In this paper we define a cohomology theory for an arbitrary K-linear semistrict semigroupal 2-category ( C,⊗) (called for short a Gray semigroup) and show that its first-order (unitary) deformations, up to the suitable notion of equivalence, are in bijection with the elements of the second cohomology group. Fundamental to the construction is a double complex, similar to the Gerstenhaber–Schack double complex for bialgebras, the role of the multiplication and the comultiplication being now played by the composition and the tensor product of 1-morphisms. We also identify the cohomologies describing separately the deformations of the tensor product, the associator and the pentagonator. To obtain the above results, a cohomology theory for an arbitrary K-linear (unitary) pseudofunctor is introduced describing its purely pseudofunctorial deformations, and generalizing Yetter's cohomology for semigroupal functors (in: M. Kapranov, E. Getzler (Eds.), Higher Category Theory, AMS Contemporary Mathematics, Vol. 230, Amer. Math. Soc., Providence, RI, 1998, pp. 117–134). The corresponding higher order obstructions will be considered in detail in a future paper.
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