To address the difficulty in conducting fire tests to verify the fire-resistance limit of large one-way slabs with heavy loads in a metro, a scale fire test method is proposed based on the bearing capacity calculation of the one-way slab under fire. The scale fire test method adapted the hypothesis that the deflection of the one-way slab under fire is close to a half-sine function and the plane section hypothesis. The validity of this hypothesis is verified through fire tests and finite element simulations. The scale fire test method achieves a similar temperature field and mechanical behavior between the scaled model and full-scale model of the one-way slab. The results of the fire tests showed that the temperature field and mechanical behavior of the scaled model were consistent with those of the full-scale model, with an error in fire resistance of 4.7%. The calculation results and fire test results are essentially consistent, with an error of 6.5%, and according to the calculation of the one-way slab fire-resistance limit, the key factor affecting the fire resistance of the one-way slab under fire is the temperature of the bottom rebars. Using the scale fire test method, the size effect of the one-way slab under fire still exists, and larger slabs have a greater deformation capacity.