Microplastics have been identified as a potentially emerging threat to water environment and human health. Therefore, there is a pressing demand for effective strategies to remove microplastics from water. Hydrocyclone offers a rapid separation and low energy consumption alternative but require reduction of microparticle entrainment by short flow, which limits the effectiveness for small density differentials and ultralow concentrations separation. We proposed an enhanced mini-hydrocyclone with overflow microchannels (0.72 mm width) based on the active control of short flow in hydrocyclone for microplastic removal from water. The overflow microchannels effectively redirect the particles that would typically be entrained by the short flow, leading to higher separation efficiency. Simulation results show overflow microchannels effectively reduced short flow to 0.7 %, a reduction of up to 94 % compared to conventional hydrocyclones. The hydrocyclone with overflow microchannel demonstrated a removal efficiency exceeding 98 % for 8 μm plastic microbeads at ultralow concentrations (10 ppm), which is a 33.7 % improvement over conventional hydrocyclone. Compared with other methods (e.g., filtration, adsorption, coagulation) for microplastic removal, this work achieves rapid separation capability and long period operation, highlighting hydrocyclone as a promising approach for microplastic removal in industry-scale water treatment.