Flammulina Velutipes Polysaccharide Research Articles

Impact of Flammulina velutipes polysaccharide on properties and structural changes of pork myofibrillar protein during the gel process in the absence or presence of oxidation

The present study aimed to investigate the impact of Flammulina velutipes polysaccharide (FVSP) on the rheological properties and structural alterations of myofibrillar protein (MP) and oxidized MP (OMP), utilizing techniques such as rhehometer, fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). In the unoxidized system, the addition of 5.00% FVSP significantly improved (p < 0.05) the storage and loss moduli of the composite gel and promoted the α-helix to β-sheet transformation. These effects enhanced the protein's gel strength and water-holding capacity (WHC). In the oxidation system, 5.00% FVSP had significant effects (p < 0.05) on repair and improvement of the oxidized MP. These effects inhibited the cross-linking aggregation and degradation of the protein. In addition, the addition of FVSP significantly improved the gel properties of MPs after oxidation (p < 0.05), hindered fracture of the protein gel network structure. In summary, polysaccharides have a substantial effect on the functional characteristics of MP, and FVSP could potentially be applied in meat products.

Effects of Flammulina velutipes polysaccharide with ice recrystallization inhibition activity on the quality of beef patties during freeze-thaw cycles: An emphasis on water status and distribution

The antifreeze activity of Flammulina velutipes polysaccharide (FVP) autoclave-extracted with dilute alkaline and effects of FVP on moisture status, size of ice crystals, physical and chemical characteristics of beef patties during repeated freeze-thaw (F-T) cycles were investigated. Results showed that FVP exhibited ice recrystallization inhibition activity and was able to alter the onset freezing/melting temperature of beef patties. 0.01% FVP significantly alleviated (P < 0.05) the decrement in water holding capacity by inhibiting water migration, restraining the mobility of water, and reducing the size of ice crystals of beef patties during the repeated F-T cycles. In addition, FVP could effectively inhibited oxidation reaction and protein aggregation of beef patties with significant decreases in TBARS value, protein turbidity, contents of total sulfhydryl and carbonyl of myofibrillar protein, and an increase in protein solubility during the repeated cycles. These results suggest FVP could be developed to be a promising cryoprotectant in frozen patties.

Study on the physicochemical properties and antioxidant activities of Flammulina velutipes polysaccharide under controllable ultrasonic degradation based on artificial neural network

The polysaccharide fraction (FVP2) with molecular weight of 1525.09 kDa and intrinsic viscosity of 3.43 dL/g was isolated and purified from Flammulina velutipes (F. velutipes), and the ultrasonic degradation model of FVP2 was established to predict the molecular weight and intrinsic viscosity at the same time based on artificial neural network. FVP2U1 (1149.11 kDa, 1.78 dL/g), FVP2U2 (618.91 kDa, 1.19 dL/g) and FVP2U3 (597.35 kDa, 0.48 dL/g) with different molecular weights or viscosity were produced by this model to explore the effect of ultrasound on the physicochemical properties and antioxidant activity of FVP2. The results showed that ultrasonic treatment did not change the types of characteristic functional groups, monosaccharide composition and glycosidic bond of FVP2, but changed the chemical composition ratio and the degree of polymerization. Under ultrasonic treatment, the intrinsic viscosity of FVP2 still decreased significantly when the molecular weight did not decrease. Compared to other components subjected to ultrasonic degradation, FVP2U1 demonstrated higher molecular weight and viscoelasticity, while exhibiting lower antioxidant activity. In the case of no significant difference in molecular weight and monosaccharide composition, FVP2U3 with lower intrinsic viscosity has stronger hydration ability, higher crystallization index, lower viscoelasticity and stronger antioxidant capacity than FVP2U2.

Flammulina velutipes polysaccharides regulate lipid metabolism disorders in HFD-fed mice via bile acids metabolism

Our recent study demonstrated that the dynamic changes of gut microbiota mediated by Flammulina velutipes polysaccharide (FVP) could effectively regulate the lipid metabolism in high fat diet-fed (HFD-fed) obese mice model. In this paper, further research was carried out by examining the bile acid (BAs) profiles, as well as the BAs metabolic pathways changes in obese mice. Furthermore, the regulatory effect of BAs on lipid metabolism was verified by 3 T3-L1 preadipocyte differentiation model. The FVP administration resulted in lower BAs content in plasma of obese mice. From the qRT-PCR analysis, FVP could relieve cholestasis in obese mice through altering the BAs metabolic pathways, changing the related genes expressions in mice liver and ileum. The cholic acid (CA), chenodeoxycholic acid (CDCA), hyodeoxycholic acid (HDCA) and ursodeoxycholic acid (UDCA) were selected in cell experiment which all reduced the intracellular triglyceride content and increased the expression of AMPKα1 in 3 T3-L1 adipocytes. Furthermore, CA and CDCA were found increased the expression of PPARα. In combination with our previous research, we further confirmed in this paper that the changes of BAs metabolism caused by FVP showed a positive effect on lipid metabolism, both in obese mice and 3 T3-L1 adipocytes.

Effect of ultrasonic modification on the protective activity of Flammulina velutipes polysaccharide to prevent ethanol-induced injury on GES-1 cells

Flammulina velutipes (F. velutipes) polysaccharides were modified by ultrasound at the rated power of 150 W and 900 W. The monosaccharide composition, ultraviolet-visible, and Fourier transform infrared spectral characteristics of F. velutipes polysaccharides (FVP) and their ultrasonic modification products (U-FVPs) were determined. The protective effects of FVP and U-FVPs on human gastric mucosal cells GES-1 were confirmed for the first time. The mole ratios of glucose and galactose were decreased and the mole ratio of mannose was increased after ultrasonic modification. Compared with the original FVP and the FVP modified by ultrasound of 150 W (U-FVP1), the FVP modified by ultrasound of 900 W (U-FVP2) could better prevent ethanol-induced damage to GES-1 cells. With increasing ultrasound intensity, the protective effect of FVPs on GES-1 cells was significantly enhanced by more effective prevention of intracellular reactive oxygen species (ROS) production and more promotion of expression of triglyceride factor 2 (TFF2), prostaglandin E2 (PGE2), epidermal growth factor (EGF), and transforming growth factor β1 (TGF-β1) mRNA. The ultrasonic modification might be an effective way to develop novel F. velutipes polysaccharides that could effectively resist the gastric injury caused by excessive alcohol consumption.

Flammulina velutipes polysaccharide counteracts cadmium-induced gut injury in mice via modulating gut inflammation, gut microbiota and intestinal barrier

Cadmium (Cd), as Group I carcinogen, can induce damage to various organs including the gut. It is of great importance to meet the rising demand for effective therapies against Cd-induced damage and investigate the mechanism. Flammulina velutipes is a popular edible mushroom, despite the well-known health benefits of Flammulina velutipes, little is known about the effect of its polysaccharide (FVP) against CdCl2-intestinal injury. In this study, a FVP (uronic acid, 5.10 %; degree of methylation, 41.24 %) was produced via hot water extraction (85 °C) and ethanol precipitation. The FVP contained eight major monosaccharides and exhibited good thermal stability at temperatures lower than 139.73 °C. FVP (100 mg/kg b. w., gavage for 4 weeks) alleviated CdCl2 (1.5 mg/kg b. w., gavage for 4 weeks)-induced intestinal inflammation and apoptosis, intestinal permeability alteration and intestinal barrier disruption. FVP increased the abundance of Bacteroides, whilst decreasing the abundance of Desulfovibrionales and Clostridium. FVP also restored the levels of short-chain fatty acids (SCFAs), including acetic, propionic, isobutyric, butyric, isovaleric and valeric acids. Correlation analysis indicated the interplays among the FVP, gut microbes, SCFAs, intestinal barrier/cells and gut inflammation. FVP enhances the metabolic functions of gut microbiota via functional pathways analyzed by KEGG database. Furthermore, gut microbial transplantation of FVP + CdCl2 group mice partially alleviated CdCl2 caused-gut damage. Thus, FVP may be an effective therapeutic agent against CdCl2-induced gut damage via SCFA-mediated regulation of intestinal inflammation and gut microbiota-related energy metabolism. This study may open a new avenue for developing alternative strategies to prevent CdCl2-caused injury.

3D printing properties of Flammulina velutipes polysaccharide-soy protein complex hydrogels

Food 3D printing targets special consumers, such as children, elderly individuals and patients. For food 3D printing, soy protein isolate hydrogels are widely used but have obstacles in hydrogel reshaping. To improve the physical properties of the hydrogel for 3D printing, soy protein isolate can form composites with other hydrocolloids, such as Flammulina velutipes polysaccharide. Therefore, we evaluated the 3D printing accuracy and stability of the composite between soy protein isolate and Flammulina velutipes polysaccharide. The hydrogel of the composite with thermal treatment after 3D printing was accurate (>99.7%) and stable (>99.9%). Adding Flammulina velutipes polysaccharide and thermal treatment made the hydrogel less viscous and stronger, with a more uniform and denser microstructure. Thermal denaturing soy protein and introducing –OH from Flammulina velutipes polysaccharide to enhance β-sheets were the likely reasons for the improved hydrogel characteristics. Our findings suggested that adding Flammulina velutipes polysaccharide and thermal treatment could improve food 3D printing. • • Flammulina velutipes polysaccharide (FVP) improved the 3D printability of soy protein. • • Heated FVP and soy protein isolate (h-FVP-SPI) showed high accuracy via 3D printing. • • More β-sheets in h-FVP-SPI leaded to increased G′ and better stability. • • Strong networks of h-FVP-SPI leaded to a uniform surface and low free water.

Prevention of quality characteristic decline in freeze-thawed cultured large yellow croaker (Larimichthys crocea) using flammulina velutipes polysaccharide.

To investigate the cryoprotective effect of flammulina velutipes polysaccharide (FVP) on the quality characteristics in freeze-thawed cultured large yellow croaker, 0.050%, 0.075%, and 0.100% FVP was used before freezing and the quality after thawing was compared with water soaking (WS) and commercial cryoprotectant (CC) treatment. Quality attributes were comprehensively determined instrumentally and organoleptically after thawing at 4°C. Results showed that FVP effectively reduces the quality deterioration of body color and water-holding capacity, while no obvious effects were observed in texture and flavor. As for body color, both FVP and CC treatment could maintain the b* value to a large extent. Among them, 0.075% FVP shows the highest value in two sample points, with 55.2% and 21.0% increases seen in the values in WS. FVP-dose-dependent trends were found in water-holding capacity, where a reduction of 28.26% and 14.38% in thawing loss and cooking loss was observed in the 0.100% FVP group. Low-field nuclear magnetic resonance (LF-NMR) also revealed that immobilized water and free water were more tightly retained in the muscle tissue with FVP addition. The results of the sensory evaluation are essentially in line with the above observations. These findings indicate that FVP has the potential to partially replace commercial cryoprotectants in aquatic products during frozen storage.

Flammulina velutipes polysaccharide improves the water-holding capacity in the dorsal muscle of freeze-thawed cultured large yellow croaker (Larimichthys crocea)

To investigate the effect of Flammulina velutipes polysaccharide (FVP) on the water-holding capacity (WHC) in the dorsal muscle of freeze-thawed large yellow croaker, fresh fish were treated with FVP, commercial cryoprotectant (CC), and distilled water before freezing. The water-holding capacity (WHC) and related influencing factors were analyzed. The results showed that FVP was able to effectively improve the WHC by reducing thawing loss and cooking loss, and the effect was similar to that of the CC group. Smaller and rounder ice crystals and less damaged muscle tissue were observed in FVPs. FVP also significantly retarded the protein denaturation revealed by Ca2+-ATPase activity, ultraviolet–visible absorption spectra, and SDS-PAGE of the myofibrillar protein. Furthermore, protein oxidation was inhibited by FVP treatment, as demonstrated by the sulfhydryl group and intrinsic fluorescence intensity. In summary, FVP can effectively improve the WHC by inhibiting the growth of ice crystals, protein denaturation, and potentially protein oxidation.

The Effect of Flammulina velutipes Polysaccharide on Immunization Analyzed by Intestinal Flora and Proteomics

Proteomics and intestinal flora were used to determine the mechanism of immune modulatory effects of Flammulina velutipes polysaccharide on immunosuppressed mice. The results showed that compared with the model group, F. velutipes polysaccharide could increase thymus and spleen indices and improve thymus tissue structure in mice; IL-2 and IL-4 contents were significantly increased and IL-6 and TNF-α contents were significantly decreased; serum acid phosphatase (ACP), lactate dehydrogenase (LDH) and total antioxidant capacity (T-AOC) activities were increased (P < 0.05); in the liver, superoxide dismutase (SOD) and catalase (CAT) activities were increased (P < 0.001), while malondialdehyde (MDA) content was decreased (P < 0.001). Proteomics discovered that F. velutipes polysaccharides may exert immune modulatory effects by participating in signaling pathways such as immune diseases, transport and catabolism, phagosomes and influenza A, regulating the immune-related proteins Transferrin receptor protein 1 (TFRC) and Radical S-adenosyl methionine domain-containing protein 2 (RSAD2), etc. Gut microbial studies showed that F. velutipes polysaccharides could increase the abundance of intestinal flora and improve the flora structure. Compared to the model group, the content of short-chain fatty acids (SCFAs) and the relative abundance of SCFA-producers Bacteroides and Alloprevotella were increased in the F. velutipes polysaccharide administration group, while Lachnospiraceae_NK4A136_group and f_Lachnospiraceae_Unclassified decreased in relative abundance. Thus, F. velutipes polysaccharide may play an immunomodulatory role by regulating the intestinal environment and improving the balance of flora.

Effects of ultrasound on the degradation kinetics, physicochemical properties and prebiotic activity of Flammulina velutipes polysaccharide

The controllable ultrasonic modification was hindered due to the uncertainty of the relationship between ultrasonic parameters and polysaccharide quality. In this study, the ultrasonic degradation process was established with kinetics. The physicochemical properties and prebiotic activity of ultrasonic degraded Flammulina velutipes polysaccharides (U-FVPs) were investigated. The results showed that the ultrasonic degradation kinetic models were fitted to 1/Mt-1/M0 = kt. When the ultrasonic intensity increased from 531 to 3185 W/cm2, the degradation proceeded faster. The decrease of polysaccharide concentration contributed to the degradation of FVP, and the fastest degradation rate was at 60 °C. Ultrasound changed the solution conformation of FVP, and partially destroyed the stability of the triple helix structure of FVP. Additionally, the viscosity and gel strength of FVP decreased, but its thermal stability was improved by ultrasound. Higher ultrasonic intensity led to larger variations in physicochemical properties. Compared with FVP, U-FVPs could be more easily utilized by gut microbiota. U-FVPs displayed better prebiotic activity by promoting the growth of Bifidobacterium and Brautella and inhibiting the growth of harmful bacteria. Ultrasound could be effectively applied to the degradation of FVP to improve its physicochemical properties and bioactivities.

Effects of Flammulina velutipes polysaccharides on quality improvement of fermented milk and antihyperlipidemic on streptozotocin-induced mice

Flammulina velutipes polysaccharide (FVP) has become a focus of attention due to its extensive physiological activity, and the addition of polysaccharides to probiotic fermented milk is a hot research topic in functional dairy products in recent years. In the present work, the physicochemical property and bioactivities of fermented milk contained with FVP isolated from fruiting body were evaluated. Our results indicated that fermented milk contained with FVP had better qualities and sensory scores. Moreover, in vivo data manifested that fermented milk contained with FVP could ameliorate hyperglycemia against diabetes by reducing blood glucose and insulin levels, and regulating dyslipidemia and the disorder of glycometabolism via the PI3K/Akt signaling pathway. Taken together, the study demonstrated that fermented milk contained with F. velutipes polysaccharides might be bioactive compounds for diabetes prevention, providing the basic theory for developing novel fermented drinks.

The biological regulatory activities of Flammulina velutipes polysaccharide in mice intestinal microbiota, immune repertoire and heart transcriptome

The effects of a novel Flammulina velutipes polysaccharide (FVP) on intestinal microbiota, immune repertoire and heart transcriptome were investigated in this study. The results showed that FVP treatment could effectively regulate the abundance of colonic microbiota. And FVP exhibited obvious immunoregulatory effect by influencing V gene and J gene fragments usage on TCRα chain. The usage frequency of TRBV1, TRBJ1-6 and TRBJ1-5 were significantly altered, and 41 V-J pairs were identified with obvious difference after FVP treatment. Furthermore, the mRNA of mice heart was analyzed by transcriptome assay. Total 525 genes and 1587 mRNA were significantly changed after FVP treatment. KEGG annotation indicated that the up-regulated mRNA was enriched in 17 pathways including adherens junction, mTOR signaling pathway, insulin signaling pathway, mitophagy, tight junction, PPAR signaling pathway and TNF signaling pathway, etc. Meanwhile, the down-regulated mRNA was gathered in AMPK signaling pathway, metabolism of xenobiotics by cytochrome P450, apelin signaling pathway, PPAR signaling pathway, PI3K-Akt signaling pathway, insulin signaling pathway, cardiac muscle contraction, adrenergic signaling in cardiomyocytes, Fc gamma R-mediated phagocytosis, etc. The great potential exhibited by FVP could make it an ideal candidate as complementary medicine or functional food for promotion of health.

Flammulina velutipes polysaccharide improves C57BL/6 mice gut health through regulation of intestine microbial metabolic activity

Flammulina velutipes polysaccharides (FVP) can improve gut health through gut microbiota and metabolism regulation. In this study, the 28-days fed experiment was used to investigate gut microbime and metabolic profiling induced by FVP. After treatment, intestinal tissue section showed the higher villus height and villus height/crypt depth (V/C) value in FVP-treated group. The 16 s rRNA gene sequencing revealed microbiota composition alteration caused by FVP, as the Firmicutes phylum increased while Bacteroidetes phylum slightly decreased. The metabolic profiling was detected by LC/MS and results showed 56 and 99 compounds were dramatically changed after FVP treatment in positive and negative ion mode, respectively. Annotation in Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways displayed the adjustment of energy metabolism, amino acid metabolism, nucleotide metabolism and other related basic pathways after FVP treatment. Our study suggested that FVP can be developed as a dietary supplement for intestine health promotion.

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

The structural characteristics of a novel Flammulina velutipes polysaccharide (FVP2) were explored in this study. Besides, immunomodulatory activities of FVP2 on RAW 264.7 cell and its impact on gut microbiota in rats were investigated. FVP2 has a molecular weight of 18.3kD, and its main components include galactose (19.96%), glucose (60.66%) and mannose (19.38%). By NMR analysis, the main-chain structure consisted of (1→3)-linked-β-D-Gal, (1→6) -linked-β-D-Gal, (1→6)-linked-α-D-Glc and (1→3,6)-linked-α-D-Man was identified. Results of the in vitro assays on RAW 264.7 murine macrophage cells showed FVP2 could significantly up-regulate the expression of NO, TNF-α and IL-6. FVP2 was intragastrically administered to rats for 2weeks. Compared with the control group, two caecal short-chain fatty acids (SCFAs) concentration (isobutyric acid and butyric acid) and the abundance of beneficial microbiota of the FVP2-treated group were significantly increased (p<.05) respectively. The results demonstrated that FVP2 could effectively enhance the level of butyric acid and increase beneficial gut microbiota, which could improve the intestinal barrier function and maintain the intestinal mucosal integrity, suggesting that FVP2 could potentially be an immunomodulators or a functional food to promote intestinal health.

Effects of a β-type glycosidic polysaccharide from Flammulina velutipes on anti-inflammation and gut microbiota modulation in colitis mice.

Using the Flammulina velutipes polysaccharide (FVP) extracted from our previous study, herein, we investigated the improvement of this starch-free β-type glycosidic polysaccharide in alleviating dextran sodium sulfate-induced ulcerative colitis (UC) in mice. The absolute and relative abundance of intestinal microbes in the mouse feces were both determined by 16s RNA gene sequencing. The results from the histological analysis indicate that FVP treatment reduced the symptoms of UC, up- or down-regulated the relative gene expression levels in the colon tissue, and enhanced the capacity of metabolic and biogenesis in the UC mice, as predicted by PICRUSt. 11 species of gut microbes including Lactobacillus, Bifidobacterium, and Clostridium associated with UC symptoms were analyzed by correlation analysis. Our findings suggest that FVP can alleviate the UC symptoms in mice by regulating specific gut microbes, improving the understanding of the functional activity of FVPs as prebiotics.

In vitro and in vivo functional characterization of an immune activation Flammulina velutipes polysaccharide based on gut microbiota regulation

ABSTRACT Flammulina velutipes contains bioactive polysaccharides that can activate the immune system. Here we report the functional characterization in an immunomodulatory F. velutipes polysaccharide (FVP) both in vitro and in vivo. In cell experiment, FVP was discovered to be an activator to induce the M1-polarization of the RAW 264.7 cells. In animal experiment, FVP was found to raise the gene expression of IL-1β, iNOS and ZO-1 in mice colon tissue. Moreover, it seemed to achieve the immune regulative function by raising the relative abundance of Allobaculum, Lactobacillus, Alloprevotella, Akkermansia and Bifidobacterium. Gut microbiota also caused the body’s functional changes, enriched the harmful chemicals’ degradation ability and weakened the metabolism of carbohydrates. These findings suggest that FVP is an immunomodulatory polysaccharide that can boost cellular immune responses and a potential prebiotic agent in regulating gut microbiota.

Effects of ultrasound-assisted extraction on antioxidant activity and bidirectional immunomodulatory activity of Flammulina velutipes polysaccharide

In order to investigate the impacts of ultrasound-assisted extraction (UAE) on Flammulina velutipes polysaccharides (FVPs), the differences between FVPs extracted by ultrasound-assisted extraction (FVPU) and FVPs extracted by hot water extraction (FVPH) were compared in terms of yield, primary compositions, surface microstructure, helix-coil transition structure, molecular weight distribution, antioxidant activity, and bidirectional immunomodulatory activity. Results indicated that UAE changed the above properties of FVPs. Compared with FVPH, higher yield, protein content, and uronic acid content but lower polysaccharide and polyphenol contents were observed in FVPU. UAE changed the surface microstructure, destroyed the triple helix structure, and increased the proportion of low molecular weight polysaccharide components of FVPU. Compared with FVPH, FVPU showed a stronger reducing power and scavenging activities on DPPH radical, hydroxyl radical, and superoxide anion radical. FVPU was a better inhibitor of inflammation compared with FVPH. However, FVPH had a better immunity enhancing effect compared with FVPU. These results were attributed to the cavitation effect of ultrasonic waves on the structure of polysaccharides during the extraction process of UAE. These findings suggested that UAE was an efficient and environmentally friendly method to produce new polysaccharides from F. velutipes for the development of functional foods or nutraceuticals.

Effects of Flammulina velutipes polysaccharide on immune response and intestinal microbiota in mice

Abstract A crude polysaccharide derived from Flammulina velutipes (FVP), mainly consisting of glucose linked with β-glycosidic bonds, was administered to 6 week-old male ICR mice by gavage for five weeks, to investigate the effects on mice intestinal microbiota and immune response. The pH of the intestinal compartments decreased while short chain fatty acids concentrations increased in FVP-fed groups, in a dose dependent manner. High throughput sequencing revealed the enrichment in the diversity and an alteration in the composition of the fecal microbiota in the FVP fed mice. A significant decrease in the Firmicutes/Bacteroidetes ratio, relative abundances of Lactobacillus and Akkermansia were related to the energy absorption. The relative abundances of Porphyromonadaceae and Bacteroidaceae positively correlated with an increase in serum immunoglobulin and cytokines. This study indicated that this crude polysaccharide from F. velutipes could modulate the gut microbiota and represented a potential capacity of obesity suppression and immune regulation.

Preparation, characterization, and anti-inflammatory effect of the chelate of Flammulina velutipes polysaccharide with Zn

ABSTRACTThe optimal conditions for preparing Zn-Flammulina velutipes polysaccharide (Zn-FVP) chelate were studied by using L9 (34) orthogonal test. The results showed that the optimized conditions for the chelating reaction were as follows: initial concentration of Zn (II), 6 mg/mL; ratio of purified polysaccharide (FVPp) to Zn (II), 1:2; chelating time, 8 h; and pH, 5. Chelating rate achieved 98.11% under the optimal conditions. Characteristic bands for OH stretching at 3000–3500 cm−1 and bands corresponding to C-H variable angle vibration between 1000–1100 cm−1 showed some obvious differences for chelation of polysaccharides and zinc. The qualitative spectrum of Zn-FVP indicated the presence of ZnSO4 and the abundant Zn (II) groups were widely present in the chelated samples. The anti-inflammatory effect of FVPp and Zn-FVP on Lipopolysaccharide-stimulated RAW 264.7 cells was evaluated. In the range of 5–50 μg/mL, Zn-FVP did not exhibit obvious inhibition of the survival rate of cells. Zn-FVP in noncytotoxic levels showed strong suppressive effect on the inflammatory cytokines (IL-6, TNF-α, INF-γ and NO) and these provided a theoretical base for the anti-inflammatory mechanism of Zn-FVP.