The constant temperature in laboratories is crucial for precise low-temperature measurements. Currently, there are no reports on the specific temperature needs for such experiments or quantitative analyses of how room temperature fluctuations affect cryostat control. This paper proposes a method to quantitatively analyze these fluctuations and their impact on cryostat temperature control. Taking the cryostat using for 2 K-5 K thermodynamic temperature measurement as the research object, and conducts model simulation and experimental validation. The research results indicate the primary influence on the sphere’s temperature is the time-dependent fluctuation of the room temperature. As temperature fluctuations propagate from the cryostat’s outer casing to the sphere, heat conduction is the dominant factor. The temperature fluctuations inside the system caused by environmental temperature fluctuations can be quantitatively expressed by a simple linear formula, with the coefficient being the fluctuation attenuation rate, which is 6.590 × 10-4 in this system. The deviation between experimental results and simulation results is within 5 %. To keep the sphere’s ambient temperature influence below 0.077mK, or 20 % of cryocooler-induced fluctuations, the room temperature must be controlled within 0.12 °C. In addition, the fluctuation period of room temperature is controlled below 1 h, the 0th flange is thickened, the rod is lengthened, and the material of the rod is changed to G10 above 4.2 K and stainless steel below 4.2 K can also effectively attenuate the influence on system temperature control.
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