The assessment of physiological quality and health in maize seeds is crucial for selecting high-quality genotypes suitable for planting. However, this quality can vary due to different variables, including genotype or endosperm composition. Therefore, the present study aimed to establish a relationship between the physiological quality and health of the nine evaluated maize genotypes. Special attention was given to the dimensions of flint and floury endosperms, following protocols described by ISTA. Additionally, the study aimed to understand the interaction between each assessed variable and its influence on the performance of crops adapted to the Central High Valleys region of Mexico. The results of this study demonstrated that seeds with flint endosperms exhibited superiority in their physiological quality and health compared to those with floury endosperms.