Finding an effective way to link the fault movement to petroleum escape is key to understand the potential of petroleum exploration in the basin. The Ordos Basin is an important petroliferous basin in China, which encompasses a world-class uncompartmentalized Jingbian natural gas field in the Ordovician marine carbonate reservoir. However, it is not clear whether the unique exhumation history and the Cenozoic faulting are responsible for the formation of the large-scale oil-gas fields within Ordovician carbonate strata in the SW basin. The combination of fault-related calcite U-Pb dating and fluid inclusions analysis offers a unique opportunity for tracking petroleum leakage along the brittle fault zone within the carbonate strata. The calcite samples were collected in the SW Ordos Basin from the carbonate fault zone and cross the Ordovician carbonate, which show clear positive Eu, slight positive Y anomalies, non-positive La and Gd anomalies, lack of negative Ce. The ratios of Y/Ho range from 29 to 36 and the average value is 32. These calcites originated in a hydrothermal environment during fault activation and yielded U-Pb ages of 32.8±3.3 Ma and 23.7±7.4 Ma. The Raman spectroscopy reveals existence of CH4 gas within the fluid inclusions coeval with the fault-related calcite. The homogenization temperatures (Th) of aqueous inclusions coeval with CH4 inclusion from two calcite veins range from 140.0-172.4°C (average Th= 157.8°C, n=16) and 127.7-154.3°C (average Th= 142.2°C, n=18). Low temperature thermochronology suggest for a rapid uplift since 40 Ma that could be associated with the activity along these faults. The Cenozoic erosion rate was 0.06 mm/y during the ∼9 Myr period from 32.8 to 23.7 Ma in the SW Ordos Basin. These Cenozoic faulting might provide a permeable leakage pathway for petroleum release into the atmosphere. This work provided an explanation for the temporal association between Cenozoic uplift and faulting, potentially leading to petroleum losses in SW Ordos Basin.