ABSTRACT The oyster Striostrea prismatica is a fishery-relevant species in the eastern tropical Pacific, particularly in the Mexican Central Pacific (MCP), and little is known about its reproductive cycle. Therefore, our study analysed gametogenesis, sex ratio, sexual maturity stages, shell height at first maturity, and condition index during 2014 and 2015 in El Tizate and La Victoria in the MCP. The sex ratio was significantly biased towards males at both sites, presumably due to ontogeny or fishing pressure. The highest condition index was observed between April and May in both years, while lower values were observed from September to December. The reproductive cycle in both sites was annual, with maturity and spawning from June to August and the degradation phase in August. The condition index showed a negative correlation with temperature and chlorophyll a in both sites: as a result, the period of maximum maturity and spawning coincided with the closure of the fishery in Mexico (June-August). Females had more significant shell height at first maturity than males at both sites, possibly associated with fishing pressure. The information from this study is a significant contribution, but it is necessary to extend the studies to populations further south and further north.