On 22 May 2021, an MW 7.4 earthquake occurred in Maduo County, Qinghai Province, China, which is located on the Kunlun Mountain Pass-Jiangcuo fault inside the Bayan Har block, providing a good opportunity to investigate seismogenesis of large intraplate earthquakes. We analyze two years of continuous seismic data from June 2021 to June 2023, which were recorded at 34 portable seismic stations of the MaduoArray deployed in the source zone by our group. The LOC-FLOW workflow of automatic detection and location is applied to construct a complete and high-precision seismic catalog for the region, which includes machine-learning phase picking (PhaseNet), earthquake phase association (REAL), velocity model updating and station correction (VELEST), absolute earthquake location (HypoInverse), and relative location (HypoDD). As a result, 78,832 Maduo aftershocks and other local earthquakes are detected and relocated precisely. Our results show that the length of the Maduo aftershock zone is ∼170 km, which is mainly distributed along the NWW-SEE oriented Kunlun Mountain Pass-Jiangcuo fault, and there is a horsetail bifurcation feature at the eastern end of the aftershock sequence. The seismogenic fault is nearly vertical, and local seismicity occurs on both sides of the fault. Our results also show that there is no seismic gap or aftershock sparse area in the region. Previous studies have revealed a low-velocity and high-conductivity anomaly below the source zone, reflecting fluids ascending from the lower crustal flow. These results provide new insights into the cause of the 2021 Maduo earthquake.