Fluidic System Research Articles

High Efficiency Shear-Driven Nanofluidic System for Energy Conversion/Harvesting.

In this work, we propose a shear-driven nanofluidic system for energy harvesting/conversion. The system consists of a nanochannel formed by two parallel walls, where the lower wall is negatively charged, while the upper wall is neutral. The motion of the upper wall caused by a shear force drives the solution in the fluidic system to move, which generates an ionic current due to the migration of excess cations in the system. Molecular dynamics simulations demonstrate that the efficiency of the system is affected by the wall charge density, shearing stress, channel height, and binding energy of the walls. The effects of these factors on the efficiency are studied. In particular, it is shown that a high binding energy for the upper wall (e.g., hydrophilic wall) can reduce the flow slip at the upper wall and effectively transfer energy from the wall to the fluid. For the lower wall, a low binding energy, which corresponds to a hydrophobic wall, can reduce the friction at the wall, enhance the flow velocity, and improve the energy conversion efficiency. By varying these parameters, it is found that the maximum energy conversion efficiency of the system reaches 65.8%, which is the highest compared with previous systems. The underlying mechanisms are explained using the slip length at the walls, wall velocity, and charge density profiles. The system proposed in this work provides insights into the design of nanofluidic systems for energy harvesting/conversion.

Cetuximab scFv-Modified 5-FU Loaded Chitosan Nanoparticles: "A Novel Therapeutic Platform."

Colorectal cancer [CRC] is among the most fatal types of cancer. An active targeting delivery system that specifically interacts with CRC cells could improve the therapy's outcomes. Herein, Cetuximab single-chain fragment variable antibody [scFv] fragments were conjugated to the surface of 5-FU encapsulated chitosan nanoparticles [CS NPs] to develop an effective therapeutic platform [scFv-CS/5-FU NPs]. CS/5-FU NPs were synthesized using a special fluidic system. Encapsulation efficiency [EE], loading capacity [LC], and the drug release profile of the particles were determined. scFv fragments were produced recombinantly and tailored on the surface of CS/5-FU NPs. The physicochemical features of scFv-CS/5-FU NPs were also characterized. MTT and flow cytometry assay investigated the toxicity effect of scFv-CS/5-FU NPs on the HCT116 cell line. CS/5-FU NPs had a homogenous spherical shape. They possessed sustainable drugrelease behavior. The produced scFv-CS/5-FU NPs were also spherical. scFv-CS/5-FU NPs significantly decreased the viability of cancerous cells in a dose-dependent manner and induced apoptosis in 97.97% of targeted cells. scFv-CS/5-FU NPs showed remarkable anti-CRC activity. This novel targeting delivery system reduced the effective dose of 5-FU which is of vital importance to decrease the devastating side effects of chemotherapy.

Framework for Microdosing Odors in Virtual Reality for Psychophysiological Stress Training.

To better cope with stress in emergencies, emergency personnel undergo virtual reality (VR) stress training. Such training typically includes visual, auditory and sometimes tactile impressions, whereas olfactory stimuli are mostly neglected. This concept paper therefore examines whether odors might be beneficial for further enhancing the experience of presence and immersion into a simulated environment. The aim is to demonstrate the benefits of VR civilian stress training for emergency personnel and to investigate the role of odors as stressors by manipulating the degree of perceived psychophysiological stress via olfactory impressions. Moreover, the current paper presents the development and validation of a convenient and portable fragrance dosing system that allows personalized odor presentation in VR. The presented system can transport reproducible small quantities of an air-fragrance mixture close to the human nose using piezoelectric stainless steel micropumps. The results of the fluidic system validation indicate that the micropump is suitable for releasing odors close to the nose with constant amounts of odor presentation. Furthermore, the theoretical background and the planned experimental design of VR stress training, including odor presentation via olfactory VR technology, are elucidated.

Temperature-triggered liquid metal actuators for fluid manipulation by leveraging phase transition control

ABSTRACT Small-scale pumps for controlling microfluidics have promising applications in drug delivery and chemical assays. Liquid metal (LM) demonstrates excellent flow pumping performance due to its simple structure and the electrocapillary effect under an electric field. However, LM droplets risk escaping from constrained structures, which can lead to pump failure. Temperature regulation is also a critical parameter in optimizing chemical reactions in fluidic systems, however, integrating it into a compact system remains challenging. Here, we develop a temperature-triggered gallium-based actuator (TTGA) by introducing a gallium (Ga) droplet wetted on a copper (Cu) plate as the core element for flow actuation. The Cu plate prevents the Ga droplet from escaping the chamber and significantly increases the flow rate. By leveraging the electrochemical method to inhibit the supercooling effect of Ga, the TTGA enables activation/deactivation for flow actuation at different temperatures. We investigate the impact of electrode position, solution concentration, and applied voltage on TTGA’s pumping efficiency. By dynamically tuning the Ga droplet’s temperature to control phase transition, TTGA allows for accurate flow actuation control. Furthermore, placing Ga and eutectic Ga-indium (EGaIn) droplets in different channels enables the expected flow divergence for fluids with different temperatures. The development of TTGA presents new opportunities in microfluidics and biomedical treatment.

On the design and fabrication of nanoliter-volume hanging drop networks

Hanging drop cultures provide a favorable environment for the gentle, gel-free formation of highly uniform three-dimensional cell cultures often used in drug screening applications. Initial cell numbers can be limited, as with primary cells provided by minimally invasive biopsies. Therefore, it can be beneficial to divide cells into miniaturized arrays of hanging drops to supply a larger number of samples. Here, we present a framework for the miniaturization of hanging drop networks to nanoliter volumes. The principles of a single hanging drop are described and used to construct the fundamental equations for a microfluidic system composed of multiple connected drops. Constitutive equations for the hanging drop as a nonlinear capacitive element are derived for application in the electronic-hydraulic analogy, forming the basis for more complex, time-dependent numerical modeling of hanging drop networks. This is supplemented by traditional computational fluid dynamics simulation to provide further information about flow conditions within the wells. A fabrication protocol is presented and demonstrated for creating transparent, microscale arrays of pinned hanging drops. A custom interface, pressure-based fluidic system, and environmental chamber have been developed to support the device. Finally, fluid flow on the chip is demonstrated to align with expected behavior based on the principles derived for hanging drop networks. Challenges with the system and potential areas for improvement are discussed. This paper expands on the limited body of hanging drop network literature and provides a framework for designing, fabricating, and operating these systems at the microscale.

Essential Fluidics for a Flow Cytometer.

Flow cytometry is an inherently fluidic process that flows particles on a one-by-one basis through a sensing region to discretely measure their optical and physical properties. It can be used to analyze particles ranging in size from nanoparticles to whole organisms (e.g., zebrafish). It has particular value for blood analysis, and thus most instruments are fluidically optimized for particles that are comparable in size to a typical blood cell. The principles of fluid dynamics allow for particles of such size to be precisely positioned in flow as they pass through sensing regions that are tens of microns in length at linear velocities of meters per second. Such fluidic systems enable discrete analysis of cell-sized particles at rates approaching 100 kHz. For larger particles, the principles of fluidics greatly reduce the achievable rates, but such high rates of data acquisition for cell-sized particles allow rapid collection of information on many thousands to millions of cells and provides for research and clinical measurements of both rare and common cell populations with a high degree of statistical confidence. Additionally, flow cytometers can accurately count particles via the use of volumetric sample delivery and can be coupled with high-throughput sampling technologies to greatly increase the rate at which independent samples can be delivered to the system. Due to the combination of high analysis rates, sensitive multiparameter measurements, high-throughput sampling, and accurate counting, flow cytometry analysis is the gold standard for many critical applications in clinical, research, pharmaceutical, and environmental areas. Beyond the power of flow cytometry as an analytical technique, the fluidic pathway can be coupled with a sorting mechanism to collect particles based on desired properties. We present an overview of fluidic systems that enable flow cytometry-based analysis and sorting. We introduce historical approaches, explanations of commonly implemented fluidics, and brief discussions of potential future fluidics where appropriate. © 2024 Wiley Periodicals LLC.

Additive manufacturing of personalized scaffolds for vascular cell studies in large arteries: A case study on carotid arteries in sickle cell disease patients

Patient-specific models have increasingly gained significance in medical and research domains. In the context of hemodynamic studies, computational fluid dynamics emerges as a highly innovative and promising approach. We propose to augment these computational studies with cell-based experiments in individualized artery geometries using personalized scaffolds and vascular cell experiments. Previous research has demonstrated that the development of Sickle Cell Disease (SCD)-Related Vasculopathy is dependent on personal geometries and flow characteristics of the carotid artery. This fact leaves conventional animal experiments unsuitable for gaining patient-specific insights into cellular signaling, as they cannot replicate the personalized geometry. These personalized dynamics of cellular signaling may further impact disease progression, yet remains unclear. This paper presents a six-step methodology for creating personalized large artery scaffolds, focusing on high-precision models that yield biologically interpretable patient-specific results. The methodology outlines the creation of personalized large artery models via Additive Manufacturing suitably for supporting cell culture and other cellular experiments. Additionally, it discusses how different Computer-Aided-Design (CAD) construction modes can be used to obtain high-precision personalized models, while simplifying model reconfigurations and facilitating adjustments to general designs such as system connections to bioreactors, fluidic systems and visualization tools. A proposal for quality control measures to ensure geometric congruence for biological relevance of the results is added. This innovative, interdisciplinary approach appears promising for gaining patient-specific insights into pathophysiology, highlighting the importance of personalized medicine for understanding complex diseases.

Design, modeling, and characteristics of ring-shaped robot actuated by functional fluid

The controlled actuation of hydraulic and pneumatic actuators has unveiled fresh and thrilling opportunities for designing mobile robots with adaptable structures. Previously reported rolling robots, which were powered by fluidic systems, often relied on complex principles, cumbersome pump and valve systems, and intricate control strategies, limiting their applicability in other fields. In this investigation, we employed a distinct category of functional fluid identified as Electrohydrodynamic (EHD) fluid, serving as the pivotal element within the ring-shaped actuator. A short stream of functional fluid is placed within a fluidic channel and is then actuated by applying a direct current voltage aiming at shifting the center of mass of the robot and finally pushed the actuator to roll. We designed a ring-shaped fluidic robot, manufactured it using digital machining methods, and evaluated the robot’s characteristics. Furthermore, we developed static and dynamic models to analyze the oscillation and rolling motion of the ring-shaped robots using the Lagrange method. This study is anticipated to contribute to the expansion of current research on EHD flexible actuators, enabling the realization of complex robotic systems.

Improved platelet separation performance from whole blood using an acoustic fluidics system.

This study investigated the effectiveness of acoustic separation for platelet analysis in patients with non-small-cell lung cancer (NSCLC), comparing it with traditional centrifugation methods. In total, 10 patients with NSCLC and 10 healthy volunteers provided peripheral blood samples, which were processed using either acoustic separation or centrifugation to isolate platelets. The study included whole transcriptome analysis of platelets, peripheral blood mononuclear cells, and tumor tissue samples, employing hierarchical clustering and Gene Ontology analysis to explore gene expression differences. Acoustic separation proved more efficient than centrifugation in terms of platelet yield, recovery rate, and RNA yield. Gene expression profiles of platelets from patients with NSCLC showed distinct patterns compared with healthy volunteers, indicating tumor-influenced alterations. Gene Ontology analysis revealed enrichment in pathways associated with platelet activation and the tumor microenvironment. This finding indicates the potential of acoustic isolation in platelet separation and its relevance in understanding the unique gene expression profile of platelets in patients with NSCLC. The findings of this study suggested that platelets from cancer patients separated by acoustic techniques exhibited tumor-specific alterations and provided new insights into the diagnosis of cancer in platelet analysis systems in clinical practice.

Electrobiofabrication of antibody sensor interfaces within a 3D printed device yield rapid and robust electrochemical measurements of titer and glycan structure.

We report the integration of 3D printing, electrobiofabrication, and protein engineering to create a device that enables near real-time analysis of monoclonal antibody (mAb) titer and quality. 3D printing was used to create the macroscale architecture that can control fluidic contact of a sample with multiple electrodes for replicate measurements. An analysis "chip" was configured as a "snap-in" module for connecting to a 3D printed housing containing fluidic and electronic communication systems. Electrobiofabrication was used to functionalize each electrode by the assembly of a hydrogel interface containing biomolecular recognition and capture proteins. Specifically, an electrochemical thiol oxidation is used to assemble a thiolated polyethylene glycol hydrogel, that in turn is covalently coupled to either a cysteine-tagged protein G that binds the antibody's Fc region or a lectin that binds the glycans of target mAb analytes. We first show the design, assembly, and testing of the hardware device. Then, we show the transition of a step-by-step sensing methodology (e.g., mix, incubate, wash, mix, incubate, wash, measure) into the current method where functionalization, antibody capture, and assessment are performed in situ and in parallel channels. Both titer and glycan analyses were found to be linear with antibody concentration (to 0.2 mg/L). We further found the interfaces could be reused with remarkably similar results. Because the interface assembly and use are simple, rapid, and robust, we suggest this assessment methodology will be widely applicable, including for other biomolecular process development and manufacturing environments.

Role of the constriction angle on the clogging by bridging of suspensions of particles

Confined flows of particles can lead to clogging, and therefore failure, of various fluidic systems across many applications. As a result, design guidelines need to be developed to ensure that clogging is prevented or at least delayed. In this Letter, we investigate the influence of the angle of reduction in the cross section of the channel on the bridging of semidilute and dense non-Brownian suspensions of spherical particles. We observe a decrease of the clogging probability with the reduction of the constriction angle. This effect is more pronounced for dense suspensions close to the maximum packing fraction where particles are in contact in contrast to semidilute suspensions. We rationalize this difference in terms of arch selection. We describe the role of the constriction angle and the flow profile, providing insights into the distinct behavior of semidilute and dense suspensions. Published by the American Physical Society 2024

Untethered soft magnetic pump for microfluidics-based Marangoni surfer

Microfluidics has enabled the miniaturization of fluidic systems for various biomedical and industrial applications, including small-scale robotic propulsion. One mechanism for generating propulsive force through microfluidics is by exploiting the solutal Marangoni effect via releasing surfactant on the air-water interface. Surfactants locally reduce the surface tension, which leads to a surface stress that can propel the floating robot, called Marangoni surfer. However, so far the release of the surfactant is not controllable. In this study, we combine microfluidics-based Marangoni propulsion with a novel untethered magnetic pumping mechanism to enhance its controllability. The proposed magnetic micropump capitalizes on the interaction force between two soft magnets, which can generate a pumping force of 4.64 mN to actuate a membrane, and achieve a deformation of 450 μm. Net flow is achieved using a nozzle/diffuser flow rectifier whose efficacy as a function of the channel geometry is numerically studied. We investigate the flow rate of the pump with regard to the actuation frequency. Finally, we demonstrate its ability to control the motion of the Marangoni surfer.

Safety and prognosis of phacoemulsification using active sentry and active fluidics with different IOP settings - a randomized, controlled study

PurposeTo explore the impact of different intraoperative intraocular pressure (IOP) settings on the safety and prognosis in phacoemulsification.MethodsAge related cataract patients who met the inclusion criteria and underwent phacoemulsification by using active sentry handpiece and active fluidics system. According to different intraoperative IOP settings during surgery, they were randomly divided into two groups: the 20mmHg group and the 60mmHg group. The best corrected visual acuity (BCVA), cumulative dissipated energy (CDE), total U/S time, active surge mitigation (ASM), estimated fluid usage (EFU) as well as the changes in corneal thickness (CT), corneal epithelial layer thickness (CELT) and endothelial cell density (ECD) were collected. The post-operative follow-up was only 1 day.ResultsA total of 110 cases (110 eyes) were included in the study. There were 55 eyes in each group. There was no statistically significant difference in postoperative BCVA (p = 0.839). The CDE, total U/S time and EFU during surgery were (5.22 ± 3.31), (30.60 ± 15.06), (45.07 ± 12.68) and (4.70 ± 2.83), (27.39 ± 13.75), (42.38 ± 11.93) in the 20mmHg group and 60mmHg group (p = 0.381, 0.246, 0.254). The ASM during surgery in the 20mmHg group and 60mmHg group were (0.95 ± 2.77) and (7.24 ± 6.34), respectively. The 20mmHg group showed a significant decrease in ASM (p < 0.001). There was no statistically significant difference in the changes in CT, CELT and ECD before and after surgery between the two groups (p = 0.913, 0.825, 0.624). Both groups did not experience any intraoperative complications, such as posterior capsule rupture.ConclusionA lower IOP setting of 20 mmHg can significantly reduce the occurrence of intraoperative surges during phacoemulsification. And there was no increase in rate of complications.Trial RegistrationThe trial registration number is ChiCTR2100050240. The registered date is August 24th, 2021.

3D-Printed Hierarchical Electrodes for Ampere-Level Alkaline Water Electrolysis

In the realm of water electrolysis technology, Alkaline Electrolysis Cell (ALK) hydrogen production stands as a pivotal technology, playing a critical role in the pursuit of large-scale green hydrogen production and the achievement of the "dual carbon" objective. Despite its importance, the mass transfer efficiency of commercial porous nickel electrodes currently used in ALK systems is suboptimal. These electrodes, plagued by unstable catalyst loading, typically operate at current densities between 300-500 mA/cm², seldom reaching the desired threshold of 1 A/cm². This limitation hampers the potential of ALK hydrogen production to satisfy industrial-scale hydrogen production demands.In the quest to overcome these limitations, the concept of the Triple Periodic Minimum Surface (TPMS) structure, a cellular architecture mathematically defined and prevalent in natural biological forms such as butterfly wings and beetle shells, is introduced. The TPMS's unique geometry is adept at adapting to mechanical requirements for various applications, including structural and fluidic systems. It allows for versatile manipulation of geometric parameters like aperture size, porosity, and tortuosity, thereby substantially broadening the design possibilities for electrode structures and enhancing the efficiency of gas-liquid and electron transfer.To harness these benefits, we propose the creation of nickel/nickel-iron electrodes using 3D printing technology. These electrodes are characterized by their cross-scale porosity, ordered structure, and adjustable TPMS design. This innovative electrode design significantly augments bubble transport efficiency and mass transfer performance, while also providing additional anchor points for catalysts, thereby enhancing the electrochemical active surface area, intrinsic activity, and stability of the composite catalyst. As a result, these electrodes achieve ampere-level current densities in ALK electrolysis cells, enhancing electrolysis efficiency and reducing energy consumption.Our methodology involves the use of selective laser melting printing technology to fabricate multi-tier nickel/nickel-iron electrodes of varying specifications. These electrodes are then coated with MOOH catalysts using an electrochemical deposition process. Through rigorous testing and comparison of electrochemical performance parameters, we identified the optimal multi-stage nickel/nickel-iron electrode and its composite catalyst.Electrochemical performance tests reveal that, compared to traditional commercial porous nickel electrodes, our 3D printed electrodes decrease overpotential by 14% and improve electrolytic cell efficiency by 10% at a current density of 1 A/cm². Furthermore, these electrodes exhibit minimal degradation after a 500-hour durability test. The demonstrated efficiency and durability of these 3D-printed hierarchical electrodes indicate significant potential for their application in ampere-level alkaline water electrolysis. Figure 1

A Stretchable Soft Pump Driven by a Heterogeneous Dielectric Elastomer Actuator

AbstractElectrically driven soft pumps act as ideal “hearts” for flexible fluidic systems in soft robots and wearable devices. Dielectric elastomers (DEs) are promising for soft pump fabrication owing to their features of fast response, large strain, high energy density, and low power consumption. However, conventional dielectric elastomer actuators (DEAs) using single DE material usually exhibit poor pumping performance due to electromechanical instability or insulating problems. Herein, a multilayer structured heterogeneous dielectric elastomer actuator (H‐DEA) is fabricated based on thin films of processable, high‐performance dielectric elastomer (PHDE) and silicones, and is integrated onto silicone pump body to build a fully soft pump. The pump achieves a flow rate of 3.25 mL min−1 and a blocked pressure of 2.75 kPa with a mass of less than 1 g and a power consumption of 0.21 W. It maintains pumping functions when being bent or stretched and after twisted. It works for viscous liquids and is demonstrated to drive a soft robotic fluidic circuit.

Machine Learning Investigation For Tri-Magnetized Sutterby Nanofluidic Model with Joule Heating In Agrivoltaics Technology

This paper aims to establish a panoramic foundation for investigating the impact of sunlight on a recently formulated water-based tri-nano hybrid Sutterby liquid (TNHF) under provided magnetic field, directed through photovoltaic solar panels by exploiting the knacks of machine intelligent computing paradigm. The comparative performance of hybrid and tri-nano fluidic system with water as a base fluid is exhaustively analyzed and discussed. This research is unique, as it has a distinguished figurative comparison analysis between two different types of nanofluidic materials, which helps to choose the best one for use in agrivoltaics systems, and replace the materials already in use to enhance the efficiency and performance coefficients. Furthermore, the description of the composition scheme makes this research more feasible and applicable. A numerical dataset of nonlinear mathematical model is generated by employing finite difference scheme in the recently introduced Python bvp-solver algorithm, then it is embedded into artificial intelligence (AI)-based Levenberg Marquardt neural network algorithm (LMNNA). A significant outcome of the research indicates that the integration TNHF results in a notably faster enhancement of heat transfer rate and temperature framework as compared to traditional hybrid fluid. It is observed that introducing three distinct nanomaterials of specific thermophysical characteristics enhances the thermal exchanging profile and faces an obvious flow rate dissipation in solar plate channels. The standard numerical and AI-generated results are documented to portray the stability, accuracy and efficacy of scheme in terms of iterative learning curves on MSE, error analysis, histograms and regression statistics. Additional perquisites of the methodology include cost effectiveness, time-saving ability, robustness, stability and its extendibility.

Nanofluidic Ionic Memristors.

Living organisms use ions and small molecules as information carriers to communicate with the external environment at ultralow power consumption. Inspired by biological systems, artificial ion-based devices have emerged in recent years to try to realize efficient information-processing paradigms. Nanofluidic ionic memristors, memory resistors based on confined fluidic systems whose internal ionic conductance states depend on the historical voltage, have attracted broad attention and are used as neuromorphic devices for computing. Despite their high exposure, nanofluidic ionic memristors are still in the initial stage. Therefore, systematic guidance for developing and reasonably designing ionic memristors is necessary. This review systematically summarizes the history, mechanisms, and potential applications of nanofluidic ionic memristors. The essential challenges in the field and the outlook for the future potential applications of nanofluidic ionic memristors are also discussed.

Experimental study on the periodic pulsating ventilation by fluidic oscillator on pollutant dispersion and ventilation performance in enclosed environment

Ventilation plays an essential role in creating healthy and comfortable indoor environment, while adopting unsteady airflow field has the potential to improve the indoor air mixing effect, which is conducive to the dispersion and eventually the discharge of indoor pollutants. A fluidic oscillator capable of producing periodic pulsating airflow is employed to conduct a ventilation experiment to test the performance of such unsteady flow regime. The distribution of indoor pollutants at different source locations is compared under both steady and unsteady ventilation mode. The assessment of the fluidic oscillator ventilation system performance in eliminating pollutants involves the concentration exceedance rate, the decay of indoor pollutant concentration, and the resultant ventilation efficiency. At a relatively lower air supply rate, the fluidic oscillator device can enhance indoor air mixing, leading to a reduction of the peak indoor pollutant concentration. Additionally, the fluidic oscillator air terminal significantly prolongs the time needed to reach the pollutant concentration threshold, increases ventilation efficiency, and improves indoor air quality.

Optimum Design of Coaxial Hydraulic Sealing Systems Made from Polytetrafluoroethylene and Its Compounds

Fluidic actuation systems are optimizable as to energy consumption by reducing the friction in the hydraulic cylinders. Polymeric materials with special antifriction properties and good resistance to hydraulic fluids can be deployed to enhance the performance of hydraulic cylinders. Small friction forces can also be ensured by facilitating the hydrodynamic separation of the elements of the friction tribosystem, namely the seal and sealed-off surface, respectively. The study presented in this paper analyzed the hydrodynamic separation phenomenon in hydraulic cylinders with coaxial sealing systems of the pistons. The process underlying the forming of the fluid film between the seal and its contact surface was considered and the formula for calculating film thickness was deduced. This paper presents graphs that describe the variation of the fluid film thickness versus the dimensional and material characteristics of the sealing systems. The study yielded recommendations as to the most adequate polymeric material to be used and the optimum dimensional characteristics of the seal.

Parallel detection of multiple biomarkers in a point-of-care-competent device for the prediction of exacerbations in chronic inflammatory lung disease

Sudden aggravations of chronic inflammatory airway diseases are difficult-to-foresee life-threatening episodes for which advanced prognosis-systems are highly desirable. Here we present an experimental chip-based fluidic system designed for the rapid and sensitive measurement of biomarkers prognostic for potentially imminent asthma or COPD exacerbations. As model biomarkers we chose three cytokines (interleukin-6, interleukin-8, tumor necrosis factor alpha), the bacterial infection marker C-reactive protein and the bacterial pathogen Streptococcus pneumoniae—all relevant factors in exacerbation episodes. Assay protocols established in laboratory environments were adapted to 3D-printed fluidic devices with emphasis on short processing times, low reagent consumption and a low limit of detection in order to enable the fluidic system to be used in point-of-care settings. The final device demonstrator was validated with patient sample material for its capability to detect endogenous as well as exogenous biomarkers in parallel.