Floristic Composition Research Articles

Changes in blood physiological and biochemical parameters and intestinal flora in newborn horses and mares with angular limb deformities

IntroductionAngular limb deformities (ALDs) are a common skeletal development disorder in newborn foals. This condition affects the growth and development of foals and severely impacts their future athletic performance and economic value, causing significant financial losses to the horse industry. Placentitis, metritis, and severe metabolic diseases during mare pregnancy are significant causes of ALDs in newborn foals. It has been established that intestinal flora disorders can easily lead to inflammatory and metabolic diseases in the host. However, the incidence of ALDs in foals in Zhaosu County, Xinjiang, a key production area of China's horse industry, remains unclear. Additionally, the relationship between functional changes in foals with ALDs and their mares and changes in their intestinal flora is not well-understood.MethodsThis study investigated the status of ALD in newborn foals through clinical observation and imaging examinations. In addition, molecular biological methods were applied to examine the effects of ALDs foals and their mares on physiological and biochemical markers and gut microbiota.ResultsThe results showed that the incidence of ALD in Zhaosu area of China was 4.13%. In addition, by comparing and correlating the physiological and biochemical indicators and intestinal flora of foals and mares with ALD with those of healthy horses, it was found that foals and mares with ALD may promote the occurrence and development of the disease through the “blood marker changes-intestinal flora-ALDs” axis. In addition, by comparing the physiological and biochemical indicators and intestinal flora of foals and mares with ALD with the intestinal flora of healthy horses, it was found that the physiological and biochemical indicators and intestinal flora structure and metabolic pathways of foals and mares with ALD had significant changes.DiscussionThe diversity, species composition, and function of the intestinal flora of ALDs and their mares were significantly altered. These findings provide a scientific basis for understanding the etiology of ALDs in foals and offer new perspectives for diagnosing and treatment ALDs in newborn foals.

The Natural Vegetation of Residual Wetlands in the Hinterland of Western Sicily (Italy)

An overview of the wetland vegetation of the hinterland of western Sicily, between the hills located south of the Palermo Mts. and the Sicani Mts., is presented herein. This study was conducted according to Braun-Blanquet’s phytosociological method, through a survey carried out mainly within six important biotopes: (1) Gorgo Lungo (“Bosco Ficuzza”; municipality of Godrano); (2) Gorgo Marosa (on the southern side of Rocca Busambra; municipality of Godrano); (3) Gorgo di Piano Scala (on the northern side of Mt. Cardellia; municipality of Corleone); (4) and (5) Gorgo Carcaci and Gorgo Carcaciotto (both on the south-eastern slope of Mt. Carcaci; municipality of Castronovo di Sicilia); and (6) Gorgo S. Andrea (municipality of Castronovo di Sicilia). A vegetation analysis was carried out on the basis of 107 field relevés, together with other data taken from scientific literature. A total of 28 plant communities were identified, between hydrophytics of the classes Lemnetea minoris (3 associations) and Potamogetonetea pectinati (6 associations), helophytics of the Phragmito-Magnocaricetea class (14 associations and 1 community), ephemeral of the Isöeto-Nanojuncetea class (2 communities) and perennial herbaceous vegetation of the Molinio-Arrhenatheretea class (1 association and 1 community). A new syntaxon is also described (Callitricho obtusangulae-Glycerietum notatae ass. nova), as an endemic association of the hinterland of western Sicily, referred to as the Alopecuro-Glycerion spicatae alliance. For all surveyed communities, new insights into syntaxonomy and diagnostic taxa are provided, as well as for the floristic composition, synecology, syndynamism and synchorology of the aquatic vegetation of western Sicily.

Habitats, Plant Diversity, and Molecular Phylogeny of Endemic Relic Species Incarvillea semiretschenskia (Bignoniaceae)

Incarvillea semiretschenskia (B. Fedtsch.) Grierson is listed in the Red Data Book of Kazakhstan as a rare relic, narrowly endemic species of the Shu-Ile low mountains (Kazakhstan). The aim of this research was to advance our knowledge of the ecological conditions of its habitats, the floristic composition of plant communities, and molecular phylogeny, as well as to identify threats to the species’ existence. The ecological conditions of I. semiretschenskia habitats are rocky slopes and intermountain valleys of the low mountains in the altitude range from 812 to 1075 m asl with light chestnut mountain soils of little development, having a light granulometric composition and containing insignificant amounts of organic matter. We revealed 164 species of vascular plants in the communities of I. semiretschenskia, including five endemics and five species from the Red Data Book of Kazakhstan. Anthropogenic factors associated with grazing, fires, and limestone mining were identified as leading to a decrease in the number and density of populations. New insights into the relationship and time of diversification in the genus Incarvillea are gained through the use of several accessions of I. semiretschenskia and I. potaninii Batalin in the phylogenetic study. Preliminary fingerprint analysis shows relatively high genetic variability within populations of I. semiretschenskia. This unique relic species has survived since the Miocene epoch and exists to this day only in the Shu-Ile low mountains. To preserve this rare species, measures are proposed to create plant micro-reserves to provide ex situ collections and ensure future in situ restoration efforts.

Effects of Saccharomyces boulardii on microbiota composition and metabolite levels in the small intestine of constipated mice

Saccharomyces boulardii (S. boulardii) is a fungal probiotic used to treat digestive disorders. However, the mechanism(s) by which S. boulardii affects the small intestine remains unclear. Here, we aimed to explore the effects of S. boulardii on the small intestine and the underlying mechanisms in mice with loperamide-induced constipation. While S. boulardii administration did not fully reverse the alterations in loperamide-induced defecation parameters, it altered the small intestinal floral composition toward a community conducive to alleviate constipation. Moreover, S. boulardii up-regulated the expression of tyrosine-protein kinase Kit (c-Kit), aquaporin 3 (AQP3), interleukin (IL)-10, myosin light chain kinase (MLCK), and phosphorylated myosin light chain 20 (P-MLC20), while concurrently down-regulating the expression levels of inducible nitric oxide synthase (iNOS), p65, and IL-17 A. These alterations indicate a discernible effect of small intestinal water reabsorption, inflammatory factor levels, and smooth muscle contraction. Saccharomyces boulardii also positively regulated small intestinal metabolite levels, such as fructose 6-phosphate, dihomo-alpha-linolenic acid, and 3-(4-hydroxyphenyl) lactate, and participated in metabolic pathways such as arginine biosynthesis, linoleic acid metabolism, and protein digestion and absorption. While not fully reversing defecation changes, Saccharomyces boulardii alters intestinal flora, up-regulates key proteins affecting water reabsorption and inflammation, and positively influences metabolic pathways. Our study provides serves as a basis for further studies on the application of S. boulardii in the treatment of intestinal disorders.

Eco-Habitat Assessment of One of the Most Expensive Edible Mushrooms (Tricholoma matsutake) in Genekha, Thimphu, Bhutan

Tricholoma matsutake (S. Ito & Imai) Singer is most expensive edible mushroom, naturally grown dispersed in temperate oak pine forested areas in Bhutan. It is ectomycorrhizal fungi with high ecological and economic value requiring an important ecological niche and symbiotic tree associates. The present study is an attempt to give an account on floristic composition and vegetation structure of Tricholoma matsutake habitat in Genekha, Thimphu. A total of 10 plots were enumerated with plot size of 20m X 20m, 5m X 5m and 2m X 2m for trees, shrubs and herbs respectively. PAST (Paleontological Statistics) 4.10 software was used for diversity analysis. A total of 58 species under 44 genera belonging to 26 families were recorded in the natural habitat of matsutake. The study revealed that T. matsutake is associated with Quercus semecarpifolia Sm., Pinus wallichiana A.B. Jack., Rhododendron spp., and Pieris formosa (Wall.) D. Don. J-shaped distribution curve of DBH and height class with fair regeneration status is obtained for associated species in the habitat. The Menhinick’s species richness of (2.26, 2) for both North facing habitat (NFH) and South facing habitats (SFH) indicated high species richness. Moderate and low diversity is indicated with Simpsons index 0.94 (NFH) and 0.76 (SFH), Shannon index 3.06 (NFH) and 2.49 (SFH) with moderately even distribution 0.74(NFH) and 0.73 (SFH) of species in the habitats. Sorensens similarity index of 0.9 indicated highly similar species composition between two habitats. This research provides current floristic and vegetation structure of Tricholoma matsutake habitat, that will have high significance in habitat management and conservation.

The Impact of the Interaction between Intestinal Flora and Intestinal Wall Immune Microenvironment on Ulcerative Colitis

Ulcerative colitis (UC) is a chronic, non-specific inflammatory disease of the intestine with an unknown etiology. The primary clinical manifestations include recurrent abdominal pain, diarrhea, mucus and purulent discharge, bloody stools, and tenesmus. Experts generally agree that the onset of this disease is primarily associated with factors such as intestinal flora, dietary composition, genetics, immunity, infections, and systemic inflammation. The intestinal flora constitutes the largest microecological system within the human body, consisting of a vast array of microorganisms that interact dynamically with the immune cells located in the intestinal wall. This interaction is crucial for maintaining a balance with the intestinal mucosa. The intestinal flora plays a significant role in the development of the immune system, sustaining normal immune function, and cooperatively countering the invasion of pathogenic bacteria. Alterations in the composition of the intestinal flora can influence the equilibrium between intestinal tolerance and immunity. Research has demonstrated that the disease process in UC patients is closely linked to disturbances in the structure of the intestinal bacterial flora，and symptoms can be significantly alleviated following the transplantation of normal flora. Therefore,Modulating the structure of the intestinal microbiota may serve as an effective strategy for treating ulcerative colitis (UC). This article examines the relationship between intestinal flora and intestinal wall immunity in the context of UC, while also exploring novel approaches for its treatment.

Pulmonary Flora-Derived Lipopolysaccharide Mediates Lung-Brain Axis through Activating Microglia Involved in Polystyrene Microplastic-Induced Cognitive Dysfunction.

Microplastics (MPs) have been detected in the atmospheric and the human respiratory system, indicating that the respiratory tract is a significant exposure route for MPs. However, the effect of inhaled MPs on cognitive function has not been adequately studied. Here, a C57BL/6 J mouse model of inhalation exposure to polystyrene MPs (PS-MPs, 5 µm, 60 d) is established by intratracheal instillation. Interestingly, in vivo fluorescence imaging and transmission electron microscopy reveal that PS-MPs do not accumulate in the brain. However, behavioral experiments shows that cognitive function of mice is impaired, accompanied by histopathological damage of lung and brain tissue. Transcriptomic studies in hippocampal and lung tissue have demonstrated key neuroplasticity factors as well as cognitive deficits linked to lung injury, respectively. Mechanistically, the lung-brain axis plays a central role in PS-MPs-induced neurological damage, as demonstrated by pulmonary flora transplantation, lipopolysaccharide (LPS) intervention, and cell co-culture experiments. Together, inhalation of PS-MPs reduces cognitive function by altering the composition of pulmonary flora to produce more LPS and promoting M1 polarization of microglia, which provides new insights into the mechanism of nerve damage caused by inhaled MPs and also sheds new light on the prevention of neurotoxicity of environmental pollutants.

Relicts of Carrasco in the Tocantins Cerrado: Diversity and Floristic Relationships

Objective: To describe the floristic and structural diversity of the Carrasco in the municipality of Porto Nacional. Theoretical Framework: This study is based on analyzing the floristic diversity, vegetation structure, and floristic similarity to verify its classification as a phytophysiognomy associated with the Cerrado of Tocantins. Method: Fifty plots measuring 10 x 10 meters were allocated. Sampling criteria included circumference ≥ 10 cm at ground level and height ≥ 1 m. The phytosociological data were analyzed using the FITOPAC software. Results and Discussion: A total of 4,110 individuals were found, distributed among 59 species from 37 genera and 22 botanical families. The vegetation showed homogeneity and diversity comparable to other Cerrado areas but with distinct characteristics, such as the presence of lianas and low ecological dominance. The floristic similarity analysis revealed a low similarity between the "Carrasco" and other Cerrado phytophysiognomies, highlighting the uniqueness of this vegetation. Research Implications: The 'Carrasco' possesses unique floristic, structural, and similarity characteristics, being a vegetational relict that justifies its designation as a new phytophysiognomy of Tocantins. Originality/Value: This study contributes to the literature by providing a detailed analysis of the floristic composition of the “Carrasco” phytophysiognomy, a new xerophytic and deciduous phytophysiognomy on sandy soils in the state of Tocantins, highlighting its importance for biodiversity conservation within the Cerrado biome. The relevance of this research is to expand the understanding of the ecological characteristics of this relic, offering essential insights for future conservation and management actions.

Atlantic Forest Regeneration Dynamics Following Human Disturbance Cessation in Brazil

The Brazilian Atlantic Forest (BAF) is one of the most important biodiversity hotspots and species-rich ecosystems globally. Due to human activities, it has been significantly reduced and fragmented. This study examined both biotic (floristic composition, diversity, and structure) and abiotic (topographic and soil) factors in BAF fragments undergoing varying levels and durations of human disturbance cessation: approximately 20 years (20 y), ~30 years (30 y), and over 40 years (>40 y). We aimed to understand the recovery dynamics of floristic composition, diversity, and structure in BAF fragments in relation to abiotic factors. Several statistical tools were employed to examine similarities/differences and relationships. Forests of the 30 y group exhibit significantly greater homogeneity in terms of floristic composition, while forests of the 20 y group are characterized by lower species abundance and diversity. The floristic composition was primarily influenced by soil features and the time of disturbance. Under “Environmental Protection Areas”, soil–vegetation recovery can occur more swiftly than usually observed for BAF. A significant BAF recovery was observed approximately 40 years after the end of human disturbance. A partial recovery featured 30 y disturbed areas, while in 20 y forests, recovery is in its early stages. Human-disturbed BAF can gradually rebound when effective management practices are implemented.

Floristic composition, biomass and carbon stock of grasslands along elevation gradient in Indian central Himalaya

Floristic composition, biomass and carbon stock of grasslands along elevation gradient in Indian central Himalaya

Isolation and Identification of Individual Intestinal Bacteria Associated with Waist Circumference Increase

Abstract Background: The bacterial flora of the gastrointestinal tract (GIT) of humans, there are differences in the composition of the flora which are influenced by age, diet, cultural conditions, and the use of antibiotics. Since the origin of obesity is multifactorial and complex, resulting from the interaction between genetic/epigenetic, environmental, and socio-psychological determinants, in addition to the bacterial composition of the intestinal flora could be a third element involved in the development of excess weight. Therefore intestinal flora plays an intermediary role in the metabolism and inflammation and is involved in the pathogenesis of diabetes type 2 and obesity. Objectives: This study aimed to draw the relationship between identified intestinal bacterial types on increasing the waist circumference among study participants. Materials and Methods: This study included the collection of 180 samples from participants (20–60 years) during the period from December 2022 to June 2023. Body mass index and waist circumference were calculated for study participants and intestinal bacteria was microbiologically and biochemically identified. Results: Results obtained showed that the intestinal flora of obese participants is significantly (P < 0.001) different from that of thin and normal participants. This flora was characterized by the presence of high levels of Gram’s negative at the percentage (80.6% in obese), and Gram’s positive at the percentage (19.4% in obese), with higher body mass index and waist circumference than those participants, with lower body mass index and waist circumference. Conclusion: This study showed a significant relationship between intestinal bacteria and diabetic patients with increased body mass index and waist circumference, leading to probable useful tools for developing strategies to prevent obesity.

Exploring gut microbiota diurnal fluctuation in alcohol-dependent patients with sleep disturbance.

Introduction. Alcohol dependence (AD) and sleep disturbance (SD) independently affect gut microbiota, potentially disrupting the circadian rhythm of the microbiota and the host. However, the impact of SD on the composition and rhythmicity of gut flora in AD patients remains poorly understood.Gap Statement. Characteristics of gut flora and diurnal oscillations in AD patients experiencing SD are unknown.Aim. This study aims to explore alterations in gut flora and diurnal oscillations in AD patients experiencing SD.Methodology. Thirty-two AD patients and 20 healthy subjects participated, providing faecal samples at 7 : 00 AM, 11 : 00 AM, 3 : 00 PM and 7 : 00 PM for gut microbiota analysis using 16S rDNA sequencing. AD patients were further categorized into those with poor sleep (ADwPS) and those with good sleep (ADwGS) for further analyses.Results. The ADwPS group demonstrated elevated levels of anxiety, depression and withdrawal severity compared to the ADwGS group (all P<0.05). The β-diversity of gut microbiota in the ADwPS group differed from that in the ADwGS group (P<0.05). Bacterial abundances at various taxonomic levels, including Cyanobacteria and Pseudomonadales, differed between the ADwPS and ADwGS groups (all P<0.05). Utilizing unweighted UniFrac analysis, the β-diversity of gut microbiota in the ADwPS group demonstrated robust diurnal oscillation (P<0.05), whereas this pattern was statistically insignificant in the ADwGS group. Notably, the abundance of pathogenic bacteria like Pseudomonadales and Pseudomonadaceae exhibited marked diurnal fluctuation in the ADwPS group (all P<0.05).Conclusion. SD in AD patients extends beyond alcohol-induced alterations, impacting gut microbiota composition, function and diurnal oscillation patterns. This highlights its add-on influence, supplementing AD-related changes.

Characteristics of polysacchadride from Psoralea corylifolia in simulated gastrointestinal digestion and microbial fermentation

Psoralea corylifolia polysaccharide (PPs) was the bioactive ingredient that conferred health benefits. In this study, simulated digestion and anaerobic fermentation models were employed to explore the digestion and fermentation characteristics of PPs and its effect on the intestinal microbiota. The results revealed that while the total and reducing sugar contents of PPs increased after simulated digestion, their molecular weight, monosaccharide composition, and overall structure remained unchanged, indicating that PPs was not degraded during digestion. On the other hand, during anaerobic fermentation, PPs was metabolized by intestinal microbiota, leading to the production of acetic acid and propionic acid, which subsequently reduced the pH of the fecal culture solution. Additionally, PPs modulated the composition of the intestinal flora, significantly decreasing the relative abundance of Proteobacteria while increasing the relative abundance of Bacteroidota and Firmicutes to varying degrees. This study highlighted the potential of PPs for development as functional foods and therapeutic agents.

Vegetation community recovery on restored bottomland hardwood forests in northeast Indiana, USA.

Vegetation communities in restored bottomland hardwood forests in northeast Indiana were studied 6-21 years after restoration to assess progress toward restoration objectives. The study focused on four sites that were restored to compensate for resource injuries after contaminant releases. The restored sites were compared with four reference-site conditions, including crops (prerestoration condition), old field communities representing a no-management alternative, locally sampled second-growth mature forests, and forest community types described by the US National Vegetation Classification (USNVC), which represent ideal or defining conditions of recognized vegetation communities. Fixed-area plots provided data on field-sampled environmental variables, vegetation, soil, and hydrological conditions for crops, old fields, restored areas, and mature forests. The USNVC database provided quantitative data for three historically and geographically relevant reference forest community types for comparison with the sampled communities. Results of nonmetric multidimensional scaling based on species cover revealed clear gradients relating to site age and canopy development. Along those gradients, restored areas demonstrated increasing similarity to mature forest reference communities in terms of floristic composition. Specifically, the floristic quality of restored areas was significantly greater than that of crops and old fields. Furthermore, soil health measurements of physical, chemical, and hydrological conditions indicated significant improvements in restored site soils compared with prerestoration conditions represented by cropland soils. Descriptions and data from the USNVC provided ecological context for restoration target conditions and facilitated the assessment of restoration recovery along a trajectory from starting conditions to those target conditions. Descriptions by USNVC also helped identify deviations from the intended restoration objectives (e.g., invasive species recruitment) and potential adaptive management actions to return sites to their intended trajectories. Integr Environ Assess Manag 2024;20:1917-1938. Published 2024. This article is a U.S. Government work and is in the public domain in the USA. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).

Using floristic data to shed light on the origin of flora in the poorly studied common cypress habitats of Northern Iran

Despite the ecological importance, there is a large knowledge gap on different habitat types of the Hyrcanian World Heritage. The Hyrcanian Common Cypress Habitats (HCCHs), the easternmost part of the Common Cypress habitats in the world, are one of these habitat types. HCCHs are understudied and threatened by a variety of anthropogenic disturbances. In the current study, synthesizing the floristic data of all HCCHs, we compared the species composition of HCCHs to 20 habitats from the Hyrcanian, Irano-Turanian, and Mediterranean regions. Also, we candidate the HCCH with the highest conservation priority by calculating the phylogenetic richness of HCCHs. The ordination analysis results showed that the HCCHs form a distinct group from the three regions. Thus, we found that the HCCHs have a unique floristic composition. The geographical distance had a weak effect on the grouping (Procrustes Sum of Squares = 0.886 and p-value < 0.001). We also detected that the phylogenetic richness of the HCCHs is highest in the Golestan National Park which is the easternmost habitat of HCCHs. Therefore, this park had the most conservation value. Our results provide new insights into the floristic composition and conservation status of the Hyrcanian World Heritage. We conclude that the bioclimatic classifications might be a better predictor of floristic compositions than biogeographical classifications. Based on the observation of Hyrcanian habitats with intermediate floristic composition, it is necessary to reconsider the biogeographical classifications of the region. Our findings are useful for developing management strategies for HCCHs.

Intestinal microflora and metabolites affect the progression of acute pancreatitis (AP)

Specific intestinal metabolites are closely associated with the classification, severity, and necrosis of acute pancreatitis (AP) and provide novel insights for in-depth clinical investigations. In this study, the gut microbiota and metabolites of 49 AP patients at different treatment stages and severities were analysed via 16S rDNA sequencing and untargeted metabolomics to investigate the trends in gut microbiota composition and metabolome profiles observed in patients with severe AP. These findings revealed an imbalance in intestinal flora homeostasis among AP patients characterized by a decrease in probiotics and an increase in opportunistic pathogens, which leads to damage to the intestinal mucosal barrier through reduced short-chain fatty acid (SCFA) secretion and disruption of the intestinal epithelium. This dysbiosis influences energy metabolism, anti-inflammatory responses, and immune regulation, and these results highlight significant differences in energy metabolism pathways. These findings suggest that the differential composition of intestinal flora, along with alterations in intestinal metabolites and metabolic pathways, contribute to the compromised integrity of the intestinal mucosal barrier and disturbances in energy metabolism in patients with severe AP.

PHYTOSOCIOLOGICAL SURVEY IN PASTURE AREAS IMPLANTED WITH GRASSES OF THE GENUS BRACHIARIA IN DIFFERENT RELIEFS

The agricultural sector is essential to the Brazilian economy, contributing 26% of gross revenue in 2021 and 24.8% in 2022. Brazil, the world's largest beef producer, depends on pastures as the main source of feed for its cattle, with Brachiaria grass playing a significant role. role. Inadequate management of these areas often leads to degradation, characterized by the proliferation of weeds, which require intervention. The study was carried out with the objective of carrying out a phytosociological survey in pasture areas at different altitudes to evaluate the floristic composition and identify the forage potential or harmful characteristics of the species present. Phytosociological surveys were carried out in pastures at IFES – Campus Santa Teresa, ES, in two areas with distinct topographic features (hills and plains). The species were identified and classified as palatable, toxic or unpalatable. Sampling occurred during the dry (winter) and rainy (summer) seasons, and species were quantified according to frequency, density and abundance. A total of 47 species distributed in 14 families were identified. The Fabaceae family was the most prevalent, followed by Amaranthaceae and Malvaceae. Alysicarpus vaginalis (IVI 49.06) was dominant in the hillside area (Area A), while Cyperus rotundus (IVI 115.25) stood out in the plain area (Area B). The similarity index between the areas was 26.42%. The study highlighted the diversity of species in Brachiaria pastures, emphasizing the need for targeted management to control unpalatable and toxic plants and, at the same time, optimize forage potential. The low similarity between the areas suggests distinct ecological dynamics.

The Induction of Diabetes by High-Sugar and High-Fat Diets and Potential Applications of Healthy Eating

The rising prevalence of diabetes has intensified research in this field. Among the various causes of diabetes, insulin resistance plays a crucial role. High-sugar, high-fat diets are known to induce hyperglycemia and obesity, which significantly affect the progression of insulin resistance. It should be noted that obesity can significantly increase insulin resistance, thereby inducing prediabetes and aggravating diabetic symptoms. Additionally, such diets disturb the balance of intestinal flora. Controlling carbohydrate intake through low-carb or ketogenic diets can effectively improve glucose and lipid metabolism, improving insulin sensitivity, reducing insulin resistance, and lowering insulin levels. Adjustments to diet can also help shape a healthy gut flora composition. Furthermore, consuming natural products like flavonoids can also contribute to the improvement of diabetic symptoms. This article summarizes the impact of high-sugar, high-fat diets on the progression of diabetes, their effects on insulin resistance and intestinal flora, and the regulatory benefits of a healthy diet in managing diabetes.

Anti-inflammatory properties of Bacillus pumilus TS1 in lipopolysaccharide-induced inflammatory damage in broilers

This study investigates whether Bacillus pumilus TS1 improves growth performance and alleviates inflammatory damage in broilers and explored its feasibility as an antibiotic alternative. We divided 240 one-day-old AA308 white-finned broilers into five groups (con, LPS, TS1L + LPS, TS1M + LPS and TS1H + LPS). The TS1L + LPS, TS1M + LPS and TS1H + LPS groups were fed TS1 for 15 days by gavage. The LPS, TS1L + LPS, TS1M + LPS and TS1H + LPS groups were injected intraperitoneally with 1 mg/kg LPS for three days. We investigated the probiotic and anti-inflammatory activities by measuring body weight, sequencing the intestinal flora and examining the structure of tissues by using pathological stain, real-time PCR, Western blotting and immunohistochemical detection. TS1 could improve growth performance and intestinal flora composition, also reduced different organ damage and inflammatory cytokine expression in serum and organs. The mechanism may involve upregulating HSP60 and HSP70 expression, targeting and regulating Nrf2 and P38 MAPK and modulating NF-κB and HO-1 expression at the transcriptional level in different organs. B. pumilus TS1 alleviated Inflammatory injury caused by LPS and attenuated the inflammatory response in broilers, and these effects were achieved through MAPK and Nrf2 regulation of HSPs/HO-1 in different organs. The above results suggested broilers fed with TS1 could release the LPS caused organ damage, and the most suggested dosage was 1.4 × 108 CFU/mL.

Comparative analysis of intestinal flora community composition and diversity of Anas platyrhynchos and Anser fabalis in the Yellow River Delta

Abstract The Yellow River Delta wetland is a habitat for wintering waterfowl, where mallard (Anas platyrhynchos) and tundra bean goose (Anser fabalis) are commonly observed. Although the intestinal flora plays a pivotal role in maintaining host health and promoting nutrient absorption, it remains unclear whether the same overwintering habitat and challenging feeding conditions specifically affect the composition and diversity of intestinal flora in both species. Here, high-throughput sequencing was used to compare the intestinal bacterial communities of mallard and tundra bean goose from the same region. There was no significant difference in alpha diversity and beta diversity between the two species. Firmicutes and Proteobacteria were the two predominant phyla, accounting for more than 50.00% of the total. They enhanced the nutrient absorption and metabolic efficiency during overwintering. Catellicoccus was the most dominant genus in the intestinal flora of tundra bean goose, accounting for 33.91%, whereas Clostridium sensu stricto 1 was the most dominant genus in mallard, accounting for 7.34%. This difference is considered to be related to the feeding habits of these two species. No significant differences in the structure and diversity of intestinal bacterial communities were observed between mallard and tundra bean goose, and the similarity of intestinal flora between them was high. This study deepens our understanding of the intestinal flora of two coexisting species with different dietary preferences in the Yellow River Delta. It also lays the foundation for waterfowl disease prevention in this area, carrying significant scientific and practical implications for biodiversity and ecosystem conservation.