Floristic Survey Research Articles

Biodiversity Assessment of Trees and Shrubs at Tirunelveli’s Science Centre in Southern India: A Conservation Perspective

A comprehensive taxonomical floristic survey conducted at the District Science Centre in Tirunelveli identified 84 species of trees and shrubs, documenting each plant species with detailed information on medicinal uses, family classification, commercial importance, and population count of individual plants. The survey identified 33 families and 68 genera, with Fabaceae being the most dominant family, followed by Rubiaceae, Arecaceae, and Malvaceae. Additionally, the survey highlighted a list of vulnerable, threatened, and endangered tree species such as Cycas beddomei Dyer., Santalum album L., emphasizing the need for conservation efforts based on their spatial reach and anthropogenic pressures. These species face various threats, including loss of habitat, agriculture, pollution load, and recreational disturbances, and were assessed according to the IUCN Red List criteria. This study provides valuable insights for botanists, conservationists, and policymakers, promoting sustainable management and protection of plant biodiversity in the region.

Benefit of weeds for crop-plant mycobiota in agroecosystems: Integrating ecological demonstration and management applicability

Agricultural intensification reduces not only biodiversity in agroecosystems but also key ecosystem functions such as soil fertility. By reintroducing biological diversity in fields, weeds may enhance soil biological fertility through their influence on crop microbiota. However, letting weeds grow in crop fields will depend on weed competitiveness, farmers’ perception and acceptance, and on crop management, which influences the occurrence and the abundance of weeds in the field. This study assessed the use of eight weed species to modify wheat plant root endospheric mycobiota, and the applicability of using these plants for mycobiota enrichment in the field. By combining controlled lab experiments and field studies, we demonstrated that weeds act either as a refuge for a high diversity of fungi or as a vector for transferring fungi to the crop, particularly symbionts. Weeds differed in their competitive effect on wheat growth and only three species significantly reduced wheat growth. Interviews with farmers’ revealed that weed species were better known and more appreciated in crop fields by organic farmers than by conventional farmers. Floristic surveys confirmed that both weed occurrence and cover were higher in organic fields than in conventional fields. A multicriteria analysis showed that Trifolium repens and V. persica had the highest potential for promoting wheat plant mycobiota. Among the weed species tested, these two are worth considering as auxiliaries to improve soil biological fertility in crop fields. Their use with the goal of selecting appropriate crop mycobiota should be relatively easier in organic farms where weeds are better accepted, whereas their use in conventional farms would require raising farmers’ awareness of the benefits of weeds for soil fertility.

The “full species list” fallacy in Floristic Quality Assessment

AbstractBiological assessments typically involve field surveys that are time‐consuming and require taxonomic expertise. Floristic Quality Assessment, a popular bioassessment method for wetlands and other ecosystems, generally assumes a comprehensive or representative species list for accurate implementation. We explored this long‐held belief by analyzing an essential floristic quality metric (mean conservatism) across real and simulated gradients of species representation in two disparate case studies. In one study, we incrementally removed species at random from an exhaustive floristic survey of a suburban wetland complex in northeast Ohio. Bootstrapping mean conservatism at each removal step, precision scarcely decreased with 10%–30% species loss, becoming noticeable only when about 50% or fewer species remained. For the other study, we exploited varying percentages of dominant species available from hundreds of single‐visit wetland determination surveys throughout Illinois. Comparing dominants‐only mean conservatism with total species mean conservatism, the relationship steadily improved as dominants covered progressively larger fractions of native richness, ranging from r2 = 0.12 at ≤10% dominants to 0.74 at >40% dominants. Both exercises suggest that community size is more important than taxonomic representation or inventory completeness per se in determining accuracy. Our results indicate that full or representative checklists are not a prerequisite for reliable Floristic Quality Assessment, supporting the investigation and potential use of taxonomic shortcuts and empowering a wide range of users beyond expert field botanists.

Using biodiversity databases to assess vascular plant diversity in Protected Areas: a case study of Parque Estadual do Forno Grande, Brazil.

The Parque Estadual do Forno Grande is a fully protected area in the southern Espírito Santo State, Brazil. It belongs to the Atlantic Forest domain, with predominantly dense, ombrophilous, seasonal semi-deciduous forests and herbaceous/shrubby vegetation on rock outcrops. The area is recognised as highly important for conservation, designated as a priority biological area for protecting the Atlantic Forest's biodiversity. Although the importance of Protected Areas in conserving the Atlantic Forest biodiversity is unquestionable, it is crucial to understand the floristic patterns within these regions to develop effective conservation strategies. We utilised national online databases to compile species lists containing relevant information about their biodiversity. The updated list of vascular plants recorded in the Parque Estadual do Forno Grande is available in the "Catálogo de Plantas das Unidades de Conservação do Brasil" and it is presented here with further information on richness, endemism and conservation status. The Parque Estadual do Forno Grande harbours 958 species of vascular plants, of which 79.2% are angiosperms, 18.4% are ferns and 2.4% are lycophytes. Amongst these species, 44% are endemic to the Atlantic Forest. There are 58 threatened species, of which six are Critically Endangered, 39 are Endangered and 13 are Vulnerable. Amongst the threatened species, 51 are endemic to the Atlantic Forest. The number of records and the species richness in this area are notably high for Atlantic Forest standards. Our findings suggest that floristic inventories of Brazilian Protected Areas are a key contribution to the general perception of how much we still do not know about our flora. It also highlights the necessity of supporting floristic surveys in poorly-known areas, especially those remaining as forest remnants.

Effects of weed management on soil metagenomic composition in cultivated chickpea fields

Abstract Non-chemical methods, including mechanical and manual weed management are important for improving crop yields and preserving soil microbial diversity. In Tunisia, chickpea cultivation uses a combination of these methods to suppress weeds. This study aimed to evaluate the impact of weeding practices on chickpea (Cicer arietinum L. cultivar REBHA) yield and soil microbial diversity. Field experiments were conducted at the Technical Center for Biological Agriculture in Essaida, Tunisia, using six plots with manual and no weeding treatments. Chickpea yield was measured, and soil samples were collected for metagenomic analysis. Floristic surveys identified 13 weed species, with Chenopodium album L., Rumex acetosa L., and Urtica dioica L. being the most dominant. Seed yield ranged from 23.2 to 26.2 qls/ha in non-weeded plots and from 25.1 to 30.1 qls/ha in weeded plots, showing an increase in yield (11.75%) with manual weeding.
Soil metagenomics revealed changes in bacterial community composition between the two treatments. The dominant phylum was Pseudomonadota, whose relative abundance increased from 24.88% in non-weeded plots to 34.76% in weeded plots. Alpha diversity indices showed lower species richness and diversity in weeded soils, with 62000 OTUs in weeded plots compared to 43202 OTUs in non-weeded plots. Betaproteobacteria and Alphaproteobacteria exhibited higher OTU counts in weeded soils. The Simpson diversity index was lower in non-weeded soils (0.025) than in weeded soils (0.075), indicating a more irregular microbial distribution in non-weeded plots. Thus, manual weeding improved chickpea yield and altered the soil bacterial community, increasing diversity in key microbial taxa. This study highlights the complex interaction between weed management practices and soil microbial ecosystems, which may influence crop productivity.

Automating field‐based floral surveys with machine learning

Abstract The abundance and diversity of flowering plant species are important indicators of pollinator habitat quality, but traditional field‐based surveying techniques are time‐intensive. Therefore, they are often biased due to under‐sampling and are difficult to scale. Aerial photography was collected across 10 sites located in and around Rouge National Urban Park, Toronto, Canada using a consumer‐grade drone. A convolutional neural network (CNN) was trained to semantically segment, or identify and categorize, pixel clusters which represent flowers in the collected aerial imagery. Specifically, flowers of the dominant taxa found in the depauperate fall flowering plant community were surveyed. This included yellow flowering Solidago spp., white Symphyotrichum ericoides/lanceolatum and purple Symphyotrichum novae‐angliae. The CNN was trained using 930 m2 of manually annotated data, ~1% of the mapped landscape. The trained CNN was tested on 20% of the manually annotated data concealed during training. In addition, it was externally validated by comparing the predicted drone‐derived floral abundance metrics (i.e. floral area (m2) and the number of floral patches) to the field‐based count of floral units estimated for 34 4 m2 plots. The CNN returned accurate multiclassification when evaluated against the testing data. It obtained a precision score of 0.769, a recall of 0.849, and an F1 score of 0.807. The automated floral abundance counting yielded estimates that were strongly correlated with field‐based manual counting. In addition, flower segmentation using the trained CNN was time‐efficient. On average, it took roughly the same amount of time to segment the flowers occurring in an entire drone scene as it took to complete the abundance count of a single quadrat. However, the training process, particularly manual data annotation, was the most time‐consuming component of the study. Practical implication: Overall, the analysis provided valuable insights into automated flower classification and abundance estimation using drone imagery and machine learning. The results demonstrate that these tools can be used to provide accurate and scalable estimates of pollinator habitat quality. Further research should consider diverse wildflower systems to develop the generalizability of the methods.

<i>Parnassia kotzebuei</i> (Parnassiaceae) at the western limit of its distribution

During a floristic survey of the territory of the mountain site "Sokol" of the Ust-Lensky Reserve, new localities of Parnassia kotzebuei were found at the western limit of its range, in the lower reaches of the Lena River. Previously, the limit of this species range was believed to be in the lower reaches of the Indigirka River. Parnassia kotzebuei is included in the list of rare and specially protected plants of the Republic of Sakha (Yakutia), is listed in the regional Red Data Books, and its presence on the territory of the Ust-Lensky Reserve will serve as an additional factor in ensuring the preservation of its populations. Hypotheses of the species appearance in this territory and its distribution in the adjacent area are expressed. Two possible versions are assumed: the relict (Pleistocene) origin of the populations, and the introduction of seeds by waterfowl beards. In both cases, the probability of its hydrochoric dissemination in the vicinity is allowed.

Conservation of wild food plants from wood uses: evidence supporting the protection hypothesis in Northeastern Brazil

BackgroundThe interplay between different uses of woody plants remains underexplored, obscuring our understanding of how a plant’s value for one purpose might shield it from other, more harmful uses. This study examines the protection hypothesis by determining if food uses can protect woody plants (trees and shrubs) from wood uses. We approached the hypothesis from two distinct possibilities: (1) the protective effect is proportional to the intensity of a species’ use for food purposes, and (2) the protective effect only targets key species for food purposes.MethodsThe research was conducted in a rural community within “Restinga” vegetation in Northeast Brazil. To identify important food species for both consumption and income (key species) and the collection areas where they naturally occur, we conducted participatory workshops. We then carried out a floristic survey in these areas to identify woody species that coexist with the key species. Voucher specimens were used to create a field herbarium, which, along with photographs served as visual stimuli during the checklist interviews. The interviewees used a five-point Likert scale to evaluate the species in terms of perceived wood quality, perceived availability, and use for food and wood purposes. To test our hypothesis, we used Cumulative Link Mixed Models (CLMMs), with the wood use as the response variable, food use, perceived availability and perceived quality as the explanatory variables and the interviewee as a random effect. We performed the same model replacing food use for key species food use (a binary variable that had value 1 when the information concerned a key species with actual food use, and value 0 when the information did not concern a key species or concerned a key species that was not used for food purposes).ResultsConsistent with our hypothesis, we identified a protective effect of food use on wood use. However, this effect is not directly proportional to the species’ food use, but is confined to plants with considerable domestic food importance. Perceived availability and quality emerged as notable predictors for wood uses.ConclusionWe advocate for biocultural conservation strategies that enhance the food value of plants for their safeguarding, coupled with measures for non-edible woody species under higher use-pressure.

Mapping plant communities of the Karoo National Park, South Africa, using Sentinel-2 and topo-morphological data

AimsThis study aimed at classifying, mapping and describing the plant communities of the Karoo National Park using floristic surveys in conjunction with Sentinel-2 and topo-morphological data. Study areaKaroo National Park, Western Cape, South Africa. MethodsThe vegetation of the Karoo National Park was delineated into homogenous physiognomic-physiographic units using Sentinel-2 images. A total of 128 survey plots (100 m2 each) were surveyed within the different homogeneous units during the period 2016 to 2020. Within each survey plot, all rooted species were identified and their cover abundance estimated. Each plot was photographed and its geolocation recorded. The floristic data were captured using the Braun Blanquet Personal Computer suite and exported to the JUICE Software programme. A modified TWINSPAN classification was done to derive a first tabled synopsis of the plant communities. The different plant communities were subsequently classified and described according to their diagnostic and dominant species gleaned from the synoptic table. Species richness was determined by counting the number of different species per plant community while the Shannon–Wiener Index and Rich-Gini–Simpson Index of diversity (D) were used to derive indices of species diversity per plant community. Results12 major communities and two sub-communities that are distinctly linked to various abiotic factors were identified, described and mapped. The higher-lying rocky steep midslopes as well as the valley bottomland areas had the highest diversity and species richness. ConclusionsThis study proves the efficacy of using Sentinel-2 and topo-morphological data in classification, description and mapping vegetation of extensive natural areas. The vegetation map and classification of plant communities provide a baseline to inform management decisions.Taxonomic reference: SA-Plant Checklist-2019–2020, South African National Biodiversity Institute, 2020, Botanical Database of Southern Africa (BODATSA) (http://posa.sanbi.org/) [accessed January 2022].

Plants associated with water towers in the Saharan environment: Floristic diversity and therapeutic virtues

Water in arid regions plays a vital role in the existence and development of living beings. The main aim of this work is to study the diversity and therapeutic properties of plant species that grow in the vicinity of water towers (constructions used to store and distribute drinking water) in the Saharan environment (south-western Algeria). Twenty-two floristic surveys were carried out in five representative sites to study the floristic diversity. The biological and phytogeographical spectra were established, and the therapeutic properties were determined for each plant species inventoried. The results show that the floristic richness is of the order of 41 species belonging to 39 genera and 16 botanical families, the most represented of which are the Asteraceae (19.51%) and Poaceae (17.07%). The biological spectrum showed the existence of 20 species of therophytes, and the phytogeographic spectrum indicated that most of the inventoried plant species belonged to the Saharo-Arabian biogeographic element (31.7%). The leaves (51.22%) are the most used for therapeutic aspects, while decoction is the most commonly used preparation method (60.98%). The plants with the highest importance values are Calotropis procera (27.78%), Phoenix dactylifera (16.67%), Cynodon dactylon and Tamarix gallica (15.56% each). This study shows that the various plant species inventoried offer important therapeutic properties for treating various pathologies.

Rapid Assessment of Floral Diversity in Green Spaces within Mumbai City, MS, India

Forests are one of the most biologically active terrestrial ecosystems. They are known to provide varied ecological services and play an important role in maintaining ecological balance. Especially in a metropolitan city like Mumbai, the last remaining tracts of green help in maintaining the microclimate, enriching the groundwater table, and curbing pollution levels. Despite these valuable services provided by forests, deforestation has become rampant in the present era, causing several environmental problems. Urgent steps are required to identify and protect the remaining green areas to maintain ecological balance and slow the pace of climate change. The present study deals with the identification and assessment of the floral diversity of green spaces within Mumbai city and suburban areas. The floristic survey was carried out from June 2021 to October 2022. A total of 111 plant species from 43 families, encompassing 5 herbs, 14 shrubs, 3 climbers, 1 grass, 1 fern, and 87 angiospermic trees, were identified. Through our report, we attempt to shed light on these important green spaces to ensure that they are not treated as isolated spaces but as spaces that complement our daily lives and well-being.

Ethnobotanical Significance of the Flora of Neora Valley National Park in the District of Darjeeling, West Bengal (India)

Recent floristic survey of Neora Valley National Park in Kalimpong sub-division of Darjeeling district of West Bengal revealed that the park is a rich repository of the ethnobotanically important plant resources that includes species of food, medicine, ornamental, poisonous and other assorted ethnic values. Based on available literature and direct interaction with forest dwellers living in the periphery of the park, altogether 83 medicinal, 59 edible, 18 ornamental, 21 poisonous (irritants and lethal) and 11 plants having fascinating assorted ethnic uses have been recognized. The forest dwellers have their own dictionary of local names for the identification of these valuable plant resources by which they are maintaining their own ancient ethnoscience. Many of these medicinal plant species were assessed to be the valuable resources for the treatment of a range of human ailments for which there are no complete answers in the modern medical science (?).

Additions to the Papilionaceous Flora of Pachmarhi Hills, Madhya Pradesh

Thirty nine species of Papilionaceae are reported for the first time from Pachmarhi in Madhya Pradesh. This makes a total record of 99 species (103 taxa) of Papilionaceous plants from the region. As many as 9 species, namely, Argyrolobium flactidum, Atylosia kulnensis, Desmodium floribundum D. maculatum, Flemingia fruticulosa Phasealus velutinus, Shuteria, vestita, Sophora mollis and Teramnus mollis are new records for Madhya Pradesh. This fully justifies the need for intensive floristic surveys of the restricted regions for compilation of Flora of India.

Observations on the Ethnobotany of Madhya Pradesh

Ethnobotanical observations were recorded during the floristic survey carried out in Madhya Pradesh for a period of over seven years. The interesting folk-uses, covering eighty eight species of vascular plants which are not recorded in the important published literature, are presented in the paper.

Studies on the Vascular Flora of Mahendragiri Hill and the Surrounding Regions, Kanyakumari and Tirunelveli Districts, Tamil Nadu

The vascular flora of Mahendragiri hill and the surrounding areas of Kanyakumari and Tirunelveli Districts, Tamil Nadu, is dealt with in this paper. The floristic survey and investigations of the area reveal a varied and heterogeneous vegetetion. Tne vegetation varies from Southern tropical wet-evergreen, moist and dry deciduous to grassland and thorn forests. In all five exploration visits in different seasons were conducted which resulted in a collection of 831 field numbers distributed over 120 families, 398 genera and 579 species. About 21 species endemic to Tirunelveli and Travancore hill%, have been collected from this area. Of the families, Leguminosae, Gramineae, Euphorbiaceae, Rubiaceae, Acanthaceae, Labiatae, Orchidaceae, Arteraceae, Cyperaceze, Moraceae and Asclepizdaceae are in order of dominance. Pteridophytes are represented by 8 families, 14 genera and 16 species, the family Pteridaceae being dominant in having 6 genera and 7 species.

Plant diversity on the edge: floristics, phytogeography, fire responses, and plant conservation of Two Peoples Bay Nature Reserve in the context of OCBIL theory

Context There have been few long-term studies of the flora, phenology, and ecology of specific reserves in the species-rich flora of the Southwest Australian Floristic Region. Aims This project, extending over five decades, aimed to develop an authoritative flora list and acquire data on phenology, threatened species, endemism, old and young landscapes, phytogeography, old lineages, and fire responses at Two Peoples Bay Nature Reserve. Methods The study used botanical collection on repeat surveys, herbarium studies, granite outcrop surveys and comparative phytogeographic analyses from maps on the Western Australian Herbarium’s Florabase. Key results Floristic survey recovered 853 taxa, 26% of those known in the Albany local government area. Possibly as many as 950–1000 taxa will be found in the future. The herbarium collections are the second largest of any conservation reserve in the Albany area. Flowering was most evident in spring and least in autumn. Three declared rare species and 20 conservation priority species were identified, as were short-range endemics, old clades, and natural hybrids. Conclusions The flora is dominated by species predominantly from wetter forest regions. Consequently, Two Peoples Bay Nature Reserve is correctly placed within the Bibbulmun Botanical Province. Several hypotheses of OCBIL theory (which addresses old, climatically-buffered, infertile landscapes) were supported, with increased local endemism, ancient clades, and reduced rates of natural hybridisation identified for the granite inselberg OCBIL Maardjitup Gurlin/Mt Gardner. Implications Long term studies are invaluable for plant inventory. Continuing the minimal use of prescribed burning is advocated from plant data, in support of approaches to help conserve threatened animals.

A taxonomic survey of pteridophytic flora of Lalmai Pahar and Mainamati of Cumilla district, Bangladesh

This study aims to investigate the pteridophytic flora of the Lalmai Pahar, Mainamati, located at the Cumilla district of Bangladesh. Despite the absence of extensive pteridophyte surveys in this hilly forest, it is known to harbour a diverse range of pteridophyte species. This study presents a survey of the pteridophyte diversity within the specified area, documenting a total of 27 taxa. The voucher specimens have been deposited at the Salar Khan Herbarium, Department of Botany at the University of Dhaka. Dhaka Univ. J. Biol. Sci. 33(1): 1-21, 2024 (January)

Environmental DNA metabarcoding of pan trap water to monitor arthropod‐plant interactions

AbstractGlobally, the diversity of arthropods and the plants upon which they rely are under increasing pressure due to a combination of biotic and abiotic anthropogenic stressors. Unfortunately, conventional survey methods used to monitor ecosystems are often challenging to conduct on large scales. Pan traps are a commonly used pollinator survey method and environmental DNA (eDNA) metabarcoding of pan trap water may offer a high‐throughput alternative to aid in the detection of both arthropods and the plant resources they rely on. Here, we examined if eDNA metabarcoding can be used to identify arthropods and plant species from pan trap water, and investigated the effect of different DNA extraction methods. We then compared plant species identified by metabarcoding with observation‐based floral surveys and also assessed the contribution of airborne plant DNA (plant DNA not carried by arthropods) using marble traps to reduce putative false positives in the pan trap dataset. Arthropod eDNA was only detected in 17% of pan trap samples and there was minimal overlap between the eDNA results and morphological identifications. In contrast, for plants, we detected 64 taxa, of which 53 were unique to the eDNA dataset, and no differences were identified between the two extraction kits. We were able to significantly reduce the contribution of airborne plant DNA to the final dataset using marble traps. This study demonstrates that eDNA metabarcoding of pan trap water can detect plant resources used by arthropods and highlights the potential for eDNA metabarcoding to be applied to investigations of arthropod‐plant interactions.

Standardising bee sampling: A systematic review of pan trapping and associated floral surveys.

The use of coloured pan traps (bee bowls, Moericke traps) for sampling bees (and other pollinators) has continuously increased over the last two decades. Although a number of methodological studies and conceptual frameworks offer guidance on standardised sampling, pan trap setups vary widely in characteristics even when optimised for capturing bees. Moreover, some uncertainty persists as to how local flower abundance and diversity influence sampling. We systematically reviewed peer-reviewed studies that used pan traps for bee collection and that were listed in the Web of Science core collection. To gauge methodological variation, we identified a set of relevant methodological criteria and assessed the studies accordingly. For obtaining evidence that pan trap samples and floral environment around traps are correlated, we screened the relevant studies for such correlations. While some aspects of pan trapping (e.g., trap coloration and elevation) were similar in the majority of studies, other aspects varied considerably (e.g., trap volume/diameter and sampling duration). Few studies used floral abundance and/or diversity as an explanatory variable in their analyses of bee samples. Among these studies, we found a considerable variation in key aspects of floral survey methods, such as time and space between vegetation surveys and pan trap sampling, abundance measures (quantitative, semi-quantitative and presence-absence), and processing of raw data prior to analysis. Often studies did not find any correlation between the floral environment and bee samples. Reported correlations varied markedly across studies, even within groups of studies applying a similar method or analysing a similar group of bees. Our synthesis helps to identify key issues of further standardisation of pan trap methodology and of associated floral surveys. In addition to the few aspects that have been standardised over the past decades, we suggest methodological direction for future research using pan traps as a better standardised method for the collection of wild bees. We encourage further studies to illuminate if and how varying floral resources around traps bias bee samples from pan traps. More generally, our synthesis shows that trapping methodologies should be reviewed regularly when their use increases to ensure standardisation.

Prospects and peculiarities of the existence of the genus Oenothera L. species outside the places of cultivation in Odesa region

Cultivated plant species can often become elements of the spontaneous flora fraction of ecotopes of varying degrees of anthropogenic transformation. An example is representatives of the genus Oenothera. Some of them are able to master extreme man-made ecotopes or take root in natural multi-component coenoses. Work with the species of the genus is complicated, since the issues of their hybridization require additional scientific developments. The species Oenothera biennis and O. rubricaulis are the most common spontaneous elements of the flora on the territory of Ukraine. At the same time, the following species were noted for several regions of the northern and central (sometimes–eastern) parts of Ukraine: O. depressa, O. erythrosepala, O. glazioviana, O. hoelscheri, O. villosa. Some species have a limited distribution: O. missuriensis, O. parviflora, O. pycnocarpa, O. fallax, O. speciosa, O. tetragona, O. wienii. According to modern literary data, information on the distribution of Oenothera species for the Odesa region is either absent or critically scarce. In the herbarium collections of the Odesa National University named after I.I. Mechnikov (MSUD), historical and modern, there are some specimens of representatives of this genus. However, all of them relate mainly to the distribution of Oenothera biennis within the borders of Ukraine, or, occasionally, the adjacent territories. The herbarium also contains specimens of other species, but their identification requires the attention of specialists. Literary summaries which contain information about the species of the Oenothera genus (Oenothera biennis) mainly characterize the centuries-old flora of the Odesa region. In general, there are very few cases of spontaneous growth of Oenothera species found in the South of Ukraine. During the floristic survey of ecotopes below the Dniester–Tyligul interfluve, we found several habitats of Oenothera glazioviana. The ecological conditions of the sites differed significantly. In general, most species of the genus Oenothera do not have a high invasive potential, however, monitoring the establishment of species of the genus in ecotopes with different anthropogenic load is advisablе.