Monitoring seepage by temperature tracer method has been widely used, where the vertical flow has been proven to be an indispensable factor. However, current research still lacks adequate consideration of it, especially for the natural convection caused by active heating. In this paper, theoretical derivation and experiments are both conducted, a two-dimensional dimensionless temperature rise–seepage velocity formula considering the natural convection is derived. Meanwhile, through the experiment system and a multi-temperature measuring sheets device, heating and temperature measurement experiments are carried out under calm water and various flow velocities ranging from 10−6 cm s−1 to 10−3 cm s−1. By comparing the temperature–time curves of different positions at different heating times in calm water, the mechanism of natural convection caused by active heating is researched. Based on this, several temperature measuring sheets are chosen, the formulas of different positions are obtained by fitting, relative influence to temperature rise–flow velocity patterns of different positions are studied. This study is of great significance for further understanding the temperature-velocity relationship, and for the design of heating-temperature measuring device used in seepage velocity monitoring with active heating.
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