In this paper, a series of B2O3-Al2O3-ZnO-BaO-Tm2O3-Tb2O3 glasses were synthesized by high temperature melting method. Under appropriate ultraviolet excitation, Tm3+ ions produce 1D2→3F4 energy level transition and emit blue light. The concentration quenching point is 0.75% mol, and the color saturation is high. Tb3+ ions produce 5D4→7F5 energy level transition and emit green light, and the concentration quenching point of Tb3+ ions is 0.75% mol. By characterizing the fluorescence lifetime and luminescence intensity of B2O3-Al2O3-ZnO-BaO-Tm2O3-Tb2O3 glass, the existence of Tm3+→Tb3+ energy transfer in the glass was verified, and the energy transfer efficiency and energy transfer mechanism were studied in detail. It is found that B2O3-Al2O3-ZnO-BaO-Tm2O3-Tb2O3 glass is an ideal material for light-emitting diodes, light-emitting materials and fluorescent display devices.