This study is an elaboration on flow instabilities observed during flow boiling experiments conducted onboard the International Space Station (ISS) as part of the Flow Boiling and Condensation Experiment (FBCE). During highly subcooled flow boiling, liquid backflow into the channel that rapidly condenses vapor within the channel was observed. Experiments were conducted with an enhanced sampling frequency of 30 Hz and an extended image sequence recording duration of at least 4 seconds at 500 frames per second to further investigate the instability. Identical experiments were performed both in microgravity onboard the International Space Station (ISS) and in Earth gravity during vertical upflow. Instabilities in microgravity are more severe than those in Earth gravity and, at their most severe, propagate to the channel's upstream region. Instabilities are observed within the channel when the intensity, I, which is dependent on inlet pressure fluctuations and mass velocity, exceeds 1.8 × 105 W/m2. Parametric trends of the frequency and amplitude of inlet pressure fluctuations during instability are examined, which reveal instabilities are most severe at low flow rates, high inlet subcoolings, and high heat fluxes. Various stability maps proposed in the literature are evaluated against the present database, and instabilities only manifest for subcooling numbers greater than 14. A subset of the database containing the Onset of Flow Instability (OFI) point is extracted to first evaluate correlations available in the literature and then develop a new correlation. The new correlation is applicable in both microgravity and Earth gravity conditions and predicts the database with a Mean Absolute Error (MAE) of 1.3%.