPS beads represent single-particle detectors of membrane potential that can be localized to specific membrane compartments. This new and easily accessible platform for targeted optical voltage imaging can further elucidate the mechanisms of neuronal electrical activity.

Incoherent Optical Tweezers on Black Titanium.

Optical tweezers enable the manipulation of micro- and nanodielectric particles through entrapment using a tightly focused laser. Generally, optical trapping of submicron size particles requires high-intensity light in the order of MW/cm2. Here, we demonstrate a technique of stable optical trapping of submicron polymeric beads on nanostructured titanium surfaces (black-Ti) without the use of lasers. Fluorescent polystyrene beads with a diameter d = 20-500 nm were successfully trapped on black-Ti by low-intensity focused illumination of incoherent light at λ = 370 m from a Hg lamp. Light intensity was 5.5 W/cm2, corresponding to a reduced light intensity of 6 orders of magnitude. Upon switching off illumination, trapped particles were released from the illuminated area, indicating that trapping was optically driven and reversible. Such trapping behavior was not observed on nonstructured Ti surfaces or on nanostructured silicon surfaces. Thus, the Ti nanostructures were demonstrated to play a key role.

Optical Probing of Local Membrane Potential with Fluorescent Polystyrene Beads

Optical Probing of Local Membrane Potential with Fluorescent Polystyrene Beads

Constructing a high throughput novel microfluidic slide for in vivo imaging of larval zebrafish (Danio rerio).

Larval zebrafish (Danio rerio) are optically transparent up to fourteen days post fertilization (dpf) and have high synteny with Homo sapiens which makes them viable research models for the study of metabolic and physiological processes at a unicellular level. Preceding methods to image zebrafish larvae have been limited to single larvae and long-term imaging has been a challenge. The aim of this study is to design a micro-fluidic slide for mounting larvae that greatly extends the duration of time for in vivo imaging, while supplying selected drugs, modified/selected diets, and fresh embryo media. Fluorescently labeled material can be custom made or are commercially available. Examples are high fat diets spiked with fluorescently conjugated lipids such as BODIPY or fluorescent polystyrene beads. To improve upon our pervious chip design, we used the Zeiss Discovery V8 microscope to re-image five seven-dpf larvae in order to adjust the measurements of the chamber to properly accommodate an average seven-dpf larvae. The newest prototype slide is currently under testing to determine effectiveness and efficiency with fluorescent polystyrene beads to visualize consumption of a diet delivered while the larvae is in the microfluidic chip. We expect to record the transit of the beads through the intestine of the treated larvae. This work was supported by NIH Grant P20GM103434 to the West Virginia IDeA Network for Biomedical Research Excellence and NIH Grant P20GM103434 awarded to Bluefield State College and the McNair Scholars Program.

Optimization of a Single-Particle Micropatterning System With Robotic nDEP-Tweezers

In this study, a system of automatic microparticle patterning that could enable the separation, trapping, and translation of single microbeads in liquid suspension using negative dielectrophoresis (DEP) tweezers was presented to form a single-bead pattern. A microchip with integrated electrodes was flipped and placed above the substrate through a micromanipulator. Microparticles laying on the substrate could be displaced to different positions relative to the electrodes on the microchip, and only the selected particles would be trapped by the electric fields generated from electrodes. Vision-based approaches were used to evaluate the necessary information, such as the gap distance and the positions of electrodes and microparticles in the image. A strategy for separating nearby particles was proposed to achieve single-bead patterning with high accuracy. A controller was used to guide the microparticles toward the position for trapping while avoiding flow disturbance. Different strategies were simulated to decrease the patterning time and find the minimum traveling distance and the best route of movement. The optimization problem is NP-hard. Hence, global optimization algorithms, such as genetic algorithm, particle swarm optimization, and ant colony optimization (ACO), were simulated, and the results were compared with those of the local optimization method. The comparison results showed that ACO obtained the best performance among the methods. The strategy for constructing high-quality microparticle patterns was also examined through experiments. Orange fluorescent polystyrene beads suspended in 6-aminohexanoic acid solution were considered and successfully patterned on a glass substrate by using the proposed system. <i>Note to Practitioners</i>&#x2014;Micropatterning is an effective tool for pharmaceutical research and drug discovery. However, the reliability of results depends on the quality of patterns. Existing approaches, such as microfluidic devices, are limited to create a pattern from one chip for single use, and the entire process is sealed and isolated from the environment. In this study, a multielectrode microchip combined with a vision-based micromanipulator is introduced to create a novel noncontact approach for microparticle patterning, which offers high flexibility and guarantees the quality of the constructed patterns. The electrodes on the chip can be selectively energized to determine the shape of the final pattern. A real-time screening is performed so that the micromanipulator will only guide the particles in good condition for selection. An optimization algorithm is implemented to aid the particle selection with the electrodes, allowing high-quality microparticle patterns to be constructed in a short time for various applications.