Subspecies Of Fleas Research Articles

Genetic Evidence of an Isolation Barrier between Flea Subspecies of Citellophilus tesquorum (Wagner, 1898) (Siphonaptera: Ceratophyllidae).

Simple SummaryWe studied field collections of two flea subspecies that are plague vectors on the vast Palearctic territory. Analysing the molecular–genetic, geographical, morphological, and reproductive isolation criteria, we conclude that these subspecies could be considered different species.This study investigated the relationship between two subspecies of the Citellophilus tesquorum flea, C. t. altaicus and C. t. sungaris, which are vectors of the bacterium Yersinia pestis that causes human plague across the vast territories of the Palearctic. Adult fleas were collected from 16 localities and 11 populations in 2019 and 2020. Specimens were morphologically verified for subspecies status and analysed for mitochondrial cytochrome c oxidase subunit I (COI) DNA, nuclear ribosomal cluster internal transcribed spacer 1 (ITS1) and ITS2, and Wolbachia-infection status. Our results demonstrated a genetic difference between C. t. altaicus and C. t. sungaris. According to mitochondrial data, the genetic distance between clades of C. t. altaicus and C. t. sungaris was comparable with the species divergence of the genus Callopsylla, which is closely related to Citellophilus. All studied populations of C. t. altaicus were Wolbachia-infected, whereas all studied populations of C. t. sungaris were symbiont-free. Data for ITS1 and ITS2 had much lower phylogenetic signals than mitochondrial data; however, diagnostic substitutions for C. t. altaicus and C. t. sungaris delimitation were also revealed. Analysis of a hardly accessible report on cross experiments allowed us to conclude the partial postzygotic isolation between these subspecies. Taken together, the molecular-genetic, geographical, morphological, and reproductive isolation findings verified that C. t. altaicus and C. t. sungaris subspecies could be considered as different species.

Fleas of mammals and patterns of distributional congruence in northwestern Argentina: A preliminary biogeographic analysis

In few groups of parasites have the patterns of distribution been studied using quantitative methods, even though, the study of these organisms indirectly provides information on the biogeographic history of their hosts, and in turn, the history of the hosts allows elucidation of speciation events of the parasites. Our objective was to quantitatively identify distributional congruence patterns of native fleas in northwestern Argentina. We analyzed 159 georeferenced distributional records of 47 species and six subspecies of fleas in northwestern Argentina using NDM/VNDM software. We found eight consensus areas, defined by 17 species and two subspecies, included in six patterns of distributional congruence (PDCs) with endemic and non-endemic fleas. The PDCs with the greatest values of endemicity (E) were mainly associated with Monte and Yungas Forests areas. All patterns indicated strong tendency of the Yungas Forests as a possible endemism area. Our results indicate that distributional congruence centers are generally located in Yungas Forests areas and highlight the importance of these areas in conservation and historical biology. This new information will allow delimitation of areas in the region at a more detailed resolution in the future.

Differentiation of flea communities infesting small mammals across selected habitats of the Baltic coast, central lowlands, and southern mountains of Poland

Only a few studies comparing flea composition on the coast and in the mountains have been conducted. We investigated differences in flea communities infesting small mammals in selected habitats in northern, central, and southern Poland. We predicted (1) a greater number of flea species in the southeastern Poland and a lower number in the north, (2) a greater number of flea species in fertile and wet habitats than in poor and arid habitats, and (3) a low similarity of flea species between flea communities in western and eastern Poland. We found a negative effect of increasing latitude on flea species richness. We suppose that the mountains providing a variety of environments and the limits of the geographic ranges of several flea subspecies in southeastern Poland result in a higher number of flea species. There was a positive effect of increasing wetness of habitat on flea species richness. We found a high diversity in flea species composition between western and eastern Poland (beta diversity = 11) and between central and eastern Poland (beta diversity = 12). Re-colonization of Poland by small mammals and their ectoparasites from different (western and eastern) refugees can affect on this high diversity of flea species.

Plague Microbe Aggregation in the Organism of Fleas (Siphonaptera) with Different Vector Ability

The following factors have been used to characterize 12 species and subspecies of fleas (Siphonaptera) and plague microbe (Yersinia pestis subs. pestis): the proportion of fleas with “blocks”, “blockules” and their ratio, called the index of microbe aggregation. The block formation is registered in 10, whereas formation of blockules are registered in all of the studied species and subspecies of Siphonaptera , the difference between active and passive transmitters according to the last characteristics being unreliable. The fleas, among of which “blocked” ones are not revealed in the experiments, are capable to transmit the causative agent of plague, sometimes with generalization of infectious process in animals, that can provide for continuous transmission of microbe without formation of “blocks” in the proventriculus. Thus, the high value of “rate of block formation” does not reflect the existence of coevolution in the interactions between microbe and its transmitter.

Comparison of the activity of selamectin, imidacloprid and fipronil for the treatment of cats infested experimentally with Ctenocephalides felis felis and Ctenocephalides felis strongylus

Twenty adult, domestic short hair cats were randomly allocated into four groups of five cats and housed in separated cages. Each cat was infested with 25 fleas Ctenocephalides felis felis and 25 Ctenocephalides felis strongylus and 2 days later (day 0) the cats in group 1, 2 and 3 received a spot on application of selamectin, imidacloprid or fipronil, respectively, while the cats in group four were not treated. The cats were combed 48 h later, the fleas were removed, counted and their subspecies were determined. All the cats were reinfested with the same number of the two subspecies of fleas on days 7, 14, 21, 29 and 35. The efficacy of each treatment was calculated 48 h after each infestation. The mean number of fleas on the control cats was 16.4 C. f. felis and 13.4 C. f. strongylus. The three treatments were effective for the first 31 days for C. f. felis and for the full 37 days for C. f. strongylus. Over the first 31 days, the efficacy of selamectin ranged from 89 to 100% and 85 to 100% against C. f. felis and C. f. strongylus, respectively, the efficacy of imidacloprid ranged from 76 to 100% and 92 to 100% and the efficacy of fipronil ranged from 98 to 100% and 97 to 100% against C. f. felis and C. f. strongylus. There were no significant differences between the control of C. f. felis and C. f. strongylus by the three products.

Fleas of Small Mammals in Uruguay, with New Host and Distribution Records

Fleas are ectoparasitic insects of many birds and mammals. More than 200 species and subspecies of fleas have been recorded from South America; however, they have been poorly studied in Uruguay. In the present study, Polygenis (Neopolygenis) atopus, Polygenis (Neopolygenis) massoiai, Polygenis (Polygenis) axius axius, Craneopsylla minerva minerva, and Sternopsylla distincta are reported for the first time in Uruguay. These fleas parasitize wild rodents, marsupials, and bats, and most of the ectoparasite–host associations are recorded for the first time. Results obtained are consistent with findings in nearby Argentinean and Brazilian localities.

New records of rodent fleas from Queretaro, Mexico (Siphonaptera)

Records of 40 species and subspecies of fleas (ectoparasites) found on indigenous rodents from the state of Queretaro, Mexico, are presented herein. Rodents were collected from 56 localities throughout the state. New flea records are given for hosts in two instances and new records for host localities in five instances.

The flea genus Sigmactenus (Siphonaptera: Leptopsyllidae): three new taxa from Sulawesi, updated identification key, and distribution map for all known species and subspecies.

One new species and two new subspecies of fleas are described. These are S. sulawesiensis n. sp. from North and Central Sulawesi, S. alticola pilosus n. ssp. from Central Sulawesi, and S. alticola crassinavis n. ssp. from North Sulawesi. All three of these new taxa are ectoparasites of native, endemic murine rodents. Two of the new taxa, S. sulawesiensis and S. alticola crassinavis, coexist on the same mountain, Gunung Moajat, in North Sulawesi. The related S. alticola alticola, which becomes the nominate subspecies, parasitises the murine rodent Maxomys alticola in northern Borneo (Sabah) and it is hypothesized that Sigmactenus first colonized Sulawesi as an ectoparasite of ancestral Maxomys, or perhaps Rattus, as these murines dispersed from southeast Asia to Sulawesi; 15 endemic murine rodent species belonging to these two genera are known to currently inhabit Sulawesi. An identification key and distribution map are included for all known species and subspecies of Sigmactenus. In addition to the three new taxa and S. a. alticola, these include: S. celebensis from South Sulawesi, S. timorensis from Timor, S. toxopeusi from New Guinea, and S. werneri from the Philippines (Mindanao and Negros).

Descriptions of Fleas from Northern Canada (Siphonaptera)

Three species and one subspecies of fleas, all belonging to the Ceratophyllidae, are described and figured in this paper. One of the species, formerly known only from Siberia, and imperfectly described, is redescribed, and recorded from Yukon Territory. The remaining two species and the subspecies are believed to be new to science.

Notes on Some Siphonaptera from Canada

In a previous paper on the Siphonaptera of Canada the writer (Holland, 1949b) summarized all available published and unpublished data up to December 31, 1946. One hundred and twenty-seven species and subspecies of fleas were recorded, of which 121 are believed to be endemic, and 6 introduced in historic times from the Palaearctic Region.Studies since 1946 have drawn attention to the occurrence in Canada of a number of species not hitherto recorded. Some of these were new to science. The discoveries of some of the others could have been predicted with reasonable certainty, the species being known from areas immediately south of the International Boundary, on species of hosts known to occur in Canada. In other instances the captures occasioned some surprise, the Canadian records representing rather broad extensions of the known ranges. Some of this supplementary information has already been published (Holland, 1949a, 1950, 1951a, 1951b). In the present paper, seven additional species and subspecies are reported from Canada for the first time. In partial compensation for this, the total of Canadian species is reduced by the recognition of two synonyms.