The use of Supplementary Cementitious Materials (SCM) such as Fly ash, Granulated Slag and Silica fume as a replacement for Portland cement in concrete presents one viable solution with multiple benefits for the sustainable development of the construction industry. Out of various supplementary materials, Fly ash is the most widely used material worldwide. Fly ash has excellent structural and durability characteristics. The pore filling effect and pozzolanic properties of Flyash improves the properties of fresh and hardened concrete. In addition to the cost saving bythe replacement amount of cement; it reduces co2emission during the manufacture of Portland cement. In the present investigation, a controlled beam and six number of ESP flyash and Pond flyash beams of size 200 x 100 x 1700 mm were cast. This paper deals with the investigation on the flexural behaviour of beam with pond flyash and ESP (Electro static precipitated) flyash at various cement replacement levels of 4%, 6%, and 8% and the results are compared with controlled beam. The flexural test was conducted to determine the ultimate load carrying capacity of the beams and their corresponding mid span deflection, load point deflection, crack pattern, Stress vs strain are measured. The flexural strength of ESP flyash is increased 24.76% for 8% replacement level and pond flyash is increased 10.97 % for 6% replacement level than controlled beam respectively. This study revealed that the replacement of cement by flyash concrete provides higher compressive strength & flexural strength compared with controlled concrete.