Fish Aquaculture Research Articles

A Global Synthesis of Environmental Enrichment Effect on Fish Stress

ABSTRACTThe stress‐coping ability (SCA) is one of the core aspects of fish welfare and is of vital importance for fish production in the aquaculture industry and for fish fitness in hatchery release. Environmental enrichment (EE), a method of introducing external stimuli into the husbandry environment, has been recently proposed to improve fish SCA, but the present experimental evidence is mixed, and the reasons for these discrepancies are unclear. Here, we conducted a global meta‐analysis using a data set that consists of 1786 cases from 107 studies across 42 fish species to solve this problem. Overall, we found that enriched fish had significantly higher SCA than control fish, reflected in either basal stress levels or stress responses. Meta‐regression analyses showed that specific subgroups of enrichment type, fish developmental stage, stress category, stress duration, stress place, sample tissue and indicator system showed significant positive EE effects on SCA. Multi‐model inference indicated that the indicator system, fish developmental stage, stress characteristic and enrichment mode are important drivers for the high heterogeneity among effect sizes. These results highlight the importance of introducing EE into the rearing systems, which will not only increase the welfare of aquaculture fish but also improve the ecological adaptability of released fish. The comprehensive knowledge obtained in this analysis will provide insights into fish ontogenetic plasticity and its responses to EE and have important implications for improving the production cycle in fish aquaculture and fisheries conservation.

Using a length-weight relationship based on wild lumpfish (Cyclopterus lumpus L.) for estimation of body condition of lumpfish in salmon cages.

The use of lumpfish (Cyclopterus lumpus L.) as cleaner fish in Atlantic salmon aquaculture has emerged as a promising solution to combat the issue of sea lice infestation, as they have proven effective under the right conditions. The aquaculture industry, however, is facing challenges in ensuring the welfare and survival of lumpfish in salmon cages. Part of the welfare monitoring of lumpfish is the estimation of body condition. Previous research on standard length-weight relationships for lumpfish has primarily relied on the growth patterns of lumpfish in salmon cages, which might not align with the natural growth patterns of the fish. In this study, we argue for another perspective: using the standard weight of wild lumpfish to estimate the body condition of lumpfish in salmon cages. We assert that this approach aligns more closely with the natural growth pattern of the fish and may offer a more accurate representation of their condition. Our findings show significant differences in growth patterns between wild lumpfish, which show positive allometric growth, and those in salmon cages, which show slightly negative allometric growth. The findings underscore the importance of using appropriate length-weight relationships for lumpfish in aquaculture to ensure an accurate assessment of their body condition.

Common Carp (Cyprinus carpio) Fish Aquaculture Revitalization, Multiplication and Sustainability in Nigeria

Thirty three mature broodfish of Common Carp were harvested from Panyam Fish Farm, Plateau State (PFFPS), and from Federal University of Agriculture Makurdi Research Fish Farm (FUAMRFF). These farms are situated in two different agro-ecological zones of Nigeria (with a fading history of Carp fish culture). The broodfish were fed for 90 days, in preparation for on-farm breeding activities with the aim of determining water quality, hatching parameters (through un-induced natural spawning, induced natural spawning and induced breeding by stripping in indoor concrete ponds); thirdly, to determine percentage survival of the bred Carp fish progenies cultured for 56 days (in ponds sampled in PFFPS and FUAMRFF). Fourthly, to evaluate growth and potential economic returns from the two areas, and finally to further evaluate fish farmers perception of Carp fish culture in selected fish farms across other agro-ecological zones in Nigeria. After breeding activities, progenies were cultured and fed to satiation. The water quality parameters in the ponds and in water holding tanks was monitored daily. At the end of the research period, PFFPS showed better Carp fish hatching tendency, highest (94%) in Induced Natural spawning), with better environmental condition and water physico-chemical parameters for Carp fish culture with mean Dissolved Oxygen content level (8.35 Mg/l), and pH (7.54). Furthermore, PFFPS has capacity to accommodate the mass production of Carp fish. Economically, PFFPS had a higher gross margin (402,250), and Profit index (5.0). Also, result from fish farmers perception on carp fish culture showed that 62% of farmers have no idea of Carp fish management, 66% have no idea of Carp fish selling price, 66% are aware of PFFPS and 100% expressed that more awareness should be created on aquaculture diversification (with interest in Carp fish aquaclture), as this will meet consumer demand and rescue this species from its current track of going extinct in Nigeria, if given urgent attention).

Can commercial aquafeeds improve the nutritional value of brine shrimp? Proximal composition and lipid profile of alternative enhancers

Artemia, commonly known as brine shrimp, holds a significant role as live prey in ornamental and commercial fish aquaculture. This study addressed the nutritional deficiency in adult Artemia biomass, exploring alternative enrichers available in Brazil. Evaluating a commercial aquafeed (Polinutre Poli Camarão 400PL) and a lipid emulsion (BioViv HUFA Continum), adult Artemia were subjected to different enrichment protocols, including periods of 1, 12, and 24 hours with each enricher, as well as a test group raised 100% on the commercial feed. Protein content for Artemia exclusively fed the commercial aquafeed (CF100) reached values very similar to those provided by the main commercial products available worldwide and other commonly used live prey. Enrichment time effects were observed from 12 h onwards, showing positive impacts on protein accumulation. Lipid content peaked at 12 h, followed by a decrease at 24 h. Further studies are needed to assess whether the combination of feed-based protocols followed by 12-h lipid emulsion enrichment can surpass the current results, providing high levels of protein content while maintaining a complete lipid profile.

A review of deep learning-based stereo vision techniques for phenotype feature and behavioral analysis of fish in aquaculture

The industrialization, high-density, and greener aquaculture requires a more precise and intelligent aquaculture management. Phenotypic and behavioral information of fish, which can reflect fish growth and welfare status, play a crucial role in aquaculture management. Stereo vision technology, which simulates parallax perception of the human eye, can obtain the three-dimensional phenotypic characteristics and movement trajectories of fish through different types of sensors. It can overcome the limitations in dealing with fish deformation, frequent occlusions and understanding three-dimension scenes compared to the traditional two-dimensional computer vision techniques. With the deep learning development and application in aquaculture, stereo vision has become a super computer vision technology that can provide more precise and interpretable information for intelligent aquaculture management, such as size estimation, counting and behavioral analysis of fish. Hence, it is very beneficial for researchers, managers, and entrepreneurs to possess a thorough comprehension about the fast-developing stereo vision technology for modern aquaculture. This study provides a critical review of relevant topics, including the four-layer application structure of stereo vision technology in aquaculture, various deep learning-based technologies used, and specific application scenarios. The review contributes to research development by identifying the current challenges and provide valuable suggestions for future research directions. This review can serve as a useful resource for developing future studies and applications of stereo vision technology in smart aquaculture, focusing on phenotype feature extraction and behavioral analysis of fish.

Water Quality Impact on Fish Behavior: A Review From an Aquaculture Perspective

ABSTRACTChanges in water quality significantly shape fish behavior, a crucial index reflecting the growth and welfare status of fish. Given the centrality of this relationship to aquaculture practices, a comprehensive understanding of how water quality dynamics influence fish behavior is imperative. While there have been some summaries of the effects of water quality parameters on fish physiology and growth, few reviews on their effects on fish behavior have been reported yet. This article reviews several water quality parameters which are of great concern in aquaculture from multiple facets of actual production, including physical parameters (water temperature and turbidity), chemical parameters (dissolved oxygen, salinity, pH, and inorganic nitrogen), and chemical pollutants (microplastics and crude oil), which have gained increasing attention from the researchers and aquaculture practitioners over the past decades. Variations in these water quality parameters can exert profound effects on fish physiology, metabolism, internal tissues and organs, and sensory perception, which influences fish behaviors such as swimming, schooling, feeding, predation, anti‐predation, aggression, courtship, as well as adaptive and stress‐related behaviors such as exploration, avoidance response, and anxiety‐like behavior. By synthesizing the behavioral changes caused by specific water quality parameters, this review aims to provide strong support for further water quality‐related research, thereby fostering environments conducive to both fish welfare and aquaculture productivity.

Claudin2 is involved in the interaction between Megalocytivirus-induced virus-mock basement membrane (VMBM) and lymphatic endothelial cells

The genus Megalocytivirus, belonging to the family Iridoviridae, is one of the most detrimental virus groups to fish aquaculture. Megalocytivirus creates a virus-mock basement membrane (VMBM) on the surface of infected cells. This membrane provides attachment sites for lymphatic endothelial cells (LECs), disrupting fish's endothelial cell-extracellular matrix system. This disruption triggers injury to the vascular system and can result in death. Exploring the VMBM-cell interaction mechanism is crucial for uncovering the pathogenesis of Megalocytivirus and identifying therapeutic targets. Claudins, a class of tetra transmembrane proteins, play a key role in creating tight junctions between endothelial or epithelial cells. In this study, we demonstrated that the expression of Claudin2, a member of the Claudin family in fish, was significantly up-regulated by Megalocytivirus infection. Claudin2 was found in LECs attached to the surface of infected cells. It interacted with the VMBM viral components VP23R, VP08R, and VP33L at multiple binding sites through its two extracellular loops. However, it did not interact with the host basement membrane’s nidogen. Therefore, Claudin2 is involved in the interaction of LEC with VMBM and plays a role in the disturbed distribution of extracellular matrix and endothelial cells in Megalocytivirus-infected fish tissues. This study aims to uncover the molecular mechanisms by which Megalocytivirus infection leads to pathological changes in the vascular system.

Comparative Genomic Analysis Reveals Distinct Virulence and Resistance Mechanisms in 21 Bacterial Fish Pathogens

The rising bacterial infections threaten world aquaculture and wild fish populations, making it imperative to increase the understanding of the mechanisms of pathogenic virulence and resistance. This study applies comparative genomic analysis to 21 bacterial fish pathogens, using whole-genome sequences from public genomic resources and sophisticated bioinformatics tools for screening of virulence factors, mobile genetic elements, antibiotic resistance genes, anti-phage defense mechanisms and secretory systems. We have seen that the different pathogens depict a wide range of variability regarding virulence and resistance potential, which may be attributed to species-specific adaptation. Notably, Streptococcus agalactiae and Mycobacterium salmoniphilum were found to possess high offensive and defensive virulence potential, but at different regulative controls. We also found diverse secretion systems and intricate mechanisms for antibiotic resistance, which have provided very important insights into how pathogens adapt to their environments. By categorizing functional genes and finding anti-phage systems, our analysis has revealed new insights into the complex interactions among bacterial virulence, resistance, and host defense mechanisms. These findings not only shed new light on the bacterial pathogenesis process in aquaculture but also provide the bases for focused, therapeutically-based strategies and genomic surveillance programs able to improve disease management and sustainability in aquaculture environments.

Bacillus amyloliquefaciens SS1-fermented soybean meal influenced the flesh texture of Nile tilapia: Involvement of microbial metabolite propionate

Microbial fermentation is recognized to improve feed utilization and health of farmed animals. However, its effect on flesh quality and the underlying mechanisms remain understudied. Herein, Bacillus amyloliquefaciens SS1, a probiotic strain isolated from the intestinal tract of Nile tilapia (Oreochromis niloticus) was used to ferment soybean meal (FSM). Two tilapia experimental diets containing FSM and raw soybean meal were formulated for an eight-week feeding trial. Results showed that dietary FSM significantly increased the carcass ratio of fish, while there was no significant difference in weight gain or feed utilization between the two dietary treatments. Texture profile analysis showed that muscle springiness was improved by the FSM diet. There was no discernible difference in myofiber morphology between the two treatments. However, the FSM-fed fish had significantly lower muscle lipid contents than the control, accompanied by suppressed lipogenesis, while lipolysis was promoted both in muscle and liver tissues. Interestingly, the FSM diet modified gut microbial community, resulting in increased propionate concentrations. By establishing a high-fat model using primary hepatocytes of tilapia, we demonstrated the role of propionate in reducing lipid accumulation. Our finding highlights the emerging roles of gut-derived metabolites in affecting flesh quality by modifying host metabolism, providing a foundation for improving the flesh quality of aquaculture fish starting from the feed.

Microplastic contamination in the aquaculture icon Oreochromis mossambicus: Prevalence, characteristics, and comprehensive overview

The global production of plastics has surged to 368 million tonnes annually, leading to significant plastic waste accumulation, projected to reach 12,000 Mt by 2050, impacting aquatic ecosystems. Fish, crucial for their protein and nutrients, are particularly vulnerable to microplastic (MP) ingestion. As a major aquaculture producer and fish consumer, India faces rising plastic pollution in freshwater, which disrupts fish health and growth, posing a significant threat to the sustainability and productivity of aquaculture systems and potential health risks. This study focuses on Mozambique tilapia (Oreochromis mossambicus), valued for its widespread aquaculture use, rapid growth, and nutritional benefits. Our research reveals significant MP contamination (69.23 %) in Mozambique tilapia, with over 80 % from the Ulhas River and 60 % from the Bhima River contaminated and females showing higher susceptibility. These findings emphasise the need for further research on MP impacts on human health and the development of mitigation strategies.

Exploring Botanical Innovations in Fish Aquaculture: A Review of Biological Impacts and Industry Prospects

Culture fisheries play a crucial role in providing protein-rich sustenance for the expanding global population. However, the intensification of fish farming comes with a downside – heightened stress among fish, which subsequently escalates the risk of disease transmission. The emergence of resilient bacterial populations and the accumulation of residues in body tissues due to the use of drugs and chemicals have underscored the necessity for the development of environmentally friendly and durable solutions. Notably, there has been a recent upsurge in interest regarding the use of botanical products to enhance aquaculture practices. Plant derived secondary metabolites have demonstrated their efficacy in stimulating appetite, facilitating growth, boosting immune responses, and exhibiting antibacterial as well as anti-parasitic properties within the realm of aquaculture. This article aims to comprehensively examine the body of research on the application of plant-derived products in pisciculture, elucidating their effects on various biological aspects of fish, including growth patterns, immune system functionality, pigmentation, blood composition, and reproductive parameters. Furthermore, the article also delivers inputs into an assessment of the current status of these practices, the methodologies deployed, and the challenges encountered in integrating plant-based solutions into the aquaculture industry.

Mandarin Fish Ranavirus (MRV) Infection Induced Inflammation and Histologic Lesions in the Gut of Mandarin Fish.

Mandarin fish ranavirus (MRV) is widely spread in China and causes huge economic losses to the mandarin fish (Siniperca chuatsi) aquaculture. However, the pathogenesis of MRV is still unclear. In the present study, mandarin fish were artificially infected with MRV, and then different gut compartments from diseased fish were subjected to histologic analysis by H&E staining, quantification of proinflammatory genes and MRV copies by qPCR. MRV-MCP protein expression was assessed using indirect fluorescence assay (IFA) and immunohistochemistry. Proliferation of IgM+ B cells was evaluated by indirect fluorescence assay (IFA). Then, we found that MRV infection caused serious histologic lesions along with inflammatory cell infiltration, especially in the foregut. A significant accumulation of IgM+ B cells was detected in the foregut (~6.5-fold) and hindgut (~3.3-fold), respectively. The expression of inflammation-related genes such as IL-1β, IL-6, IL-8,TNF-α, CSF1r and NCF1 was significantly upregulated in the foregut, varying from ~2.8-fold to ~11.9-fold. In addition, MRV exhibited foregut tropism, according to the investigation of viral loads and MCP protein expression. Overall, our findings indicated that MRV-induced hyperinflammation in the gut eventually led to enteritis. This study provided new insights into uncovering the pathogenesis of MRV infection.

Phylogenetic and Genetic Diversity of the Spiny Eel Mastacembelus unicolor (Mastacembelidae) from Kediri, East Java, Indonesia

The spiny eel from the family Mastacembelidae are potential fish for aquaculture because it’s high economic value as an ornamental and as a consumption fish. Unfortunately, this fish have not been cultivated yet and up to present local people depend on wild-caught. This situation leads to severe population decline of this species in nature. Efforts to cultivate or protect this species in nature are therefore crucial. Species identification is the first and essential step performing domestication or conservation of a species. In this study, we conducted morphological identification as well as phylogenetic and genetic diversity analyses using cytochrom c oxidase subunit I gene. The results revealed spiny eel from Kediri can be identified as Mastacembelus unicolor based on both morphological and molecular analyses. The phylogenetic analyses suggested that individuals from Kediri have the closest relationship to M. unicolor individuals from West Java. Individuals sequenced from Kediri exhibit identical haplotype which indicating no genetic polymorphism between individuals. The individuals of Mastacembelidae in the Kediri were morphologically and genetically identified as Mastacembelus unicolor. The genetic analysis result shows Kediri have 53.13% AT, 48.67% GC, and one haplotype with low hd and low π value.

Assessment of Plastic Waste Generation and Environmental Leakage in Hai An Ward, Nghi Son Town, Thanh Hoa Province

The study estimates plastic waste generation and leakage to the environment and the sea in Hai An ward, Thanh Hoa province. The study uses a material flow analysis model, field samplings (include plastic waste sorting and weighing), and secondary data collection and analysis. The plastic waste generated is estimated to be 77.01 ton/year, of which the plastic waste from domestic sources and other sources, including agriculture and aquaculture (fishing), are 71.17 ton/year and 5.84 ton/year, respectively. Research results show the composition of plastic waste from domestic sources is diverse, with bottles being the highest (28.4%), followed by plastic bags (23.1%). The findings of the study also indicate that the plastic waste leakage to the environment is 9.63 ton/year, of which 6.4 tons are from the uncollected plastic waste and 3.23 tons are the plastic waste lost during the collection, transportation, and treatment process. The research also estimates that the plastic waste leakage to the sea accounts for about 7.24 ton/year, plastic waste retained on land is about 2.16 ton/year, and the plastic waste openly burnt is 0.23 ton/year.

Cultivation of Aurantiochytrium sp. Using Pickle Seasoning Liquid Waste and Application of the Raw Biomass for Fish Aquaculture

AbstractAurantiochytrium sp. strain L3W is a heterotrophic microorganism that produces docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), which are essential for the growth of marine fish. In this study, pickle seasoning liquid waste was used for culturing strain L3W and the raw biomass of strain L3W was then fed to red sea bream fingerlings (Pagus major) to confirm its usability as a source of DHA and EPA. The strain L3W was cultured on the three pickle seasoning liquid waste samples, Akimurasaki, Hiroshimana, Shisohijiki, diluted with sand-filtered seawater at initial pH values of 4 and 7 under unsterile conditions. The growth of strain L3W was highest at 3.71 g/L on Hiroshimana at an initial pH of 7 with DHA and EPA production at 71.4 and 6.8 mg/g-biomass, respectively. Preparing the raw biomass of strain L3W by the 200 L-scale cultivation using the American Type Culture Collection 790By+ medium because of the DHA and EPA contents close to that produced in the Hiroshimana medium with an initial pH of 7, the raw biomass was spiked to the diet at 3%, 5%, and 10%. The final DHA and EPA contents in the whole body were increased by 4.26 and 3.03 times, respectively, by adding the strain L3W biomass at 10%. These results confirmed the feasibility of a carbon cycling scenario in which the strain L3W is cultivated using liquid food waste with the resultant biomass is utilized as a source of DHA and EPA for fish aquaculture. Graphical Abstract

Determining the Consequences of Climate Change for Aquatic Ecosystems Using Bioassay Methods: a Review

The article describes the scientific and methodological possibilities of bioassay in the field of studying the effects of climate change on water bodies and aquatic organisms. In fish aquaculture, it was revealed that an increase in water temperature changes the biochemical parameters of the internal environments of organisms, affects their behavior and the number of offspring. A number of biogeochemical transformations of aquatic ecosystems are predicted: a decrease in the pH of water, mineralization of organic matter of bottom sediments, release and increase in bioavailability of compounds of potentially toxic elements. Bioassay methods using monocultures and laboratory microcosms have shown that maximum temperature values and a cascade of concomitant changes will lead to a restructuring of the aquatic life community, changes in the habitats of organisms, and the disappearance of stenothermic species. The realism of such scenarios is confirmed by paleodata and modern natural phenomena.

Fish resilience as an ethical issue.

Fish resilience can be understood as the capacity of fish to successfully respond to a challenge so that they are able to function and flourish in much the same way as they did prior to the occurrence of the challenge. Resilience is a function not only of individual fish, but also of a whole fish population. Enhancing the resilience of fish requires both adapting the robustness of the animals and adapting the (production) environment to the specific needs of the fish. Rather than a mere biological capacity of fish, resilience also comes with ethical questions. These questions occur at four levels. First, in practice resilience often comes with a "rhetoric" of optimalization. The view that aquaculture that strives for resilient fish is good for both fish welfare and production is inherently normative. It assumes a 'win-win situation', but thereby makes certain normative assumptions. Second, especially when the win-win situation is not achievable, resilience means making trade-offs between preferred responses to challenges from the perspective of individual animals and groups or between individual housing and larger aquaculture systems. Third, the discussions on resilience and fish demonstrate the need to move beyond an animal welfare framework when discussing the treatment of fish in aquaculture. Recently, animal ethics has seen a turn towards centering animals' own agency. This means that we should not only focus on improving animal welfare, but also on asking what the animals themselves want and how they can be given more control over their situation. This may also impact the definition of resilience and how it is made operational. Finally, the use of the concept of resilience may reveal a certain moral outlook with regard to fish. On the one hand, resilience is portrayed as a positive characteristic of animals that enables improvement of the quality of life of fish. At the same time, it raises the question of how far we should stretch the "manufacturability" of fish. When we physically adapt animals so that they can cope with difficult circumstances we may be stretching moral boundaries. For example, this raises the objection that we are instrumentalizing animals. In this article, we reflect on these types of ethical issues and aim to show that the ethical dimensions of resilience need to be taken into account by professionals in aquaculture in order to make resilience operational and to contribute to a responsible interaction with fish in aquaculture.

Economic Impact of Heavy Metals Accumulation in Aquacultured Tilapia Fish in Egypt

Economic Impact of Heavy Metals Accumulation in Aquacultured Tilapia Fish in Egypt

Reproductive performance of lumpfish (Cyclopterus lumpus, L. 1758) females: Effects of integrated photoperiod and temperature manipulations on sexual maturation and spawning.

A successful control of sexual maturation is crucial for year-round production of lumpfish juveniles destined as cleaner fish in Atlantic salmon aquaculture. This study investigated the combined effects of photoperiod and temperature manipulations on sexual maturation and spawning in lumpfish females. Lumpfish juveniles were exposed to simulated natural and nine-month compressed annual photoperiods, with subsequent temperature elevation. Body weight (BW), condition factor (K), gonadosomatic index (GSI), ovarian development, plasma levels of 17β-estradiol (E2), testosterone (T) and 11-ketotestosterone (11-KT), and spawning were assessed. Compressing the natural photoperiod caused a clear increase and decrease in GSI, T, 11-KT and E2 towards and during the spawning period. Before the temperature elevation, GSI, T, 11-KT, E2 and ovarian development were advanced in the compressed photoperiod. After the temperature elevation, GSI, T, 11-KT and E2 fluctuated more in the compressed photoperiod, while in the natural photoperiod, E2 declined, and GSI, T and 11-KT increased. Spawning was advanced by 1 month in the compressed photoperiod compared to the natural photoperiod. Temperature elevation led to higher levels, earlier peaks and declines of T, 11-KT or E2 in both photoperiods, and advanced spawning by 1.5 months in the compressed photoperiod compared to the natural photoperiod. Temperature elevation also led to increased ovulation recruitment and increased cumulative weight of spawned eggs in the natural photoperiod. Compressing the natural photoperiod and elevating temperature can thus advance sexual maturation and spawning in lumpfish females. Due to the lower amounts of spawned egg weights in the high temperature compressed photoperiod, further studies on effects of photoperiod and timing of temperature manipulations on spawning, fecundity and egg quality could optimize the photothermal manipulations on lumpfish broodstock.

Integrated miRNA-mRNA analysis uncovers immediate-early response to salinity stress in gill-derived cell line of Gymnocypris przewalskii

Salinity adaptation is an important issue in aquaculture. Understanding the immediate-early response to salinity stress helps in comprehending this process. In vitro experiments using cell lines can explain cell-independent reactions without the involvement of hormones in vivo. In this study, salinity stress experiments were conducted using cell line derived from the gills of Gymnocypris przewalskii (GPG cell line) to isolate immediate-early response-related genes and miRNAs using transcriptomics, followed by bioinformatics analysis. The results showed that intracellular free Ca2+ appeared to be a key factor in cell sensing and initiating downstream cell signaling in response to external salinity. Additionally, cell apoptosis was the most common feature of salinity stress, with multiple signaling pathways involved in salinity-induced cell apoptosis. Furthermore, MiRNAs played a crucial role in the rapid response to salinity stress by selectively inhibiting the expression of specific genes. Additionally, for the first time in the G. przewalskii genome, Tf2 and TY3 families of transposons were found to have responsive roles to the external salinity stress. This study contributes to a better understanding of osmotic sensing in G. przewalskii and provides theoretical assistance for improving salinity adaptation in aquaculture fish species.